BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0036.p (229 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311843|ref|YP_003775935.1| transmembrane sensor histidine ... 191 3e-50 gi|300309980|ref|YP_003774072.1| two component transmembrane sen... 62 4e-11 gi|300313666|ref|YP_003777758.1| two component sensor histidine ... 58 5e-10 gi|300311751|ref|YP_003775843.1| signal transduction histidine k... 55 4e-09 gi|300310848|ref|YP_003774940.1| two component sensor histidine ... 55 7e-09 >gi|300311843|ref|YP_003775935.1| transmembrane sensor histidine kinase transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 468 Score = 191 bits (486), Expect = 3e-50, Method: Compositional matrix adjust. Identities = 98/161 (60%), Positives = 122/161 (75%), Gaps = 1/161 (0%) Query: 1 LLSAQHDDEGRRHHLDRLRTRTRQLGALVNQLINHAMVQHRSDTAAPESVDLAQLVRQEM 60 LL+ + ++E R+ HL LR RTR LGALVNQLINHAMVQHR+D++ +DL +LVR +M Sbjct: 269 LLATEDNEEKRQRHLTLLRERTRNLGALVNQLINHAMVQHRNDSSLQARIDLGELVRSQM 328 Query: 61 ADMLSHQERLL-DLAMEAPEQPCLIMGDAISLREAVKNVLGNALQYGAASFLHVQLERKE 119 ++LS++ R DL+++ P PCLI GDAISL EA+KN+L NALQYGA L V+LE Sbjct: 329 TEVLSNRSRTPHDLSLDVPPLPCLIRGDAISLGEAIKNILDNALQYGAPGLLQVRLESDG 388 Query: 120 TQIELRVIDDGPGIPEADWERIRKPFSPRSGERMGASLGLS 160 + LR IDDGPGIP ADWER+RKPFSPR +RMGASLGLS Sbjct: 389 QRHWLRFIDDGPGIPAADWERLRKPFSPRGEQRMGASLGLS 429 >gi|300309980|ref|YP_003774072.1| two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 390 Score = 62.0 bits (149), Expect = 4e-11, Method: Compositional matrix adjust. Identities = 56/201 (27%), Positives = 91/201 (45%), Gaps = 16/201 (7%) Query: 5 QHDDEGRRHHLDRLRTRTRQLGALVNQLINHAMVQHR-SDTAAPESVDLAQLVRQEMAD- 62 Q D R L++L + LVNQL++ A +++ S + V+L +L R + D Sbjct: 177 QDDQVDVRRSLEQLAKSSETATRLVNQLLSLARAENQQSQSGGMIPVELNELARSVVQDW 236 Query: 63 -MLSHQERLLDLAMEAPEQPCLIMGDAISLREAVKNVLGNALQY-----GAASFLHVQLE 116 +S +R+ DL E P+ P LI G+ + LRE + N+L NAL Y + + + Sbjct: 237 VPMSFTQRI-DLGFEQPDHPILITGNPVMLRELLSNLLDNALHYTPHDGNGRVTVRARTD 295 Query: 117 RKETQIELRVIDDGPGIPEADWERIRKPF-----SPRSGERMGASLGLSPRSGERMGASL 171 L V D+GPGIP A+ + + F +P +G +G L + ++ A+L Sbjct: 296 EGGGLAILEVEDNGPGIPVAERANVFERFYRILGTPSTGSGLGLGLAIVREIAQQHEAAL 355 Query: 172 GSNCCACPRWRKWNCSACRTR 192 + PR C R Sbjct: 356 --EITSNPRSTDPKYPGCVFR 374 >gi|300313666|ref|YP_003777758.