BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0400.p (263 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312114|ref|YP_003776206.1| 3-oxoacyl-[acyl-carrier-protein... 132 3e-32 gi|300313237|ref|YP_003777329.1| dehydrogenase [Herbaspirillum s... 110 2e-25 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 109 2e-25 gi|300313745|ref|YP_003777837.1| short-chain alcohol dehydrogena... 106 2e-24 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 106 2e-24 >gi|300312114|ref|YP_003776206.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 262 Score = 132 bits (332), Expect = 3e-32, Method: Compositional matrix adjust. Identities = 89/269 (33%), Positives = 143/269 (53%), Gaps = 13/269 (4%) Query: 1 MDLQLKGKTALVSGSTAGIGYAIAHTLAREGANVIVNGRTQASVDEAAERIRSE---TNG 57 MDL+L GKTALV S+ G+G A AH+LAREG +V + R + +++ AA+ IR+E T Sbjct: 1 MDLRLNGKTALVCASSKGLGRACAHSLAREGVHVTMLARGREALEAAAQEIRAEVAATGA 60 Query: 58 TVQGYAGDLSKPDAANEVIQRHPGINILVNNLGIFEPKAFEDIPDEDWLRFFDVNVLSGV 117 + + D++ P+ + P +ILV N G +P F D EDW+ D N+L+ + Sbjct: 61 IITTVSCDITTPEGRALALAACPAPDILVTNAGGPKPGDFRDWSREDWIAAVDANMLTPI 120 Query: 118 RLARLVLPEMKRTNWGRIIFISSESAVQIPTEMIHYGMTKTAQLAVS---RGLAESVAGT 174 L + + M +GRI+ I+S SAV+ P +++ G++ A+ ++ GLA Sbjct: 121 ELIKATVDGMIARRFGRIVNITS-SAVRAPIDIL--GLSNGARSGLTGFVAGLARKTVAH 177 Query: 175 GITVNSILPGPTKSRGVGDFVEDMARASDKTFEQVEAEFFDHVRPTSLIKRFGTPQEVAS 234 +T+N++LPGP ++ + A+ S ++ +++ AE R + RFG P E Sbjct: 178 NVTINNLLPGPFETDRLRATASGAAQESGRSVDEILAER----RKLNPAGRFGDPAEFGD 233 Query: 235 LVAYVVSPLASATTGAALRVDGGVIKSAF 263 AY+ S A TG L +DGG F Sbjct: 234 ACAYLCSAQAGFITGQNLLMDGGAYPGTF 262 >gi|300313237|ref|YP_003777329.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 264 Score = 110 bits (274), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 86/275 (31%), Positives = 139/275 (50%), Gaps = 24/275 (8%) Query: 1 MDLQLKGKTALVSGSTAGIGYAIAHTLAREGANVIVNGRTQASVDEAAERIRSETNGTVQ 60 M+L+L+GK A+V+G + GIG A+A LAREG V + R++ ++E A + T V Sbjct: 1 MELKLQGKAAVVTGGSLGIGRAVAEALAREGVRVAIVARSKGKLEEVASALSQSTGTEVI 60 Query: 61 GYAGDLSKP---DAANEVIQRHPG-INILVNNLGIFEPKAF-----EDIPDEDWLRFFDV 111 A D+S +AA +H G I+ILVN G P + E L ++ Sbjct: 61 AVAADVSNTEQVEAAVAQAAQHFGRIDILVN--GAAHPGGLVRSEIQHASPEGLLEDINI 118 Query: 112 NVLSGVRLARLVLPEMKRTNWGRIIFI---SSESAVQIPTEMIHYGMTKTAQLAVSRGLA 168 V+ +R A+ V P M+ +GRII I + + Q+ GM A +++ L+ Sbjct: 119 KVVGYMRFAKAVAPHMRAGGYGRIINIGGLTGRGSKQLS------GMRNVAICHLTKTLS 172 Query: 169 ESVAGTGITVNSILPGPTKSRGVGDFVEDMARASDKTFEQVEAEFFDHVRPTSLIKRFGT 228 + + GITVN I PG ++ + + E A+ T EQVE + + I+R Sbjct: 173 DQLGPDGITVNVIHPGVVETPHIHELYEKEAKLQGLTPEQVEQNYAK----ATPIRRVLQ 228 Query: 229 PQEVASLVAYVVSPLASATTGAALRVDGGVIKSAF 263 +E+A +V ++ SP A+A TG ++ VDGG+ + + Sbjct: 229 VEEIADVVLFLASPRAAAITGESIAVDGGITRGIY 263 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 109 bits (273), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 84/260 (32%), Positives = 132/260 (50%), Gaps = 27/260 (10%) Query: 8 KTALVSGSTAGIGYAIAHTLAREGANVIVNGRTQASVDEAAERIRSETNGTVQGYAGDLS 67 + ALV+G GIG +I+ LA +G V+V + + A+ + + + DL Sbjct: 9 RVALVTGGAMGIGASISQQLAAKGHTVLVADINLDAASKTADALVAAGHRAAP-LQMDLG 67 Query: 68 KPD---AANEVIQRHPG-INILVNNLGIFEPKAFEDIPDEDWLRFFDVNV----LSGVRL 119 P+ AA I++ G ++LVNN G+ +F D P E+WL+ +VNV L+G Sbjct: 68 