BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0035.p (453 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309610|ref|YP_003773702.1| cation/multidrug efflux protein... 419 e-118 gi|300309819|ref|YP_003773911.1| acriflavin resistance transmemb... 119 4e-28 gi|300310690|ref|YP_003774782.1| cation/multidrug efflux pump pr... 114 2e-26 gi|300310853|ref|YP_003774945.1| cation/multidrug efflux pump pr... 112 4e-26 gi|300313893|ref|YP_003777985.1| cation/multidrug efflux pump pr... 110 3e-25 >gi|300309610|ref|YP_003773702.1| cation/multidrug efflux protein [Herbaspirillum seropedicae SmR1] Length = 1047 Score = 419 bits (1078), Expect = e-118, Method: Compositional matrix adjust. Identities = 220/465 (47%), Positives = 297/465 (63%), Gaps = 24/465 (5%) Query: 1 VLVTPGDNLQVRVSGAFHSPKELEEMQLRANGTTFRLGDFAVVKREYVDPPVTKMRFNGK 60 ++ T D + VR +G F + + LEE ++ N +FRLGD A + R Y +P MR NG+ Sbjct: 225 LITTGNDRVYVRPTGQFDNLRALEEKIIQINNRSFRLGDIATITRGYDEPQEQAMRANGQ 284 Query: 61 EAIGLGVSMEKGGDIIALGRNLDRMSDELRTKLPVGIELERVSDQPKIVADSVGEFIHTL 120 +G+GV+M+ GGD+I LG+ LD ++ELR +LP G+ L VS P V SV +F+ + Sbjct: 285 PVLGIGVTMQPGGDVIRLGKALDARTEELRKRLPAGLTLTAVSSMPHAVEKSVDDFLEAV 344 Query: 121 VEAVLIVLAVSFIALGLHTRPLRIDVRPGLVVALTIPLVLAVTFLFMRIFDIDLHKISLG 180 EAV IVL VS ++LG +R G+VV ++IP+VLAVT LFM +FDI LHK+SLG Sbjct: 345 AEAVAIVLVVSLVSLG---------IRTGMVVVISIPIVLAVTSLFMYLFDIGLHKVSLG 395 Query: 181 ALIIALGLLVDDAIIAVEMMVRKMEEGLSRFDAATFAYTSTAIPMLTGTLITAAGFLPIG 240 L++ALGLLVDDAIIAVEMM K+E+G +R AA FAYTSTA PMLTGTL+T AGFLPI Sbjct: 396 TLVLALGLLVDDAIIAVEMMAVKLEQGWNRRRAAAFAYTSTAFPMLTGTLVTVAGFLPIA 455 Query: 241 LAKSAAGEYTFSMFSVNALALLISWLVAVVFTPYIGVLLLKVKPAANAD---------GH 291 LAKS GEYT S+F V+A+ALL+SWL AVV P +G +LK KP A D GH Sbjct: 456 LAKSGTGEYTRSIFEVSAIALLLSWLAAVVLIPLLGYHMLKEKPGALTDEQIAANPHAGH 515 Query: 292 ------HELFDTPFYTRFRKLVTWCVEWRKTTILLTLTAFMLGLLGFRFIEQQFFPDSSR 345 H ++ T YT R+ V WCV +R ++ T F+ L F + QQFFP S R Sbjct: 516 DEEDHDHPIYQTRIYTWLRRSVAWCVAFRGRVVIATTVLFIGSLAAFALVPQQFFPSSDR 575 Query: 346 PELMIELWLPEGSAFKATEAQAKKFEAFIRKQDGVESVTSYVGTGSPRFYLPLDQIFPQT 405 PEL+I+L LPE ++F+AT+ +A + EA ++ + ++ S+VG G+PRFYLPLDQ Sbjct: 576 PELLIDLHLPERASFEATQREAVRMEALLKDRPQIDHYVSFVGVGAPRFYLPLDQQLASP 635 Query: 406 NVSQIVVLPKDLKTRESLRLKIVDAFKNDFPGVRGRXDAFKNDFP 