BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0062.p (429 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311827|ref|YP_003775919.1| sugar permease of the major fac... 739 0.0 gi|300310790|ref|YP_003774882.1| major facilitator superfamily p... 656 0.0 gi|300312423|ref|YP_003776515.1| proline/betaine transporter pro... 308 4e-85 gi|300309575|ref|YP_003773667.1| proline/betaine transporter pro... 285 6e-78 gi|300309357|ref|YP_003773449.1| major facilitator superfamily (... 279 4e-76 >gi|300311827|ref|YP_003775919.1| sugar permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 739 bits (1908), Expect = 0.0, Method: Compositional matrix adjust. Identities = 365/423 (86%), Positives = 394/423 (93%), Gaps = 1/423 (0%) Query: 1 MTSPTIASSSKFATVLRVTSGNFMEMFDFFLFGFYASYISKAFFPSGDEFASLMLTFMTF 60 MT+ T AS SKF+TVLRVTSGNFMEMFDFFLFGFYA++ISKAFFPS +EFASLMLTFMTF Sbjct: 1 MTTST-ASQSKFSTVLRVTSGNFMEMFDFFLFGFYATHISKAFFPSDNEFASLMLTFMTF 59 Query: 61 GAGFLMRPLGAIILGAYVDRVGRRQGLIVTLALMALGTVLIAFVPGYATIGLLAPALVLI 120 GAGFLMRPLGAIILGAYVDRVGRRQGLIVTLALMALGT+LIAFVP YATIG LAP LVLI Sbjct: 60 GAGFLMRPLGAIILGAYVDRVGRRQGLIVTLALMALGTMLIAFVPSYATIGALAPLLVLI 119 Query: 121 GRLLQGFSAGVELGGVSVYLAEMATPGRKGFYVSWQSASQQVAIIFAAAIGYGLNIWLTK 180 GRLLQGFSAGVELGGVSVYLAEMATPG KGFYVSWQSASQQVAII AAAIGY L+ LT Sbjct: 120 GRLLQGFSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQVAIIVAAAIGYLLSTSLTP 179 Query: 181 DQVGDWGWRVPFFIGCMIVPVLFVIRRSLQETEEFLARKRKPNLNEIFRSMVENWKLVIA 240 +V WGWR+PFFIGC+IVPVLFVIRRSLQETEEF+ RK +P+ +IFRSM+ENWKLVIA Sbjct: 180 AEVSGWGWRIPFFIGCLIVPVLFVIRRSLQETEEFMRRKHRPSTGQIFRSMIENWKLVIA 239 Query: 241 GMMLVSMTTVSFYLITVYTPTFGKNVLKLSTESALIVTFCVGISNFIWLPVMGALSDRIG 300 GMMLVSMTTVSFYLITVYTPTFGKNVLKLSTE++LIVT CVG+SNFIWLP+MGALSDR+G Sbjct: 240 GMMLVSMTTVSFYLITVYTPTFGKNVLKLSTEASLIVTLCVGLSNFIWLPIMGALSDRVG 299 Query: 301 RRPLLLVFTILTILTAYPAMTWLVGDATFGKMLVVELWLSFLYASYNGAMVVALTEVMPV 360 RRPLL+ FT+LTILTAYPA+ WLV DA+F KML+VELWLSFLYASYNGAMVVALTEVMP Sbjct: 300 RRPLLVTFTVLTILTAYPAVNWLVHDASFAKMLIVELWLSFLYASYNGAMVVALTEVMPA 359 Query: 361 DVRTAGFSLAYSLATAVFGGFTPAIATGLIEITHDKAAPGLWMSFAAVCGLIATLVLYRK 420 DVRTAGFSLAYSLATAVFGGFTPAIATGLIE THDKAAPG+WMS AAVCGLIATL+LYR Sbjct: 360 DVRTAGFSLAYSLATAVFGGFTPAIATGLIEYTHDKAAPGIWMSTAAVCGLIATLILYRN 419 Query: 421 KSL 423 K++ Sbjct: 420 KAV 422 >gi|300310790|ref|YP_003774882.