BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0003.p (425 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309700|ref|YP_003773792.1| Mg2+/Co2+ transporter transmemb... 684 0.0 gi|300311018|ref|YP_003775110.1| CBS domains containing hemolysi... 110 2e-25 gi|300310418|ref|YP_003774510.1| hemolysin protein [Herbaspirill... 107 2e-24 gi|300310054|ref|YP_003774146.1| Mg2+ and Co2+ transporter prote... 100 3e-22 >gi|300309700|ref|YP_003773792.1| Mg2+/Co2+ transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 434 Score = 684 bits (1766), Expect = 0.0, Method: Compositional matrix adjust. Identities = 327/415 (78%), Positives = 378/415 (91%), Gaps = 1/415 (0%) Query: 1 VQILALALLILCSAFFSMTETALMAANRHRLRHLAKHGNKRAITLLWLLERTDRLLSLIL 60 VQ+LAL +LIL SAFFSMTETALMAANRHRLRHLAK GN+ AIT+LWLL+RT+RLLSL+L Sbjct: 8 VQVLALFILILLSAFFSMTETALMAANRHRLRHLAKQGNRAAITILWLLDRTERLLSLVL 67 Query: 61 IANTLINALATALVTALAITAFGNHEKVITIATACVAGLLIVFAEIVPKIIGATYPERIS 120 IANT INALATALVTALAI AFGNH++VIT ATACVA LLI+FAEIVPK+IGATYPERIS Sbjct: 68 IANTAINALATALVTALAIAAFGNHQRVITAATACVAALLIIFAEIVPKVIGATYPERIS 127 Query: 121 LFTGFFLKPLMALAKPLLWVVNIIVSTILRIIHIKASGRSHEQRLSPEELRSIVLEGGNF 180 LFTGF LKPLM +AKPL+W VN+ VS ILR++ IK R+ EQR+SPEELRSIVLEGG+F Sbjct: 128 LFTGFILKPLMRVAKPLIWFVNLFVSAILRLLRIKTGARAAEQRISPEELRSIVLEGGSF 187 Query: 181 IPQKHKSILLNLFDLEKISVEDVMTPRAQVEALNLSVSIDEIRNQLATCYHNKLPVYEGE 240 IPQKHKSILLNLFDLEKISVEDVMTPR+QVEALNL+ +D+I +QLATCYHNKLPV+EGE Sbjct: 188 IPQKHKSILLNLFDLEKISVEDVMTPRSQVEALNLAAPVDDILHQLATCYHNKLPVFEGE 247 Query: 241 INQIVGILHVRKAIMLFSQDEEITGEEIRALLSAPYFIPQDTDVFTQLQYFQENHERLGI 300 IN IVGILHVRK + LF ++EEI+ ++IR LLSAPY++P +TDVFTQLQYFQENHERLG+ Sbjct: 248 INHIVGILHVRKTVSLFRREEEISHDDIRELLSAPYYVPVETDVFTQLQYFQENHERLGV 307 Query: 301 IVDEYGEVQGLVTLEDIIEEMIGEFTTSVPGAARADSFAWNKDGECLLEGATTLRDINKR 360 +VDEYGEVQGLVTLEDIIEEMIGEFTTS PG++ + F W+KDG CLLEG+TTLRDINKR Sbjct: 308 VVDEYGEVQGLVTLEDIIEEMIGEFTTSSPGSS-GEGFGWDKDGACLLEGSTTLRDINKR 366 Query: 361 LGLSLPLDGPKTLNGLLLEWLRDIPEANVSVRIAGCIIEIVQVQNQAIKVVKLFA 415 LGL LPLDGPKT+NGLLLE+L+DIPEA+V+V++ GC+IEIVQVQNQAI++VKL A Sbjct: 367 LGLQLPLDGPKTINGLLLEYLQDIPEASVAVKMDGCVIEIVQVQNQAIRMVKLIA 421 >gi|300311018|ref|YP_003775110.1| CBS domains containing hemolysin protein [Herbaspirillum seropedicae SmR1] Length = 442 Score = 110 bits (275), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 