BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0126.p (590 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311162|ref|YP_003775254.1| amidase family protein [Herbasp... 142 1e-34 gi|300311728|ref|YP_003775820.1| indole acetamide hydrolase [Her... 104 2e-23 gi|300310745|ref|YP_003774837.1| amidase family protein [Herbasp... 99 8e-22 gi|300310674|ref|YP_003774766.1| amidase family protein [Herbasp... 90 5e-19 gi|300313752|ref|YP_003777844.1| Asp-tRNAAsn/Glu-tRNAGln amidotr... 83 7e-17 >gi|300311162|ref|YP_003775254.1| amidase family protein [Herbaspirillum seropedicae SmR1] Length = 450 Score = 142 bits (357), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 124/383 (32%), Positives = 190/383 (49%), Gaps = 27/383 (7%) Query: 216 PLSGLRFAVKDIFDVAGYPTGCGNPHMLALSGIKTASAPSVIA--LAQAGATFIGKTYTD 273 PL G+ +VKD+FDVAG T G+ + L G ASA + + L +AGA IGKT Sbjct: 68 PLEGVPVSVKDLFDVAGETTLAGS---VVLRGKPVASAHAAVVQNLLKAGAILIGKTNMT 124 Query: 274 ELAFSMNGKNAHFGTPRN-------GGAPERIPGGSSSGSASAVSNGLADVALGTDTGGS 326 E A+S G N H+GTP+N GG RIPGGSSSG+A +V++G+A A+G+DTGGS Sbjct: 125 EFAYSGLGINPHYGTPQNAWERNIEGG---RIPGGSSSGAAISVTDGMAFAAVGSDTGGS 181 Query: 327 VRAPASHCGLIGLRPTHARVSLKGCMDLAPSFDTCGWFARDIDSFARVGEVLLRED-TCL 385 VR P++ GL G +PT ARVS++G + L+ D+ G A + A + VL ED + Sbjct: 182 VRIPSALNGLTGFKPTAARVSMQGVLPLSAYLDSIGPLAVSVQCCAIMDAVLAGEDYVAV 241 Query: 386 LPDGMPAMPQVLVAADVLALLEPRVQAVFMQTLERLSGLIGAPLPVKTATPSFDALYWAF 445 L + + + VL ++ V + L +L+ + V+ P+F+AL A Sbjct: 242 LAHPLRGLRLLAPTNVVLDGMDDTVGQAYRTALGKLA--AAGAMIVEAEVPAFNALA-AI 298 Query: 446 RHIQGY---EAWQSHGDTITRHDLQLGPGVAERFAWSAKITPQQMEEHSNVRQRFTEDFL 502 G+ EA+ H I + V R ++ + + + R R+ Sbjct: 299 NAKGGFTAAEAYAWHRALIAENAAGYDQRVVSRILRGKDMSAADLLDVLHARPRWIAQVE 358 Query: 503 RLLGNDRVIVLPSMPDVAPLLSDPEEVLENYRNQAVRML----CLAGLSGCPQISLPLMT 558 L +V+P++P VAP +++ + + Y +ML + L GC +SLP Sbjct: 359 AQLAGYDALVMPTVPIVAPRIAELQASDDAYYGANGKMLRNPTLINFLDGC-ALSLPCHA 417 Query: 559 LDGAPFGFSIIGPQGSDQALIRL 581 AP G S+ G D+ L+ + Sbjct: 418 PGSAPVGLSLAACGGQDKRLLSI 440 >gi|300311728|ref|YP_003775820.1| indole acetamide hydrolase [Herbaspirillum seropedicae SmR1] Length = 468 Score = 104 bits (259), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 125/402 (31%), Positives = 175/402 (43%), Gaps = 43/402 (10%) Query: 215 GPLSGLRFAVKDIFDVAGYPTGCGNPHMLALSGIKTASAPSVIALAQAGATFIGKTYTDE 274 GPLSG+ A+KD DVAG + G + + + A V L AGA GK E Sbjct: 64 