BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0082.p (483 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313829|ref|YP_003777921.1| sensor histidine kinase [Herbas... 788 0.0 gi|300313666|ref|YP_003777758.1| two component sensor histidine ... 213 4e-56 gi|300310848|ref|YP_003774940.1| two component sensor histidine ... 194 2e-50 gi|300311468|ref|YP_003775560.1| oxidative stress resistance two... 119 6e-28 gi|300311514|ref|YP_003775606.1| two component sensor histidine ... 107 2e-24 >gi|300313829|ref|YP_003777921.1| sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 478 Score = 788 bits (2035), Expect = 0.0, Method: Compositional matrix adjust. Identities = 399/477 (83%), Positives = 439/477 (92%) Query: 6 LPNSLLGRLSVVMIFGVLVTQLAGGLIWAAQLRTKSEAETRVAAQHLAHSASGAIRYFMT 65 +P+SLLGRLSVVMIFGVLVTQ+AGGLIWA QLR+KSEAETR AAQHLAHSA+ AIRYFMT Sbjct: 1 MPDSLLGRLSVVMIFGVLVTQVAGGLIWAVQLRSKSEAETRTAAQHLAHSAASAIRYFMT 60 Query: 66 LPANYRPIMIQQLREMGGTRFFINANSALVPIPPIAPNSLADIATEAAAETLKSDLAFLP 125 LP NYRP+MIQQLREMGGTRFFINANS+ V + I + LA +A + AA+TL+ DL FLP Sbjct: 61 LPPNYRPLMIQQLREMGGTRFFINANSSAVAVKSIGDSRLAKLAIDVAAQTLREDLPFLP 120 Query: 126 EFHLSFAWPDNLPVSDDGLKIGDLPDSWVQHILLIKPNPAPVLVIQTQIESGKWLYLAAL 185 E L+FAWPD LPV+ DGL + DLPD+WVQHIL+ KPN APVLVIQ Q+ESG+W+YLAAL Sbjct: 121 EARLAFAWPDGLPVASDGLTVNDLPDNWVQHILMTKPNSAPVLVIQMQVESGRWIYLAAL 180 Query: 186 MPNPYFLDSSNPLSLDRVVLQGLSLAAILLLTILVVRWTTRPLAALSDAAEAFGKGETVP 245 MPNPYFLDS NPL LDR+VLQGLSLAAILLLTILVVRWTTRPLAAL+DAAEAFGKGETVP Sbjct: 181 MPNPYFLDSDNPLWLDRLVLQGLSLAAILLLTILVVRWTTRPLAALADAAEAFGKGETVP 240 Query: 246 ELPETGSREFVKTARAFSGMRERIKRYLEDRERLFVSISHDLRTPITRLKLRTELLDDDD 305 LPETGSRE+VKTARAFSGMRERIKRYLEDRERLFVSISHDLRTPITRLKLRTEL+DDD Sbjct: 241 SLPETGSREYVKTARAFSGMRERIKRYLEDRERLFVSISHDLRTPITRLKLRTELMDDDA 300 Query: 306 LRTEFHDDLDELDMMVKGALQSVKDSDIHENRTEVRLDALVARMIRDASMAGHEVAFEPS 365 +RT+FHDDLDELDMMVKGALQSVKDSDIHENRTEVRLDALV RMIRDA +AGHE+AF PS Sbjct: 301 MRTDFHDDLDELDMMVKGALQSVKDSDIHENRTEVRLDALVLRMIRDARLAGHEIAFTPS 360 Query: 366 GVNVMAKPLALKRAIGNLFDNALHYGEKVEIAIRQSQALEGDMVEIEIRDHGPGVPEDAM 425 G+NV+AKPLALKRAIGNLFDNAL+YG+KVEI+IR S EGD+VEIEIRDHGPGVPEDAM Sbjct: 361 GINVIAKPLALKRAIGNLFDNALYYGQKVEISIRPSPGAEGDLVEIEIRDHGPGVPEDAM 420 Query: 426 GSLFQPYVRLEHGRSANSGGMGLGLGIARNIVQAHGGELLLRNHPNGGLIATIIVPA 482 G+LFQPYVRLEHGR+ NSGGMGLGLGI+RNIVQAHGGELLLRNHP GGL+ATII+PA Sbjct: 421 GTLFQPYVRLEHGRAQNSGGMGLGLGISRNIVQAHGGELLLRNHPEGGLMATIILPA 477 >gi|300313666|ref|YP_003777758.