BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0453.p (344 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313794|ref|YP_003777886.1| ferrichrome-iron receptor prote... 176 3e-45 gi|300311660|ref|YP_003775752.1| TonB-dependent receptor protein... 159 4e-40 gi|300311652|ref|YP_003775744.1| TonB-dependent siderophore rece... 145 6e-36 gi|300310824|ref|YP_003774916.1| hydroxamate-type ferrisideropho... 142 3e-35 gi|300309529|ref|YP_003773621.1| TonB dependent ferrisiderophore... 115 7e-27 >gi|300313794|ref|YP_003777886.1| ferrichrome-iron receptor protein [Herbaspirillum seropedicae SmR1] Length = 817 Score = 176 bits (445), Expect = 3e-45, Method: Compositional matrix adjust. Identities = 114/260 (43%), Positives = 157/260 (60%), Gaps = 19/260 (7%) Query: 23 GAPLISQAQSAEPAGAT------ALPAVTVSAVRDADSPTGPGNG-YVAKRSLAGTKTDT 75 G L+ +++A P+ AT +LPA+ +SA D PG + A+ S + TKT T Sbjct: 108 GYVLVPASEAATPSAATTPAASGSLPAIQISASAD------PGEQRWTARDSASTTKTAT 161 Query: 76 PLVETPQSISVVTREQMDAQAAQTLDAALRYTPGVYSQD--NDLRFDQLSARGFDLDS-- 131 PL+E PQSISVVT E++ A A T AL YTPGV +D R+D ++ RGFD S Sbjct: 162 PLIEVPQSISVVTAERIAAMGATTARDALGYTPGVNISPYGSDSRYDWINLRGFDAYSPG 221 Query: 132 -YLDGLKLVKTATWFVTPRIDTSLLDRVEVLHGPASVLYGQAAPGGVVNMVSKRPTEEAF 190 YLDGL L + A F R + ++++ VL GPASVLYGQA PGGVVN+VSK P+ +A Sbjct: 222 FYLDGLPL-RNANSFAVWRTENYGIEQLAVLRGPASVLYGQAGPGGVVNVVSKLPSADAP 280 Query: 191 HRIDFQAGNNDRYQVGFDMGGPVDADGKVLYRVTGLGRYAGTQTDHVREERYAIAPSVTI 250 + Q G++ R QVG D+GG +DADG++LYRV G+ R A ++ +R +APS+ Sbjct: 281 RELQVQLGSDRRRQVGADIGGAIDADGRLLYRVIGVVRDAELPAGGMKNDRLYLAPSLVW 340 Query: 251 KPGADTTLTILTSLQHDPAG 270 K A T+LT+ +L D G Sbjct: 341 KISAATSLTLQAALLRDQGG 360 >gi|300311660|ref|YP_003775752.1| TonB-dependent receptor protein [Herbaspirillum seropedicae SmR1] Length = 705 Score = 159 bits (401), Expect = 4e-40, Method: Compositional matrix adjust. Identities = 104/273 (38%), Positives = 154/273 (56%), Gaps = 27/273 (9%) Query: 10 FSAIQLATMTLCLGAPLISQAQSAEPAGATALPAVTVSAVRDADSPTGPGNGYVAKRSLA 69 F A+Q A +TL + A ALP V + A DA++ GP NGYVAKR+ + Sbjct: 10 FLAVQCALLTL----------AAPAWAQQQALPQVEIKA--DAETANGPVNGYVAKRTAS 57 Query: 70 GTKTDTPLVETPQSISVVTREQMDAQAAQTLDAALRYTPGVYSQDNDLR---FDQLSARG 126 TKTDTP++E PQS+SVVTR++MDA+ +L LRY PG S+ + + ++ + RG Sbjct: 58 