BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0034.0428.p
         (592 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300311830|ref|YP_003775922.1| isocitrate dehydrogenase [Herba...   968   0.0  

>gi|300311830|ref|YP_003775922.1| isocitrate dehydrogenase
           [Herbaspirillum seropedicae SmR1]
          Length = 742

 Score =  968 bits (2502), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 464/592 (78%), Positives = 517/592 (87%), Gaps = 6/592 (1%)

Query: 1   VTTDNSTIIYTLTDEAPLLATYSLLPIIRTFTAPAGTSXAGVNVVESDISVATRILAEFP 60
           +TT N TIIYTLTDEAP LAT+SLLPI++ FTA      AG++VVESDISVA RILAEFP
Sbjct: 1   MTTGNPTIIYTLTDEAPYLATHSLLPIVKKFTA-----AAGIDVVESDISVAARILAEFP 55

Query: 61  DRLTPEQRVPDNLAALGKLTQDPSANIIKLPNISASVPQLQAAIRELQSRGYAIPDLPED 120
           + LT EQ+VPDNLAALG LTQ P ANIIKLPNISAS+ QLQAAIRELQSRGY +PD PE+
Sbjct: 56  EYLTDEQKVPDNLAALGALTQSPDANIIKLPNISASILQLQAAIRELQSRGYKLPDYPEN 115

Query: 121 PKTDEEKEILKSYSKVLGSAVNPVLREGNSDRRAPASIKRFARKHPHSMGEWSMASRSHV 180
           P T+EEK + K Y+KV GSAVNPVLREGNSDRRAP ++K +ARKHPHSM +WSMASR+HV
Sbjct: 116 PTTEEEKALQKRYAKVTGSAVNPVLREGNSDRRAPNAVKNYARKHPHSMAKWSMASRTHV 175

Query: 181 AHMTHGDFYHGEKSMTIGAARDVKMELIGKSGKTTVLKAKVALKAGEIIDSMFMSKKALC 240
           AHM  GDFY GEKS+T+  A DVKM+L+ KSG+T VLK K++L+AGEIIDSMFMSKKALC
Sbjct: 176 AHMHGGDFYAGEKSLTLDKAVDVKMDLVTKSGETIVLKPKISLQAGEIIDSMFMSKKALC 235

Query: 241 EFYEEQFEDARKTGVMLSLHVKATMMKVSHPIVFGHAVKIFYKEAFAKHGKLFDELGVNV 300
            +YEEQ +DA +T ++LS+HVKATMMKVSHPIVFGHAV+ +YK+ F KH KLF E+GVN 
Sbjct: 236 AYYEEQMQDAFETDLLLSVHVKATMMKVSHPIVFGHAVRTYYKDTFTKHAKLFAEIGVNP 295

Query: 301 NNGLVNLYEKIETLPSSKKEEIIRDLHACHEHRPSLAMVDSAKGISNLHAPNDVIVDASM 360
           NNG+  +YEKI TLP +KK E++ DL A    RP LAMVDSAKGI+NLHAPNDVIVDASM
Sbjct: 296 NNGMSGVYEKINTLPDAKKAEVLADLKADEAKRPRLAMVDSAKGITNLHAPNDVIVDASM 355

Query: 361 PAMIRIGGKMWDANGKPQDTKAVIPESTFARIYQEVINFCKTNGNFDPTTMGTVPNVGLM 420
           PAMIR GGKMW+A GK +DTKA++PESTFARIYQE+INFCKTNG FDPTTMGTVPNVGLM
Sbjct: 356 PAMIRAGGKMWNAAGKTEDTKALLPESTFARIYQEMINFCKTNGAFDPTTMGTVPNVGLM 415

Query: 421 AQKAEEYGSHDKTFEIAEDGVANIVDLATGEVLLTQNVEQGDIWRMCQVKDEPIRDWVKL 480
           AQKAEEYGSHDKTFEIA+ GVA IV L  G+VLL QNVE+GDIWRMCQVKD  +RDWVKL
Sbjct: 416 AQKAEEYGSHDKTFEIAQAGVARIVTL-DGQVLLEQNVEEGDIWRMCQVKDAAVRDWVKL 474

Query: 481 AVTRARNSGMPAVFWLDPYRPHEAELIKKVETYLKDHDTKGLDIQIMSQVRAMRYTLERV 540
           AVTRAR SGMPAVFWLDPYRPHEAELIKKV TYLKDHD  G DIQIMSQVRAMRYTLERV
Sbjct: 475 AVTRARLSGMPAVFWLDPYRPHEAELIKKVNTYLKDHDLTGADIQIMSQVRAMRYTLERV 534

Query: 541 IRGLDTISVTGNILRDYLTDLFPIMELGTSAKMLSIVPLMAGGGMYETGAGG 592
           IRGLDTISVTGNILRDYLTDLFPIMELGTSAKMLSIVPLMAGGGM+ETGAGG
Sbjct: 535 IRGLDTISVTGNILRDYLTDLFPIMELGTSAKMLSIVPLMAGGGMFETGAGG 586


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.317    0.133    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,701,101
Number of extensions: 68259
Number of successful extensions: 213
Number of sequences better than 1.0e-08: 1
Number of HSP's gapped: 211
Number of HSP's successfully gapped: 1
Length of query: 592
Length of database: 1,619,870
Length adjustment: 91
Effective length of query: 501
Effective length of database: 1,188,985
Effective search space: 595681485
Effective search space used: 595681485
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 133 (55.8 bits)