BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0448.p (880 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313026|ref|YP_003777118.1| signal transduction protein con... 410 e-115 gi|300314042|ref|YP_003778134.1| signal transduction protein con... 171 3e-43 gi|300312026|ref|YP_003776118.1| signal transduction protein con... 169 1e-42 gi|300311038|ref|YP_003775130.1| hypothetical protein Hsero_1709... 162 8e-41 gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodies... 160 4e-40 >gi|300313026|ref|YP_003777118.1| signal transduction protein containing sensor and EAL domains protein [Herbaspirillum seropedicae SmR1] Length = 544 Score = 410 bits (1054), Expect = e-115, Method: Compositional matrix adjust. Identities = 202/440 (45%), Positives = 298/440 (67%), Gaps = 9/440 (2%) Query: 422 DHIARMRRLTANTRTVDEIGYFENGLLKCTSW--GLVNERVEQTPADFVTEDGLEVTLRM 479 +HIA+MR ++ N+R+++E+GY+E+G LKCTSW + R Q F T DG+EV L Sbjct: 78 EHIAQMRLISVNSRSIEEMGYYEHGQLKCTSWDSSVGARRRRQAEPVFTTADGVEVALDQ 137 Query: 480 QPLITQGNAMMALQYRSHNVLTVPSRFSDVILDPGMRLAVATGSGHILGDLNAPDPDVVR 539 + + G M+++QY +H+ L RF DV+L+ G+ + +A + P V Sbjct: 138 RSFVADGPTMLSIQYGAHSALVHRLRFVDVVLEKGVHVVLARNGFGPVAQYGGP---VAA 194 Query: 540 RVVANPANGID----DERLIAVARGDNMVAVAMEPRSFLFERMREEELRLVPIGVLLSVL 595 V + +G+ D I+ D A A+ + E M ++ +P+G+LL + Sbjct: 195 GVAEHALDGLGRQDVDGFFISTVSRDGWTAAAIVDGDHVLEEMTGQQRWFLPVGILLGIG 254 Query: 596 MVGLVMWSLRRRLSPLGELEIAVKKREFIVHYQPIIELAGGNCVGAEALVRWLRPDGSLV 655 +V LV+ + RLSPL +L++AVKK+EFIVHYQPIIE+A G C+GAEAL+RW RPDGS+V Sbjct: 255 LVMLVLRVSQARLSPLADLQLAVKKKEFIVHYQPIIEMASGRCIGAEALLRWRRPDGSIV 314 Query: 656 QPDLFIPLAEDSGLIMAITDQVIDEVMEDLGELLINDRSVHIAINIGADDIRSGRVIDVL 715 +PD FI LAE+SGLI+ +TD V+D V+ ++ + L DR++HIAIN+ A D+++GR+++V+ Sbjct: 315 RPDFFIALAEESGLILPLTDLVVDTVVREMRDTLRQDRALHIAINVAAQDMKTGRILEVV 374 Query: 716 QHALARSGVEAQQIWLEATERGFINVLPARLTLGNARALGHAVVIDDFGTGYSSLSHLQS 775 ALA S + +Q+W+EATERG +++ A LTL AR +GH++ +DDFGTGYS+L +LQ Sbjct: 375 SQALAGSDIRPEQLWMEATERGCMDIASASLTLARAREMGHSMALDDFGTGYSNLQYLQG 434 Query: 776 LPLDALKIDKSFIDTIGTDSAISSVTPHIIGMARTLFLKIIAEGVETEAQADYLRRQKVD 835 LP+DALKIDKSF+ TIGT +A S+V HII MA++L L I+AEGVET QA YL + VD Sbjct: 435 LPMDALKIDKSFVSTIGTGAATSAVIDHIIAMAKSLKLYIVAEGVETLEQAAYLAERGVD 494 Query: 836 YAQGWLYAMPMPAAAFIAYH 855 +AQGWL++ P+P F+A++ Sbjct: 495 FAQGWLFSRPLPVEEFVAFY 514 Score = 160 bits (404), Expect = 6e-40, Method: Compositional matrix adjust. Identities = 102/277 (36%), Positives = 155/277 (55%), Gaps = 9/277 (3%) Query: 25 MMYLAWTRAVNEEQARLELFARRAIQRANISLREIRSALYAVEPLNLLPCSSDHIARMRR 84 MM+L+ E RL +A I RA S E + +L ++ PL LLPCS +HIA+MR Sbjct: 26 MMWLSRQAVAQAEHERLARYAESVIARAAHSFDETQRSLKSLSPLLLLPCSDEHIAQMRL 85 Query: 85 LTANTRTVDEIGYFENGLLKCTSW--GLVNERVEQTPADFVTEDGLEVTLRMQPLITQGN 142 ++ N+R+++E+GY+E+G LKCTSW + R Q F T DG+EV L + + G