BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0006.p (180 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314029|ref|YP_003778121.1| permease [Herbaspirillum serope... 205 1e-54 >gi|300314029|ref|YP_003778121.1| permease [Herbaspirillum seropedicae SmR1] Length = 310 Score = 205 bits (522), Expect = 1e-54, Method: Compositional matrix adjust. Identities = 101/126 (80%), Positives = 113/126 (89%) Query: 1 MNYTPGRLMAYVCLALSMSMVGAYVALTKPLAAALPVFLLAWLRFGIGAAAMLRWLKKPA 60 M+YTP R M Y CL LSMS+VGAYVALTKPLAA LPVFLLAWLRFGIGA AMLRWLKKPA Sbjct: 1 MSYTPSRPMGYGCLILSMSLVGAYVALTKPLAAMLPVFLLAWLRFGIGAVAMLRWLKKPA 60 Query: 61 NEAPLTPRTRLLVFLESFLGNFLFTICMISGVSMTSAVSAGVIMSVIPAVVALMSWIFLR 120 +E PL+ ++R LVFLESFLGNFLFT+CMI+GVSMT+AVSAGVIMS IPAVVA+MSWIFL+ Sbjct: 61 SEQPLSTQSRWLVFLESFLGNFLFTLCMITGVSMTNAVSAGVIMSAIPAVVAVMSWIFLK 120 Query: 121 ERIGAR 126 E+I R Sbjct: 121 EKISLR 126 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.137 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 424,693 Number of extensions: 14242 Number of successful extensions: 96 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 97 Number of HSP's successfully gapped: 2 Length of query: 180 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 101 Effective length of database: 1,245,805 Effective search space: 125826305 Effective search space used: 125826305 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 127 (53.5 bits)