BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0043.p (300 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310099|ref|YP_003774191.1| hypothetical protein Hsero_0764... 66 3e-12 >gi|300310099|ref|YP_003774191.1| hypothetical protein Hsero_0764 [Herbaspirillum seropedicae SmR1] Length = 312 Score = 66.2 bits (160), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 60/196 (30%), Positives = 89/196 (45%), Gaps = 16/196 (8%) Query: 45 DQKWGAALLWQQSQAVNVHC--------VRSLYTGSRLEALEECAPFLVIIE----PEK- 91 D+ GA LL +QA H SL+ G R EAL E AP L+ + P + Sbjct: 20 DRGHGAYLLLDSAQAEGSHLKLQAMRLPYASLFDGKREEALPEIAPLLISLSGLDAPRRE 79 Query: 92 RLSALQGLLSRTDHKPMLTFVQSPLDLFALQRHLVRFSQIETPDTLVFPLRFADSICLPE 151 RL++ L+ + P L++ +SPL L A HL F Q+ D +R+ D+ LP Sbjct: 80 RLASWISQLAFS--APCLSWYESPLPLQAFAAHLRNFHQVGLSDGQAMMMRWYDTRILPI 137 Query: 152 ILDVFTLEQRAAILSGVTAWHYVDRRGGVVTIAGTCNDADLYVTPMYGEIDRIPFSDSQY 211 T EQR+ G+ + YVDR G TI ++A P + + D Q+ Sbjct: 138 WAQALTPEQRSLFTGGMFSIAYVDRFGEAKTIH-QASEAGPPSAPPPLDQPLVTLDDQQF 196 Query: 212 VQLFDSSEADGILNAL 227 L D+ + D ++N L Sbjct: 197 GLLVDAGDLDTLVNHL 212 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.135 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 826,298 Number of extensions: 30984 Number of successful extensions: 65 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 64 Number of HSP's successfully gapped: 1 Length of query: 300 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 215 Effective length of database: 1,217,395 Effective search space: 261739925 Effective search space used: 261739925 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)