BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0020.p (127 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311848|ref|YP_003775940.1| transmembrane protein [Herbaspi... 169 8e-44 >gi|300311848|ref|YP_003775940.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 131 Score = 169 bits (427), Expect = 8e-44, Method: Compositional matrix adjust. Identities = 86/127 (67%), Positives = 105/127 (82%), Gaps = 1/127 (0%) Query: 1 MIISKSAFTVGRVLLASLFVISGIFKIIGFSGTVGYMGSLGIPVPTVAVIVTILVEVGAG 60 MI S + +GRVLLASLFVISGIFKIIGF+GTVGY GSLG+P+PT+AV+ TILVEVG G Sbjct: 1 MITHYSTYRIGRVLLASLFVISGIFKIIGFAGTVGYFGSLGLPLPTLAVLATILVEVGGG 60 Query: 61 LLLLTGSRFARPAALLIAIFTVGATLAAHRFWAVDAAAMQGQLTHFLKNVSLIGGLLIVW 120 LLL+TG R RP +L+IA+FTVGATL+AH FW ++ AA QGQ+ HFLKNV+LIG LL+V Sbjct: 61 LLLMTG-RGVRPVSLVIALFTVGATLSAHHFWTLEGAAAQGQMIHFLKNVALIGALLLVS 119 Query: 121 SVKPADE 127 S+ P + Sbjct: 120 SIDPEAQ 126 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.142 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 311,594 Number of extensions: 10237 Number of successful extensions: 51 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 50 Number of HSP's successfully gapped: 1 Length of query: 127 Length of database: 1,619,870 Length adjustment: 75 Effective length of query: 52 Effective length of database: 1,264,745 Effective search space: 65766740 Effective search space used: 65766740 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 125 (52.8 bits)