BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0003.p (419 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311772|ref|YP_003775864.1| branched-chain amino acid ABC t... 679 0.0 gi|300310957|ref|YP_003775049.1| branched-chain amino acid ABC t... 341 5e-95 gi|300312236|ref|YP_003776328.1| amino acid ABC transporter peri... 92 8e-20 gi|300313931|ref|YP_003778023.1| branched-chain amino acid ABC t... 82 6e-17 gi|300310991|ref|YP_003775083.1| branched-chain amino acid ABC t... 65 7e-12 >gi|300311772|ref|YP_003775864.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 679 bits (1752), Expect = 0.0, Method: Compositional matrix adjust. Identities = 330/403 (81%), Positives = 368/403 (91%), Gaps = 2/403 (0%) Query: 17 FSFTYALQFAAVAAVLATAGLSGNAAAQATIKIGEINSYKAQPAFLEPYRQGWEMALEEI 76 F+F L++A AA+L ++ A AQ TI+IGEINSYKAQPAFLEPYR+GWEMAL+EI Sbjct: 4 FNFQRGLRWALAAALLTMGSVA--ARAQDTIRIGEINSYKAQPAFLEPYRRGWEMALDEI 61 Query: 77 NAAGGVLGKKLEVISRDDNGNPGDSVRVAEELVKREQVSLLFGGFLSNTGLALTDYARQR 136 NA+GGVLGKKLEVI+RDDNGNPGDSVRVAEEL+ RE+V+LLFGGFLSNTGLALTDYA+Q Sbjct: 62 NASGGVLGKKLEVIARDDNGNPGDSVRVAEELMARERVALLFGGFLSNTGLALTDYAKQH 121 Query: 137 KIFFLAAEPLTDKIVWHNGNKYTYRLRPSTYMLVASLVKDAAKLKKKRWAIVYPNYEYGQ 196 ++FFLAAEPLTDKIVW NGNKYTYRLRPST MLVAS+VK+A KLKKKRWAIVYPNYEYGQ Sbjct: 122 RVFFLAAEPLTDKIVWQNGNKYTYRLRPSTSMLVASVVKEAVKLKKKRWAIVYPNYEYGQ 181 Query: 197 SAVASFKTLMKAAQPDIEFVAEQAAPLGKIDAGAVTQALADAKPDAIFNVLFATDLGKFV 256 SAVASFKTLMK AQPD+EFVAEQA PLGKIDAGAVTQALADAKPDAIFN LFA DLGKFV Sbjct: 182 SAVASFKTLMKQAQPDVEFVAEQAPPLGKIDAGAVTQALADAKPDAIFNALFAADLGKFV 241 Query: 257 REGNTRGLFEGREVVSLLSGEPEYLDPLRAEAPSNWLVTGYPWYAINTPEHKKFVDAYRK 316 REGNTRGLFE +VVSLL+GEPEYLDPL+ EAP NW+VTGYPWYAI+TPEHKKFV+AYRK Sbjct: 242 REGNTRGLFEQVDVVSLLTGEPEYLDPLKGEAPQNWIVTGYPWYAIHTPEHKKFVEAYRK 301 Query: 317 KFNDYPRNGSLVGYSALMSIAAGLKKAGSADSDKLATAFSALRVETPVATITYRAQDHQS 376 KFNDYPRNGSLVGYSALMSIAAGL+KAGS D+D+LA AFS L+VETPVA+I YR QDHQS Sbjct: 302 KFNDYPRNGSLVGYSALMSIAAGLRKAGSTDADRLAAAFSGLKVETPVASIVYRPQDHQS 361 Query: 377 TLGAFVGRTGVKDGKGIMSSFVYVDGASLQPSDAEVKKLRAVD 419 TLGAFVGRTG+K+G+G+M+SF Y+DGA+LQ DAEVKKLRA D Sbjct: 362 TLGAFVGRTGLKEGRGVMTSFTYIDGAALQLPDAEVKKLRAPD 404 >gi|300310957|ref|YP_003775049.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 413 Score = 341 bits (875), Expect = 5e-95, Method: Compositional matrix adjust. Identities = 169/378 (44%), Positives = 246/378 (65%) Query: 39 GNAAAQATIKIGEINSYKAQPAFLEPYRQGWEMALEEINAAGGVLGKKLEVISRDDNGNP 98 G + A I+IGEINS + YR G +ALE+INAAGGV G+ L+++ R+D+G+ Sbjct: 30 GPSHAAEAIRIGEINSRHRLSTAGQAYRNGLALALEQINAAGGVGGRPLQLVVREDDGDA 89 Query: 99 GDSVRVAEELVKREQVSLLFGGFLSNTGLALTDYARQRKIFFLAAEPLTDKIVWHNGNKY 158 + R A L+ ++ V +L G LS+ LA++ A + + + + L+D I GN+Y Sbjct: 90 