BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0117.p (483 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313752|ref|YP_003777844.1| Asp-tRNAAsn/Glu-tRNAGln amidotr... 287 1e-78 gi|300310745|ref|YP_003774837.1| amidase family protein [Herbasp... 285 5e-78 gi|300311728|ref|YP_003775820.1| indole acetamide hydrolase [Her... 126 3e-30 gi|300311162|ref|YP_003775254.1| amidase family protein [Herbasp... 120 2e-28 gi|300309904|ref|YP_003773996.1| aspartate tRNAAsn/Glu-tRNAGln a... 119 7e-28 >gi|300313752|ref|YP_003777844.1| Asp-tRNAAsn/Glu-tRNAGln amidotransferase subunit A [Herbaspirillum seropedicae SmR1] Length = 471 Score = 287 bits (734), Expect = 1e-78, Method: Compositional matrix adjust. Identities = 187/466 (40%), Positives = 269/466 (57%), Gaps = 28/466 (6%) Query: 16 SFDF--PTLSAAYRDG-LSPLALVEEVHRRIRE-----HRDRHIWTYLIPQEQLIEQTRK 67 SFD L+AAYR+G L P V +R+RE + ++ +L+ Q + + Sbjct: 4 SFDLRIDALTAAYRNGSLDP----RTVMQRLRESAAALNPTYRLYIHLLSQAETEPYLQA 59 Query: 68 LEQAAAHGASLPLFGLPFGVKDNIDVAGLPTGAACASFTYTATRTAHVVERLQAAGAILI 127 LEQ + H SLPL+G+PF +KDNID+AG+PT AAC +F+Y +A +V +L A GAI + Sbjct: 60 LEQQSPH--SLPLYGVPFSIKDNIDLAGVPTTAACPAFSYVPRESATLVAQLIALGAIPV 117 Query: 128 GKQNMDQFATGLVGIRSPQGYCRNPFNPDYVPGGSSSGSAVAVSTGQVSFSIGSDTGGSG 187 GK N+DQFATGL G RSP G CRN +PDY GGSSSGS++AV+ G SF++G+DT GSG Sbjct: 118 GKCNLDQFATGLNGTRSPYGACRNSVHPDYPSGGSSSGSSLAVALGLSSFALGTDTAGSG 177 Query: 188 RVPAALNNIVGLKPTPGRVSSYGFLYCNRSFDVPPVFALTSEDA---YAVLDQIEGYDAR 244 RVPAALNN+VGLK T G +S+ G + R+ D F T+ +A A+ Q D R Sbjct: 178 RVPAALNNLVGLKATKGLLSTAGVVPACRTLDCVTFFTATAAEASHLLALCTQSGQPDER 237 Query: 245 DIYS-VNVPRE-----EPPARLPKHFRFGLPAEKYLNFFGDQLAEAQFETSVRRLIDLGG 298 D YS N+P +PP P+ RFG+P + L F G + + F ++ ++ +GG Sbjct: 238 DEYSRANLPHNDASAFQPP---PQGLRFGVP--RQLEFLGCSESPSLFVEALAQMEAIGG 292 Query: 299 EAMEIDFEPFAEAGKLVFNSAFIAERWLTYGAAAEPADPHVHPAVRLAILAAKRYSAADG 358 A+ IDF PF A +L++ ++AER+ T A + + P +R + A +YSA D Sbjct: 293 VAVPIDFSPFLAAARLLYEGPWVAERYSTIEALLQERPDDILPVIRDVLRKAPQYSAVDT 352 Query: 359 FDAVYALESHKRKAAQELDKVDILALPTAGTVYRIKDVEADPVALNANLGYYTYFANPLR 418 F A Y L+ K + + L VD + P ++++ PV N++LG+YT F N L Sbjct: 353 FRAQYRLQQLKAECDRILATVDCVVTPAYPRPVTLEELREQPVQRNSDLGWYTNFMNMLD 412 Query: 419 LAAISVPAGLRSDGLPFGLCLLARSFEDKRLLPYAQAFHDAVGGKL 464 AA++ P +GLP+G+ + R+F D+ LL A A A G L Sbjct: 413 YAAVATPMNFMKNGLPWGITVFGRAFSDQALLGVADALQRAHGRPL 458 >gi|300310745|ref|YP_003774837.1| amidase