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 479 Score = 213 bits (541), Expect = 4e-56, Method: Compositional matrix adjust. Identities = 149/489 (30%), Positives = 239/489 (48%), Gaps = 33/489 (6%) Query: 4 RVLPNSLLGRLSVVMIFGVLVTQLAGGLIWA-AQLRTKSEAETRVAAQHLAHSASGAIRY 62 R P +L GRL+++++ G+L Q+ G IW + T E TR+AA HLA A+ Sbjct: 12 RPWPQTLSGRLALILVAGMLAAQMLTGTIWYDMRYGTAMEMPTRLAAAHLAD----AVLI 67 Query: 63 FMTLPANYRPIMIQQLREMGGTRFFIN----ANSALVPIPPIAPNSLADIATEAAAETLK 118 PA R + L +F ++ + A + +P +AP A++ T Sbjct: 68 LDDAPATQRQALAHSLTR---AQFVLSLPDVVDRADLAVPGLAP---------ASSPTTH 115 Query: 119 SDLAFLPEFHL---SFAWPDNLPVSDDGL--KIGDLPDSWVQHILLIKPNPAPVLVIQTQ 173 +D + F A P + GL + + SW LL P + Sbjct: 116 ADQGVVDLFGTVLQQHAGPRPWKMLQLGLDDQHANAESSW--WTLLRDEFPHGRFRVLVT 173 Query: 174 IESGKWLYLAALMPNPYFLDSSNPLSLDRVV-LQGLSLAAILLLTILVVRWTTRPLAALS 232 ++ G WL + + + D + + L + A++L+ + VR +PL ++ Sbjct: 174 LKDGSWLQADIEEGQAGMSTAPHSAAFDYFMRIYALRIFAVVLIAWMAVRLAMKPLQVMA 233 Query: 233 DAAEAFGKGETVPELPETGSREFVKTARAFSGMRERIKRYLEDRERLFVSISHDLRTPIT 292 +AAE G L G E A+AF+ M+ RI + +R R ++SHDLR+PIT Sbjct: 234 EAAEKLGGNIRSAPLATDGPLEVSTAAQAFNAMQRRIIDNIAERTRFLAAVSHDLRSPIT 293 Query: 293 RLKLRTELLDDDDLRTEFHDDLDELDMMVKGALQSVKDSDIHENRTEVRLDALVARMIRD 352 R+KLRTE+L D+ + +F DLDE+D ++ L V+D D+ E + LD+L+ + D Sbjct: 294 RMKLRTEMLPDERGKEKFRKDLDEMDALISATLNVVQDIDVQERPQLIDLDSLLESLRTD 353 Query: 353 ASMAGHEVAFEPSGVNVMAKPLALKRAIGNLFDNALHYGEKVEIAIRQSQALEGDMVEIE 412 + G EV + P +L+R + NL +NA+ YG++ + ++ + D V I Sbjct: 354 QAEVGGEVTLSGHAAPLQGYPRSLRRCLQNLVENAVRYGQRARVQLQD----DADQVCIR 409 Query: 413 IRDHGPGVPEDAMGSLFQPYVRLEHGRSANSGGMGLGLGIARNIVQAHGGELLLRNHPNG 472 + D GPG+PE + + +P+ RLE R+A +GG GLGL IA +VQAH G + L N P G Sbjct: 410 VDDDGPGIPEALLERVQEPFYRLERSRNAGTGGFGLGLSIAATVVQAHRGVMKLVNRPGG 469 Query: 473 GLIATIIVP 481 GL I++P Sbjct: 470 GLRVEILIP 478 >gi|300310848|ref|YP_003774940.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 465 Score = 194 bits (492), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 111/275 (40%), Positives = 160/275 (58%), Gaps = 5/275 (1%) Query: 208 LSLAAILLLTILVVRWTTRPLAALSDAAEAFGKGETVPELPETGSREFVKTARAFSGMRE 267 L L I +L LV R TRP+ L+ AA G L ETG E + A AF+ M+ Sbjct: 187 LFLVLIAMLAWLVARMATRPIRQLAQAASRLGSDIDHAPLAETGPTEIRQAAHAFNAMQA 246 Query: 268 RIKRYLEDRERLFVSISHDLRTPITRLKLRTELLDDDDLRTEFHDDLDELDMMVKGALQS 327 RI+R ++ R + +I+HDL+TP+TR++LR E + D L+ + DDL + MV+ L Sbjct: 247 RIRRQIQHRTHMLAAITHDLQTPLTRMRLRLEKVSDPGLQQKLIDDLGVMHGMVREGLDL 306 Query: 328 VKDSDIHENRTEVRLDALVARMIRDASMAGHEVAFEPSG-VNVMAKPLALKRAIGNLFDN 386 + D E + +D+L+ + DA+ AG +VA + S VMA+P AL+R + NL DN Sbjct: 307 ARSMDSSEKIQALDIDSLLDSVCADAADAGQDVALQGSTRAFVMAQPGALRRCLTNLIDN 366 Query: 387 ALHYGEKVEIAIRQSQALEGDMVEIEIRDHGPGVPEDAMGSLFQPYVRLEHGRSANSGGM 446 A YG+ ++I ALE + I IRD GPG+PE + ++F+P+ RLE RS ++GG Sbjct: 367 ACKYGKSARVSI----ALEQQKIAIRIRDQGPGIPESELEAVFEPFYRLEASRSRDTGGT 422 Query: 447 GLGLGIARNIVQAHGGELLLRNHPNGGLIATIIVP 481 GLGL IA NI + H G+L LRN GGL + +P Sbjct: 423 GLGLTIALNIAENHHGQLRLRNLAEGGLEVLLELP 457 >gi|300311468|ref|YP_003775560.1| oxidative stress resistance two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 455 Score = 119 bits (298), Expect = 