BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0445.p (231 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309871|ref|YP_003773963.1| transcription regulator protein... 158 3e-40 gi|300313281|ref|YP_003777373.1| LysR family transcription regul... 98 7e-22 gi|300314079|ref|YP_003778171.1| LysR family transcription regul... 90 2e-19 gi|300310448|ref|YP_003774540.1| transcription regulator protein... 82 4e-17 gi|300312554|ref|YP_003776646.1| transcription regulator protein... 77 9e-16 >gi|300309871|ref|YP_003773963.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 332 Score = 158 bits (400), Expect = 3e-40, Method: Compositional matrix adjust. Identities = 90/220 (40%), Positives = 125/220 (56%), Gaps = 10/220 (4%) Query: 16 QVDLSTVERTMVLRTNDGFAGAWAARLAQRVAQEAPGISLRFLPRADKHVAGLRDGLIDL 75 ++DLS +ER +R NDGF A A+L + V EAP + LRF+P+ ++ LR+G DL Sbjct: 106 ELDLSRLERDFSIRANDGFIEALGAQLIEAVTAEAPLVRLRFVPKVERGPVPLREGSADL 165 Query: 76 EVGALHEGDDTSEELHTQLLFRTPFVGVVRADHPLAR-KKISLANFTAWPHISASRHGHN 134 E+G E E+ Q LFR FVG VR HPLA +++S + A+ H+SAS G Sbjct: 166 EIGVASE---MGPEVRLQTLFRDRFVGAVRTGHPLAALRRVSAERYCAFDHVSASPRGVA 222 Query: 135 SGPIDDALEARGLQRKVRLVAPGFQSAMVMAASSDMVAAAPELFARWAMQHLR------L 188 +GP+ +AL ARGL R+V V P F A+ + +SD+V P F A + + Sbjct: 223 AGPLHEALAARGLVRRVVAVVPSFPGALAVVRTSDLVTMVPGSFLTRAGAERKDGSAAGI 282 Query: 189 HIFRLPLATPEVEVSQSWHPRRHADPVHKWLRGLVRELCS 228 H+F LPL + VS WHPR DP H+WLR VR +C+ Sbjct: 283 HVFELPLPLEPIIVSLMWHPRVELDPAHRWLRDKVRAVCA 322 >gi|300313281|ref|YP_003777373.1| LysR family transcription regulator [Herbaspirillum seropedicae SmR1] Length = 323 Score = 97.8 bits (242), Expect = 7e-22, Method: Compositional matrix adjust. Identities = 68/223 (30%), Positives = 106/223 (47%), Gaps = 3/223 (1%) Query: 10 KVFMTDQVDLSTVERTMVLRTNDGFAGAWAARLAQRVAQEAPGISLRFLPRADKH-VAGL 68 +V +DQ +T + VL +D RL +R+ EAP S+ + A VA L Sbjct: 81 EVLSSDQFTPATTTKRFVLALSDIGELMLLPRLLERIRDEAPHASVVTVTMAPAELVAAL 140 Query: 69 RDGLIDLEVGALHEGDDTSEELHTQLLFRTPFVGVVRADHPLARKKISLANFTAWPHISA 128 G +DL +G + D + Q LF FV ++RA HPL +KISL ++ A HI Sbjct: 141 ESGEVDLAIG--YFPDLRNRNFFQQRLFSQDFVCLLRAGHPLPAQKISLEHYQAAGHIVV 198 Query: 129 SRHGHNSGPIDDALEARGLQRKVRLVAPGFQSAMVMAASSDMVAAAPELFARWAMQHLRL 188 G + + L R ++R+V L F + ++ A+SD+VA P F + ++ + Sbjct: 199 KAEGRDQPMCERFLLRRKIRRRVVLSTAHFMAIPLLVATSDLVATVPRAFGEFVVRFAGI 258 Query: 189 HIFRLPLATPEVEVSQSWHPRRHADPVHKWLRGLVRELCSDGA 231 + P +++ Q WH + H D + WLRGL L GA Sbjct: 259 RLVEPGFEMPMLDLKQHWHRKYHKDAGNAWLRGLAAALFPAGA 301 >gi|300314079|ref|YP_003778171.