BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0152.p (311 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310460|ref|YP_003774552.1| D-3-phosphoglycerate dehydrogen... 238 4e-64 gi|300311801|ref|YP_003775893.1| D-isomer specific 2-hydroxyacid... 118 5e-28 gi|300313156|ref|YP_003777248.1| D-2-hydroxyacid dehydrogenase [... 92 4e-20 gi|300310832|ref|YP_003774924.1| D-isomer specific 2-hydroxyacid... 83 3e-17 gi|300310960|ref|YP_003775052.1| dehydrogenase oxidoreductase [H... 79 4e-16 >gi|300310460|ref|YP_003774552.1| D-3-phosphoglycerate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 308 Score = 238 bits (607), Expect = 4e-64, Method: Compositional matrix adjust. Identities = 125/312 (40%), Positives = 183/312 (58%), Gaps = 5/312 (1%) Query: 1 MRILFQTADPANQDAWLRDLRTALPHAGIRVW-QSGDTAAADYAIVWKPPAEMLQGRPEL 59 M L++ ADP W + P ++W GD A+ Y W PP ++ + P+L Sbjct: 1 MTFLYK-ADPVRGAEWAQLFAQKAPELPFQIWPHDGDGASVRYLAAWTPPDDIAERFPKL 59 Query: 60 KAVFNLGAGVDAILKLNVVPDTLPLIRIDDGGMAVQVAEFATHAVLRYYRRLDQYERQAR 119 + +F++GAG+D ++ +P LP++R+ + G+A + ++ THAVL +R Y RQ R Sbjct: 60 EILFSVGAGIDQ-FDMSTLPPALPVVRMTEPGIAAGMVQYVTHAVLGLHRDSRVYARQQR 118 Query: 120 SNGGEWKPLPPFERSGFGIGVLGLGVLGQRICESLQHFGFEVSGWSRSPKTLPGVKAYAG 179 W+ S +GVLGLG+LG+ + + L GF +GWSRS + G+ Y+G Sbjct: 119 DQ--VWRAHRVRPASACRVGVLGLGMLGRAVLQQLHGLGFPCAGWSRSAHQMDGIDCYSG 176 Query: 180 SAQLNEFLRNARVLICMLPLTPDTAGILNRDTLSQLPRGAYLINLARGGHLVEADLPPLL 239 SA L FL VL+C++PLT +T G+L RD +QLPRGA LIN+ RGGHLVE DL L Sbjct: 177 SAGLRAFLARTDVLVCLVPLTEETRGLLCRDLFAQLPRGAALINVGRGGHLVEDDLLAAL 236 Query: 240 ESGQIAGATLDVFQEEPLPPHHPFWKDPRIAITPHISALTVRSEAARQIAEKIAALQRGE 299 GQ++ A LDV + EPLPP HPFW P I +TPHI+++T A + E + + G Sbjct: 237 NDGQLSAAVLDVCEVEPLPPGHPFWTHPDIVLTPHIASMTQPDGAVDAVLENLRRYREGL 296 Query: 300 AVVGLVDRNKGY 311 ++GL+DR +GY Sbjct: 297 PLLGLIDRQRGY 308 >gi|300311801|ref|YP_003775893.1| D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding protein [Herbaspirillum seropedicae SmR1] Length = 319 Score = 118 bits (296), Expect = 5e-28, Method: Compositional matrix adjust. Identities = 103/301 (34%), Positives = 153/301 (50%), Gaps = 10/301 (3%) Query: 16 WLRDLRTALPHAGIRVWQSGDT---AAADYAIVWKPPAEMLQGRPELKAVFNLGAGVDAI 72 W+ L+ A+P I + D A AIV P L+ P L V ++ AGV+ + Sbjct: 24 WISALQAAMPEERILPCEQLDADQLAQCRVAIVANPDPAQLKRMPNLLWVHSVWAGVERL 83 Query: 