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 432 Score = 656 bits (1693), Expect = 0.0, Method: Compositional matrix adjust. Identities = 326/423 (77%), Positives = 371/423 (87%) Query: 2 TSPTIASSSKFATVLRVTSGNFMEMFDFFLFGFYASYISKAFFPSGDEFASLMLTFMTFG 61 T+ T A SK +TVLRVTSGNF+EMFDFFL+GFYASYISK FFPS +EF SL+LTF TFG Sbjct: 3 TTTTNAKHSKLSTVLRVTSGNFLEMFDFFLYGFYASYISKTFFPSDNEFVSLILTFGTFG 62 Query: 62 AGFLMRPLGAIILGAYVDRVGRRQGLIVTLALMALGTVLIAFVPGYATIGLLAPALVLIG 121 AGFLMRPLGAIILG+Y+DRVGRRQGLIVTLA+MA+GT+LIAFVPGYATIG+ AP LVL G Sbjct: 63 AGFLMRPLGAIILGSYIDRVGRRQGLIVTLAIMAIGTLLIAFVPGYATIGVAAPILVLTG 122 Query: 122 RLLQGFSAGVELGGVSVYLAEMATPGRKGFYVSWQSASQQVAIIFAAAIGYGLNIWLTKD 181 RLLQGFSAGVELGGVSVYLAEMATPG KGFYVSWQSASQQ AI+ +A IGY ++ L+ Sbjct: 123 RLLQGFSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQAAIMASALIGYLVSQSLSPV 182 Query: 182 QVGDWGWRVPFFIGCMIVPVLFVIRRSLQETEEFLARKRKPNLNEIFRSMVENWKLVIAG 241 Q+ DWGWR+PFFIGCMIVP +F+IRRSLQET+EFLARKR P+ EI S+ +NWK+V+AG Sbjct: 183 QISDWGWRIPFFIGCMIVPAIFIIRRSLQETDEFLARKRHPSFGEIMSSIAQNWKIVVAG 242 Query: 242 MMLVSMTTVSFYLITVYTPTFGKNVLKLSTESALIVTFCVGISNFIWLPVMGALSDRIGR 301 MM+V MTTVSFYLITVYTPTFGKNVLKLS +LIVTFCVG+SNF WLP+MGALSDR+GR Sbjct: 243 MMMVVMTTVSFYLITVYTPTFGKNVLKLSASDSLIVTFCVGLSNFCWLPIMGALSDRVGR 302 Query: 302 RPLLLVFTILTILTAYPAMTWLVGDATFGKMLVVELWLSFLYASYNGAMVVALTEVMPVD 361 RP+L+ FT+LT+LTAYP M+WLV D +F +L+ ELWLSFLYASYNGA VVALTEVMP Sbjct: 303 RPVLVAFTLLTLLTAYPVMSWLVSDISFKNLLITELWLSFLYASYNGAAVVALTEVMPAH 362 Query: 362 VRTAGFSLAYSLATAVFGGFTPAIATGLIEITHDKAAPGLWMSFAAVCGLIATLVLYRKK 421 VRT GFSLAYSLATA+FGGFTP ++T LIE + DKAAPG WMSFAAVCGL+ATLVLYR K Sbjct: 363 VRTVGFSLAYSLATAIFGGFTPLVSTWLIETSGDKAAPGYWMSFAAVCGLLATLVLYRGK 422 Query: 422 SLA 424 S A Sbjct: 423 SAA 425 >gi|300312423|ref|YP_003776515.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 427 Score = 308 bits (790), Expect = 4e-85, Method: Compositional matrix adjust. Identities = 174/397 (43%), Positives = 242/397 (60%), Gaps = 7/397 (1%) Query: 21 GNFMEMFDFFLFGFYASYISKAFFPSGDEFASLMLTFMTFGAGFLMRPLGAIILGAYVDR 80 GN +E FD ++G +A I+K FFP+GD+ SL+LTF TFG F MRPLGA++LGAY DR Sbjct: 27 GNALEWFDILIYGAFAVVIAKQFFPTGDDSVSLLLTFATFGVSFFMRPLGAVVLGAYSDR 86 Query: 81 VGRRQGLIVTLALMALGTVLIAFVPGYATIGLLAPALVLIGRLLQGFSAGVELGGVSVYL 140 GR+ L++++ LM +GT +IAF+P YA+IGLLAPA + +G+++QGFSAG E G + +L Sbjct: 87 AGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLAPAGIALGKMIQGFSAGGEFGSSTAFL 146 Query: 141 