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 319 Score = 89.7 bits (221), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 69/226 (30%), Positives = 102/226 (45%), Gaps = 13/226 (5%) Query: 7 EMRKVFMTDQVDLSTVERTMVLRTNDGFAGAWAARLAQRVAQEAPGISLRFLPRADKHV- 65 E+ V + D+S + T + D A W L + + ++AP I +R +P + Sbjct: 79 ELEAVIAPESFDISRAQNTFRMAMVDATAALWLPSLVRTIMRDAPRIKIRMMPLTTRDPR 138 Query: 66 AGLRDGLIDLEVG---------ALHEGDDTSEELHTQLLFRTPFVGVVRADHPLARKKIS 116 A L G IDL VG A +G S+ H +L + +V V+ HPLA + ++ Sbjct: 139 AMLLRGDIDLAVGFFPGVTAQLADGQGMAKSQISHARL-YTGQYVCVMHKSHPLAGQPLT 197 Query: 117 LANFTAWPHISASRHGHNSGPIDDALEARGLQRKVRLVAPGFQSAMVMAASSDMVAAAPE 176 L ++ H+ S G IDD L +RK+ L F +A + ASS+++ P Sbjct: 198 LDSYCNADHLLVSLSGKPHAVIDDVLAGMNRERKILLTVNQFFTAGRIVASSELITVLPR 257 Query: 177 -LFARWAMQHLRLHIFRLPLATPEVEVSQSWHPRRHADPVHKWLRG 221 L A M L + LP TPE V WH R +P HKWLR Sbjct: 258 HLVAATGMAE-ALVVKELPFQTPEEHVDMLWHERDARNPAHKWLRA 302 >gi|300310448|ref|YP_003774540.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 306 Score = 82.0 bits (201), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 63/227 (27%), Positives = 99/227 (43%), Gaps = 7/227 (3%) Query: 3 ALAQEMRKVFMTDQVDLSTVERTMVLRTNDGFAGAWAARLAQRVAQEAPGISLRFL-PRA 61 AL Q R + + + T R + D + L + A+ APG+S+ + A Sbjct: 79 ALQQVARALNLQEGFAPQTSARRFTIAMTDVGEVYFMPALIEACARCAPGVSISTVRAGA 138 Query: 62 DKHVAGLRDGLIDLEVGALHEGDDTSEELHTQLLFRTPFVGVVRADHPL---ARKKISLA 118 + + G +DL +GA D+ SE L+ + LFR +V + R H L R ++SL Sbjct: 139 IDLKSEMESGRVDLAIGAF---DNLSEALYQRRLFRQEYVSMFRIGHALDKPGRGEVSLK 195 Query: 119 NFTAWPHISASRHGHNSGPIDDALEARGLQRKVRLVAPGFQSAMVMAASSDMVAAAPELF 178 F A H+ S I+ LE G+ VR P F + + +S+ +V P Sbjct: 196 EFLAAEHLMVSSQESPYDRINQNLEKAGILPSVRFRVPHFTAVPYIVSSTALVVTVPRKL 255 Query: 179 ARWAMQHLRLHIFRLPLATPEVEVSQSWHPRRHADPVHKWLRGLVRE 225 A A L + PL P ++ + WH R + D + WLRG + E Sbjct: 256 AESAAAPFGLRYIKPPLRLPSLQTNMFWHRRYNQDEGNVWLRGFISE 302 >gi|300312554|ref|YP_003776646.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 314 Score = 77.4 bits (189), Expect = 9e-16, Method: Compositional matrix adjust. Identities = 62/209 (29%), Positives = 98/209 (46%), Gaps = 12/209 (5%) Query: 23 ERTMVLRTNDGFAGAWAARLAQRVAQEAPG--ISLRFLPRADKHVAGLRDGLIDLEVGAL 80 ER + D + + R+ +E+P I + L +V L DG +DL + Sbjct: 98 ERIFHIAAPDYMNTPFFTEVIARLRRESPRSRIVIHALGPDTDYVRRLSDGELDLVIA-- 155 Query: 81 HEGDDTSEELHTQLLFRTPFVGVVRADHPLARKK----ISLANFTAWPHISASR--HGHN 134 D LH LF P V ++RAD AR+ +S+ ++ + PH + S+ G++ Sbjct: 156 -NWDAPPAHLHLSKLFDDPIVCLMRADCAYARRTAREAMSVEDYLSLPHAAPSQILPGYH 214 Query: 135 SGPIDDALEARGLQRKVRLVAPGFQSAMVMAASSDMVAAAPELFARWAMQHLRLHIFRLP 194 G ID LE + L+R V + + F+ M A +D+V A + FA + + L L + +P Sbjct: 215 -GSIDSFLEKQNLRRNVVVESAHFRMLPYMVAQTDLVLTAGQQFASFYEKLLSLKSYAVP 273 Query: 195 LATPEVEVSQSWHPRRHADPVHKWLRGLV 223 + P + Q WH R H HKWLR V Sbjct: 274 IKFPPLRFYQLWHERSHQATEHKWLRAQV 302 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.134 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 735,254 Number of extensions: 28483 Number of successful extensions: 101 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 93 Number of HSP's successfully gapped: 7 Length of query: 231 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 149 Effective length of database: 1,231,600 Effective search space: 183508400 Effective search space used: 183508400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 129 (54.3 bits)