ATKTDTPVLEVPQSVSVVTRDEMDARGVTSLLDVLRYMPGANSETHGVDPRGYEYFNLRG 117 Query: 127 FD----LDSYLDGLKLVKTATWFVTPRIDTSLLDRVEVLHGPASVLYGQAAPGGVVNMVS 182 F ++L+GL+ + F R +T ++R+EVL GP SV +GQA PGGV+N VS Sbjct: 118 FINAQVTSNFLNGLRQIPGG--FGQFRTETYGMERIEVLRGPGSVTFGQADPGGVINRVS 175 Query: 183 KRPTEEAFHRIDFQAGNNDRYQVGFDMGGPVDADGKVLYRVTGLGRYAGTQTDHVR---- 238 K P + + + G+ +R QV D+ G DG+ L+RV GLG + Q Sbjct: 176 KLPGMDIPNELGVDIGSFNRKQVVADLNGHTQ-DGEYLWRVVGLGLDSNNQYKFNNGQSG 234 Query: 239 -EERYAIAPSVTIKPGADTTLTILTSLQHDPAG 270 R+ +APS+T +P A T+LT++ +D +G Sbjct: 235 DNNRFYLAPSLTWRPSASTSLTLMADYMNDRSG 267 >gi|300311652|ref|YP_003775744.1| TonB-dependent siderophore receptor protein [Herbaspirillum seropedicae SmR1] Length = 832 Score = 145 bits (365), Expect = 6e-36, Method: Compositional matrix adjust. Identities = 94/239 (39%), Positives = 132/239 (55%), Gaps = 14/239 (5%) Query: 44 VTVSAVRDADSPTGPGNGYVAKRSLAGTKTDTPLVETPQSISVVTREQMDAQAAQTLDAA 103 +TVS RD P GYVA RS TKTDTPL+E QS+SVVTR +M+A+ A ++ Sbjct: 159 ITVSGRRDEPGAADPVEGYVALRSATATKTDTPLLELAQSVSVVTRAEMEARGANSILDV 218 Query: 104 LRYTPGVYSQDNDLR---FDQLSARGF----DLDSYLDGLKLVKTATWFVTPRIDTSLLD 156 LRY PG ++ + + +D + RGF +YL+GL+ + + F R +T L+ Sbjct: 219 LRYMPGANTETHGVDPRGYDYFNLRGFINAQTTSNYLNGLRQIPSG--FGMFRTETYGLE 276 Query: 157 RVEVLHGPASVLYGQAAPGGVVNMVSKRPTEEAFHRIDFQAGNNDRYQVGFDMGGPVDAD 216 RVEVL G S +GQA PGGVVN VSK A + + G+ R QV D+ G +D + Sbjct: 277 RVEVLRGANSASFGQADPGGVVNRVSKLAGSGARNELMVDVGSFQRRQVAADLSGDLDPE 336 Query: 217 GKVLYRVTGLGRYAGTQTDHVR-----EERYAIAPSVTIKPGADTTLTILTSLQHDPAG 270 G+V R+ GL TQ + +R +APS+ I+P ADT+L +L D +G Sbjct: 337 GRVQARLVGLLLDGDTQFRYANGRAGDNDRLYLAPSLKIRPSADTSLILLAEYLKDRSG 395 >gi|300310824|ref|YP_003774916.1| hydroxamate-type ferrisiderophore receptor signal peptide protein, tonB-dependent receptor [Herbaspirillum seropedicae SmR1] Length = 715 Score = 142 bits (359), Expect = 3e-35, Method: Compositional matrix adjust. Identities = 105/250 (42%), Positives = 134/250 (53%), Gaps = 26/250 (10%) Query: 30 AQSAEPAGATA--------LPAVTVSAVRDADSPTGPGNGYVAKRSLAGTKTDTPLVETP 81 AQ+A P GA + LP V+V+A DS V K S TPL ETP Sbjct: 29 AQTAPPQGADSSSTAASAALPTVSVTADGYTDSKFKATQTQVNKSS-------TPLSETP 81 Query: 82 QSISVVTREQMDAQAAQTLDAALRYTPGVYSQDNDLR-FDQLSARG-FDLDS-YLDGLKL 138 QSISVVTR MD+Q A +L AL+ GV +Q R +D L RG DS YLDGL+ Sbjct: 82 QSISVVTRAMMDSQQAVSLSDALKNVSGVVAQQYGRRGWDDLIIRGQVASDSLYLDGLRT 141 Query: 139 VKTATWFVTPRIDTSL--LDRVEVLHGPASVLYGQAAPGGVVNMVSKRPTEEAFHRIDFQ 196 ++ R+ L L +VEVL GP S+LYG PGG+VNMVSKRP F +D Sbjct: 142 TASS------RVAEQLYGLQQVEVLKGPGSLLYGLVLPGGLVNMVSKRPQGSDFANVDVT 195 Query: 197 AGNNDRYQVGFDMGGPVDADGKVLYRVTGLGRYAGTQTDHVREERYAIAPSVTIKPGADT 256 G++D YQ D+ + +GK +R+ GL + TDHV + IAPS+++ GA T Sbjct: 196 IGSHDLYQSTVDLNKSLSENGKQAFRINGLISNSDDATDHVWFKNRYIAPSLSLDLGART 255 Query: 257 TLTILTSLQH 266 TILTS Q Sbjct: 256 DFTILTSYQE 265 >gi|300309529|ref|YP_003773621.1| TonB dependent ferrisiderophore receptor protein [Herbaspirillum seropedicae SmR1] Length = 704 Score = 115 bits (287), Expect = 7e-27, Method: Compositional matrix adjust. Identities = 89/268 (33%), Positives = 140/268 (52%), Gaps = 27/268 (10%) Query: 6 MVFGFSAIQLATMTLCLGAPLISQAQSAEPAGATALPAVTVSAVRDADSPTGPGNGYVAK 65 M F S ++LA + C +PL A+ TALPA+ VS R D GYVA Sbjct: 1 MNFRLSPLRLAVLATCSLSPLAILPACAQ-QDTTALPAIEVSGARAND-------GYVAP 52 Query: 66 RSLAGTKTDTPLVETPQSISVVTREQMDAQAAQTLDAALRYTPGVYSQDNDLRFDQLSAR 125 +++GTK++ PL + PQ+I+VV + + Q A +L L+ PG+ D + DQ+ R Sbjct: 53 TAVSGTKSEAPLRDVPQTINVVPAQVIRDQNASSLQDVLKNVPGLGLSTGDGQRDQVFIR 112 Query: 126 GFDL--DSYLDGLKLVKTATWFVTPRIDTSLLDRVEVLHGPASVLYGQAAPGGVVNMVSK 183 GF D ++DG + A +F D S ++ VEV+ GPA+VLYG+ + GG++N V+K Sbjct: 113 GFTAIGDQFVDGFR--DDALYFR----DLSNVESVEVIKGPAAVLYGRGSSGGLINRVTK 166 Query: 184 RPTEEAFHRIDFQAGNNDRYQVGFDMGGPVDADGKVLYRVTGLGRYAGTQTDHVREERY- 242 +P + + F A + + D+G +G + +R+TG A + D R +++ Sbjct: 167 KPGVDV-SDVSFTANSTAGRRAELDLGRA--GEGALSWRLTG----AVERGDSFRAQQFI 219 Query: 243 ---AIAPSVTIKPGADTTLTILTSLQHD 267 AIAPSV ++ ADT L + D Sbjct: 220 DRTAIAPSVALRFSADTKLLLQMDYLED 247 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.133 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,110,964 Number of extensions: 46767 Number of successful extensions: 191 Number of sequences better than 1.0e-08: 16 Number of HSP's gapped: 170 Number of HSP's successfully gapped: 16 Length of query: 344 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 258 Effective length of database: 1,212,660 Effective search space: 312866280 Effective search space used: 312866280 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)