family protein [Herbaspirillum seropedicae SmR1] Length = 611 Score = 285 bits (729), Expect = 5e-78, Method: Compositional matrix adjust. Identities = 189/453 (41%), Positives = 262/453 (57%), Gaps = 26/453 (5%) Query: 24 AAYRDGLSP--------LALVEEVHRRIREHRDRHIWTYLIPQEQLIEQTRKLE-----Q 70 AAYR+G S LAL+E+ R +W +P +QL +Q L+ + Sbjct: 21 AAYRNGSSTPGKTVASLLALIEQADRE-------EVWISRVPAKQLKQQAAALDLMWQIK 73 Query: 71 AAAHGASLPLFGLPFGVKDNIDVAGLPTGAACASFTYTATRTAHVVERLQAAGAILIGKQ 130 S+PLFG+PF VKDNIDVAG+PT AAC F+ A +A VV+RLQAAGA+L+GK Sbjct: 74 GETVLESMPLFGIPFAVKDNIDVAGMPTTAACPEFSRVAETSATVVQRLQAAGALLVGKT 133 Query: 131 NMDQFATGLVGIRSPQGYCRNPFNPDYVPGGSSSGSAVAVSTGQVSFSIGSDTGGSGRVP 190 N+DQFATGLVG+RSP G RN PDYV GGSSSGSAVAV+ GQV F++G+DT GSGR+P Sbjct: 134 NLDQFATGLVGVRSPYGAVRNAIAPDYVSGGSSSGSAVAVALGQVMFALGTDTAGSGRIP 193 Query: 191 AALNNIVGLKPTPGRVSSYGFLYCNRSFDVPPVFALTSEDAYAVLDQIEGYDARDIYSVN 250 AA N++VGLKP+ G +S+ G + R+ D +FA DA+ VL GYDA D Y+ Sbjct: 194 AAFNHLVGLKPSRGLISAQGLVPACRTLDCISIFAHDVADAWHVLHSAAGYDAADAYARK 253 Query: 251 VPREEPPARLPKHFRFGLPAEKYLNFFGDQLAEAQFETSVRRLIDLGGEAM-EIDFEPFA 309 +P R +R +PA F+GD A+ ++ ++ R+ +L G + I F PFA Sbjct: 254 LPMLGLKRR---GYRIAIPAAT--EFYGDLAAQDAWDRTLARIAELPGVTLARIPFAPFA 308 Query: 310 EAGKLVFNSAFIAERWLTYGAAAEPADPHVHPAVRLAILAAKRYSAADGFDAVYALESHK 369 +A +L++ ++AER G VHP VR A Y A F Y L + Sbjct: 309 DAARLLYQGPWVAERRAALGDFFVKQPQAVHPVVREITAQAADYDAVAAFQGQYRLAELR 368 Query: 370 RKAAQELDKVDILALPTAGTVYRIKDVEADPVALNANLGYYTYFANPLRLAAISVPAGLR 429 R+A Q L+ D++ +PT + I +V+ADPV N+ LGYYT F N + ++A+++P R Sbjct: 369 RQAEQLLEGADLMLVPTTTGMPTIAEVQADPVRRNSQLGYYTNFVNLMDMSALAIPGLWR 428 Query: 430 SDGLPFGLCLLARSFEDKRLLPYAQAFHDAVGG 462 +DGLP G+ L+ + D L +GG Sbjct: 429 NDGLPAGVTLIGPAGADHLLAEAGARLQHQLGG 461 >gi|300311728|ref|YP_003775820.1| indole acetamide hydrolase [Herbaspirillum seropedicae SmR1] Length = 468 Score = 126 bits (317), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 133/428 (31%), Positives = 190/428 (44%), Gaps = 50/428 (11%) Query: 59 EQLIEQTRKLEQAAAHGASLPLFGLPFGVKDNIDVAGLPTGAACASFTYTA-TRTAHVVE 117 EQL Q R L++ HG PL G+P +KDNIDVAG+ + A A+ R A VVE Sbjct: 48 EQLRSQARMLDEQGGHG---PLSGIPIAIKDNIDVAGIASTAGTAALRNNVPQRHATVVE 104 Query: 118 RLQAAGAILIGKQNMDQFATGLVGIRSPQGYCRNPFNPDYVPGGSSSGSAVAVSTGQVSF 177 L AGA+ GK NM + A G+ G CRNP++ + + GGSS G ++ Sbjct: 105 YLFGAGALHAGKANMHELAFGITNNNGVFGPCRNPYDAELIAGGSSGGCGALLAARLAPA 164 Query: 178 