Sbjct: 86 ISVNSRSIEEMGYYEHGQLKCTSWDSSVGARRRRQAEPVFTTADGVEVALDQRSFVADGP 145 Query: 143 AMMALQYRSHNVLTVPSRFSDVILDPGMRLAVATGSGHILGDLNAPDPDVVRRVVANPAN 202 M+++QY +H+ L RF DV+L+ G+ + +A + P V V + + Sbjct: 146 TMLSIQYGAHSALVHRLRFVDVVLEKGVHVVLARNGFGPVAQYGGP---VAAGVAEHALD 202 Query: 203 GID----DERLIAVARGDNMVAVAMEPRSFLFERMREEELRLVPIGVLLSVLMVGLVMWS 258 G+ D I+ D A A+ + E M ++ +P+G+LL + +V LV+ Sbjct: 203 GLGRQDVDGFFISTVSRDGWTAAAIVDGDHVLEEMTGQQRWFLPVGILLGIGLVMLVLRV 262 Query: 259 LRRRLSPLGELEIAVKKREFIVHYQPIIELAGGNAPG 295 + RLSPL +L++AVKK+EFIVHYQPIIE+A G G Sbjct: 263 SQARLSPLADLQLAVKKKEFIVHYQPIIEMASGRCIG 299 >gi|300314042|ref|YP_003778134.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 948 Score = 171 bits (432), Expect = 3e-43, Method: Compositional matrix adjust. Identities = 97/255 (38%), Positives = 149/255 (58%), Gaps = 2/255 (0%) Query: 607 RLSPLGELEIAVKKREFIVHYQPIIELAGGNCVGAEALVRWLRPDGSLVQPDLFIPLAED 666 RL G+L A+ EF++HYQP ++LA G +G+EAL+RW P +V P FI LAE+ Sbjct: 691 RLRIEGDLRNALDHGEFLLHYQPKVDLASGRVIGSEALIRWQHPQLGMVSPARFIGLAEE 750 Query: 667 SGLIMAITDQVIDEVMEDLGELLINDRS-VHIAINIGADDIRSGRVIDVLQHALARSGVE 725 +GLI+ I V+ + + + + +++N+ ++ + L +G+ Sbjct: 751 TGLILPIGAWVLRAACTQCRQWQLGGYADISVSVNLSVGQFLQKDLVSSIADVLEDTGLP 810 Query: 726 AQQIWLEATERGFI-NVLPARLTLGNARALGHAVVIDDFGTGYSSLSHLQSLPLDALKID 784 + + LE TE + +V A L + LG + IDDFGTGYSSL++L+ P+D LKID Sbjct: 811 PESLELELTESLMMTDVEHAIGILAGLKKLGVRLSIDDFGTGYSSLAYLKRFPIDVLKID 870 Query: 785 KSFIDTIGTDSAISSVTPHIIGMARTLFLKIIAEGVETEAQADYLRRQKVDYAQGWLYAM 844 +SF+ I D+ +++T II +AR L L++IAEGVET+ Q DYLR D QG+L++ Sbjct: 871 RSFVRDITIDADDAAITASIISLARHLKLRVIAEGVETDEQLDYLRHYGCDEIQGYLFSP 930 Query: 845 PMPAAAFIAYHRQNR 859 P+PA F RQ+R Sbjct: 931 PVPADKFEQILRQDR 945 >gi|300312026|ref|YP_003776118.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1106 Score = 169 bits (427), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 100/248 (40%), Positives = 141/248 (56%), Gaps = 2/248 (0%) Query: 607 RLSPLGELEIAVKKREFIVHYQPIIELAGGNCVGAEALVRWLRPDGSLVQPDLFIPLAED 666 R++ EL A+++ EF+VHYQP IELA G VGAEALVRW P L+ P FI LAE+ Sbjct: 842 RIALEAELRRAIERAEFVVHYQPKIELAEGRIVGAEALVRWQHPLRGLLPPGEFITLAEE 901 Query: 667 SGLIMAITDQVIDEVMEDLGELLINDRSV-HIAINIGADDIRSGRVIDVLQHALARSGVE 725 + LI I V+D D+G + + I+IN+ R++D ++ + R G Sbjct: 902 TRLIDQIGLLVLDVACRDIGLMRQQQMTCGRISINLTGSQFGDERLLDDIKEVVDRHGTP 961 Query: 726 AQQIWLEATERGFI-NVLPARLTLGNARALGHAVVIDDFGTGYSSLSHLQSLPLDALKID 784 + E TE + N A + RA G + IDDFGTGYSSL++L+ P++ LKID Sbjct: 962 PSCLEFEITESMVMHNRERAIAIMDGIRAEGFTISIDDFGTGYSSLAYLKRFPVNTLKID 1021 Query: 785 KSFIDTIGTDSAISSVTPHIIGMARTLFLKIIAEGVETEAQADYLRRQKVDYAQGWLYAM 844 KSFI+ I + S++ II M+ L LK++ EGVETE Q LR D QG+ ++ Sbjct: 1022 KSFINDIPDNPNSSAIVQAIIAMSHALNLKVVTEGVETEKQLQALREFGSDEYQGYYFSK 1081 Query: 845 PMPAAAFI 852 P+P A F+ Sbjct: 1082 PVPYAQFV 