73 LKLNVVPDTLPLIRIDDGGMAVQVAEFATHAVLRYYRRLDQYERQARSNGGEWKP--LPP 130 + ++ L L R+ D +A +AE L +R + Y R+ R + E +P LP Sbjct: 84 VA-DLGQSDLQLTRLVDPRLAATMAEAVLAWTLYLHRDMPLYARRQREHHWEAQPYVLPE 142 Query: 131 FERSGFGIGVLGLGVLGQRICESLQHFGFEVSGWSRSPKTLPGVKAYAGSAQLNEFLRNA 190 R + +LGLG LG+ +L+ F V+GWSRS + GV+ + G A L + L Sbjct: 143 QRR----VSLLGLGALGEAAAAALRQARFAVNGWSRSAREAEGVQTFHGPAGLAQMLAQT 198 Query: 191 RVLICMLPLTPDTAGILNRDTLSQLPRGAYLINLARGGHLVEADLPPLLESGQIAGATLD 250 +L+C+LPLT +T G+++ L+ LP A LIN ARG + +A L L++GQ+ A LD Sbjct: 199 DILVCLLPLTAETRGLIDAAHLAMLPPQAQLINFARGPIIDDAALKAALDAGQLRHAVLD 258 Query: 251 VFQEEPLPPHHPFWKDPRIAITPHISALTVRSEAARQIAEKIAALQRGEAVVGLVDRNKG 310 VF EPLPP W P + + PH SA T R A+ +A I + + VD +G Sbjct: 259 VFAVEPLPPAAWPWSHPAVTVLPHCSAPTDRQTASAIVAANIRRYRHSGEIAANVDLRRG 318 Query: 311 Y 311 Y Sbjct: 319 Y 319 >gi|300313156|ref|YP_003777248.1| D-2-hydroxyacid dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 316 Score = 92.4 bits (228), Expect = 4e-20, Method: Compositional matrix adjust. Identities = 80/251 (31%), Positives = 119/251 (47%), Gaps = 29/251 (11%) Query: 47 KPPAEMLQGRPELKAVFNLGAGVDAI---------LKLNVVPDTLPLIRIDDGGMAVQVA 97 K E + P L+ V G G D + + + PD L DD VA Sbjct: 59 KVSREFMARFPALEIVSVFGVGYDGVDVPAARERGIHVTHTPDVLN----DD------VA 108 Query: 98 EFATHAVLRYYRRLDQYERQARSNGGEWKPLP-PF--ERSGFGIGVLGLGVLGQRICESL 154 + A +L R + + +R ARS GEWK P PF + SG +G++GLG +GQ I + Sbjct: 109 DLAMALMLATARNVVRADRFARS--GEWKKGPFPFTTKVSGARLGIVGLGRIGQAIAKRA 166 Query: 155 QHFGFEVSGWSRSPKTLPGVKAYAGSAQLNEFLRNARVLICMLPLTPDTAGILNRDTLSQ 214 F ++S +RS K +P Y + R L+ + P T ++N + L Sbjct: 167 AAFDMQISYHNRSRKDVP----YTYVDSITALAREVDFLVMITPGGAGTRALVNAEVLEA 222 Query: 215 LPRGAYLINLARGGHLVEADLPPLLESGQIAGATLDVFQEEPLPPHHPFWKDPRIAITPH 274 L +LIN+ARG + EA L L++G IAGA LDVF +EP P D + +TPH Sbjct: 223 LGPKGFLINVARGSVVDEAALIAALKTGVIAGAGLDVFADEPNVPAELAALD-NVVLTPH 281 Query: 275 ISALTVRSEAA 285 +++ T+ + A Sbjct: 282 MASGTLVTRTA 292 >gi|300310832|ref|YP_003774924.1| D-isomer specific 2-hydroxyacid dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 319 Score = 82.8 bits (203), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 75/259 (28%), Positives = 108/259 (41%), Gaps = 7/259 (2%) Query: 52 MLQGRPELKAVFNLGAGVDAILKLNVVPDTLPLIRIDDGGMAVQVAEFATHAVLRYYRR- 110 ML+ P+LK V + GVD L+ ++ G + A+ +L RR Sbjct: 60 