BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0034.0459.p
         (356 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300313307|ref|YP_003777399.1| gluconolactonase [Herbaspirillu...   262   3e-71
gi|300313737|ref|YP_003777829.1| gluconolactonase [Herbaspirillu...    72   5e-14

>gi|300313307|ref|YP_003777399.1| gluconolactonase [Herbaspirillum
           seropedicae SmR1]
          Length = 305

 Score =  262 bits (670), Expect = 3e-71,   Method: Compositional matrix adjust.
 Identities = 141/315 (44%), Positives = 190/315 (60%), Gaps = 22/315 (6%)

Query: 47  PDPNVQVLDNSFLKYRLYSSTVEQLATGFRWVEGPVWFGDGRYLLFSDIPNNRIMRWDEV 106
           P+   Q  D  F +  + ++ V+ L TG RW EGPVW    + L++SDIPNNR+MRW E 
Sbjct: 4   PETPFQHFDARFRRMVIDTAQVDCLYTGCRWAEGPVWLPATQELIWSDIPNNRLMRWSEG 63

Query: 107 TGTTSVFRNPANFTNGLARDRQGRLIACEHLTRRITRTEYDGRITVLADNYNGKRFNSPN 166
            G   VFR P+NF NG   DR+GR++ C H  R + RTE+DG IT LA ++ GKR NSPN
Sbjct: 64  AGA-GVFRQPSNFNNGNTLDREGRIVGCLHGGRAVVRTEHDGSITTLASHWQGKRLNSPN 122

Query: 167 DIICKSDGSIWFTDPPFGIAGEWEGDKQASELPHA-VYRIDGQSGKLSLITDALAGPNGL 225
           D++ KSDGSIWFTDP +GI  ++EG +  SE+    VYRIDG SG++S++   L  PNGL
Sbjct: 123 DVVVKSDGSIWFTDPDYGINSDYEGYQAQSEIGACNVYRIDGDSGEISIVASDLERPNGL 182

Query: 226 AFSPDEKVIYIVES----RATPNRLIWAYQLTDN-GTKLGSRTLAVDAAGPGAIDGFKVD 280
           AFS DE+ +YI ++    RA     I  + + D    K+G     +D   PG  DGF++D
Sbjct: 183 AFSTDERRLYIADTGLTHRAGGPHHIRVFDVVDGISLKVGEVFATID---PGLADGFRLD 239

Query: 281 VDGNLWCGWGSNGALDAKPAELDGVRVFTPQGKAIGHIHLPERCPNLVFGGAKKNRLFMA 340
             GN+W   G            DGV  + P G  +G I +PE   N+VFGG + NRLF+ 
Sbjct: 240 AQGNVWTSAG------------DGVHCYAPDGTLLGKILIPEVVSNVVFGGPRGNRLFIT 287

Query: 341 SSHSLYAVYVDTRGA 355
           ++ SLYAVY+   GA
Sbjct: 288 ATTSLYAVYLAVNGA 302


>gi|300313737|ref|YP_003777829.1| gluconolactonase [Herbaspirillum
           seropedicae SmR1]
          Length = 306

 Score = 72.4 bits (176), Expect = 5e-14,   Method: Compositional matrix adjust.
 Identities = 69/271 (25%), Positives = 112/271 (41%), Gaps = 29/271 (10%)

Query: 77  WVEGPVWFGDGRYLLFSDIPNNRIMRWDEVTGTTSVFRNPANFTNGLARDRQGRLIACEH 136
           ++EGP +   GR L  +DIP  RI R   + G   +      + NGL  D+ GR++  ++
Sbjct: 43  FLEGPSFDRQGR-LYVTDIPYGRIFRI-TMDGQWELVTEYDGWPNGLKIDKTGRIVITDY 100

Query: 137 LTRRITRTEYDGRITVLADNYNGKRFNSPNDIICKSDGSIWFTDPPFGIAGEWEGDKQAS 196
               +      G +T L +    + F   ND++    G I+FTD         +G     
Sbjct: 101 KRGLVQLDPDTGTVTPLLETVGSEGFKGVNDLVFGPKGEIYFTD---------QGQTGLQ 151

Query: 197 ELPHAVYRIDGQSGKLSLITDALAGPNGLAFSPDEKVIYIVESRATPNRLIWAYQLTDNG 256
           +    VYR+    G+L+ + + +  PNG+ + P    + +  +RA   + IW   L  NG
Sbjct: 152 DPTGRVYRL-APDGQLTCLINTIPSPNGIVYDPALNHLLVAVTRA---QQIWRIPLHHNG 207

Query: 257 TKLGSRTLAVDAAGPGAIDGFKVDVDGNLWC---GWGSNGALDAKPAELDGVRVFTPQGK 313
                   A    G    DG  +D + NL+    G+GS   L      L  +R+ +  G 
Sbjct: 208 VVGKVGVFANLHGGMAGPDGLALDAESNLYIAHTGFGSIWRLSKFAEPL--LRIRSSAGI 265

Query: 314 AIGHIHLPERCPNLVFGGAKKNRLFMASSHS 344
           +           NL FGG     LF+  S +
Sbjct: 266 S---------TTNLAFGGPDGKSLFITESQT 287


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.319    0.136    0.423 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,181,114
Number of extensions: 52961
Number of successful extensions: 125
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 121
Number of HSP's successfully gapped: 2
Length of query: 356
Length of database: 1,619,870
Length adjustment: 86
Effective length of query: 270
Effective length of database: 1,212,660
Effective search space: 327418200
Effective search space used: 327418200
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 131 (55.1 bits)