BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0376.p (464 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314031|ref|YP_003778123.1| coenzyme F420-dependent N5,N10-... 781 0.0 gi|300313871|ref|YP_003777963.1| thiophene oxidase [Herbaspirill... 265 9e-72 gi|300310284|ref|YP_003774376.1| coenzyme F420-dependent N5,N10-... 89 7e-19 gi|300310902|ref|YP_003774994.1| alkanesulfonate monooxygenase [... 74 2e-14 >gi|300314031|ref|YP_003778123.1| coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase [Herbaspirillum seropedicae SmR1] Length = 459 Score = 781 bits (2017), Expect = 0.0, Method: Compositional matrix adjust. Identities = 369/451 (81%), Positives = 405/451 (89%), Gaps = 1/451 (0%) Query: 6 KKEIRLNAFNMNCVNHINHGLWTHPRDRSQDYTDIAYWTDLAKLLERGKFDGLFLADIVG 65 KKEIRLNAFNMNCV+HINHGLWTHPRDRSQDYTDIAYWTDLA+LLERGKFDGLFLADIVG Sbjct: 3 KKEIRLNAFNMNCVSHINHGLWTHPRDRSQDYTDIAYWTDLARLLERGKFDGLFLADIVG 62 Query: 66 VYDVYQNSVDITLRESIQLPVNDPLLLISAMGFVTEHLGFGITANLTYEAPFLFARRMST 125 VYDVYQ + D+TLRE+IQLPVNDPL+L+ AM VTEHLGFG+TANLTYEAPFLFARRMST Sbjct: 63 VYDVYQGNTDLTLRETIQLPVNDPLMLVPAMAAVTEHLGFGVTANLTYEAPFLFARRMST 122 Query: 126 LDHLTKGRVGWNIVTGYLDSAARAMGLSEQLEHDQRYDRADEFMEVVYKLWEGSWEDDAV 185 LDHL++GRVGWNIVTGYLDSAARAMGL EQLEHDQRYDRADEFMEVVYKLWEGSWED AV Sbjct: 123 LDHLSRGRVGWNIVTGYLDSAARAMGLHEQLEHDQRYDRADEFMEVVYKLWEGSWEDGAV 182 Query: 186 IRDRQNRIYAQPDKVHKIKHSGDYYKVEGYHLSEPSRQRTPVLYQAGSSGRGQAFGARHA 245 +RDR R+YA P KVHKI H GDYY VEG HLSEPSRQRTPVLYQAGSSGRGQAFGARHA Sbjct: 183 LRDRARRVYADPAKVHKIAHHGDYYHVEGIHLSEPSRQRTPVLYQAGSSGRGQAFGARHA 242 Query: 246 ECVFVSGQSKEAVRKLVTGIREGAVREGRQADDVKIFMGLTVVVAPTEKEAREKYAEYAR 305 ECVF+S QSK+A RKLV IR+GAVREGR D +KIFMGLTVV TE++A+E+Y EYAR Sbjct: 243 ECVFISSQSKDATRKLVQSIRDGAVREGRDPDAIKIFMGLTVVTGRTEQQAQERYREYAR 302 Query: 306 YASPEAGLAHFSSGTGIDFSQYELDEPIRQVKTNSIESLVKTVTNPELNWTKRKLLEQLE 365 YASPEAGLAH+S+GTGIDFSQYELDEPIR KTN+IESL K VT PELNWTKRKLL+QLE Sbjct: 303 YASPEAGLAHYSAGTGIDFSQYELDEPIRPAKTNAIESLTKVVTQPELNWTKRKLLQQLE 362 Query: 366 LGGRYATIIGSPQQVADELEAWIRETDIDGFNLTRTVTPESFEAFVDLVIPELQTRGSYK 425 LGGRYA+I+G+P +VADELE+W+ +T IDG+NLTRTVTPESFE FV+LVIP LQ RGSYK Sbjct: 363 LGGRYASIVGAPDRVADELESWVADTGIDGYNLTRTVTPESFEDFVELVIPVLQERGSYK 422 Query: 426 