BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0421.p (150 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309383|ref|YP_003773475.1| permease [Herbaspirillum serope... 188 1e-49 gi|300309898|ref|YP_003773990.1| major facilitator superfamily p... 68 4e-13 >gi|300309383|ref|YP_003773475.1| permease [Herbaspirillum seropedicae SmR1] Length = 412 Score = 188 bits (478), Expect = 1e-49, Method: Compositional matrix adjust. Identities = 97/125 (77%), Positives = 110/125 (88%) Query: 13 SGIAVLLVASLAIGLSATLAQDIVPAAATLASEQNRGKVVGTVMTGLLLGILLSRVLSGF 72 SG+ LL ASLA+GL+ATLAQDIVPAAA+LA EQ+RG+VVGTVMTGLLLGILLSRV+SGF Sbjct: 112 SGVGALLAASLAVGLTATLAQDIVPAAASLAPEQSRGRVVGTVMTGLLLGILLSRVVSGF 171 Query: 73 VAEHFGWRSMFVAASVSIALIGIAAWRGLPRFKATTHLPYGALLGSLGKLVQKHPALRRA 132 VAEHFGWR+MFVAA+VSIALIG+AAWRGLPRF TTHL YGAL+ SL L +KH ALRRA Sbjct: 172 VAEHFGWRAMFVAAAVSIALIGLAAWRGLPRFHPTTHLSYGALMASLATLFKKHGALRRA 231 Query: 133 SRCAG 137 + G Sbjct: 232 ALAQG 236 >gi|300309898|ref|YP_003773990.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 392 Score = 67.8 bits (164), Expect = 4e-13, Method: Compositional matrix adjust. Identities = 45/131 (34%), Positives = 72/131 (54%), Gaps = 2/131 (1%) Query: 2 LTGALLLAGASSGIAVLLVASLAIGLSAT-LAQDIVPAAATLASEQNRGKVVGTVMTGLL 60 L AL+ G + VLL LA+GL T + Q ++ AA+ A E RG+VVG +G+ Sbjct: 86 LVCALVTVGLARSSYVLLAGMLAVGLLGTAMTQGLIAYAASAAGEDERGRVVGAAQSGVF 145 Query: 61 LGILLSRVLSGFVAEHFGWRSMFVAASVSIALIGIAAWRGLPRFKATTH-LPYGALLGSL 119 +G+LLSRV SG V++ GWR +++ A++ + + W+ LP L Y L+ S+ Sbjct: 146 IGLLLSRVFSGAVSDVTGWRGVYLCAAIMMLCLAWPLWKRLPAIPVQADGLRYLTLIKSM 205 Query: 120 GKLVQKHPALR 130 L++ L+ Sbjct: 206 WCLLRDDKVLQ 216 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.135 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 356,044 Number of extensions: 11718 Number of successful extensions: 91 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 92 Number of HSP's successfully gapped: 2 Length of query: 150 Length of database: 1,619,870 Length adjustment: 77 Effective length of query: 73 Effective length of database: 1,255,275 Effective search space: 91635075 Effective search space used: 91635075 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 126 (53.1 bits)