BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0390.p (265 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314033|ref|YP_003778125.1| acyl-CoA dehydrogenase [Herbasp... 275 2e-75 gi|300314032|ref|YP_003778124.1| acyl-CoA dehydrogenase [Herbasp... 230 8e-62 gi|300310289|ref|YP_003774381.1| dibenzothiophene desulfurizatio... 216 1e-57 >gi|300314033|ref|YP_003778125.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 411 Score = 275 bits (704), Expect = 2e-75, Method: Compositional matrix adjust. Identities = 167/272 (61%), Positives = 202/272 (74%), Gaps = 23/272 (8%) Query: 1 VLAQTATESQKHYFYSEVLNGARFGNAGPERTSKNVLDIRTRITKDPGSPD--YLLNGTR 58 ++A+ A++ Q+ F++EVL+GAR GNAGPER KNVLD+RTR T D + D YLLNGTR Sbjct: 109 LIARLASQEQQRLFFAEVLDGARLGNAGPERNGKNVLDVRTRATLDRAAGDGSYLLNGTR 168 Query: 59 FYSTGALYAX---------NG------TRFYSTGA------SGFGQRTTASGTVIFDNVR 97 FYSTGALYA +G TR + G S FGQRTTASGTV+FDNVR Sbjct: 169 FYSTGALYAHWIPSRAIDDDGQPLVVYTRRDAPGVRVIDDWSSFGQRTTASGTVVFDNVR 228 Query: 98 VSAAHVVPLHQLADQPNNIGPVAQLIQAAIDAGIAQAAVEDAVAFIRQRSRPWADSGVER 157 V A V+ L QPNNIGPV+QLIQAAIDAGIA AAV+D +AF+ RSRP+ DSG+ER Sbjct: 229 VPATQVLALKDALQQPNNIGPVSQLIQAAIDAGIAHAAVQDTIAFVTTRSRPYVDSGLER 288 Query: 158 AGDDPLTINQIGDLKIRLHAAEALLERAARTLDDLPLPISYEQMAQASVAVAEAKVLTTE 217 A DDP + IG+L +RLHAA+ALLE AA LD LP +S+EQMAQAS AVAEAKVL+TE Sbjct: 289 ASDDPYILRDIGNLSLRLHAADALLEDAAALLDSLPAQLSFEQMAQASAAVAEAKVLSTE 348 Query: 218 IALHASEKLFELAGTQSTLSAHDLDRHWRNAR 249 +AL ASEKLFEL+G+ +TL++H+LDRHWRNAR Sbjct: 349 LALLASEKLFELSGSAATLASHNLDRHWRNAR 380 >gi|300314032|ref|YP_003778124.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 421 Score = 230 bits (587), Expect = 8e-62, Method: Compositional matrix adjust. Identities = 133/265 (50%), Positives = 172/265 (64%), Gaps = 21/265 (7%) Query: 6 ATESQKHYFYSEVLNGARFGNAGPERTSKNVLDIRTRITKDPGSPDYLLNGTRFYSTGAL 65 +ESQK +F+ VL G RFGNA E +K D +TR++++ P Y ++G +FY+TGAL Sbjct: 126 GSESQKRFFFERVLQGDRFGNALAELGTKTSHDRKTRLSRNAHGPGYRIDGRKFYATGAL 185 Query: 66 YAX---------NGTRFYST------------GASGFGQRTTASGTVIFDNVRVSAAHVV 104 YA +G + + SGFGQRTT SG+ +F+NV V A V+ Sbjct: 186 YADWVPTSVIDDDGVQQLAIVPRHAPGLTIIDDWSGFGQRTTGSGSAVFENVHVPADAVI 245 Query: 105 PLHQLADQPNNIGPVAQLIQAAIDAGIAQAAVEDAVAFIRQRSRPWADSGVERAGDDPLT 164 +P IGP+AQ+ AAID GIA+AA D + F+R RSR W DSGVERA DDPLT Sbjct: 246 GFATAFSRPTQIGPMAQIQHAAIDLGIARAAYADLLTFVRTRSRAWVDSGVERAVDDPLT 305 Query: 165 INQIGDLKIRLHAAEALLERAARTLDDLPLPISYEQMAQASVAVAEAKVLTTEIALHASE 224 I ++GDL I+LHAAEALL RA R +D + +A AS+AVAEA+VLTTE++L A Sbjct: 306 IREVGDLTIQLHAAEALLRRAGRKVDAAAAAPDEDSVAAASIAVAEARVLTTEVSLAAGS 365 Query: 225 KLFELAGTQSTLSAHDLDRHWRNAR 249 KLFELAG+QSTL+ H+LDRHWRNAR Sbjct: 366 KLFELAGSQSTLAEHNLDRHWRNAR 390 >gi|300310289|ref|YP_003774381.1| dibenzothiophene desulfurization flavin reductase [Herbaspirillum seropedicae SmR1] Length = 419 Score = 216 bits (551), Expect = 1e-57, Method: Compositional matrix adjust. Identities = 122/261 (46%), Positives = 160/261 (61%), Gaps = 23/261 (8%) Query: 10 QKHYFYSEVLNGARFGNAGPERTSKNVLDIRTRITKDPGSPDYLLNGTRFYSTGALYA-- 67 Q+ F+++ L G R+GNA E SK D TR D G ++ G +FYSTGAL A Sbjct: 130 QQRRFFADFLRGVRWGNAFSESGSKRAADFETRFV-DAGD-HVIVTGKKFYSTGALLAHY 187 Query: 68 --------XNGTRFYST-----------GASGFGQRTTASGTVIFDNVRVSAAHVVPLHQ 108 N T + S FGQRTT SGTVI D V+V H++P ++ Sbjct: 188 VPIVANDEENRTWYVVAERNAPGLTVIDDWSAFGQRTTLSGTVIIDQVKVPKTHLIPAYK 247 Query: 109 LADQPNNIGPVAQLIQAAIDAGIAQAAVEDAVAFIRQRSRPWADSGVERAGDDPLTINQI 168 +P+ G + Q+IQAA+D GIA+AA+ D F+R +SRPW DS + A +DP +I I Sbjct: 248 GYLEPSADGAIFQIIQAAVDVGIARAAIADTQDFVRTKSRPWIDSQRDHAWEDPYSIQAI 307 Query: 169 GDLKIRLHAAEALLERAARTLDDLPLPISYEQMAQASVAVAEAKVLTTEIALHASEKLFE 228 GDL+IRLHA+EALLERA R +D + +A A + AEAKVL+TEIA+HA+ KLFE Sbjct: 308 GDLQIRLHASEALLERAGRAIDAAVAAPDAQSVAHAQILTAEAKVLSTEIAIHATNKLFE 367 Query: 229 LAGTQSTLSAHDLDRHWRNAR 249 LAGT+STL ++LDRHWRNAR Sbjct: 368 LAGTRSTLGQYNLDRHWRNAR 388 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.130 0.369 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 612,730 Number of extensions: 20475 Number of successful extensions: 92 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 86 Number of HSP's successfully gapped: 3 Length of query: 265 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 181 Effective length of database: 1,222,130 Effective search space: 221205530 Effective search space used: 221205530 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)