BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0035.0043.p
         (137 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300312647|ref|YP_003776739.1| 2-keto-4-pentenoate hydratase [...    97   5e-22
gi|300311982|ref|YP_003776074.1| 2-keto-4-pentenoate hydratase [...    53   1e-08

>gi|300312647|ref|YP_003776739.1| 2-keto-4-pentenoate hydratase
           [Herbaspirillum seropedicae SmR1]
          Length = 247

 Score = 97.1 bits (240), Expect = 5e-22,   Method: Compositional matrix adjust.
 Identities = 58/132 (43%), Positives = 72/132 (54%), Gaps = 11/132 (8%)

Query: 9   PAIELVSSRYTEYPAANPLAGLADALSNCAFIYG-----PGRSDLGQIDQSIQHAELYFN 63
           PAIE+V +R    P  NP AG+AD  S+ A + G     P    L QI+     A L  +
Sbjct: 121 PAIEVVETRLGHPPCTNPWAGMADLQSHGALVTGAPQALPDLLSLRQIE-----ASLSID 175

Query: 64  GLEVARTVGGNPVVDIWPLVTWLVNHLCERCGGIKAGQFVTTGSCTGLLFADKGSSVNAL 123
           G +V+ T GGNP  D+WPL+ WL  H   R    +AGQ +TTGSCTGL  A  G  V A 
Sbjct: 176 GQQVSHTTGGNPA-DLWPLLGWLALHCARRGQPWRAGQIITTGSCTGLQMARGGRRVEAA 234

Query: 124 LQNIGKVDISFA 135
           L  IG V + FA
Sbjct: 235 LHGIGAVWLDFA 246


>gi|300311982|ref|YP_003776074.1| 2-keto-4-pentenoate hydratase
           [Herbaspirillum seropedicae SmR1]
          Length = 262

 Score = 52.8 bits (125), Expect = 1e-08,   Method: Compositional matrix adjust.
 Identities = 39/135 (28%), Positives = 58/135 (42%), Gaps = 1/135 (0%)

Query: 1   VAAVATVHPAIELVSSRYTEYPAANPLAGLADALSNCAFIYG-PGRSDLGQIDQSIQHAE 59
           + AV +VHP IE+  +RY ++        +AD      F+ G P   D   +D +     
Sbjct: 127 MEAVGSVHPGIEIPDTRYQQFREVGAYQLIADNCCADQFVLGAPTSMDWRHLDLAALATS 186

Query: 60  LYFNGLEVARTVGGNPVVDIWPLVTWLVNHLCERCGGIKAGQFVTTGSCTGLLFADKGSS 119
            +  G E     G   + D    +TWLVN L +    + AGQ V TGS    +    G +
Sbjct: 187 AFIEGGEQRSGSGAQVLGDPRIALTWLVNELRQHGLTLAAGQLVATGSTIVPMPVQAGQT 246

Query: 120 VNALLQNIGKVDISF 134
           V A L  +G V +  
Sbjct: 247 VRADLGVLGSVTVHL 261


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.320    0.136    0.416 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 453,445
Number of extensions: 18387
Number of successful extensions: 46
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 43
Number of HSP's successfully gapped: 2
Length of query: 137
Length of database: 1,619,870
Length adjustment: 76
Effective length of query: 61
Effective length of database: 1,260,010
Effective search space: 76860610
Effective search space used: 76860610
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 126 (53.1 bits)