DQAARNARALISQDHVEVLTGSMLSDVALAVSREAARGRTVLVVGDALSDAITLDKGNRY 149 Query: 159 TYRLRPSTYMLVASLVKDAAKLKKKRWAIVYPNYEYGQSAVASFKTLMKAAQPDIEFVAE 218 T+R+RPSTYM A L + AA+L +RWA+V PNYEYGQSAVASFK L+K A+PD+EFV++ Sbjct: 150 TFRVRPSTYMQAAMLAEQAARLPGRRWAVVAPNYEYGQSAVASFKLLLKQARPDVEFVSD 209 Query: 219 QAAPLGKIDAGAVTQALADAKPDAIFNVLFATDLGKFVREGNTRGLFEGREVVSLLSGEP 278 Q LGKI A V +A+ ++PD IFN F DL KFV+ GN + LFEG VVS+ S Sbjct: 210 QWPALGKIKADDVARAIRQSRPDGIFNATFGEDLQKFVQAGNRQQLFEGVRVVSMASAGA 269 Query: 279 EYLDPLRAEAPSNWLVTGYPWYAINTPEHKKFVDAYRKKFNDYPRNGSLVGYSALMSIAA 338 LDP ++EA +WL GYPW ++ PEH++F++AY K+F++ P+ ++ GY+ +M+IAA Sbjct: 270 PSLDPSQSEAIRDWLAIGYPWDQVSLPEHQRFLEAYLKRFSERPQLSAVFGYTTMMAIAA 329 Query: 339 GLKKAGSADSDKLATAFSALRVETPVATITYRAQDHQSTLGAFVGRTGVKDGKGIMSSFV 398 GLKK+ D + L A L+ +P+ +++RA D Q++LGAFVG KDG+ M + Sbjct: 330 GLKKSEGGDGEVLVEAMRGLQFGSPLGPVSFRALDQQASLGAFVGLVSQKDGRSSMRDWR 389 Query: 399 YVDGASLQPSDAEVKKLR 416 Y+DGA+ P A V+ R Sbjct: 390 YLDGAAYLPPPAYVRSRR 407 >gi|300312236|ref|YP_003776328.1| amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 385 Score = 92.0 bits (227), Expect = 8e-20, Method: Compositional matrix adjust. Identities = 96/353 (27%), Positives = 154/353 (43%), Gaps = 17/353 (4%) Query: 28 VAAVLATAGLSGNAAAQATIKIGEINSYKAQPA-FLEPYRQGWEMALEEINAAGGVLGKK 86 + A+L A LS AA A IK+G + A + + G+ +A EEINA GGV G K Sbjct: 10 IPALLIGATLS-QAALAADIKLGVAEALTGPAAKYGVAIKNGFTLASEEINAKGGVNGDK 68 Query: 87 LEVISRDDNGNPGDSVRVAEELVKREQVSLLFGGFLSNTGLALTDYARQRKIFFLAAEPL 146 L ++ D+ G +++ V ++L+ +++V +FG LSN+ A A K+ Sbjct: 69 LALVIEDEQGKKEEAINVFKKLIFQDKVLAVFGPTLSNSAFAADPIANAAKVVVFGTSNT 128 Query: 147 TDKIVWHNGNKYTYRLRPSTYMLVASLVKDAAK-LKKKRWAIVYPNYE-YGQSAVASFKT 204 D I +T+R ++ VK A K K+ A++Y N + + +S FK Sbjct: 129 ADGITAM--GPFTFRNSVMEADVLPVTVKAAVKHFGIKKVAVIYGNDDAFTKSGYDVFKA 186 Query: 205 LMKAAQPDIEFVAEQAAPLGKIDAGAVTQALADAKPDAIFNVLFATDLGKFVREGNTRGL 264 ++ I +A G +D A + PDAI A + + + TR L Sbjct: 187 TLE--HQKIPVTTTEAYAKGDVDFKAQLTKIKAGNPDAIVCSCLAEEAANIILQ--TRAL 242 Query: 265 FEGREVVSLLSGEPEYLDPLRAEAPSNWLVTGYPWYAIN-TPEHKKFVDAYRKKFNDYPR 323 + + L + +A N L+ G PW A N TP +K F+ AY+ KF P Sbjct: 243 GMKQPFIGGNGLNSPKLFEIAKDAGDNTLM-GSPWSAENQTPANKAFITAYKAKFGADPD 301 Query: 324 NGSLVGYSALMSIAAGLKKAG-----SADSDKLATAFSALRVETPVATITYRA 371 + Y A+ +A LK + D D L A A++++ +RA Sbjct: 302 QFAAQAYDAMYIVADALKNVKLSGNLAKDRDALRAALPAVKIDGATGKFAFRA 354 >gi|300313931|ref|YP_003778023.