104/424 (24%), Positives = 195/424 (45%), Gaps = 46/424 (10%) Query: 13 SAFFSMTETALMAANRHRLRHLAKHGNKRAITLLWLLERTDRLLSLILIANTLINALATA 72 SAF S E ++ A+ + RL ++K G A +L L + +++ I +NA+A Sbjct: 17 SAFLSCAEISIAASRKIRLEMMSKEGVHNADRVLSLQAQPGNFFTVVQIG---LNAVAI- 72 Query: 73 LVTALAITAFGNHEKVI---------------TIATACVAGLLIVFAEIVPKIIGATYPE 117 L + A H + I ++ V I+FA+++PK + PE Sbjct: 73 LGGVVGEPALTPHMREIISSFYIGPWLENASFAMSFLVVTAFFILFADLMPKRLAMVAPE 132 Query: 118 RISLFTGFFLKPLM---ALAKPLLWVVNIIVSTILRIIHIKASGRSHEQRLSPEELRSIV 174 R ++ +KP++ L KPL+W N + I + + + ++ ++P E+ ++V Sbjct: 133 RAAVA---LVKPMLLLETLFKPLVWFFNSLSKWIFTLFGLP---QVRQEVITPNEIHAMV 186 Query: 175 LEG---GNFIPQKHKSILLNLFDLEKISVEDVMTPRAQVEALNLSVSIDEIRNQLATCYH 231 G G + Q+H ++ N+F+LE V MTPR + L+ S IR ++ H Sbjct: 187 DAGAQAGVLLTQEHH-LIENVFELESRYVPSAMTPRDSIVYFLLTDSEATIRQKIIDHPH 245 Query: 232 NKLPVYEGEINQIVGILHVRKAIMLFSQDEEITGEEIRALLSAPYFIPQDTDVFTQLQYF 291 ++ V +G I+ ++G + + + + E+ + + + L+ P IP +++ L+ Sbjct: 246 SRFLVCDGHIDAVLGYIDSKDVLKRLLKGEQFSLAD-KGLVHPPLSIPDSLNLWEVLERM 304 Query: 292 QENHERLGIIVDEYGEVQGLVTLEDIIEEMIGEF---TTSVPGAARADSFAWNKDGECLL 348 + E L I+V+EY V G+++ D+ ++G T R D +W L+ Sbjct: 305 KAMDEDLAIVVNEYALVVGIISTTDVTGVLMGNLLAITEEEQQIVRRDENSW------LM 358 Query: 349 EGATTLRDINKRLGLSL---PLDGPKTLNGLLLEWLRDIPEANVSVRIAGCIIEIVQVQN 405 +G T L D+ L + P+ G +TL+G ++ LR IP+ V A E+V ++ Sbjct: 359 DGLTPLNDVMSELDIDEYPNPV-GYETLSGFIMYMLRKIPKKTDFVLFAEFKFEVVDIEG 417 Query: 406 QAIK 409 I Sbjct: 418 NRIN 421 >gi|300310418|ref|YP_003774510.1| hemolysin protein [Herbaspirillum seropedicae SmR1] Length = 440 Score = 107 bits (267), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 97/429 (22%), Positives = 189/429 (44%), Gaps = 30/429 (6%) Query: 8 LLILCSAFFSMTETALMAANRHRLRHLAKHGNKRAITLLWLLERTDRLLSLILIANTLIN 67 L+ + FF E +L+ + R+R +AK R L + + D LS + TL + Sbjct: 12 FLVALNGFFVAAEFSLVKLRQTRIRAIAKTQGMRGRILAVVHNQLDAYLSACQLGITLAS 71 Query: 68 ---------ALATALVTALAITAFGNHEKV----ITIATACVAGLLIVFAEIVPKIIGAT 114 A A L ++ N E + A ++ L IV E+ PK + Sbjct: 72 LGLGWIGEPAFARILEPLFSLAGVTNQELIHGVSFVFAFFVISFLHIVAGELAPKSMAIR 131 Query: 115 YPERISLFTGFFLKPLMALAKPLLWVVNIIVSTILRIIHIKASGRSHEQRLSPEELRSIV 174 PE++ L+ L PL+WV+N + +LR+ + A G H+ S +EL+ ++ Sbjct: 132 SPEKLGLWCAMPLYGFYWGMYPLIWVLNASSNWLLRVAGLGA-GHGHDAHYSSDELK-LI 189 Query: 175 LEGGNFIPQKHK------SILLNLFDLEKISVEDVMTPRAQVEALNLSVSIDEIRNQLAT 