1089 >gi|300311038|ref|YP_003775130.1| hypothetical protein Hsero_1709 [Herbaspirillum seropedicae SmR1] Length = 598 Score = 162 bits (411), Expect = 8e-41, Method: Compositional matrix adjust. Identities = 90/234 (38%), Positives = 130/234 (55%), Gaps = 1/234 (0%) Query: 622 EFIVHYQPIIELAGGNCVGAEALVRWLRPDGSLVQPDLFIPLAEDSGLIMAITDQVIDEV 681 E ++H+QP + L G CVG EAL+RW P + PD+FIPLAE +G I +T V+ +V Sbjct: 360 ELVLHFQPKVRLRDGACVGVEALIRWHHPIHGNIPPDVFIPLAEKTGQIRQVTAWVLRQV 419 Query: 682 MEDLGELLINDRSVHIAINIGADDIRSGRVIDVLQHALARSGVEAQQIWLEATERGFIN- 740 + + L + R++ +A N A D S + ++L L V I +E TE N Sbjct: 420 VAQVARWLKSGRALPVAFNASARDFDSDELRELLAQQLKLHKVPPALIEIEFTEHAMANH 479 Query: 741 VLPARLTLGNARALGHAVVIDDFGTGYSSLSHLQSLPLDALKIDKSFIDTIGTDSAISSV 800 R + R LG V IDDFG G+S+LS+L+++P D +KID+SFI + D A + Sbjct: 480 PQQVRQRVQALRELGVKVAIDDFGAGFSNLSYLKNIPADTMKIDRSFIHRLLDDEADQLI 539 Query: 801 TPHIIGMARTLFLKIIAEGVETEAQADYLRRQKVDYAQGWLYAMPMPAAAFIAY 854 P I+ +A L ++AEG+E+E A LR DY QG+ A PMP A A+ Sbjct: 540 VPSILHLAHNLGFSVVAEGLESERTATLLRHLGCDYGQGYAIARPMPLAELEAW 593 >gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor domains protein [Herbaspirillum seropedicae SmR1] Length = 701 Score = 160 bits (405), Expect = 4e-40, Method: Compositional matrix adjust. Identities = 97/262 (37%), Positives = 148/262 (56%), Gaps = 3/262 (1%) Query: 607 RLSPLGELEIAVKKREFIVHYQPIIELAGGNCVGAEALVRWLRPDGSLVQPDLFIPLAED 666 RL L L A+ + EF ++YQP + L G +GAEAL+RW P +V PD FIP AE+ Sbjct: 439 RLEMLINLRRALDQDEFKLYYQPKLSLQDGRLIGAEALIRWESPQQGMVPPDRFIPFAEE 498 Query: 667 SGLIMAITDQVIDEVM-EDLGELLINDRSVHIAINIGADDIRSGRVIDVLQHALARSGVE 725 +GLI+ I + V+ ++ + +A+N+ + + D + LA+SG+ Sbjct: 499 AGLIVPIGEWVLRTACRQNQAWQQAGLPPIPVAVNLSPRQLNQS-LPDFVAGVLAQSGLA 557 Query: 726 AQQIWLEATERGFI-NVLPARLTLGNARALGHAVVIDDFGTGYSSLSHLQSLPLDALKID 784 A + LE TE + + + TL + LG + +DDFGTGYSSLS+L+ P+DALKID Sbjct: 558 ASWLELEITENVVMKDAEKSVATLHALKRLGLQISVDDFGTGYSSLSYLRRFPVDALKID 617 Query: 785 KSFIDTIGTDSAISSVTPHIIGMARTLFLKIIAEGVETEAQADYLRRQKVDYAQGWLYAM 844 KSF+ I D+ +++ II +A L L++IAEGVE E Q +L+ D QG+ + Sbjct: 618 KSFVRDIVRDADSAAIVKAIISLAHILNLRVIAEGVEDEEQHAFLKENACDEVQGYYFGK 677 Query: 845 PMPAAAFIAYHRQNRSAQEVMA 866 PM A F A+ + A +V++ Sbjct: 678 PMEVAVFTAWMSEQAQAGQVLS 699 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.137 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,654,555 Number of extensions: 114568 Number of successful extensions: 407 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 385 Number of HSP's successfully gapped: 19 Length of query: 880 Length of database: 1,619,870 Length adjustment: 94 Effective length of query: 786 Effective length of database: 1,174,780 Effective search space: 923377080 Effective search space used: 923377080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 135 (56.6 bits)