BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0458.p (123 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311869|ref|YP_003775961.1| short-chain alcohol dehydrogena... 94 4e-21 gi|300312612|ref|YP_003776704.1| 3-ketoacyl-CoA reductase [Herba... 75 2e-15 gi|300311122|ref|YP_003775214.1| short-chain dehydrogenase [Herb... 71 3e-14 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 71 3e-14 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 70 7e-14 >gi|300311869|ref|YP_003775961.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 257 Score = 93.6 bits (231), Expect = 4e-21, Method: Compositional matrix adjust. Identities = 54/119 (45%), Positives = 68/119 (57%), Gaps = 5/119 (4%) Query: 8 LQQGGRIVNFSTSVIGLALPGYAVYAATKGAIEVMTNIFAKELRGKQITVNAVAPGPTAT 67 L QGGRI+N S+ + +LPGYA YA+ KG +EVMT AKE + I VN VAPG T Sbjct: 138 LAQGGRIINISSGLARFSLPGYAAYASMKGGVEVMTRYMAKEFGARGIVVNTVAPGAIET 197 Query: 68 ALFLNGKTPDVVERMSKAA---PLERLGTPEDIAKAVTYLAAPD-GWVNGQTLRANGGL 122 F G+ D E S A L R G P+DI A+ L PD GW+N Q + +GG+ Sbjct: 198 D-FGGGRVRDNAELNSFVAHQTALGRAGLPDDIGAAIASLLRPDNGWINAQRVEVSGGM 255 >gi|300312612|ref|YP_003776704.1| 3-ketoacyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 253 Score = 74.7 bits (182), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 48/117 (41%), Positives = 64/117 (54%), Gaps = 5/117 (4%) Query: 9 QQGGRIVNFSTSVIGL--ALPGYAVYAATKGAIEVMTNIFAKELRGKQITVNAVAPGPTA 66 Q GG I+N TSV PG +YAA+KG + +T AKEL +I VNAVAPG Sbjct: 134 QGGGNIINV-TSVAARHGGGPGALIYAASKGFVSTLTRGMAKELLADKIRVNAVAPGVIM 192 Query: 67 TALFLNGKTPDVVERMSKAAPLERLGTPEDIAKAVTYLAAP--DGWVNGQTLRANGG 121 T TP+ +E K P+ R+G P++ + A YLA+ G+V GQ + NGG Sbjct: 193 TPFQERFTTPEQLEGFRKTIPMGRIGEPDECSGAFLYLASEQMSGYVTGQIIDVNGG 249 >gi|300311122|ref|YP_003775214.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 70.9 bits (172), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 45/122 (36%), Positives = 67/122 (54%), Gaps = 2/122 (1%) Query: 2 RAAAAHLQQGGRIVNFSTSVIGLALPG-YAVYAATKGAIEVMTNIFAKELRGKQITVNAV 60 R + H QGG IVN S+ L PG Y YAA+KGAI+ T AKE+ + I VNAV Sbjct: 129 RMSTRHGGQGGAIVNLSSMAAKLGGPGEYVDYAASKGAIDAFTIGLAKEVAAEGIRVNAV 188 Query: 61 APGPTATALFLNGKTPDVVERMSKAAPLERLGTPEDIAKAVTYLAAPD-GWVNGQTLRAN 119 PG T + +G V+R+ A P++R G+ ++A A+ +L + + + G + + Sbjct: 189 RPGLIYTDIHASGGEAGRVDRLKDAVPMKRGGSAAEVANAILWLLSEEASYATGTFIDVS 248 Query: 120 GG 121 GG Sbjct: 249 GG 250 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 70.9 bits (172), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 48/118 (40%), Positives = 64/118 (54%), Gaps = 14/118 (11%) Query: 12 GRIVNFSTSVIGLA-LPGYAVYAATKGAIEVMTNIFAKELRGKQITVNAVAPGPTATALF 70 GRI+N TSV+G A PG YAA K + M+ A+E+ + ITVN VAPG F Sbjct: 138 GRIINI-TSVVGEAGNPGQMNYAAAKAGVAGMSRALAREIGSRNITVNCVAPG------F 190 Query: 71 LNGKTPDVVERMSKAA-----PLERLGTPEDIAKAVTYLAAPD-GWVNGQTLRANGGL 122 ++ + AA PL RLG E+IA AV +LA+P ++ G TL NGG+ Sbjct: 191 IDTDMTRALNEQQSAAILQQIPLGRLGAAEEIAYAVAFLASPQAAYITGTTLNVNGGM 248 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 69.7 bits (169), Expect = 7e-14, Method: Compositional matrix adjust. Identities = 46/117 (39%), Positives = 63/117 (53%), Gaps = 3/117 (2%) Query: 9 QQGGRIVNFSTSVIGL-ALPGYAVYAATKGAIEVMTNIFAKELRGKQITVNAVAPGPTAT 67 QQ GRIVN S S+ G+ A G Y +K A+ +T A EL ITVN+VAPGP T Sbjct: 134 QQWGRIVNIS-SISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQYGITVNSVAPGPVDT 192 Query: 68 ALFLNGKTPDVVERMSKAAPLERLGTPEDIAKAVTYLAAPDG-WVNGQTLRANGGLV 123 + + E + P+ R GT ++IA AV YLAA + ++ G + +GG V Sbjct: 193 PMTQAMHSEQTRESYYRLVPMRRYGTTDEIASAVCYLAAEESAYITGHVIPVDGGFV 249 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.132 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 432,944 Number of extensions: 16846 Number of successful extensions: 138 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 136 Number of HSP's successfully gapped: 17 Length of query: 123 Length of database: 1,619,870 Length adjustment: 74 Effective length of query: 49 Effective length of database: 1,269,480 Effective search space: 62204520 Effective search space used: 62204520 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 125 (52.8 bits)