BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0420.p (119 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309383|ref|YP_003773475.1| permease [Herbaspirillum serope... 162 9e-42 >gi|300309383|ref|YP_003773475.1| permease [Herbaspirillum seropedicae SmR1] Length = 412 Score = 162 bits (409), Expect = 9e-42, Method: Compositional matrix adjust. Identities = 94/119 (78%), Positives = 104/119 (87%) Query: 1 VTRLGIGLAILSFVAMASGPAFSASGQLWLLALAAIGFDLGVQATLVAHQTIVYSIEPGA 60 VTRLG+GLAI+SF AMA P FS GQL LLA+AA+GFDLGVQATLVAHQTIVYSIEPGA Sbjct: 294 VTRLGVGLAIVSFAAMALSPWFSTQGQLVLLAVAAVGFDLGVQATLVAHQTIVYSIEPGA 353 Query: 61 RSRLNAVLFVGMFVGMAAGAALGSLLLAQWGWIGVVGLATVASLSALLVRLLPGKNRDR 119 RSRLNA+LFVGMF+GMAAGAALGSL+LAQWGWI VV LAT+A+ AL+VRL PG R R Sbjct: 354 RSRLNAILFVGMFIGMAAGAALGSLVLAQWGWIAVVALATLAAGLALVVRLWPGAARAR 412 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.139 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 258,780 Number of extensions: 7556 Number of successful extensions: 58 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 58 Number of HSP's successfully gapped: 2 Length of query: 119 Length of database: 1,619,870 Length adjustment: 74 Effective length of query: 45 Effective length of database: 1,269,480 Effective search space: 57126600 Effective search space used: 57126600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 124 (52.4 bits)