GPLSGIPIAIKDNIDVAGIASTAGTAALR--NNVPQRHATVVEYLFGAGALHAGKANMHE 121 Query: 275 LAFSMNGKNAHFGTPRNGGAPERIPGGSSSGSASAVSNGLADVALGTDTGGSVRAPASHC 334 LAF + N FG RN E I GGSS G + ++ LA A+GTDTGGSVR PA+ C Sbjct: 122 LAFGITNNNGVFGPCRNPYDAELIAGGSSGGCGALLAARLAPAAVGTDTGGSVRIPAALC 181 Query: 335 GLIGLRPTHARVSLKGCMDLAPSFDTCGWFARDIDSFARVGEVLL--REDTCLLPDGMPA 392 G++GLRPT R S G + ++ + DT G AR + A + +++ R+ LL A Sbjct: 182 GVVGLRPTSRRYSQHGVVPISHTRDTPGPMARSVADVALLDQIMAGSRDSAPLL-----A 236 Query: 393 MPQVLVAADVL---------ALLEPRVQAVFMQTLERLSGLIGAPLPVKTATPSFDA--- 440 +P + VL +E A MQ E L+ LP T P A Sbjct: 237 LPPSQLRLGVLRNPSWQGLEEEVEANANAALMQLREAGVELVDVDLP--TLAPLSHAAGF 294 Query: 441 ---LYWAFRHIQGYEAWQSHGDTITRHDLQLG-PGVAERFAWSAKITPQQMEEHS----- 491 L+ + Y D I R ++G P VA + Q + EH+ Sbjct: 295 AIVLHEFLIDLPAYLQASDARDVIKRVLAEVGSPDVARIIQATLD---QPVPEHAYRLAL 351 Query: 492 ---NVRQRFTEDFLRLLGNDRVIVLPSMPDVAPLLSDPEEVLENYRNQA----VRMLCLA 544 Q D R G D +I + PL D L R R A Sbjct: 352 EQRRTMQALYRDCFRSQGVDALIFPTTALTARPLGEDDTVALRGERVPTFPAFARNADGA 411 Query: 545 GLSGCPQISLPL-MTLDGAPFGFSIIGPQGSDQALIRLSRKL 585 ++G P IS+P+ ++ G P G + GP+GSD+ L++++ L Sbjct: 412 SIAGLPSISIPVGLSEYGLPVGLELDGPEGSDRRLLQVAASL 453 >gi|300310745|ref|YP_003774837.1| amidase family protein [Herbaspirillum seropedicae SmR1] Length = 611 Score = 99.4 bits (246), Expect = 8e-22, Method: Compositional matrix adjust. Identities = 123/411 (29%), Positives = 185/411 (45%), Gaps = 30/411 (7%) Query: 196 QAAHCFVPYPASMTTAISSGPLSGLRFAVKDIFDVAGYPTGCGNPHMLALSGIKTASAPS 255 QAA + + T + S PL G+ FAVKD DVAG PT P S + SA Sbjct: 62 QAAALDLMWQIKGETVLESMPLFGIPFAVKDNIDVAGMPTTAACPE---FSRVAETSATV 118 Query: 256 VIALAQAGATFIGKTYTDELAFSMNGKNAHFGTPRNGGAPERIPGGSSSGSASAVSNGLA 315 V L AGA +GKT D+ A + G + +G RN AP+ + GGSSSGSA AV+ G Sbjct: 119 VQRLQAAGALLVGKTNLDQFATGLVGVRSPYGAVRNAIAPDYVSGGSSSGSAVAVALGQV 178 Query: 316 DVALGTDTGGSVRAPASHCGLIGLRPTHARVSLKGCMDLAPSFDTCGWFARDI-DSFARV 374 ALGTDT GS R PA+ L+GL+P+ +S +G + + D FA D+ D++ + Sbjct: 179 MFALGTDTAGSGRIPAAFNHLVGLKPSRGLISAQGLVPACRTLDCISIFAHDVADAWHVL 238 Query: 375 GEVLLREDTCLLPDGMPAM------PQVLVAADVLALLEPRVQAVFMQTLERLSGLIGAP 428 + +P + ++ + A + Q + +TL R++ L G Sbjct: 239 HSAAGYDAADAYARKLPMLGLKRRGYRIAIPAATEFYGDLAAQDAWDRTLARIAELPGVT 298 Query: 429 LPVKTATPSFDALYWAFRHIQGYEAWQSHGDTITRHDLQLGPGVAERFAWSA---KITPQ 485 L P DA ++ E + GD + + P V E A +A + Sbjct: 299 LARIPFAPFADAARLLYQGPWVAERRAALGDFFVKQPQAVHPVVREITAQAADYDAVAAF 358 Query: 486 