SIGSDTGGSGRVPAALNNIVGLKPTPGRVSSYGFLYCNRSFDVPPVFALTSEDAYAVLDQ 237 ++G+DTGGS R+PAAL +VGL+PT R S +G + + + D P A + D A+LDQ Sbjct: 165 AVGTDTGGSVRIPAALCGVVGLRPTSRRYSQHGVVPISHTRDTPGPMARSVADV-ALLDQ 223 Query: 238 IEGYDARDIYSVNVPREEPPARLPKHFRFGLPAEKYLNFFGDQLAEAQFETSVRRLIDLG 297 I +RD + P P P R G+ +++ EA ++ +L + G Sbjct: 224 IMA-GSRD----SAPLLALP---PSQLRLGVLRNPSWQGLEEEV-EANANAALMQLREAG 274 Query: 298 GEAMEIDFEPFA----EAGKLVFNSAFIAE----------RWLTYGAAAEPADPHVHPAV 343 E +++D A AG + F+ + R + AE P V + Sbjct: 275 VELVDVDLPTLAPLSHAAGFAIVLHEFLIDLPAYLQASDARDVIKRVLAEVGSPDVARII 334 Query: 344 RLAILAAKRYSAADGFDAVYALESHKRKAAQELDK-------VDILALPTAGTVYRIKDV 396 + + A Y L +R+ Q L + VD L PT R Sbjct: 335 QATLDQPVPEHA-------YRLALEQRRTMQALYRDCFRSQGVDALIFPTTALTARPLG- 386 Query: 397 EADPVALNAN-LGYYTYFANPLR------LAAISVPAGLRSDGLPFGLCLLARSFEDKRL 449 E D VAL + + FA L +IS+P GL GLP GL L D+RL Sbjct: 387 EDDTVALRGERVPTFPAFARNADGASIAGLPSISIPVGLSEYGLPVGLELDGPEGSDRRL 446 Query: 450 LPYAQAFH 457 L A + Sbjct: 447 LQVAASLE 454 >gi|300311162|ref|YP_003775254.1| amidase family protein [Herbaspirillum seropedicae SmR1] Length = 450 Score = 120 bits (301), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 125/391 (31%), Positives = 174/391 (44%), Gaps = 25/391 (6%) Query: 79 PLFGLPFGVKDNIDVAGLPTGAACASFTYTATRTAH--VVERLQAAGAILIGKQNMDQFA 136 PL G+P VKD DVAG T A +AH VV+ L AGAILIGK NM +FA Sbjct: 68 PLEGVPVSVKDLFDVAGETTLAGSVVLRGKPVASAHAAVVQNLLKAGAILIGKTNMTEFA 127 Query: 137 TGLVGIR----SPQGYCRNPFNPDYVPGGSSSGSAVAVSTGQVSFSIGSDTGGSGRVPAA 192 +GI +PQ +PGGSSSG+A++V+ G ++GSDTGGS R+P+A Sbjct: 128 YSGLGINPHYGTPQNAWERNIEGGRIPGGSSSGAAISVTDGMAFAAVGSDTGGSVRIPSA 187 Query: 193 LNNIVGLKPTPGRVSSYGFLYCNRSFDVPPVFALTSEDAYAVLDQIEGYDARDIYSVNVP 252 LN + G KPT RVS G L + D A++ + A++D + D +V Sbjct: 188 LNGLTGFKPTAARVSMQGVLPLSAYLDSIGPLAVSVQ-CCAIMDAV--LAGEDYVAV--- 241 Query: 253 REEPPARLPKHFRFGLPAEKYLNFFGDQLAEAQFETSVRRLIDLGGEAMEIDFEPFAEAG 312 A + R P L+ D + +A + T++ +L G +E + F Sbjct: 242 ----LAHPLRGLRLLAPTNVVLDGMDDTVGQA-YRTALGKLAAAGAMIVEAEVPAFNALA 296 Query: 313 KLVFNSAFIAERWLTYGAA--AEPADPHVHPAVRLAILAAKRYSAADGFDAVYALESHKR 370 + F A + A AE A + V IL K SAAD D ++A Sbjct: 297 AINAKGGFTAAEAYAWHRALIAENAAGYDQRVVS-RILRGKDMSAADLLDVLHARPRWIA 355 Query: 371 KAAQELDKVDILALPTAGTVY-RIKDVEADPVAL---NANLGYYTYFANPLRLAAISVPA 426 + +L D L +PT V RI +++A A N + N L A+S+P Sbjct: 356 QVEAQLAGYDALVMPTVPIVAPRIAELQASDDAYYGANGKMLRNPTLINFLDGCALSLPC 415 Query: 427 GLRSDGLPFGLCLLARSFEDKRLLPYAQAFH 