BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0451.p (383 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311746|ref|YP_003775838.1| iron-regulated membrane protein... 59 7e-10 >gi|300311746|ref|YP_003775838.1| iron-regulated membrane protein [Herbaspirillum seropedicae SmR1] Length = 435 Score = 58.9 bits (141), Expect = 7e-10, Method: Compositional matrix adjust. Identities = 59/213 (27%), Positives = 91/213 (42%), Gaps = 31/213 (14%) Query: 12 VHKWTSLVSTVFLLLLCLTGLPLIFGDEIDAVFDPEVAISAVAAG-TPDASLDRII---A 67 +H+WT L FL++ LTG L+F DE+DA P++ ++ A G + A +D + Sbjct: 25 LHRWTGLGMAFFLIVTSLTGSLLVFDDELDAWLAPQLQLAQPAPGQSAQAMMDPYLLREK 84 Query: 68 AAHARYPGQHLRFMVWDKEEPRRLSLNMAASADATAAQGHVLIYD-------------TR 114 A A P H+ +V R +S + D + + L YD R Sbjct: 85 VAQAMGPQVHISQLVLHARPGRTVSFQADPATDPATGKPYRLGYDEILVNPYTGVVQGVR 144 Query: 115 TATLLSETPALHGFMHVMLQLHQNLFIGLA-GDLLLGVAGLLFLAALVSGVVLYGPYMRK 173 +S PA M + +LH +L + A G LLLG GLL+ G++L P R Sbjct: 145 DRDKISLKPA--DLMPFLFKLHHSLALPRAPGALLLGTVGLLWTLDCFVGMILTWPRARP 202 Query: 174 LDFGAVRFARGRRLRW--------LDLHNLIGV 198 V++ ++W LDLH G+ Sbjct: 203 F---LVKWKPAWLIKWRAGFYRVNLDLHRAFGL 232 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.139 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,049,624 Number of extensions: 36027 Number of successful extensions: 219 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 219 Number of HSP's successfully gapped: 1 Length of query: 383 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 296 Effective length of database: 1,207,925 Effective search space: 357545800 Effective search space used: 357545800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 131 (55.1 bits)