BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0084.p (420 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310890|ref|YP_003774982.1| cysteine desulfurase [Herbaspir... 539 e-154 gi|300312444|ref|YP_003776536.1| cysteine desulfurase [Herbaspir... 454 e-129 gi|300312137|ref|YP_003776229.1| cysteine desulfurase NifS [Herb... 451 e-128 gi|300312171|ref|YP_003776263.1| cysteine desulfurase 2 protein ... 194 9e-51 gi|300311647|ref|YP_003775739.1| selenocysteine lyase [Herbaspir... 93 4e-20 >gi|300310890|ref|YP_003774982.1| cysteine desulfurase [Herbaspirillum seropedicae SmR1] Length = 408 Score = 539 bits (1388), Expect = e-154, Method: Compositional matrix adjust. Identities = 271/363 (74%), Positives = 295/363 (81%), Gaps = 7/363 (1%) Query: 1 MTASSACIPITSSGQDQTPLQFPIYLDYSATTPVDPLVATRMFAYLTEKFGNPASSSHSF 60 M A + C P L+FPIYLDY ATTPVDP VA M +LT+KFGN ASSSH F Sbjct: 1 MNAPAPCRPPAD-------LRFPIYLDYGATTPVDPQVARVMCDFLTDKFGNAASSSHPF 53 Query: 61 GWEAKAAVEKARKQVAALVGAQASEIVWTSGATESNNLALKSAAHALRAAGRGKHLITVA 120 GWEA+ AVE+AR QVA LVGAQ EIVWTSGATESNNLALK AA+ALRA GRG HLITV Sbjct: 54 GWEARRAVEQARAQVAGLVGAQPGEIVWTSGATESNNLALKGAAYALRAQGRGNHLITVP 113 Query: 121 TEHKAVLDTMHTLERAGFEVSFLQPGTDGLITVAQFAEALRPDTILASVMMVNNEIGVIQ 180 TEHKAVLDTMHTLE+AGFEV++LQPG DGLIT Q ALRPDT+LASVM VNNEIGVIQ Sbjct: 114 TEHKAVLDTMHTLEKAGFEVTYLQPGADGLITPQQLRAALRPDTLLASVMYVNNEIGVIQ 173 Query: 181 PIEELGEICAARDILFHVDAVQAVGKIAIDLATLKVDLMSFSAHKIYGPKGIGALFVRNN 240 PI ELGEICAA D++FHVDAVQA GKIAIDL LKVDLMSFSAHK+YGPKGIGAL+VR+N Sbjct: 174 PIAELGEICAAHDVVFHVDAVQAAGKIAIDLTRLKVDLMSFSAHKVYGPKGIGALYVRHN 233 Query: 241 PALRLEAQIDGGGHENGMRSGTLPTHQIVGMGEAFALAANLQDTEAARIGVLRDRLWEGL 300 P L+LEAQIDGGGHENGMRSGTL THQIVGMGEAFALA L + E+ RIG LRDRLW GL Sbjct: 234 PQLKLEAQIDGGGHENGMRSGTLATHQIVGMGEAFALAGRLLEEESRRIGALRDRLWAGL 293 Query: 301 KDLPGVVLNGSATARIPHNLNVSFDLDGEISVAGQLLDIAVSAGSACNSANAAPSYVLTA 360 DLPGV LNG A R+PHNLNVSF ++ QLL IAVSAGSACNSA AAPS+VL A Sbjct: 294 ADLPGVQLNGCARQRVPHNLNVSFPPTRHGTLGSQLLGIAVSAGSACNSAAAAPSFVLLA 353 Query: 361 LGR 363 +GR Sbjct: 354 IGR 356 >gi|300312444|ref|YP_003776536.1| cysteine desulfurase [Herbaspirillum seropedicae SmR1] Length = 417 Score = 454 bits (1169), Expect = e-129, Method: Compositional matrix adjust. Identities = 221/344 (64%), Positives = 267/344 (77%), Gaps = 4/344 (1%) Query: 21 QFPIYLDYSATTPVDPLVATRMFAYLTEKFGNPASSSHSFGWEAKAAVEKARKQVAALVG 80 FPIY+DYSATTPVDP VA +M YL E+FGNPAS SH +GW A+ AVE+AR++VA LV Sbjct: 17 HFPIYMDYSATTPVDPRVADKMIPYLREQFGNPASRSHMYGWTAEKAVEEAREEVAKLVN 76 Query: 81 AQASEIVWTSGATESNNLALKSAAHALRAAGRGKHLITVATEHKAVLDTMHTLERAGFEV 140 A + EI+WTSGATESNNLA+K AA ++ +GKH+ITV TEHKAVLDT LER GFE Sbjct: 77 ADSREIIWTSGATESNNLAIKGAAQFYKS--KGKHIITVKTEHKAVLDTFRELERIGFEA 134 Query: 141 SFLQPGTDGLITVAQFAEALRPDTILASVMMVNNEIGVIQPIEELGEICAARDILFHVDA 200 ++LQP +GL+TV Q A A+RPDTIL SVM+VNNEIGVIQP+ E+GE C ++ I+FH DA Sbjct: 135 TYLQPQDNGLVTVEQIAAAVRPDTILVSVMLVNNEIGVIQPVPEIGEFCRSKGIIFHSDA 194 Query: 201 VQAVGKIAIDLATLKVDLMSFSAHKIYGPKGIGALFVRNNPALRLEAQIDGGGHENGMRS 260 QA GK+ IDL KVDLMSFSAHK YGPKGIGAL+VR P +R+EAQ+ GGGHE G+RS Sbjct: 195 AQATGKVKIDLENWKVDLMSFSAHKSYGPKGIGALYVRRKPRVRIEAQMHGGGHERGLRS 254 Query: 261 GTLPTHQIVGMGEAFALAANLQDTEAARIGVLRDRLWEGLKDLPGVVLNGSATARIPHNL 320 GTL THQIVGMGEAF LA D+E ARI LRDRL +GL+++ +NG R+PHNL Sbjct: 255 GTLATHQIVGMGEAFRLAREEMDSELARIRALRDRLAKGLQEIEETYINGDMEHRVPHNL 314 Query: 321 NVSFD-LDGEISVAGQLLDIAVSAGSACNSANAAPSYVLTALGR 363 NVSF+ ++GE S+ + DIAVS+GSAC SA+ PSYVL ALGR Sbjct: 315 NVSFNYVEGE-SLIMAIKDIAVSSGSACTSASLEPSYVLRALGR 357 >gi|300312137|ref|YP_003776229.1| cysteine desulfurase NifS [Herbaspirillum seropedicae SmR1] Length = 406 Score = 451 bits (1159), Expect = e-128, Method: Compositional matrix adjust. Identities = 232/347 (66%), Positives = 266/347 (76%), Gaps = 4/347 (1%) Query: 17 QTPLQFPIYLDYSATTPVDPLVATRMFAYLTEKFGNPASSSHSFGWEAKAAVEKARKQVA 76 + L PIYLDYSATTPVDP VA +M YLT+ GNPAS SHSFGW A+AAVE AR+QVA Sbjct: 2 HSSLSLPIYLDYSATTPVDPRVAQKMIPYLTDIPGNPASRSHSFGWAAEAAVEHAREQVA 61 Query: 77 ALVGAQASEIVWTSGATESNNLALKSAAHALRAAGRGKHLITVATEHKAVLDTMHTLERA 136 ALV A EI+WTSGATESNNLA+K AA LR+ GR HLITV TEHKAVLDTM LER Sbjct: 62 ALVNADVREIIWTSGATESNNLAIKGAAEFLRSKGR--HLITVKTEHKAVLDTMRELERR 119 Query: 137 GFEVSFLQPGTDGLITVAQFAEALRPDTILASVMMVNNEIGVIQPIEELGEICAARDILF 196 GFEV++L+ G++GL+ +A F ALRPDTILASVM VNNEIGVIQ I LG+IC AR +L Sbjct: 120 GFEVTYLEVGSNGLLDLAAFEAALRPDTILASVMYVNNEIGVIQDIAALGDICRARGVLL 179 Query: 197 HVDAVQAVGKIAIDLATLKVDLMSFSAHKIYGPKGIGALFVRNNPALRLEAQIDGGGHEN 256 HVDA QA GK+AIDLA LKVDLMSFSAHK YGPKGIGAL+V P +RLEAQ+ GGGHE Sbjct: 180 HVDAAQATGKVAIDLARLKVDLMSFSAHKTYGPKGIGALYVGRKPRVRLEAQMHGGGHER 239 Query: 257 GMRSGTLPTHQIVGMGEAFALAANLQDTEAARIGVLRDRLWEGLKDLPGVVLNGSATARI 316 G+RSGTL THQIVGMGEAF LA TE RI +LRDRL G D+ +NG R+ Sbjct: 240 GLRSGTLATHQIVGMGEAFELARLAMGTENERIRMLRDRLHAGFADIEECFVNGDLERRV 299 Query: 317 PHNLNVSFD-LDGEISVAGQLLDIAVSAGSACNSANAAPSYVLTALG 362 PHNLNVSF+ ++GE S+ + D+AVS+GSAC SA+ PSYVL ALG Sbjct: 300 PHNLNVSFNYVEGE-SLLMAIKDVAVSSGSACTSASLEPSYVLRALG 345 >gi|300312171|ref|YP_003776263.1| cysteine desulfurase 2 protein [Herbaspirillum seropedicae SmR1] Length = 379 Score = 194 bits (494), Expect = 9e-51, Method: Compositional matrix adjust. Identities = 141/342 (41%), Positives = 197/342 (57%), Gaps = 12/342 (3%) Query: 24 IYLDYSATTPVDPLVATRMFAYLTEKFGNPASSSHSFGWEAKAAVEKARKQVAALVGAQA 83 IYLD +ATT P V M A E +GNP SS H G A+ + AR +VAAL+ A A Sbjct: 5 IYLDNNATTRPTPEVLAAMQACQDEAWGNP-SSKHRVGDAARQRMNSARAEVAALLAASA 63 Query: 84 SEIVWTSGATESNNLALKSAAHALRAAGRGKHLITVATEHKAVLDTMHTLERAGFEVSFL 143 +E+V+TSGATE+N++A+ A L + +H+++ EH + L + L+ G +S++ Sbjct: 64 