6e-28, Method: Compositional matrix adjust. Identities = 98/290 (33%), Positives = 142/290 (48%), Gaps = 30/290 (10%) Query: 208 LSLAAILLLTILVVRWTTRPLAALSDAAEAFGKGETVPELPETGSREFVKTARAFSGMRE 267 LSL + ++ L+ +PLA L+ AA A KG LPE+G E + R+F+ M Sbjct: 172 LSLIGAVFISTLI----NQPLARLTAAARAIAKGRQPEPLPESGPTEIEEANRSFNQMVA 227 Query: 268 RIKRYLEDRERLFVSISHDLRTPITRLKLRTELLD-DDDLRTEFHDDLDELDMMVKGALQ 326 + R DR + ISHDLRTP+ R++L E+ + D+ R H DL ++D ++ L Sbjct: 228 DLNRIESDRALILAGISHDLRTPLARMQLEVEMANLSDEARDGMHSDLAQMDSIIGQFLD 287 Query: 327 SVKDSDIHENRTEVRLDALVARMIRDASMAGHEVAFEPSGVNVMAKP--------LALKR 378 K D T +D +A +++D +AG+ V+ P L R Sbjct: 288 YAKPFDTSSLET---ID--LADVLQD--VAGNASRLSDIRVHTQITPDTQVAGNGTELAR 340 Query: 379 AIGNLFDNALHYGEK-----VEIAIRQSQALEGDMVEIEIRDHGPGVPEDAMGSLFQPYV 433 NL +NA YG+ EI IR +EG V +E+ DHGPGVP + L +P+ Sbjct: 341 VFSNLVENARRYGKTEGTDCAEIDIRSR--IEGHQVVVEVADHGPGVPVNECERLLRPFT 398 Query: 434 RLEHGRSANSGGMGLGLGIARNIVQAHGGELLLRNHPN--GGLIATIIVP 481 RL+ R + G GLGL I IV H G+LLL+ H GL+ I +P Sbjct: 399 RLDTAR-GQANGSGLGLAIVNRIVLKHNGKLLLKGHEETRSGLLVQITLP 447 >gi|300311514|ref|YP_003775606.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 473 Score = 107 bits (268), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 91/293 (31%), Positives = 143/293 (48%), Gaps = 22/293 (7%) Query: 208 LSLAAILLLTILVVRWTTRPLAALSDAAEAFGKGETVPEL-PETGSR--EFVKTARAFSG 264 ++L A L+ +L+ + + P+ L +A G EL P +R E R F Sbjct: 170 VALLASLVFALLLAWYFSHPIGHLKNAFNQAANGNLDLELEPVMSARNDELADLGRDFDV 229 Query: 265 MRERIKRYLEDRERLFVSISHDLRTPITRLKL-----RTELLDDDDLRTE-FHDDLDELD 318 M +++ +E + RL +SH+LR+PI RL++ R + D DL E + +D Sbjct: 230 MTAKLRTLMESQRRLLHDVSHELRSPIARLQVAIGLARVQDKDKLDLTLERIERESVRMD 289 Query: 319 MMVKGALQ-SVKDSDIHENRTE-VRLDALVARMIRDASMAGHEVAFE-----PSGVNVMA 371 +V L S ++++ + E + ++A++ MI DAS + V V Sbjct: 290 KLVGELLTLSRLEANVPGSMEEPIAIEAMLGDMINDASFEAEAIGKHVDDQLDCRVQVKG 349 Query: 372 KPLALKRAIGNLFDNALHY---GEKVEIAIRQSQALEGDMVEIEIRDHGPGVPEDAMGSL 428 P L RAI N+ NA+ + G V+I + + ++ + I D GPGVPE+ M S+ Sbjct: 350 NPELLYRAIENVLRNAIKHTAPGTTVQIHTQLDEI--NKLLHLSILDRGPGVPEEEMNSI 407 Query: 429 FQPYVRLEHGRSANSGGMGLGLGIARNIVQAHGGELLLRNHPNGGLIATIIVP 481 F+ + R +S NS G GLGL IAR IV+AH G + N NGGL I +P Sbjct: 408 FELFFRSSRTKS-NSSGHGLGLSIARRIVEAHQGFIQAANRSNGGLRVDITLP 459 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.137 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,502,551 Number of extensions: 63875 Number of successful extensions: 354 Number of sequences better than 1.0e-08: 40 Number of HSP's gapped: 319 Number of HSP's successfully gapped: 40 Length of query: 483 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 394 Effective length of database: 1,198,455 Effective search space: 472191270 Effective search space used: 472191270 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)