BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0452.p (393 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311660|ref|YP_003775752.1| TonB-dependent receptor protein... 233 1e-62 gi|300311652|ref|YP_003775744.1| TonB-dependent siderophore rece... 219 2e-58 gi|300313794|ref|YP_003777886.1| ferrichrome-iron receptor prote... 201 9e-53 gi|300310859|ref|YP_003774951.1| TonB-dependent siderophore rece... 155 5e-39 gi|300310824|ref|YP_003774916.1| hydroxamate-type ferrisideropho... 108 5e-25 >gi|300311660|ref|YP_003775752.1| TonB-dependent receptor protein [Herbaspirillum seropedicae SmR1] Length = 705 Score = 233 bits (595), Expect = 1e-62, Method: Compositional matrix adjust. Identities = 145/359 (40%), Positives = 197/359 (54%), Gaps = 8/359 (2%) Query: 40 HLTNFAVDNQAQIKFNTGALAHTTLVGIDYQRTLGGVNRGGAVTGSINIYNPNFAALSTA 99 + N VDNQ + + GA HT L G+D+ R R S+NI NP + A Sbjct: 350 QVDNTLVDNQLEGRLKWGATEHTVLFGLDWSRMYDREVRYRGAAPSLNIDNPVYGVTIPA 409 Query: 100 QITTFEDY--SLTQVGAYLQDQVKLGN-WSLQLGGRKDWTTSNDVQRTGAVTTVARQNDS 156 T F + ++ Q G Y QDQ+K +L LGGR W D R T R +DS Sbjct: 410 ATTLFGNLEENMNQTGLYAQDQIKFDQRLTLTLGGR--WDKVKDETRNYFNRTTTRSDDS 467 Query: 157 AFTWRGGLTYQFDSGLAPYVSYAKSFQPTIGSNTGAAPFVPTTGKQYEAGVKFQPKNTDA 216 A + R GL+Y +APYVSYA SF P GS+ PF PT +Q E G+K+QP + Sbjct: 468 ALSGRIGLSYLVTRDIAPYVSYATSFLPQTGSDFNGKPFDPTKARQAEVGIKYQPADGKT 527 Query: 217 LFTLAVYDLRQQNVLTPDPVFPTRSVQTGEIRSRGIEFEAHANLTENLKAIASINKMVQD 276 LFT A+++L + NV TPDP +V +GEI+SRG+E EA + + L AS Sbjct: 528 LFTAALFELTKTNVQTPDPAHTNFNVLSGEIKSRGLELEAKGEIVKGLNLSASYTYTDVK 587 Query: 277 IPRSNTAT-RGKRPVAQPGQMASAWLDYTIHGGSLDKLGFAGGVRYVGSSYGTADNLTTV 335 ++NTA+ GK+P+ P Q AS W DYT+ GG L G GG RY GS+Y A N Sbjct: 588 NTQNNTASLLGKQPILVPRQAASVWADYTVQGGPLAGAGIGGGARYTGSNYADAANTVKN 647 Query: 336 GGRTLFDAAIHYDYDQHWKFALNAANLFNKEYLAYCSNG-FLCYWGATRTVLATARYQW 393 GG T+FDA HY+ ++ W++ALN +N+ NKEY + + C+W A RT + +ARY+W Sbjct: 648 GGVTVFDAVFHYNLNR-WRYALNISNITNKEYTSCLAEPTTTCFWAAERTAILSARYRW 705 >gi|300311652|ref|YP_003775744.1| TonB-dependent siderophore receptor protein [Herbaspirillum seropedicae SmR1] Length = 832 Score = 219 bits (559), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 