BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0137.p (227 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310406|ref|YP_003774498.1| glutathione S-transferase [Herb... 272 2e-74 gi|300312317|ref|YP_003776409.1| glutathione S-transferase [Herb... 75 5e-15 gi|300311872|ref|YP_003775964.1| glutathione S-transferase famil... 60 2e-10 >gi|300310406|ref|YP_003774498.1| glutathione S-transferase [Herbaspirillum seropedicae SmR1] Length = 206 Score = 272 bits (695), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 136/203 (66%), Positives = 159/203 (78%) Query: 25 MKLYYHPLSGHSHRARLFLSLLGAQHELVLVDLKKGEQKSPELLKLNRFGQVPVLQDGDT 84 MK+++HPLSGH+HRA LF SLLG ELV VDL G K P L LN FGQVPVL+DG+ Sbjct: 1 MKIHHHPLSGHAHRAVLFASLLGLPAELVEVDLAAGAHKKPAFLALNPFGQVPVLEDGEV 60 Query: 85 VVSDSNAILIYLAKSLGRSDWLPENPAAAAAVQRWLSVAAGEIARGPAAARLVTVFGAPI 144 V++DSNAIL+YLAK GRSDWLPE+ AAAVQRWLSVAAGE+A GPAAARLVTVFGA Sbjct: 61 VIADSNAILVYLAKKAGRSDWLPEDAQGAAAVQRWLSVAAGELAYGPAAARLVTVFGAAF 120 Query: 145 DADDAIRRAHAILTLVEQELATRTWLAADQATIADVALYSYISSAPEGNVDLSAYPHTRQ 204 +A++ I+RAH +L +EQ LA R WLAAD TIADVA+YSY++ APEGNVDL YP + Sbjct: 121 NAEEVIQRAHLLLGRLEQHLAKRDWLAADHPTIADVAVYSYLARAPEGNVDLRGYPGVQG 180 Query: 205 WLARIEALPGFVEFQKTAIGLAA 227 +L RIEALPGFV F +TA GL A Sbjct: 181 FLQRIEALPGFVPFAQTAAGLMA 203 >gi|300312317|ref|YP_003776409.1| glutathione S-transferase [Herbaspirillum seropedicae SmR1] Length = 230 Score = 74.7 bits (182), Expect = 5e-15, Method: Compositional matrix adjust. Identities = 50/189 (26%), Positives = 89/189 (47%), Gaps = 13/189 (6%) Query: 35 HSHRARLFLSLLGAQHELVLVDLKKGEQKSPELLKLNRFGQVPVLQDGD------TVVSD 88 + H+ + L G + +D+ G+Q +PE LK++ ++P + D D + + Sbjct: 11 NGHKVHIMLEECGLPYTAHAIDIGAGDQFTPEFLKISPNNKIPAIVDPDGPDGEPISLFE 70 Query: 89 SNAILIYLAKSLGRSDWLPENPAAAAAVQRWLSVAAGEIARGPAAARLVTVFGAPIDADD 148 S AILIYLA GR +L E V +W+ G + A ++ AP + Sbjct: 71 SGAILIYLAGKTGR--FLGETTREKYEVLQWVMFQMGGLGPMLGQAHHFRLY-APEQIEY 127 Query: 149 AIRR----AHAILTLVEQELATRTWLAADQATIADVALYSYISSAPEGNVDLSAYPHTRQ 204 A+ R A + +++++L +LA + TIAD+A + + S +DL +P+ ++ Sbjct: 128 AVNRYTNEARRLYQVMDKQLGQHAYLAGEHYTIADIACFPWTRSHKNQGIDLDDFPNVKR 187 Query: 205 WLARIEALP 213 W I A P Sbjct: 188 WYDAISARP 196 >gi|300311872|ref|YP_003775964.1| glutathione S-transferase family protein [Herbaspirillum seropedicae SmR1] Length = 235 Score = 59.7 bits (143), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 47/177 (26%), Positives = 84/177 (47%), Gaps = 18/177 (10%) Query: 25 MKLYYHPLSGHSHRARLFLSLLGAQHELVLVDLKKGEQKSPELLKLNRFGQVPVLQDGDT 84 ++LY P + + + + L G +E LV + +Q SPE L LN ++P + D + Sbjct: 22 IQLYSLP-TPNGVKVSIMLEETGLPYEAHLVSFETNDQMSPEFLSLNPNNKIPAIIDPNG 80 Query: 85 ------VVSDSNAILIYLAKSLGRSDWLPENPAAAAAVQRWLSVAAGEIARGPAAARLVT 138 + +S AIL+YLA+ G+ +LP + AA V +W+ G + GP +L Sbjct: 81 PGGQPLPLFESGAILLYLAEKTGQ--FLPADAAARYQVIQWVMFQMGGV--GPMFGQLGF 136 Query: 139 V-------FGAPIDADDAIRRAHAILTLVEQELATRTWLAADQATIADVALYSYISS 188 + D + + +L ++++ LA WL D +IAD+A + +I + Sbjct: 137 FHRFAGKEYEDKRPRDRYVNESKRLLGVMDKRLAQSQWLGGDAYSIADIATFPWIRN 193 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.133 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 658,728 Number of extensions: 23306 Number of successful extensions: 102 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 105 Number of HSP's successfully gapped: 3 Length of query: 227 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 145 Effective length of database: 1,231,600 Effective search space: 178582000 Effective search space used: 178582000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)