SPESIAAAFAHIEKDYGRCDVLVNNAGVARTYSFLDYPLENWLQTMNVNVTGVLLAGQHA 127 Query: 120 ARLVLPEMKRTNWGRIIFISSESAVQIPTEMIHYGMTKTAQLAVSRGLAESVAGTGITVN 179 ARL M + WGRI+ ISS S ++ YG +K A + ++R +A +A GITVN Sbjct: 128 ARL----MVKQQWGRIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQYGITVN 183 Query: 180 SILPGPTKSRGVGDFVEDMARASDKTFEQVEAEFFDHVRPTSLIKRFGTPQEVASLVAYV 239 S+ PGP + M +A EQ ++ V ++R+GT E+AS V Y+ Sbjct: 184 SVAPGPVDT--------PMTQAMHS--EQTRESYYRLVP----MRRYGTTDEIASAVCYL 229 Query: 240 VSPLASATTGAALRVDGGVI 259 + ++ TG + VDGG + Sbjct: 230 AAEESAYITGHVIPVDGGFV 249 >gi|300313745|ref|YP_003777837.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 106 bits (264), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 85/261 (32%), Positives = 124/261 (47%), Gaps = 14/261 (5%) Query: 1 MDLQLKGKTALVSGSTAGIGYAIAHTLAREGANVIVNGRTQASVDEAAERIRSETNGTVQ 60 M+ QL GK ALV+G T+GIG A A LA +GA V + GR QA +D A I + G ++ Sbjct: 1 MNKQLNGKIALVTGGTSGIGLATAQELAAQGAQVFITGRRQAELDAAVASIGAAATG-IR 59 Query: 61 GYAGDLSKPDAANEVIQRHPG-INILVNNLGIFEPKAFEDIPDEDWLRFFDVNVLSGVRL 119 A LS+ D I + G ++IL N G + I +E + R F NV + Sbjct: 60 ADASVLSELDTVYAQIAKSAGRLDILFANAGGGDMMPLGAITEEHFDRIFGTNVRGVLFT 119 Query: 120 ARLVLPEMKRTNWGRIIFISSESAVQIPTEMIHYGMTKTAQLAVSRGLAESVAGTGITVN 179 + LP + + +I SS ++V Y +K A +R A + I VN Sbjct: 120 VQKALPLL--VDGASVILTSSTTSVLGTANFSVYSASKAAVRNFARSWALDLKDRAIRVN 177 Query: 180 SILPGPTKSRGVGDFVEDMARASDKTFEQVEAEFFDHVRPTSLIKRFGTPQEVASLVAYV 239 + PGP ++ G+G+ V D AR FD++ + R G PQE+ VA++ Sbjct: 178 VVSPGPIRTPGLGELVPDDARQG----------LFDYLASQVPLGRLGEPQEIGKAVAFL 227 Query: 240 VSPLASATTGAALRVDGGVIK 260 S AS G L VDGG+ + Sbjct: 228 ASDAASFVNGIELFVDGGMAQ 248 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 106 bits (264), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 87/261 (33%), Positives = 129/261 (49%), Gaps = 25/261 (9%) Query: 5 LKGKTALVSGSTAGIGYAIAHTLAREGANVIVNGRTQASVDEAAERIRSETNGTVQGYAG 64 L+G+ ALV+G++ GIG AIA LAR+GA VI +++ E + + G G Sbjct: 6 LQGQVALVTGASRGIGRAIATELARQGAKVIGTATSESGAAAITEYLAAL--GPEAGRGA 63 Query: 65 DLSKPDAANEV-----IQRHPG-INILVNNLGIFEPKAFEDIPDEDWLRFFDVNVLSGVR 118 L+ DA V +Q+ G ++ILVNN GI + + + DE+W N+ + R Sbjct: 64 VLNVNDAEASVALVEQVQKDFGSLSILVNNAGITQDQLAMRMKDEEWDSVIATNLTAVGR 123 Query: 119 LARLVLPEMKRTNWGRIIFISSESAVQIPTEMIHYGMTKTAQLAVSRGLAESVAGTGITV 178 L+R VL M + GRII I+S ++Y K +SR LA + ITV Sbjct: 124 LSRAVLRGMMKARTGRIINITSVVGEAGNPGQMNYAAAKAGVAGMSRALAREIGSRNITV 183 Query: 179 NSILPGPTKSRGVGDFVE-DMARASDKTFEQVEAEFFDHVRPTSLIKRFGTPQEVASLVA 237 N + PG F++ DM RA + EQ A + P + R G +E+A VA Sbjct: 184 NCVAPG---------FIDTDMTRALN---EQQSAAILQQI-P---LGRLGAAEEIAYAVA 227 Query: 238 YVVSPLASATTGAALRVDGGV 258 ++ SP A+ TG L V+GG+ Sbjct: 228 FLASPQAAYITGTTLNVNGGM 248 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.133 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 807,557 Number of extensions: 32428 Number of successful extensions: 250 Number of sequences better than 1.0e-08: 45 Number of HSP's gapped: 189 Number of HSP's successfully gapped: 45 Length of query: 263 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 180 Effective length of database: 1,226,865 Effective search space: 220835700 Effective search space used: 220835700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 129 (54.3 bits)