131/359 (36%), Positives = 181/359 (50%), Gaps = 9/359 (2%) Query: 40 HLTNFAVDNQAQIKFNTGALAHTTLVGIDYQRTLGGVNRGGAVTGSINIYNPNFAALSTA 99 + N +DNQA + G H L+G+D+ R V S++I NP + A Sbjct: 478 QVENTLLDNQALARVQWGQAEHQVLLGLDWSHMSAREQRYRGVAPSLDIDNPVYTQPIPA 537 Query: 100 QITTFEDY--SLTQVGAYLQDQVKLGNWSLQLGGRKDWTTSNDVQRTGAVTTVARQNDSA 157 T F D +L Q G Y QDQ++ L LG R D S D R A ++ ++A Sbjct: 538 ATTRFGDLDQTLVQTGLYAQDQIRYQRIVLTLGARYD--RSRDSTRNAANNSLQAAEENA 595 Query: 158 FTWRGGLTYQFDSGLAPYVSYAKSFQPTIGSNTGAAPFVPTTGKQYEAGVKFQPKNTDAL 217 F+ R GL+Y LAPYVSY SF P G + PF T +Q EAGVK+QP + A+ Sbjct: 596 FSGRVGLSYLVSPELAPYVSYGSSFLPQAGQDVDGKPFKAATARQIEAGVKYQPGHGRAV 655 Query: 218 FTLAVYDLRQQNVLTPDPVFPTRSVQTGEIRSRGIEFEAHANLTENLKAIASINKMVQDI 277 +T A+Y L + N L DP+ SV G++RSRG+E EA L L A+ Q + Sbjct: 656 YTAALYQLVKDNALGSDPLHANFSVSNGQVRSRGVELEAKGELLPGLNVTAAYT-YAQVV 714 Query: 278 PRSNTATR--GKRPVAQPGQMASAWLDYTIHGGSLDKLGFAGGVRYVGSSYGTADNLTTV 335 N A GK P+ P Q AS WLDYT+ G L +G G RY G +Y TA N Sbjct: 715 NTQNAAPELIGKTPILVPRQAASLWLDYTVQRGDLAGMGVGAGARYTGRNYATAANTVEN 774 Query: 336 GGRTLFDAAIHYDYDQHWKFALNAANLFNKEYLAYCSNGFL-CYWGATRTVLATARYQW 393 + + DA + D W++A N +NL N++Y + + L C+W RT + +ARY+W Sbjct: 775 AAQVILDAMVRIDRGP-WRYAFNVSNLANRQYTSCLAEPTLTCFWAPERTAVLSARYRW 832 >gi|300313794|ref|YP_003777886.1| ferrichrome-iron receptor protein [Herbaspirillum seropedicae SmR1] Length = 817 Score = 201 bits (511), Expect = 9e-53, Method: Compositional matrix adjust. Identities = 152/411 (36%), Positives = 210/411 (51%), Gaps = 31/411 (7%) Query: 3 QNVRYLHDDIDY--YQTSITGPLAADNRTVPI-WSNI------NREHLTNFAVDNQAQIK 53 Q+ R H DY QT L A N P+ + N+ +RE T F++DNQ Q Sbjct: 418 QSARVGHMSTDYRAVQTPRFATLNAANPNDPVNFQNLTRNLFGSRESATMFSIDNQLQAD 477 Query: 54 FNTGALAHTTLVGIDYQRT-LGGVNRGGAVTGSINIYNPNFAALSTAQITTFEDYS--LT 110 F+TGA+ HT L G+DYQR+ V+ G +NI P++ + D LT Sbjct: 478 FSTGAVRHTVLAGVDYQRSRFDAVSFSGGSAPLLNIAAPSYPNGPFVVPAPYSDNRAVLT 537 Query: 111 QVGAYLQDQVKLGN-WSLQLGGRKDWTTSN-DVQRTGAVTTVARQNDSAFTWRGGLTYQF 168 Q G YLQDQ+K N W + LGGR D +S D + G+ T+ D FT R + Y Sbjct: 538 QTGIYLQDQIKWNNGWEVTLGGRHDSASSTVDNRLDGSRTS---STDRKFTRRAAVLYHA 594 Query: 169 DSGLAPYVSYAKSFQPTIGSNTGA-APFVPTTGKQYEAGVKFQPKNTDALFTLAVYDLRQ 