228 L GN + K ++L + ++ V D+M P +++ AL S++E + + Sbjct: 190 LRAGNKSGKNGKFTRDEWNVLTQSLNFAELDVADIMRPASEIVALGDDKSLEENLDIIYR 249 Query: 229 CYHNKLPVYEGEINQIVGILHVRKAIMLFSQDEEITGEEIRALLSAPYFIPQDTDVFTQL 288 +++ P Y+ E Q++G++H+ K + L QD ++ L +I L Sbjct: 250 NRYSRYPYYDAERQQVLGLVHL-KDVFLAQQDGRAIA-NLKDYLRPVQYISPALPALDLL 307 Query: 289 QYFQENHERLGIIVDEYGEVQGLVTLEDIIEEMIGEFTTSVPGAARADSFAWNK--DGEC 346 + F+ +I + G +TL++++ ++GE R ++ W + DG Sbjct: 308 RRFRTGSPHFAVIGKKGQPPAGFITLDNMLSLLVGEIRDEF----RHNTGEWTRQDDGTL 363 Query: 347 LLEGATTLRDINKRLGLSLPLDGP-KTLNGLLLEWLRDIPEANVSVRIAGCIIEIVQVQN 405 L +G+ + + LG+ + D ++ GL++E L D+P+ + + I ++ Sbjct: 364 LGKGSLPIVTLENMLGIDIEYDDSIDSVGGLVMEKLGDLPKEGQKIDFPAFDVVIKRMSG 423 Query: 406 QAIKVVKLF 414 I +VK++ Sbjct: 424 PKIVLVKVY 432 >gi|300310054|ref|YP_003774146.1| Mg2+ and Co2+ transporter protein [Herbaspirillum seropedicae SmR1] Length = 292 Score = 100 bits (248), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 59/237 (24%), Positives = 120/237 (50%), Gaps = 8/237 (3%) Query: 179 NFIPQKHKSILLNLFDLEKISVEDVMTPRAQVEALNLSVSIDEIRNQLATCYHNKLPVYE 238 N I S++ +F + ++V D+M PR+Q++ +++S I+ ++ H++ P + Sbjct: 47 NLIDADALSMIEGVFQVSDLAVRDIMVPRSQMDTIDISKPIETWMPEVLATSHSRFPAVD 106 Query: 239 GEINQIVGILHVRKAIMLFSQDEEITGEEIRALLSAPYFIPQDTDVFTQLQYFQENHERL 298 GE +++VGIL + K ++ + +E ++R +L FIP+ + L+ F+ N + Sbjct: 107 GERDKVVGIL-LAKDLLRYYAEESF---DVRDMLRPAIFIPESKRLNVLLRDFRANRNHM 162 Query: 299 GIIVDEYGEVQGLVTLEDIIEEMIGEFTTSVPGAARADSFAWNKDG----ECLLEGATTL 354 ++VDEYG V GL+T+ED++E+++G+ D+ KDG ++ T + Sbjct: 163 AVVVDEYGGVAGLITIEDVLEQIVGDIEDEYDFDEAEDNVLALKDGPHGARWRVKALTEI 222 Query: 355 RDINKRLGLSLPLDGPKTLNGLLLEWLRDIPEANVSVRIAGCIIEIVQVQNQAIKVV 411 N+ +G D T+ GL+ + +P I G E+++ + + V+ Sbjct: 223 EQFNEEVGSRFSDDDADTIGGLVANHVGRVPRKGDEFDIDGLHFEVLRADARQVHVL 279 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.138 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,063,116 Number of extensions: 39114 Number of successful extensions: 140 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 130 Number of HSP's successfully gapped: 4 Length of query: 425 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 337 Effective length of database: 1,203,190 Effective search space: 405475030 Effective search space used: 405475030 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)