450 N +Q VV K ++ RE+L + + + F GVR R +N P Sbjct: 636 NFAQFVVTAKSVEDREALARALEEPLRRQFSGVRTRLTRLENGPP 680 >gi|300309819|ref|YP_003773911.1| acriflavin resistance transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1048 Score = 119 bits (299), Expect = 4e-28, Method: Compositional matrix adjust. Identities = 113/396 (28%), Positives = 190/396 (47%), Gaps = 36/396 (9%) Query: 11 VRVSGAFHSPKELEEMQLRAN--GTTFRLGDFAVVK--REYVDPPVTKMRFNGKEAIGLG 66 V V+G SP++ + LRAN G T RL D A V+ + D T R +GK + +G Sbjct: 234 VVVNGQLTSPEQFGNIILRANANGATVRLRDVARVEVGGQSYD---TSGRLDGKPSSFIG 290 Query: 67 VSMEKGGDIIALGRNLDRMSDELRTKLPVGIELERVSDQPKIVADSVGEFIHTLVEAVLI 126 V + + + + + +EL P G++ D K V S+ E + TL+EA+++ Sbjct: 291 VQLSPTANALGTATAVHKKMEELAKYFPTGVKYTIPYDTSKFVKISIEEVVKTLIEAIVL 350 Query: 127 VLAVSFIALGLHTRPLRIDVRPGLVVALTIPLVLAVTFLFMRIFDIDLHKISLGALIIAL 186 V V F+ L + +R + P +VV P+ L TF + +F ++ +++ +++A+ Sbjct: 351 VFLVMFLFL----QNIRYTIIPTIVV----PVALLGTFATLLLFGFSINVLTMFGMVLAI 402 Query: 187 GLLVDDAIIAVEMMVRKM-EEGLSRFDAATFAYTSTAIPMLTGTLITAAGFLPIGLAKSA 245 G+LVDDAI+ VE + R M EEGLS DA A + ++ TL+ A F+P+ A Sbjct: 403 GILVDDAIVVVENVERIMSEEGLSPADATRKAMGQISGALIGITLVLIAVFIPMAFFGGA 462 Query: 246 AGEYTFSMFSVNALALLI-SWLVAVVFTPYIGVLLLKVKPAANADGHHELFDTPFYTRF- 303 G + FS++ +A ++ S L+A TP + +L KP HHE F+ F Sbjct: 463 VGA-IYRQFSLSMVASMVFSVLMAFTLTPALCATIL--KPVEKGH-HHE--KKGFFGWFN 516 Query: 304 RKLVTWCVEWR-----------KTTILLTLTAFMLGLLGFRFIEQQFFPDSSRPELMIEL 352 RK + ++ + I+ L +GLL R + F P+ + ++ + Sbjct: 517 RKFNSTASGYQGIIGGMLNKAGRYMIVYVLIVACVGLLYVR-LPASFLPNEDQGYIITNV 575 Query: 353 WLPEGSAFKATEAQAKKFEAFIRKQDGVESVTSYVG 388 LP G++ T A K+ E + K VE + +G Sbjct: 576 QLPPGASTSRTTAVVKQIEEYYLKDPAVEHAIAVLG 611 >gi|300310690|ref|YP_003774782.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1049 Score = 114 bits (285), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 103/357 (28%), Positives = 175/357 (49%), Gaps = 38/357 (10%) Query: 56 RFNGKEAIGLGVSMEKGGDIIALGRNLDRMSDELRTKLPVGIELERVSDQPKIVADSVGE 115 R+NGK A GL + + G + + + +D+ EL P G+++++ D V S+ E Sbjct: 280 RYNGKPAAGLAIKLATGANALDTVKAIDKRVAELEKFFPTGMKVQKPYDTTPFVRISIEE 339 Query: 116 FIHTLVEAVLIVLAVSFIALGLHTRPLRIDVRPGLVVALTIPLVLAVTFLFMRIFDIDLH 175 + TL+EAV++V V ++ L + R L+ + +P+VL TF + +F ++ Sbjct: 340 VVRTLIEAVVLVFLVMYLFLQ--------NFRATLIPTIAVPVVLLGTFGVLAVFGFTIN 391 Query: 176 KISLGALIIALGLLVDDAIIAVEMMVRKM-EEGLSRFDAATFAYTSTAIPMLTGTLITAA 234 +++ A+++A+GLLVDDAI+ VE + R M EEGL A + + ++ L+ AA Sbjct: 392 TLTMFAMVLAIGLLVDDAIVVVENVERVMSEEGLPPKQATRKSMGQISGALVGVALVLAA 451 Query: 235 GFLPIGLAKSAAGEYTFSMFSVNAL-ALLISWLVAVVFTPYIGVLLLKVKPAANADGHHE 293 F+P+ + G + FS+ + A+ +S LVA+V TP + LL KPAA H Sbjct: 452 VFVPMAFFSGSTG-VIYRQFSITIVSAMTLSVLVALVLTPALCATLL--KPAAKG---HA 505 Query: 294 LFDTPFY-----------TRFRKLVTWCV--EWRKTTILLTLTAFMLGLLGFRFIEQQFF 340 L T F+ R++ +V + WR L A ++ GF + F Sbjct: 506 LATTGFFGWFNRQFDRSNIRYQGIVRHMLGRGWRYMAAYAVLLALVVA--GFMKLPVGFL 563 Query: 341 PDSSRPELMIELWLPEGSAFKATEAQAKKFEA-FIRKQ----DGVESVT--SYVGTG 390 PD + L + LP G+ T+ ++ E F+ Q GV +V+ S+ G+G Sbjct: 564 PDEDQGTLFAIMQLPSGATKARTDEVIRQVEQHFLVDQKEAVSGVFAVSGFSFAGSG 620 >gi|300310853|ref|YP_003774945.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1078 Score = 112 bits (281), Expect = 4e-26, Method: Compositional matrix adjust. Identities = 95/373 (25%), Positives = 176/373 (47%), Gaps = 26/373 (6%) Query: 31 NGTTFRLGDFAVVKREYVDPPVTKMRFNGKEAIGLGVSMEKGGDIIALGRNLDRMSDELR 90 NGT R+ D A V R+ V NGK ++ + ++ + G +II N+ + +L+ Sbjct: 250 NGTPVRVQDVAEV-RDSVADVRNAGSSNGKPSVLIIINRQPGANIIDTVDNVRELLPQLQ 308 Query: 91 TKLPVGIELERVSDQPKIVADSVGEFIHTLVEAVLIVLAVSFIALGLHTRPLRIDVRPGL 150 +P I+++ V D+ + S+ E TL+ +V +V+ V F+ L + R L Sbjct: 309 ASIPSAIDMKVVLDRTPTIRASLKETEFTLILSVALVIMVVFLFLR--------NARATL 360 Query: 151 VVALTIPLVLAVTFLFMRIFDIDLHKISLGALIIALGLLVDDAIIAVEMMVRKMEEGLSR 210 + A+ +P+ L TF M + L +SL AL IA G +VDDAI+ +E + R +E G+ Sbjct: 361 IPAVAVPVSLIGTFGVMYLAGFSLDNLSLMALTIATGFVVDDAIVVLENISRHIESGMKP 420 Query: 211 FDAATFAYTSTAIPMLTGTLITAAGFLPIGLAKSAAGEYTFSMFSVN-ALALLISWLVAV 269 + AA +L+ ++ A F+PI + + G F F++ ++A+L+S +V++ Sbjct: 421 YKAALLGAREVGFTVLSMSISLIAVFIPILMMEGIVGRL-FREFAITLSVAILVSLVVSL 479 Query: 270 VFTPYIGVLLLKVKPAAN--------------ADGHHELFDTPFYTRFRKLVTWCVEWRK 315 TP + LL+ PAA+ AD +F+ +++ + W + Sbjct: 480 TTTPMMCAQLLRHDPAADEARRQRRHPWLQKIADQSAAIFEDMLLA-YQRTLAWALRAAP 538 Query: 316 TTILLTLTAFMLGLLGFRFIEQQFFPDSSRPELMIELWLPEGSAFKATEAQAKKFEAFIR 375 +L+ L L + ++ I + FFP L+ + + +F+A + +F +R Sbjct: 539 LMMLILLATIALNVYLYKAIPKGFFPQQDTGRLVGGIRGDQAISFQAMRTKLNEFIDIVR 598 Query: 376 KQDGVESVTSYVG 388 V V ++ G Sbjct: 599 ADPAVSDVVAFTG 611 >gi|300313893|ref|YP_003777985.