BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0135.p (227 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313278|ref|YP_003777370.1| vanillate O-demethylase (vanill... 70 2e-13 gi|300313415|ref|YP_003777507.1| phenylacetic acid degradation N... 56 2e-09 >gi|300313278|ref|YP_003777370.1| vanillate O-demethylase (vanillate degradation ferredoxin-like) oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 318 Score = 70.1 bits (170), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 62/192 (32%), Positives = 92/192 (47%), Gaps = 28/192 (14%) Query: 27 ARSKAELAFGEEIAALVNAAKGGARHVRVVSQPDDDVEVSGRIDI-ALLKQMLPFDDYDF 85 ARS A AF E IAA AA RVV DD E + ++D+ ALL Q P Sbjct: 140 ARSPARQAFRERIAASGFAA-------RVVHHYDDG-EAAQKLDLDALLAQADPATH--L 189 Query: 86 YLCGPAAFMQQMYDGLRAMNVIDARIHAEAFGPSGLQRQIDAQAEQVPLKPASADTVPVL 145 Y+CGPA F+ + D RA +A++H E FG + P+K A + + Sbjct: 190 YVCGPAGFIAHVVDTARARGWPEAQVHFEYFGAA-------------PVK-ADGNGAFEV 235 Query: 146 FMRSAKEARWTSDSGSLLELAEQRGLNPESSCRDGRCGTCRTRVLQGEVTY---VKTPEF 202 + S+ + +++++ + G++ SC G CGTC TRVL+GE + T E Sbjct: 236 KLASSGQVYTIPPERTVIQVLCEHGVDVPVSCEQGICGTCLTRVLEGEPDHRDQYLTDEE 295 Query: 203 AVADDEALICCA 214 A+D+ CC+ Sbjct: 296 RAANDQFTPCCS 307 >gi|300313415|ref|YP_003777507.1| phenylacetic acid degradation NADH oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 356 Score = 56.2 bits (134), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 54/205 (26%), Positives = 90/205 (43%), Gaps = 13/205 (6%) Query: 23 LFQSARSKAELAFGEEIAALVNAAKGGARHVRVVSQPDDDVEV-SGRID----IALLKQM 77 L + R+ + + F EE+ L +A V V+S+ DVE+ +GRID A Sbjct: 145 LVYANRASSSVIFKEELTDLKDAYLERFNVVYVMSREQQDVELFNGRIDRAKCDAFFASW 204 Query: 78 LPFDDYDF-YLCGPAAFMQQMYDGLRAMNVIDARIHAEAFGPSGLQRQIDAQAEQVPLKP 136 + D D +LCGP +Q + L+A + +I E F S R A A + + Sbjct: 205 IDLKDVDAAFLCGPEEMVQAVAASLQAHGLPKTQIKTELFAASTPPR---AHAARTVVGK 261 Query: 137 ASADTVPVLFMRSAKEARWTSDSGSLLELAEQRGLNPESSCRDGRCGTCRTRVLQGEV-- 194 + V V+ + S+LE + G++ SC+ G C TCR +V++G+V Sbjct: 262 QECE-VTVIVDGYHNVFTMDKEKESVLEAGLKHGIDLRYSCKGGVCATCRCKVVEGKVDM 320 Query: 195 -TYVKTPEFAVADDEALICCALPAA 218 ++ +A L C + P + Sbjct: 321 DANYALEDYEIARGFVLSCQSFPVS 345 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.135 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 689,748 Number of extensions: 26484 Number of successful extensions: 79 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 78 Number of HSP's successfully gapped: 2 Length of query: 227 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 145 Effective length of database: 1,231,600 Effective search space: 178582000 Effective search space used: 178582000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)