QMEEHSNVRQRFTEDFLRLLGNDRVIVLPS--MPDVAPLLSDPEEVLEN----YRNQAVR 539 Q + +R E L G D ++V + MP +A + +DP V N Y V Sbjct: 359 QGQYRLAELRRQAEQLLE--GADLMLVPTTTGMPTIAEVQADP--VRRNSQLGYYTNFVN 414 Query: 540 MLCLAGLSGCPQISLP-LMTLDGAPFGFSIIGPQGSDQALIRLSRKLIDEL 589 ++ ++ L ++P L DG P G ++IGP G+D L +L +L Sbjct: 415 LMDMSAL------AIPGLWRNDGLPAGVTLIGPAGADHLLAEAGARLQHQL 459 >gi|300310674|ref|YP_003774766.1| amidase family protein [Herbaspirillum seropedicae SmR1] Length = 388 Score = 90.1 bits (222), Expect = 5e-19, Method: Compositional matrix adjust. Identities = 69/152 (45%), Positives = 87/152 (57%), Gaps = 1/152 (0%) Query: 218 SGLRFAVKDIFDVAGYPTGCGNPHMLALSGIKTASAPSVIALAQAGATFIGKTYTDELAF 277 SG A+KD D+AG PT CG LA + +A A V L +AG + ELA+ Sbjct: 25 SGPTVAIKDCIDIAGQPT-CGGSAALADAPPASAHADVVARLLEAGWQITARANMHELAY 83 Query: 278 SMNGKNAHFGTPRNGGAPERIPGGSSSGSASAVSNGLADVALGTDTGGSVRAPASHCGLI 337 M G N GT N P + GGSSSGSASAV GL DV++G+DTGGS+R PA+ CG+I Sbjct: 84 GMTGINEWNGTAVNPQDPACMTGGSSSGSASAVGAGLVDVSIGSDTGGSIRLPAACCGVI 143 Query: 338 GLRPTHARVSLKGCMDLAPSFDTCGWFARDID 369 G +P+ RVS G S D G F+R +D Sbjct: 144 GFKPSFGRVSRAGAYPALSSLDCLGPFSRSMD 175 >gi|300313752|ref|YP_003777844.1| Asp-tRNAAsn/Glu-tRNAGln amidotransferase subunit A [Herbaspirillum seropedicae SmR1] Length = 471 Score = 82.8 bits (203), Expect = 7e-17, Method: Compositional matrix adjust. Identities = 61/151 (40%), Positives = 82/151 (54%), Gaps = 3/151 (1%) Query: 214 SGPLSGLRFAVKDIFDVAGYPTGCGNPHMLALSGIKTASAPSVIALAQAGATFIGKTYTD 273 S PL G+ F++KD D+AG PT P A S + SA V L GA +GK D Sbjct: 67 SLPLYGVPFSIKDNIDLAGVPTTAACP---AFSYVPRESATLVAQLIALGAIPVGKCNLD 123 Query: 274 ELAFSMNGKNAHFGTPRNGGAPERIPGGSSSGSASAVSNGLADVALGTDTGGSVRAPASH 333 + A +NG + +G RN P+ GGSSSGS+ AV+ GL+ ALGTDT GS R PA+ Sbjct: 124 QFATGLNGTRSPYGACRNSVHPDYPSGGSSSGSSLAVALGLSSFALGTDTAGSGRVPAAL 183 Query: 334 CGLIGLRPTHARVSLKGCMDLAPSFDTCGWF 364 L+GL+ T +S G + + D +F Sbjct: 184 NNLVGLKATKGLLSTAGVVPACRTLDCVTFF 214 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,851,236 Number of extensions: 76452 Number of successful extensions: 248 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 234 Number of HSP's successfully gapped: 11 Length of query: 590 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 499 Effective length of database: 1,188,985 Effective search space: 593303515 Effective search space used: 593303515 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)