AEMATPGRKGFYVSWQSASQQVAIIFAAAIGYGLNIWLTKDQVGDWGWRVPFFIGCMIVP 200 E A P R+GF+ SWQ ASQ ++++ AA G LN LT +Q+ WGWRVPF G +I P Sbjct: 147 VEHA-PHRRGFFSSWQVASQGISLLLAALFGAVLNNMLTPEQLASWGWRVPFIFGLLIAP 205 Query: 201 VLFVIRRSLQETEEFLARKRKPN--LNEIFRSMVENWKLVIAGMMLVSMTTVSFYLITVY 258 IRR L E EF K N L + F + +L+I G V M TVS YL ++Y Sbjct: 206 AGIYIRRHLDEAPEFKESSEKTNAPLRDTFAH--QKMRLLI-GAGSVIMATVSVYL-SLY 261 Query: 259 TPTFGKNVLKLSTESALIVTFCVGISNFIWLPVMGALSDRIGRRPLLLVFTILTILTAYP 318 PT+ L L S+ G+ FI P++GALSD+IGR P ++ + L I+ YP Sbjct: 262 IPTYAVKQLGLPAWSSFAAMSVAGLIMFIGSPLVGALSDKIGRTPFMITSSALYIVLTYP 321 Query: 319 AMTWLVGDATFGKMLVVELWLSFLYASYNGAMVVALTEVMPVDVRTAGFSLAYSLATAVF 378 +L F ++L+++ + L Y AM L ++ PV R G SLAY++A +F Sbjct: 322 MFVFLTNSPGFLQLLLLQTVIGVLMTMYFAAMPALLADIFPVATRGTGMSLAYNIAVTIF 381 Query: 379 GGFTPAIATGLIEITHDKAAPGLWMSFAAVCGLIATL 415 GGF I T LI+ T DK + ++ F AV LIA+L Sbjct: 382 GGFAGLIITWLIDFTGDKLSVSYFVIFGAVLSLIASL 418 >gi|300309575|ref|YP_003773667.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 285 bits (728), Expect = 6e-78, Method: Compositional matrix adjust. Identities = 157/412 (38%), Positives = 233/412 (56%), Gaps = 5/412 (1%) Query: 5 TIASSSKFATVLRVTSGNFMEMFDFFLFGFYASYISKAFFPSGDEFASLMLTFMTFGAGF 64 ++++ + ++ GN +E FDF ++ F+A I+K FFP+G++ SL+L TFG GF Sbjct: 13 SVSNQQRRRAIVATVIGNGLEWFDFTVYSFFAVIIAKLFFPTGNDLTSLLLAVATFGVGF 72 Query: 65 LMRPLGAIILGAYVDRVGRRQGLIVTLALMALGTVLIAFVPGYATIGLLAPALVLIGRLL 124 MRP+G I+LG Y DRVGR+ L VT+ LMA+GT +I P Y +IGL AP ++++ RLL Sbjct: 73 FMRPVGGILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPTYESIGLFAPLIIVVARLL 132 Query: 125 QGFSAGVELGGVSVYLAEMATPGRKGFYVSWQSASQQVAIIFAAAIGYGLNIWLTKDQVG 184 QGFSAG E+G + +L E A + +Y SW AS VA++ AA+G + L + Sbjct: 133 QGFSAGGEMGSATAFLTEYAPERERAYYSSWIQASIGVAVLLGAAVGTFVTASLDAAALA 192 Query: 185 DWGWRVPFFIGCMIVPVLFVIRRSLQETEEFLARKRKPN--LNEIFRSMVENWKLVIAGM 242 WGWR+PF +G +I PV + IR L+ET FL K K + L E+ R+ A Sbjct: 193 SWGWRLPFLLGIIIGPVGYYIRHHLEETPTFLEEKDKTDSPLKEVIRNFPRETS---ASF 249 Query: 243 MLVSMTTVSFYLITVYTPTFGKNVLKLSTESALIVTFCVGISNFIWLPVMGALSDRIGRR 302 +V + TV Y++ Y PT+ VLKL + I G+ + P++G L+DRIGRR Sbjct: 250 SMVILWTVCTYVLLFYMPTYSVKVLKLPQTTGFIAGMVGGLCIMVLAPIVGRLADRIGRR 309 Query: 303 PLLLVFTILTILTAYPAMTWLVGDATFGKMLVVELWLSFLYASYNGAMVVALTEVMPVDV 362 PLL L ++ AYP ++ + +L+ +L L A+Y G ++ A +E+ P V Sbjct: 310 PLLSGSAALILMLAYPMFAYINSAPSLSTLLIFQLVFGTLIAAYTGPILAAFSELFPAKV 369 Query: 363 