BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0418.p (390 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314043|ref|YP_003778135.1| cobalamin synthesis protein/P47... 546 e-157 gi|300311859|ref|YP_003775951.1| CobW/P47K family protein [Herba... 134 2e-32 gi|300310263|ref|YP_003774355.1| cobalamin synthesis P47K protei... 117 2e-27 gi|300311960|ref|YP_003776052.1| G3E family GTPase [Herbaspirill... 64 1e-11 >gi|300314043|ref|YP_003778135.1| cobalamin synthesis protein/P47K family protein [Herbaspirillum seropedicae SmR1] Length = 388 Score = 546 bits (1408), Expect = e-157, Method: Compositional matrix adjust. Identities = 291/385 (75%), Positives = 324/385 (84%), Gaps = 14/385 (3%) Query: 11 DAPALIPISLLTGFLGSGKTTLLNHLVAQPELKDTLVIINEFGEIGLDHLLVAHSQDEVV 70 DAP IP+SLLTGFLGSGKTTLLNHL+ QPE+KDTLVIINEFGEIGLDHLLVAHSQ+E + Sbjct: 9 DAP--IPVSLLTGFLGSGKTTLLNHLITQPEMKDTLVIINEFGEIGLDHLLVAHSQEETI 66 Query: 71 VEMSSGCLCCTIRGDLKKTLKDITWRFAEGGKRKFNRVIIETTGLASPIPILHTLMTDNF 130 VEMSSGCLCCTIRGDLKKTLKDITWRFAEGG+RKFNRV+IETTGLASP PILHTLMTD F Sbjct: 67 VEMSSGCLCCTIRGDLKKTLKDITWRFAEGGRRKFNRVVIETTGLASPAPILHTLMTDAF 126 Query: 131 IASKYRLDGVITTVDAVNGNATLDNHIEAVQQAAVADRLLLTKSDLANAADPAALQDLQA 190 IA +YRLDGVI TVDAVNG +TLD+H EAVQQAAVADRLLLTK+DL +AA AA LQA Sbjct: 127 IAERYRLDGVIVTVDAVNGMSTLDHHEEAVQQAAVADRLLLTKTDLVDAATLAA---LQA 183 Query: 191 RLKQINPGAQQILSQQGRVAPQQLLDAGLFRTADKTPDVERWLSEEAYEP------PHDH 244 RL+ +NPGA+Q+ Q G++A +LLDAGLF+ +K PDV RWL+EEA+ H Sbjct: 184 RLRTLNPGARQLQPQVGQIAAAELLDAGLFKPGEKMPDVARWLNEEAFAEQARHGHGHHG 243 Query: 245 DHAHGHHHHGHDHGHKHGHAGHGHDINRHDDHIRAFCFSFDKPIEPHLFDEWLSLLVGFK 304 H HHHGHDHGH H H HD+NRHDDHIRAFCF+FD+PI+P LFDEWLSLLVGFK Sbjct: 244 HHHGHDHHHGHDHGHDHHGHDHEHDVNRHDDHIRAFCFTFDEPIDPVLFDEWLSLLVGFK 303 Query: 305 GQNILRIKGIVNLAGEDKPTVIHGVQHIFHPTVTLPAWPSEDRRTRIVFITKDIERATIE 364 G NILRIKGI+NL GE PTVIHGVQHIFHPT TLP WPSEDRR+RIVFIT+D+ERATIE Sbjct: 304 GPNILRIKGILNLKGEALPTVIHGVQHIFHPTATLPQWPSEDRRSRIVFITRDVERATIE 363 Query: 365 RSFTAFMQAQAIESEQRQTPDKKSA 389 RSF AFMQAQAIE E R+ KK+A Sbjct: 364 RSFDAFMQAQAIEREARE---KKAA 385 >gi|300311859|ref|YP_003775951.1| CobW/P47K family protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 134 bits (336), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 108/376 (28%), Positives = 176/376 (46%), Gaps = 84/376 (22%) Query: 10 QDAPALIPISLLTGFLGSGKTTLLNHLVAQPELKDTLVIINEFGEIGLDHLLVAHS---- 65 Q A +P+++L+GFLG+GKTTLLNH++ E + VI+N+ E+ +D LV Sbjct: 7 QAADRRLPVTVLSGFLGAGKTTLLNHILHNREGRRVAVIVNDMSEVNIDAALVRDGGADL 66 Query: 66 --QDEVVVEMSSGCLCCTIRGDLKKTLKDITWRFAEGGKRKFNRVIIETTGLASPIPILH 123 +E +VEMS+GC+CCT+R DL ++ + +R+F++++IE+TG++ P+P+ Sbjct: 67 ARTEEKLVEMSNGCICCTLREDLLMEVRKL------ARERRFDQLVIESTGISEPLPVAE 120 Query: 124 TL-MTDNFIAS---KYRLDGVITTVDAVN------GNATLDNHIEA-------------V 160 T TD AS RLD ++T VDA N +LD ++ + Sbjct: 121 TFTFTDEDGASLSDVARLDTMVTVVDAFNFLKDYSSQDSLDQRGQSMGEEDNRTVVDLLI 180 Query: 161 