HDYAPGTLREKLFTGSGARLPQQHTGARYRT 456 +YA GTLREKLF GA LPQ+H GA +R Sbjct: 423 REYAQGTLREKLFQ-RGATLPQEHVGAGFRV 452 >gi|300313871|ref|YP_003777963.1| thiophene oxidase [Herbaspirillum seropedicae SmR1] Length = 433 Score = 265 bits (676), Expect = 9e-72, Method: Compositional matrix adjust. Identities = 165/439 (37%), Positives = 235/439 (53%), Gaps = 20/439 (4%) Query: 9 IRLNAFNMNCVNHINHGLWTHPRDRSQDYTDIAYWTDLAKLLERGKFDGLFLADIVGVYD 68 + L AF + + LW HP+ R + ++ Y+ +A+ LERGKFD LF AD + V Sbjct: 1 MHLAAFLIAGNAAHSQALWRHPQSRRGGFLELDYYLHIARTLERGKFDLLFFADRLAVST 60 Query: 69 VYQNS----VDITLRESIQLPVNDPLLLISAMGFVTEHLGFGITANLTYEAPFLFARRMS 124 Y +S + I +++ +L DPL ++ A+ T H+G G T + TY P+ AR + Sbjct: 61 RYGDSHLHGLYIGDQDATRL---DPLPILGALAAATSHIGLGATRSTTYSQPYSLAREFA 117 Query: 125 TLDHLTKGRVGWNIVTGYLDSAARAMGLSEQLEHDQRYDRADEFMEVVYKLWEGSWEDDA 184 TLDHL+ GR WN+VT A GL E L HD RYDRADEF+EV +KLW SW+ DA Sbjct: 118 TLDHLSGGRAAWNVVTSVNQGEADNFGLKETLPHDVRYDRADEFLEVTHKLWH-SWQPDA 176 Query: 185 VIRDRQNRIYAQPDKVHKIKHSGDYYKVEGYHLSEPSRQRTPVLYQAGSSGRGQAFGARH 244 +R +Q+R YA DKV I H G ++ V G S Q PV+ QAGSS RGQ F AR Sbjct: 177 -LRLQQDR-YADADKVAAIDHQGAHFSVRGPLNIPASPQGHPVIIQAGSSDRGQDFAARW 234 Query: 245 AECVFVSGQSKEAVRKLVTGIREGAVREGRQADDVKIFMGLTVVVAPTEKEAREKYAEYA 304 AE VF ++ ++ A + GR+ D++I + V + + ARE + Sbjct: 235 AEVVFNIQPDLPRLQAFYRDLKNRAQQFGRRPQDIRILSAVMPFVGQSRQHARELQEQAN 294 Query: 305 RYASPEAGLAHFSSGTGIDFSQYELDEPIRQVKTNSIESLVKTVTNPELNWTKRKLLEQL 364 A P GL+ SS +DFS + LD P+ V+ + I+ L K + + + L Sbjct: 295 ALADPLVGLSTLSSHMNVDFSGFALDAPLGDVQVSGIQGLFKLLR----AISAERQLTLA 350 Query: 365 ELGGRYATIIGSPQ------QVADELEAWIRETDIDGFNLTRTVTPESFEAFVDLVIPEL 418 + G YA + +PQ VAD L++ I DGF ++ + P F+ FVD VIPEL Sbjct: 351 DAGRLYAQGVLTPQIAGTAADVADWLQSIIVANGADGFVISPSHLPYGFDDFVDQVIPEL 410 Query: 419 QTRGSYKHDYAPGTLREKL 437 Q RG+++ DYA LR+ L Sbjct: 411 QRRGAFRQDYAGRQLRQHL 429 >gi|300310284|ref|YP_003774376.1| coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase [Herbaspirillum seropedicae SmR1] Length = 385 Score = 89.0 bits (219), Expect = 7e-19, Method: Compositional matrix adjust. Identities = 68/273 (24%), Positives = 128/273 (46%), Gaps = 50/273 (18%) Query: 116 PFLFARRMSTLDHLTKGRVGWNIVTGYLDSAARAMGLSEQLEHDQRYDRADEFMEVVYKL 175 P + A++++T+DH++ GR+ N+V+G+ +A+G LEHD+RY R++EF+ + + Sbjct: 106 PAVVAKQIATIDHISNGRIAINVVSGWFKGEFQAIG-EPWLEHDERYRRSNEFIRALKGI 164 Query: 176 WEGSWEDDAVIRDRQNRIYAQPDKVHKIKHSGDYYKVEGYHLS-EPSRQRTPVLYQAGSS 234 W +DD + GD+Y+ Y LS +P ++ P ++Q GSS Sbjct: 165 WT---QDDFTFK-------------------GDFYRFNNYTLSPKPVQKPHPEIFQGGSS 202 Query: 235 GRGQAFGARHAECVFVSGQSKEAVRKLVTGIREGAVREGRQADDVKIFMGLTVVVAPTEK 294 + A ++ F +G + E ++ + I+ A + G + V+I + V+ TE+ Sbjct: 203 RAARDNAASVSDWYFTNGNTLEGLKAQIDDIQAKAAQNGHK---VRIGVNAFVIARDTEE 259 Query: 295 EAREKYAEYARYASPEAGLAHFSSGTGIDFSQYELDEPIRQVKTNSIESLVKTVTNPELN 354 EA+ + +A EA + + ++Q S E E N Sbjct: 260 EAQAVLQDIIDHAHVEA--------------VHAFGDAVKQAGKASPEG--------EGN 297 Query: 355 WTKRKLLEQLELGGRYAT-IIGSPQQVADELEA 386 W K + ++ + T +IG+PQQ+A+ + A Sbjct: 298 WAKSTFEDLVQYNDGFKTNLIGTPQQIAERIVA 330 >gi|300310902|ref|YP_003774994.1| alkanesulfonate monooxygenase [Herbaspirillum seropedicae SmR1] Length = 375 Score = 74.3 bits (181), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 56/210 (26%), Positives = 96/210 (45%), Gaps = 28/210 (13%) Query: 88 DPLLLISAMGFVTEHLGFGITANLTYEAPFLFARRMSTLDHLTKGRVGWNIVTGYLDSAA 147 DP ++ +++ VT+ + + + P AR+ +TLD GR+ N+V G + Sbjct: 57 DPWVVAASLVPVTQRIKLLVALRTSLGGPGPSARQAATLDQALNGRLLLNVVPGGDATEL 116 Query: 148 RAMGLSEQLEHDQRYDRADEFMEVVYKLWEGSWEDDAVIRDRQNRIYAQPDKVHKIKHSG 207 A G+ L+HD+RY+ ADE++ + KL +G + + G Sbjct: 117 EADGVF--LKHDERYEAADEYLTIWRKLLQG----------------------ETVNYEG 152 Query: 208 DYYKVEGYHLSEPSRQRT-PVLYQAGSSGRGQAFGARHAECVFVSGQSKEAVRKLVTGIR 266 ++++ L P+ Q+ P LY GSS A+H + G+ AVR+ + +R Sbjct: 153 KHFRIRNGQLFHPAVQQPYPPLYFGGSSPAAHDLAAKHVDAYLTWGEPPAAVRQKIDDVR 212 Query: 267 EGAVREGRQADDVKIFMGLTVVVAPTEKEA 296 E A R+GR V+ + L +V T EA Sbjct: 213 ERAARQGRT---VRFGVRLHAIVRETTAEA 239 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,471,754 Number of extensions: 61992 Number of successful extensions: 158 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 151 Number of HSP's successfully gapped: 5 Length of query: 464 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 375 Effective length of database: 1,198,455 Effective search space: 449420625 Effective search space used: 449420625 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)