457 P GL L A +DKRLL A Sbjct: 416 HAPGSA-PVGLSLAACGGQDKRLLSIGLAVE 445 >gi|300309904|ref|YP_003773996.1| aspartate tRNAAsn/Glu-tRNAGln amidotransferase subunit A-like protein [Herbaspirillum seropedicae SmR1] Length = 651 Score = 119 bits (297), Expect = 7e-28, Method: Compositional matrix adjust. Identities = 134/464 (28%), Positives = 198/464 (42%), Gaps = 69/464 (14%) Query: 59 EQLIEQTRKLEQAAAHGASLPLFGLPFGVKDNIDVAGLPT-GAACASFTYTATRTAHVVE 117 E+ + R+ Q+ G PL G+P VKDN D +PT G A T A A V+ Sbjct: 101 EEADQLDRERRQSGPRG---PLHGIPILVKDNYDTVDMPTTGGTLALATLQAQADAFQVK 157 Query: 118 RLQAAGAILIGKQNMDQFATGLVGIRSPQGYCRNPFNPDYVPGGSSSGSAVAVSTGQVSF 177 RL+ AGA+++GK M + A G+ + S G+ RNP++P PGGSS G+ AV+ + Sbjct: 158 RLREAGAVILGKTTMHELAAGVTTVSSLTGFTRNPYDPRRAPGGSSGGTGAAVAASFAAA 217 Query: 178 SIGSDTGGSGRVPAALNNIVGLKPTPGRVSSYGFLYCNRSFDVPPVFALTSEDAYAVLDQ 237 +GSDT GS R+PAA N+ GL+ T G S G + + + DV A + ED +LD Sbjct: 218 GMGSDTCGSIRIPAAHQNLFGLRTTRGLASRSGVMPLSSTQDVAAPLARSVEDLAIMLDA 277 Query: 238 IEGYDARDIYSVNVPREEPPARLPKHFRFGLPAEKYLN--------FFG----DQLAEAQ 285 G D +D +V+ +PK +R GL A+ FG D A Sbjct: 278 TVGSDPQDSSTVDA-----NGHIPKSYRDGLRADSLQGARIGVLRALFGAAPEDAEVSAA 332 Query: 286 FETSVRRLIDLGGEAMEIDFEPFAEAGKLVFNSAFIA-ERWLTYGAAAEPADPHVHPAVR 344 ++++L D G ++ E L+ S+ I E GA + HP Sbjct: 333 INKALQQLKDQGAIVTDVT---IPELDGLLSGSSIIPYEFKYDLGAYLQS-----HPGAP 384 Query: 345 LAILAAKRYSAAD------GFDAVYALESHKRKAAQELDKV------------------- 379 + L + G +++ K +EL+KV Sbjct: 385 VGSLGEILARGMEHELLEAGLRLRNSVDLQNPKDKEELEKVMLKRSALKSLMTDVMQKNH 444 Query: 380 -DILALPTAGTVYRIKDVEADPV--ALNANLGYYTYFANPLRLAAISVPAGLRSDGLPFG 436 D L P T+ R + +P A+N L T L A+++P G DGLP Sbjct: 445 LDTLVYP---TIQRKAALIGEPQGGAMNCQLSATTG------LPALALPVGFTEDGLPVS 495 Query: 437 LCLLARSFEDKRLLPYAQAFHDAVGGKLG--ATGHAFHGAAPGI 478 L LLA F + LL A + +G + +T G APG+ Sbjct: 496 LELLAPEFAEPALLGLAYGWEQKIGPRRSPYSTPALVAGKAPGL 539 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.137 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,574,792 Number of extensions: 66935 Number of successful extensions: 216 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 202 Number of HSP's successfully gapped: 11 Length of query: 483 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 394 Effective length of database: 1,198,455 Effective search space: 472191270 Effective search space used: 472191270 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)