MLEAAPQLKIVSTISVGVDN-FDLDYFRQRGLMLAHTPGVLTEATADTIFALILASARRV 118 Query: 111 --LDQYERQARSNGGEWKPLPPFERSGFGIGVLGLGVLGQRICESLQH-FGFEVSGWSRS 167 L +Y + R G + G +G++G+G +G + H FG + +R Sbjct: 119 VELAEYVKAGRWKGSIGEAQFGVNVHGKTLGLIGMGRIGSAVARRAHHGFGMPILYHNRR 178 Query: 168 PKTLPGVKAYAGSAQLNEFLRNARVLICMLPLTPDTAGILNRDTLSQLPRGAYLINLARG 227 P + A + L A + MLPLTP T + + + R A IN +RG Sbjct: 179 PDPEAERELGARYVSKEDLLAQADFVCVMLPLTPQTERMFGAPEFALMKRSAIFINASRG 238 Query: 228 GHLVEADLPPLLESGQIAGATLDVFQEEPLPPHHPFWKDPRIAITPHISALT--VRSEAA 285 + EA L L+ I GA LDVF+ EPLP P K P + PHI + T R A Sbjct: 239 RIVDEAALIAALQDKTIHGAGLDVFEVEPLPLQSPLLKLPNVVALPHIGSATHETRLAMA 298 Query: 286 RQIAEKIAALQRGEAVVGL 304 + A RGE V L Sbjct: 299 ELAVTNLIAGLRGEPVAHL 317 >gi|300310960|ref|YP_003775052.1| dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 326 Score = 79.3 bits (194), Expect = 4e-16, Method: Compositional matrix adjust. Identities = 72/245 (29%), Positives = 115/245 (46%), Gaps = 25/245 (10%) Query: 50 AEMLQGRPELKAVFNLGAG-----VDAILKLNVVPDTLPLIRIDDGGMAVQVAEFATHAV 104 A + P+LKAV N+ G V A + V+ P + + A+F + Sbjct: 59 AALFAANPQLKAVCNMAVGYNNIDVAAATQAGVMATNTPDV------LNETTADFGWALM 112 Query: 105 LRYYRRLDQYERQARSNGGEWK--PLPPF---ERSGFGIGVLGLGVLGQRICESLQHFGF 159 + RR+ + E R+ G+WK F + G +G++G+G +GQ I F Sbjct: 113 MAAARRITESEHFLRA--GKWKKWSYDSFVGPDIHGATLGIIGMGRIGQAIARRSLGFDM 170 Query: 160 EVSGWSRSPKTLPGVKAYAGSAQL---NEFLRNARVLICMLPLTPDTAGILNRDTLSQLP 216 +V +RS + P ++A A A+ E LR A +I +LP + DT + L+ + Sbjct: 171 QVLYHNRS-RLAPELEARANHARYVSKEELLRQADHVILVLPYSKDTHHTIAAAELALMK 229 Query: 217 RGAYLINLARGGHLVEADLPPLLESGQIAGATLDVFQEEP-LPPHHPFWKDPRIAITPHI 275 A L N+ARGG + +A L L G+IA A +DVF+ EP P F + +TPHI Sbjct: 230 PTATLTNIARGGIVDDAALIAALREGRIAAAGVDVFENEPAFKPE--FLDLSNVVLTPHI 287 Query: 276 SALTV 280 ++ + Sbjct: 288 ASAST 292 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.139 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,023,234 Number of extensions: 42191 Number of successful extensions: 160 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 151 Number of HSP's successfully gapped: 11 Length of query: 311 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 226 Effective length of database: 1,217,395 Effective search space: 275131270 Effective search space used: 275131270 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)