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 397 Score = 82.4 bits (202), Expect = 6e-17, Method: Compositional matrix adjust. Identities = 81/314 (25%), Positives = 137/314 (43%), Gaps = 17/314 (5%) Query: 68 GWEMALEEINAAGGVLGKKLEVISRDDNGNPGDSVRVAEELVKREQVSLLFGGFLSNTGL 127 G ++AL++ G VLGK ++ D G P +VR +E +++ GG LS L Sbjct: 53 GMKLALQKY---GKVLGKSTSYVNLDTEGKPATAVRKMQESIEK-GTRFFAGGILSGEAL 108 Query: 128 ALTDYARQRKIFFLAAEPLTDKIVWHNGNKYTYRLRPSTYMLVASLVKDAAKLKK----K 183 A+ A + F+ D+I + N+ T+R T+ + V+ L+K K Sbjct: 109 AMGKEADKAGAVFITTAG-ADEITGSDCNRATFRWSVPTFGAIEQTVRPL--LEKMPNAK 165 Query: 184 RWAIVYPNYEYGQSAVASFKTLMKAAQPDIEFVAEQAAPLGKID-AGAVTQALADAKPDA 242 RW + P Y +G+ +++ K + K + IE V L + + +G +T A+A AKPD Sbjct: 166 RWYTITPQYVFGEGLLSAAKNIFK--EKGIEHVGNSYHSLTEKEFSGYLTNAIA-AKPDV 222 Query: 243 IFNVLFATDLGKFVREGNTRGLFEGREVVSLLSGEPEYLDPLRAEAPSNWLVTGYPWYAI 302 + + F + +R+ + G+ ++ + E + L A+ W+AI Sbjct: 223 LLLLNFGSQSSDTLRQAVSFGMKNNCTILMAWASGLEQFETLGADICDGVYFGAQYWHAI 282 Query: 303 NTPEHKKFVDAYRKKFNDYPRNGSLVG-YSALMSIAAGLKKAGSADSDKLATAFSALRVE 361 + P +K+FV P N SL G Y I + KA S D + A ++ E Sbjct: 283 DAPFNKEFVKLVNDNLKIDP-NYSLAGSYICTKIILDAIIKANSTDPKAVIAAMEGMKYE 341 Query: 362 TPVATITYRAQDHQ 375 + R DHQ Sbjct: 342 GLTGSEEIRKGDHQ 355 >gi|300310991|ref|YP_003775083.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 373 Score = 65.5 bits (158), Expect = 7e-12, Method: Compositional matrix adjust. Identities = 76/326 (23%), Positives = 133/326 (40%), Gaps = 28/326 (8%) Query: 66 RQGWEMALEEINAAGGVLGKKLEVISRDDNGNPGDSVRVAEELVKREQVSLLFGGFLSNT 125 + G A+E+INAAGG G K E + DD P +V VA ++V + + + G S + Sbjct: 49 KAGALTAIEQINAAGGANGNKFEAVMMDDACEPKQAVAVANKIV-SQGIKYVIGHVCSGS 107 Query: 126 GLALTDYARQRKIFFLAAEPLTDKIVWHNGNKYTYRL-------RPSTYMLVASLVKDAA 178 + +D + + ++ +K+ +R P+ V Sbjct: 108 TIPASDIYENEGVVMITPSATAPQLTEAKPHKFIFRTIGRDDQQGPAAARYVIE------ 161 Query: 179 KLKKKRWAIVYPNYEYGQSAVASFKTLMKAAQPDIEFVAEQAAPLGKIDAGAVTQALADA 238 KLK K+ A+++ YGQ +S K + AA+ + V + G D A+ L Sbjct: 162 KLKPKKVAVLHDKQSYGQGVASSVKAALDAAK--VPVVVFEGINAGDSDYSAIVTKLKSQ 219 Query: 239 KPDAIFNVLFATDLGKFVREGNTRGL---FEGREVVSLLSGEPEYLDPLRAEAPSNWLVT 295 D ++ + ++G +R+ +G+ F G E V + + + A LVT Sbjct: 220 GVDFVYFGGYHPEMGLIMRQAREQGIKAVFMGPEGVG-----NKDITAIAGPASEGMLVT 274 Query: 296 GYPWYAINTPEHKKFVDAYRKKFNDYPRNGSLVGYSALMSIAAGLKKAGSADSDKLATAF 355 +A + P + V A+ D + YS + IA + A S D +K+A Sbjct: 275 LPADFAAD-PANAALVKAFAAAKRDPNGPFQMPAYSGVKIIADAIAGAKSTDPEKVAAYI 333 Query: 356 SALRVETPVATITYRAQDHQSTLGAF 381 A +TP+ + Y D + L +F Sbjct: 334 HANSFKTPIGNVEY---DKKGDLKSF 356 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.133 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,260,560 Number of extensions: 53662 Number of successful extensions: 200 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 192 Number of HSP's successfully gapped: 18 Length of query: 419 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 331 Effective length of database: 1,203,190 Effective search space: 398255890 Effective search space used: 398255890 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)