RTAGFSLAYSLATAVFGGFTPAIATGLIEITHDKAAPGLWMSFAAVCGLIAT 414 + G S+AY+LA +FGGF T LI T AP ++ AA + T Sbjct: 370 LSTGLSVAYNLAVTIFGGFASFFITWLIAATGSTMAPAFYVMIAAAISFVGT 421 >gi|300309357|ref|YP_003773449.1| major facilitator superfamily (MFS) transporter protein [Herbaspirillum seropedicae SmR1] Length = 437 Score = 279 bits (713), Expect = 4e-76, Method: Compositional matrix adjust. Identities = 154/417 (36%), Positives = 237/417 (56%), Gaps = 3/417 (0%) Query: 10 SKFATVLRVTSGNFMEMFDFFLFGFYASYISKAFFPSGDEFASLMLTFMTFGAGFLMRPL 69 +K V+ GN +E +DF ++GF+++ I++ FFP+ +E+ SL++ TFG GF MRP+ Sbjct: 6 NKQRQVIAAIVGNALEWYDFIVYGFFSAIIARLFFPADNEYTSLLVALATFGIGFFMRPV 65 Query: 70 GAIILGAYVDRVGRRQGLIVTLALMALGTVLIAFVPGYATIGLLAPALVLIGRLLQGFSA 129 G ++LG Y DR GR+ + V + LM L +IAF P YATIG+ AP ++++ R+LQGF+ Sbjct: 66 GGVLLGLYADRKGRKAAMQVIIVLMTLAIAIIAFTPTYATIGIAAPIMIVVARMLQGFAT 125 Query: 130 GVELGGVSVYLAEMATPGRKGFYVSWQSASQQVAIIFAAAIGYGLNIWLTKDQVGDWGWR 189 G E + +L E A ++G Y SWQ Q +A+ A +G + L+ + + WGWR Sbjct: 126 GGEYSSSTAFLVESAPAHKRGLYGSWQLVGQCLAVFCGAGMGALVTRNLSPEALDSWGWR 185 Query: 190 VPFFIGCMIVPVLFVIRRSLQETEEFLARKRKPNLNEI--FRSMVENWKLVIAGMMLVSM 247 +PF +G +I PV IRR + ET++FL KPN I + N + V+ M V Sbjct: 186 LPFVLGLLIGPVGLWIRRHMHETDDFLESANKPNAAPIKLLDVVKTNLRGVLVSMGQVIN 245 Query: 248 TTVSFYLITVYTPTFGKNVLKLSTESALIVTFCVGISNFIWLPVMGALSDRIGRRPLLLV 307 TV+FY++ V PTF L L + A +V +P GALSDR+GRR +LL Sbjct: 246 GTVAFYVVLVNMPTFANKQLGLPLDQAFMVQMIAVALLTAVIPFAGALSDRLGRRTVLLY 305 Query: 308 FTILTILTAYPAMTWLVGDATFGKMLVVELWLSFLYASYNGAMVVALTEVMPVDVRTAGF 367 ++ +L YP W+ + ++LV+++ L + + G M AL+E P VR+ G Sbjct: 306 SSLAFLLMVYPLFVWVSAAPSLPRLLVMQVLLCIVIGANFGPMPTALSEQFPTRVRSTGL 365 Query: 368 SLAYSLATAVFGGFTPAIATGLIEITHDKAAPGLWMSFAAVCGLIATLVLYRKKSLA 424 ++AY+LA +FGGF P I T L + + AP ++ FAA G+ A + R +S A Sbjct: 366 AVAYNLAAMLFGGFAPFIVTWLTKTSGSPVAPAWYVLFAACIGVSAAWAM-RDQSPA 421 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.141 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,268,858 Number of extensions: 51977 Number of successful extensions: 337 Number of sequences better than 1.0e-08: 16 Number of HSP's gapped: 304 Number of HSP's successfully gapped: 16 Length of query: 429 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 341 Effective length of database: 1,203,190 Effective search space: 410287790 Effective search space used: 410287790 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)