QQAAVADRLLLTKSDLANAADPAALQDLQARLKQINPGAQQILSQQGRVAPQQLLDAGLF 220 +Q D ++L K DL A D L+ L A L ++NP A+ + ++ G+VA +Q+LD GLF Sbjct: 181 EQIEFCDVIVLNKLDLVAADD---LERLHAILHKLNPRARIVRAEFGKVALEQVLDTGLF 237 Query: 221 RTADKTPDVERWLSEEAYEPPHDHDHAHGHHHHGHDHGHKHGHAGHGHDINRHDDHIRAF 280 E+A + P G H ++G I +F Sbjct: 238 DF------------EQASQAPSWLQELRGEHVP---ETEQYG--------------ISSF 268 Query: 281 CFSFDKPIEPHLFDEWLSLLVGFKGQNILRIKGIVNLAGEDKPT-----------VIHGV 329 + +P+ P F + L+ + ++R KG LA +PT HG+ Sbjct: 269 AWQARRPLHPQRFFD----LINTEWPGVVRSKGFFWLAS--RPTQAGSWSQAGAVCRHGL 322 Query: 330 QHIFHPTVTLPAWPSE 345 + V WP + Sbjct: 323 AGKWWAAVPREQWPQD 338 >gi|300310263|ref|YP_003774355.1| cobalamin synthesis P47K protein [Herbaspirillum seropedicae SmR1] Length = 413 Score = 117 bits (292), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 84/256 (32%), Positives = 135/256 (52%), Gaps = 37/256 (14%) Query: 16 IPISLLTGFLGSGKTTLLNHLVAQPELKDTLVIINEFGEIGLD------HLLVAHSQDEV 69 IP+++L+GFLGSGKTTLLNH++ VI+N+ E+ +D + + DE Sbjct: 17 IPVTVLSGFLGSGKTTLLNHILRNRAGMKVAVIVNDMSEVNMDGDDVRRNTELYRGNDE- 75 Query: 70 VVEMSSGCLCCTIRGDLKKTLKDITWRFAEGGKRKFNRVIIETTGLASPIPILHT---LM 126 +VEMS+GC+CCT+R DL + + + A G +F+ ++IE+TG++ PIP+ T L Sbjct: 76 LVEMSNGCICCTLRADLLEQVSAL----ARSG--RFDYLLIESTGISEPIPVAETFAFLD 129 Query: 127 TDNFIASKY-RLDGVITTVDAVNGNATLDNHIEAVQQAAVADR----LLLTKSDLANA-- 179 +D F S+ RLD ++T V+ L +H+ Q + R LL+ + + AN Sbjct: 130 SDGFSLSELARLDTLVTVVNGETFEQQLADHVAIAQADGASTRPLSDLLIEQVEYANVIL 189 Query: 180 ------ADPAALQDLQARLKQINPGAQQILSQQGRVAPQQLLDAGLFRTADKTPDVER-- 231 D +A L+A L+++NP A+ + G V Q+LD LF P + R Sbjct: 190 VSRVDLIDASAFASLKAVLQKLNPTARILPMAHGEVDLDQVLDTRLF----DLPSLARMP 245 Query: 232 -WL-SEEAYEPPHDHD 245 W+ + + PP + D Sbjct: 246 GWMQTMDGAHPPSEAD 261 >gi|300311960|ref|YP_003776052.1| G3E family GTPase [Herbaspirillum seropedicae SmR1] Length = 377 Score = 64.3 bits (155), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 47/144 (32%), Positives = 78/144 (54%), Gaps = 6/144 (4%) Query: 5 GLAMTQDAPAL--IPISLLTGFLGSGKTTLLNHLVAQPELKDTLVIINEFGEIGLD-HLL 61 L++ + APAL IP++++TGFLG+GKTTLL L+ + + + ++INEFGE+ +D LL Sbjct: 4 ALSLPELAPALPPIPVTVVTGFLGAGKTTLLRGLLQRRQSRRLALLINEFGEVAIDGDLL 63 Query: 62 VAHSQDEVVVEMSSGCLCCTIRGDLKKTLKDITWRFAEGGKRKFNRVIIETTGLASPIPI 121 A + V++ GD + + A + + + V+IET+GLA P + Sbjct: 64 RAEGDAQGQVQIQDFAHGLIAYGDDAHFVPAMLALAAR--RAQIDHVLIETSGLALPSAV 121 Query: 122 LHTLMTDNFIASKYRLDGVITTVD 145 + L +A + LD + VD Sbjct: 122 MEVLQGAE-MARHFVLDATLAVVD 144 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,080,992 Number of extensions: 39854 Number of successful extensions: 150 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 140 Number of HSP's successfully gapped: 4 Length of query: 390 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 303 Effective length of database: 1,207,925 Effective search space: 366001275 Effective search space used: 366001275 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)