227 +GLAPY+SY +SF PT N F P TGKQ E G+++QP T+ L++ A++DL++ Sbjct: 595 ANGLAPYLSYTESFNPTATVNPLTNQSFGPETGKQTEVGLRYQPPGTNDLYSAALFDLKR 654 Query: 228 QNVLTPDPVF-PTRSVQTGEIRSRGIEFEAHANLTENLKAIASINKMVQDI--PRSNTAT 284 QN ++ D F PT Q GEI RG+E EA L+ A+ + + + S A Sbjct: 655 QNYISYDANFMPT---QKGEIAVRGLELEAVTQPMAALRLTAAYSYTPRAVVTASSRPAE 711 Query: 285 RGKRPVAQPGQMASAWLDYTIHGGSLDKLGFAGGVRYVGSSYGTADNL-TTVGGRTLFDA 343 GK+ A S W D+ + G +LG G RYV ++ G V G TL D Sbjct: 712 IGKQAAAVLLHQFSVWGDHRLGNGV--RLGL--GARYVSATSGNGGGTPVKVPGYTLLDG 767 Query: 344 AIHYDYDQHWKFALNAANLFNKEYLAYC-SNGFLCYWGATRTVLATARYQW 393 + YD+ W ALN NL +K YL C + +CY+G R+VLATA Y+W Sbjct: 768 LLGYDF-ARWSLALNLRNLTDKRYLTNCDTTASMCYYGDQRSVLATASYRW 817 >gi|300310859|ref|YP_003774951.1| TonB-dependent siderophore receptor protein [Herbaspirillum seropedicae SmR1] Length = 705 Score = 155 bits (392), Expect = 5e-39, Method: Compositional matrix adjust. Identities = 121/350 (34%), Positives = 167/350 (47%), Gaps = 23/350 (6%) Query: 49 QAQI--KFNTGALAHTTLVGIDY------QRTLGGVNRGGAVTGSINIYNPNFA-ALSTA 99 QA+I KF T + H LVG + QR L +N ++NIYNP + A T Sbjct: 354 QAEIGGKFATAGVGHEVLVGTEVDWLTLDQRMLR-INPSNVAPYAVNIYNPVYGQAQPTP 412 Query: 100 QITTFEDYSLTQVGAYLQDQVKLGN-WSLQLGGRKD--WTTSNDVQRTGAVTTVARQNDS 156 T G YLQDQ+ L W L G R D +T N++ R GAVT RQ+ Sbjct: 413 TANTDTSERQISTGLYLQDQLSLSERWRLLAGVRADAFRSTLNNL-RNGAVT---RQDKH 468 Query: 157 AFTWRGGLTYQFDSGLAPYVSYAKSFQPTIGSNTGAAPFVPTTGKQYEAGVKFQPKNTDA 216 A++ R GLTY + YVS +KSF+P GS+ F P T + EAG+KFQ ++ + Sbjct: 469 AYSPRLGLTYLLTPSWSVYVSASKSFRPNSGSDVSGNAFSPETSRAAEAGLKFQSEDKRS 528 Query: 217 LFTLAVYDLRQQNVLTPDPVFPTRSVQTGEIRSRGIEFEAHANLTENLKAIASINKMVQD 276 TLAV+D+ +QNVLT DP P S+ GE+RS+G+E +A+A L + + ++ Sbjct: 529 GATLAVFDITKQNVLTTDPANPNFSIAAGEVRSKGVELDANARLGHHWRVTGNLAYTDAY 588 Query: 277 IPRSNTATRGKRPVAQPGQMAS---AWLDYTIHGGSLDKLGFAGGVRYVGSSYGTADNLT 333 + R NT G R V P AS + D HG G GG+ YVG G Sbjct: 589 VSRDNTLPVGSRLVNIPRSSASLLAVFEDADAHGA---PYGVGGGLNYVGRRTGDQAGSF 645 Query: 334 TVGGRTLFDAAIHYDYDQHWKFALNAANLFNKEYLAYCSNGFLCYWGATR 383 + A ++ + K +L+ NLFNK Y + N G R Sbjct: 646 MLPSYVTARALAYWQFTPKMKLSLDVNNLFNKRYYSSSYNSLWIMPGDER 695 >gi|300310824|ref|YP_003774916.1| hydroxamate-type ferrisiderophore receptor signal peptide protein, tonB-dependent receptor [Herbaspirillum seropedicae SmR1] Length = 715 Score = 108 bits (271), Expect = 5e-25, Method: Compositional matrix adjust. Identities = 119/424 (28%), Positives = 182/424 (42%), Gaps = 69/424 (16%) Query: 8 LHDDIDYYQTSITGPLAADNRTVPIWSNINREHLTN------FAVDNQAQIKFNTGALAH 61 L++++ Y Q +++G L + N +++ R + F +D Q F T H Sbjct: 323 LNNNLRYQQFTMSGQLISINNLTG--TSLTRSAMDQTFEGQTFTMDTNVQRSFATAWGKH 380 Query: 62 TTLVGIDY------QRTLGGVNRGGAVTGSINIYNPNFAA--LSTAQITT----FEDYSL 109 +G DY +RTL +VT NIYNP++ A + Q+T+ D S+ Sbjct: 381 EVTLGTDYMNSRELERTLTC-----SVTPLSNIYNPSYGARVVCPTQLTSTNAKLNDTSV 435 Query: 110 TQVGAYLQDQVKLGN-WSLQLGGRKDWTTSNDVQRTGAVTTVARQNDSAFTWRGG-LTYQ 167 G Y +D++ LG W L G R+D + T A+T N S+ T + + Sbjct: 436 RDTGFYARDKISLGERWQLLAGVRQDRAAT---YTTSALTGSHSDNASSATTASAAIMVE 492 Query: 168 FDSGLAPYVSYAKSFQPTIGSNTGAAPFVPTTGKQYEAGVKFQPKNTDALFTLAVYDLRQ 227 G+ PY+SYA SF P G+ + F P TGKQ+E GVK A++DLR+ Sbjct: 493 LWHGVRPYLSYATSFYPNSGTAFDGSIFKPETGKQWETGVKVDLDEGRTSINFALFDLRR 552 Query: 228 QNVLTPDPVFPTRSVQTGEIRSRGIEFEAHANLTENLKAIA------------------S 269 +NVL DP + + G RS+G E ++L L A + Sbjct: 553 RNVLVTDPDHTSYKMAIGGQRSQGGEISVTSDLRNGLSLNAAYSYTAAIVIDDGGQRSTT 612 Query: 270 INKMVQDIPRSNTATRGKRPVAQPGQMASAWLDYTIHGGSLDKLGFAGGVRYVGSSYGTA 329 + + + ++PR N T + + G L GGVR G S Sbjct: 613 VGQWLDNVPRHNFTTSARYRFS----------------GELSGWELNGGVR--GQSM-MR 653 Query: 330 DNLTTVGGRTLFDAAIHYDYDQHWKFALNAANLFNKEYLAYCSNGFLCYWGATRTVLATA 389 N T+ G L D + Y+ ++ W+ ALN N+FN EY A G G RTV+ T Sbjct: 654 TNGYTLPGYVLADIGVAYNAER-WRTALNVKNVFNTEYYA-GGLGKAVALGDDRTVMLTL 711 Query: 390 RYQW 393 Y++ Sbjct: 712 GYRY 715 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,252,145 Number of extensions: 51888 Number of successful extensions: 185 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 151 Number of HSP's successfully gapped: 18 Length of query: 393 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 306 Effective length of database: 1,207,925 Effective search space: 369625050 Effective search space used: 369625050 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)