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 479 Score = 58.2 bits (139), Expect = 5e-10, Method: Compositional matrix adjust. Identities = 48/149 (32%), Positives = 74/149 (49%), Gaps = 13/149 (8%) Query: 16 DRLRTRTRQLGALVNQLINHAMVQHRSDTAAPESVDLAQLVRQEMADMLSHQERLLDLAM 75 ++ R ++ AL++ +N +VQ P+ +DL L+ D + Sbjct: 309 EKFRKDLDEMDALISATLN--VVQDIDVQERPQLIDLDSLLESLRTDQAEVGGEVTLSGH 366 Query: 76 EAPEQPCLIMGDAISLREAVKNVLGNALQYGAASFLHVQLERKETQIELRVIDDGPGIPE 135 AP Q G SLR ++N++ NA++YG + VQL+ Q+ +RV DDGPGIPE Sbjct: 367 AAPLQ-----GYPRSLRRCLQNLVENAVRYGQRA--RVQLQDDADQVCIRVDDDGPGIPE 419 Query: 136 ADWERIRKPFS----PRSGERMGASLGLS 160 A ER+++PF R+ G LGLS Sbjct: 420 ALLERVQEPFYRLERSRNAGTGGFGLGLS 448 >gi|300311751|ref|YP_003775843.1| signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 446 Score = 55.5 bits (132), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 47/167 (28%), Positives = 84/167 (50%), Gaps = 6/167 (3%) Query: 9 EGRRHHLDRLRTRTRQLGALVNQLINHAMVQHRSDTAAPESVDLAQLVRQEMADMLS-HQ 67 E R L RL T ++ LV QL+ A Q + AA + L Q++R+ + ++L Q Sbjct: 260 ETARERLTRLETGLQRARQLVTQLLALARAQTPAG-AALQPCSLHQVLREVIEELLPLAQ 318 Query: 68 ERLLDLAMEAPEQPCLIMGDAISLREAVKNVLGNALQYG-AASFLHVQLERKETQIELRV 126 + +DL ++ C ++ L + ++N++ NA+ Y A + +++ER + L + Sbjct: 319 CKQIDLGLDGERDAC-VLTQPQELAQLLRNLIDNAIAYTPAGGAVSLRMERHAQWVALEI 377 Query: 127 IDDGPGIPEADWERIRKPFS--PRSGERMGASLGLSPRSGERMGASL 171 D GPGI ++ ER+ +PF SG+ G L + R+G +L Sbjct: 378 CDTGPGIAASERERVFQPFYRIDPSGQGSGLGLAIVQSIARRLGVTL 424 >gi|300310848|ref|YP_003774940.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 465 Score = 54.7 bits (130), Expect = 7e-09, Method: Compositional matrix adjust. Identities = 46/164 (28%), Positives = 84/164 (51%), Gaps = 24/164 (14%) Query: 15 LDRLRTRTRQLG--ALVNQLIN-----HAMVQHRSDTA-------APESVDLAQLVRQEM 60 L R+R R ++ L +LI+ H MV+ D A +++D+ L+ Sbjct: 270 LTRMRLRLEKVSDPGLQQKLIDDLGVMHGMVREGLDLARSMDSSEKIQALDIDSLLDSVC 329 Query: 61 ADMLSHQERLLDLAMEAPEQPCLIMGDAISLREAVKNVLGNALQYGAASFLHVQLERKET 120 AD + D+A++ + +M +LR + N++ NA +YG ++ + + LE++ Sbjct: 330 ADAADAGQ---DVALQGSTR-AFVMAQPGALRRCLTNLIDNACKYGKSARVSIALEQQ-- 383 Query: 121 QIELRVIDDGPGIPEADWERIRKPF----SPRSGERMGASLGLS 160 +I +R+ D GPGIPE++ E + +PF + RS + G LGL+ Sbjct: 384 KIAIRIRDQGPGIPESELEAVFEPFYRLEASRSRDTGGTGLGLT 427 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.133 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 664,452 Number of extensions: 25537 Number of successful extensions: 140 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 147 Number of HSP's successfully gapped: 8 Length of query: 229 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 147 Effective length of database: 1,231,600 Effective search space: 181045200 Effective search space used: 181045200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)