BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0447.p (170 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310857|ref|YP_003774949.1| drug/metabolite transporter (DM... 94 8e-21 >gi|300310857|ref|YP_003774949.1| drug/metabolite transporter (DMT) superfamily permease [Herbaspirillum seropedicae SmR1] Length = 298 Score = 93.6 bits (231), Expect = 8e-21, Method: Compositional matrix adjust. Identities = 52/112 (46%), Positives = 75/112 (66%), Gaps = 2/112 (1%) Query: 33 LPLLAVCIWSGNTIVTKAASTAIEPAAIAFYRWVLAVLVMTPFLGRAAWRQRAAIAADWN 92 PL AV IW+GNT+++K +++ I P I+FYRWVLA L+M+PFL R W RA I + Sbjct: 8 FPLGAVLIWAGNTVISKMSASVIAPEDISFYRWVLAALLMSPFLARQTWAARAQIRP--H 65 Query: 93 WARLLLLGALGMAMYQGLAYEAAHSTSAVNMGVTVALMPLISACLSSMLSGE 144 ++++L LGM ++Q LAY AA + SA +MG+ +LMPL++ LS L E Sbjct: 66 LGKIVVLALLGMVLFQSLAYFAAATASATSMGIIASLMPLLTLVLSIKLLSE 117 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.130 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 277,674 Number of extensions: 6093 Number of successful extensions: 71 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 73 Number of HSP's successfully gapped: 1 Length of query: 170 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 91 Effective length of database: 1,245,805 Effective search space: 113368255 Effective search space used: 113368255 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 127 (53.5 bits)