AELVFTSGATEANHMAILGA---LALQPQRRHIVSSLVEHPSTLLLLRHLQAQGVRISWI 120 Query: 144 QPGTDGLITVAQFAEALRPDTILASVMMVNNEIGVIQPIEELGEICAARDILFHVDAVQA 203 G + + + A A+ PDT L S+M NNE GV+ PI + E+ R +LFH DAVQA Sbjct: 121 AVDEHGQLQLDELAAAITPDTALVSLMWANNETGVVFPIAQAAELARQRGVLFHTDAVQA 180 Query: 204 VGKIAIDLATLKVDLMSFSAHKIYGPKGIGALFVRNNPALRLEAQIDGGGHENGMRSGTL 263 VG++ D+A L VDL+S SAHK++GPKGIGAL+VR L + G E G R GT Sbjct: 181 VGRVPCDVAGLGVDLLSLSAHKLHGPKGIGALYVRKGIKL---PPLLFGHQERGRRGGTE 237 Query: 264 PTHQIVGMG-EAFALAANLQDTEAARIGVLRDRLWEGLKDLPGVVLNGSATARIPHNLNV 322 IVG+G A A L +A + LR R GL LP V +NG+ AR+P+ ++V Sbjct: 238 DVAGIVGLGVAAAQARAALPRMQA--VSALRQRFERGLARLPQVKINGAGAARLPNTVSV 295 Query: 323 SFDLDGEISVAGQL--LDIAVSAGSACNSANAAPSYVLTALG 362 F V +L L I S+GSAC ++ PS+VL A+G Sbjct: 296 RFGQVHAELVLERLDRLGICASSGSACTASGDMPSHVLMAMG 337 >gi|300311647|ref|YP_003775739.1| selenocysteine lyase [Herbaspirillum seropedicae SmR1] Length = 632 Score = 93.2 bits (230), Expect = 4e-20, Method: Compositional matrix adjust. Identities = 104/366 (28%), Positives = 157/366 (42%), Gaps = 39/366 (10%) Query: 8 IPITSSGQDQTPLQFPIYLDYSATTPVDPLVATRMFAYLTEKFGNPASSSHSFGWEAKAA 67 PI + PL I+ D +ATT V R+ + + N ++H A A Sbjct: 236 FPILQERVNGRPL---IWFDNAATTHKPQSVIDRISEFYRRENSNIHRAAHELAARATDA 292 Query: 68 VEKARKQVAALVGA-QASEIVWTSGATESNNLALKSAAHALRAAGRGKHLITVATEHKAV 126 E AR++V A + A +E+++ G TE+ NL KS + G G +I EH A Sbjct: 293 YEGARERVRAFLNAPDVNEVIFVRGTTEAINLVAKSWGG--KHVGAGDEIIVSHLEHHAN 350 Query: 127 LDTMHTLE-RAGFEVSFLQPGTDGLITVAQFAEALRPDTILASVMMVNNEIGVIQP---I 182 + L G ++ + G + + ++A+ L T + +V V+N +G I P I Sbjct: 351 IVPWQQLAAEKGAKLRVIPVDDSGQVLLDEYAKLLNERTRIVAVTQVSNALGTITPTKQI 410 Query: 183 EELGEICAARDILFHVDAVQAVGKIAIDLATLKVDLMSFSAHKIYGPKGIGALFVRNNPA 242 EL + AR + VD Q+V + D+ L D FS HK++GP GIGAL+ R + Sbjct: 411 VELARLAGARTL---VDGAQSVSHMRTDVQALGADFFVFSGHKVFGPTGIGALWGRRD-V 466 Query: 243 LRLEAQIDGGGH---------------ENGMRSGTLPTHQIVGMGEAFALAANLQ-DTEA 286 L GGG+ N +GT VG+G A + D A Sbjct: 467 LEDMPPWQGGGNMIADVTFEKTIFQPLPNTFEAGTGNIADAVGLGAAIDYVNRVGIDNIA 526 Query: 287 ARIGVLRDRLWEGLKDLPGVVLNGSATARIPHNLNV-SFDLDG-EISVAGQLLD---IAV 341 A L + L ++ G+ L G+A H +V SF L+G G+ L+ IAV Sbjct: 527 AYEHALLEYGMHQLGEIEGLRLIGTAA----HKASVMSFVLEGYSTEDVGKALNQEGIAV 582 Query: 342 SAGSAC 347 G C Sbjct: 583 RTGHHC 588 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.132 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,187,766 Number of extensions: 46080 Number of successful extensions: 174 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 167 Number of HSP's successfully gapped: 5 Length of query: 420 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 332 Effective length of database: 1,203,190 Effective search space: 399459080 Effective search space used: 399459080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)