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1051 Score = 110 bits (274), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 112/411 (27%), Positives = 200/411 (48%), Gaps = 46/411 (11%) Query: 5 PGDNLQVRVSG--AFHSPKELEEMQLRA--NGTTFRLGDFAVVKREYVDPPVTKMRFNGK 60 PG + ++ +P E + +++ +G+ +L D A V+ V + NGK Sbjct: 226 PGQQINATITARSKLTTPDEFRNIIVKSSVDGSVVKLSDVARVELGGESYQVASQQ-NGK 284 Query: 61 EAIGLGVSMEKGGDIIALGRNLDRMSDELRTKLPVGIELER--VSDQPKIVADSVGEFIH 118 + L + + G + +A + + EL P ++L+ D V S+ E + Sbjct: 285 PSSALAIQLATGANALATADAVKKKLAELEPYFPASMKLKTNIAYDTTPFVRVSLEEVVK 344 Query: 119 TLVEAVLIVLAVSFIALGLHTRPLRIDVRPGLVVALTIPLVLAVTFLFMRIFDIDLHKIS 178 TL+EA+++V+ + ++ L ++R + A+T+P+VL TF + +F ++ ++ Sbjct: 345 TLIEAIVLVVLIMYLFLQ--------NIRATFIPAITVPVVLLGTFGILAMFGYSINTLT 396 Query: 179 LGALIIALGLLVDDAIIAVEMMVRKM-EEGLSRFDAATFAYTSTAIPMLTGTLIT----- 232 + +++A+GLLVDDAI+ VE + R M EE LS +A T ++ +TG L+ Sbjct: 397 MFGMVLAIGLLVDDAIVVVENVERLMSEEKLSPKEA-----TRKSMQEITGALVGIAMVL 451 Query: 233 AAGFLPIGLAKSAAG--EYTFSMFSVNALALLISWLVAVVFTPYIGVLLLKVKPAANADG 290 +A F+P+ + G FS+ V+A+AL S +VA+ TP + LLK +A G Sbjct: 452 SAVFIPMAFFGGSTGVIYRQFSITIVSAMAL--SVMVALTLTPALCATLLK----PHAGG 505 Query: 291 HHE------LFDTPF---YTRFRKLVTWCVEWRKTTILL-TLTAFMLGLLGFRFIEQQFF 340 H + F+ F TR++ V ++ K +L+ L A + +L F + F Sbjct: 506 HVQQGRFFTWFNRSFDRSATRYQGGVAGLLKRGKRGLLMYALIAVAMAVL-FMRLPTSFL 564 Query: 341 PDSSRPELMIELWLPEGSAFKATEAQAKKF-EAFIRKQDGVESVTSYVGTG 390 P+ + LM ++ LP G+ + T K E F+ K + VESV + VG G Sbjct: 565 PEEDQGVLMAQIQLPTGATAEQTATVVKTVQEYFMNKPESVESVLAIVGAG 615 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.141 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,289,015 Number of extensions: 50667 Number of successful extensions: 257 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 236 Number of HSP's successfully gapped: 10 Length of query: 453 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 365 Effective length of database: 1,203,190 Effective search space: 439164350 Effective search space used: 439164350 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 132 (55.5 bits)