BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0373.p (288 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309401|ref|YP_003773493.1| nitrate/sulfonate/bicarbonate A... 469 e-133 gi|300311840|ref|YP_003775932.1| nitrate/sulfonate/bicarbonate A... 322 2e-89 gi|300309716|ref|YP_003773808.1| nitrate/sulfonate/bicarbonate A... 98 9e-22 gi|300310979|ref|YP_003775071.1| alkanesulfonates ABC transporte... 86 5e-18 gi|300309985|ref|YP_003774077.1| nitrate/sulfonate/bicarbonate A... 84 2e-17 >gi|300309401|ref|YP_003773493.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 288 Score = 469 bits (1207), Expect = e-133, Method: Compositional matrix adjust. Identities = 234/288 (81%), Positives = 261/288 (90%) Query: 1 MSISPPIRDEYEIALTPLDDVPLERTLSLPQRIWQQSWVRKAAILLVLALIWEIIARIQD 60 M+ +PPIRDEYEI LTPL + L+R SLPQR+WQQ WVRK AILL+LAL+WE +AR Q+ Sbjct: 1 MAQTPPIRDEYEIPLTPLPGLVLQRQFSLPQRLWQQGWVRKGAILLLLALVWEGVARWQN 60 Query: 61 NDLLLPSFAQTAHAFADGVITGELIGKVWISLKVLVKGYLIGIALAFALTTLAVSTQLGR 120 NDLLLPSF QTA AF DG+ +GEL GK W+SL+VL+K Y++GI AFALTTLAVSTQ GR Sbjct: 61 NDLLLPSFLQTAQAFIDGLRSGELFGKTWLSLQVLLKAYVLGIVAAFALTTLAVSTQFGR 120 Query: 121 DLLSTLTSMFNPLPAIAMLPLALLWFGLGENSLIFVLVHSVLWALALNTYAGFLGVSDTL 180 DLL TLTSMFNPLPAIA+LP+ALLWFGLG+ SLIFVLVHSVLWALA+NTYAGFLGVSDTL Sbjct: 121 DLLGTLTSMFNPLPAIALLPIALLWFGLGQGSLIFVLVHSVLWALAVNTYAGFLGVSDTL 180 Query: 181 RMAGRNYGLRGLRFVLFMLVPAALPSILAGLRIGWAFAWRTLIAAELVFGASSGKGGLGW 240 RMAGRNYGLRGL FVL +LVPAALPSILAGLRIGWAFAWRTLIAAELVFGA+SGKGGLGW Sbjct: 181 RMAGRNYGLRGLGFVLQVLVPAALPSILAGLRIGWAFAWRTLIAAELVFGATSGKGGLGW 240 Query: 241 YIFQNRNELYTDKVFAGLAMVILIGLVVENFGFNTIEKLTVKRWGMQR 288 YIFQNRNELYTDKVFAGLAMVILIGL+VEN FNT+E++TVK+WGMQR Sbjct: 241 YIFQNRNELYTDKVFAGLAMVILIGLLVENLVFNTLERVTVKKWGMQR 288 >gi|300311840|ref|YP_003775932.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 289 Score = 322 bits (826), Expect = 2e-89, Method: Compositional matrix adjust. Identities = 170/284 (59%), Positives = 213/284 (75%) Query: 5 PPIRDEYEIALTPLDDVPLERTLSLPQRIWQQSWVRKAAILLVLALIWEIIARIQDNDLL 64 P +R E + P ++R LS + +++ W+RK ILLVLA IWE+ R DN LL Sbjct: 6 PFVRPEILVDPNPHATAEVQRPLSAFESLYRMGWLRKTVILLVLAAIWEVYGRWLDNALL 65 Query: 65 LPSFAQTAHAFADGVITGELIGKVWISLKVLVKGYLIGIALAFALTTLAVSTQLGRDLLS 124 PSF++T AF G+ +G L+ + +S++ L+ GY +GI LA LTT+A+S+++G DLL Sbjct: 66 FPSFSETVQAFLSGIGSGVLLQRAAVSMQTLLVGYGLGILLAGLLTTVAISSRIGNDLLE 125 Query: 125 TLTSMFNPLPAIAMLPLALLWFGLGENSLIFVLVHSVLWALALNTYAGFLGVSDTLRMAG 184 TLTSMFNPLPAIA+LPLAL+WFGLG S+IFVLVHSVLWA+ALNT+ GF VS+TLRM G Sbjct: 126 TLTSMFNPLPAIALLPLALIWFGLGTGSIIFVLVHSVLWAVALNTHGGFRAVSNTLRMVG 185 Query: 185 RNYGLRGLRFVLFMLVPAALPSILAGLRIGWAFAWRTLIAAELVFGASSGKGGLGWYIFQ 244 +NYGLR V +L+PAA PSIL GL++GWAFAWRTLIAAELVFG SSG GGLGWYIF+ Sbjct: 186 QNYGLRRFSLVKSILIPAAFPSILTGLKVGWAFAWRTLIAAELVFGVSSGAGGLGWYIFE 245 Query: 245 NRNELYTDKVFAGLAMVILIGLVVENFGFNTIEKLTVKRWGMQR 288 NRN+L T VF+GL VI+IGLVVEN F IEK T++RWGMQ Sbjct: 246 NRNQLETANVFSGLFFVIIIGLVVENLIFAQIEKRTIRRWGMQH 289 >gi|300309716|ref|YP_003773808.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 273 Score = 97.8 bits (242), Expect = 9e-22, Method: Compositional matrix adjust. Identities = 70/218 (32%), Positives = 115/218 (52%), Gaps = 4/218 (1%) Query: 52 WEIIARIQDNDLLLPSFAQTAHAFADGVITGELIGKVWISLKVLVKGYLIGIALAFALTT 111 W ++ + + L + A ++ +GEL+ SL +V G+LIG LA L Sbjct: 41 WAVLRKWVEYATPLKPYDPEAGSWLAWAFSGELVMDAIGSLYRVVAGFLIGAGLALPLGL 100 Query: 112 LAVSTQLGRDLLSTLTSMFNPLPAIAMLPLALLWFGLGENSLIFVLVHSVLWALALNTYA 171 L S+Q L++ + P+P IA +PLA+LWFGLG +F++ + + +NT A Sbjct: 101 LMGSSQRIYGLMNLTVQVIRPIPPIAYIPLAILWFGLGNPPALFLISLGAFFPVLMNTIA 160 Query: 172 GFLGVSDTLRMAGRNYGLRGLRFVLFMLVPAALPSILAGLRIGWAFAWRTLIAAELVFGA 231 G V A RN G L +++PAA+P IL+G+RIG A+ +I AE++ Sbjct: 161 GVRQVDSIYLRAARNLGASQSTLFLRVMLPAAVPYILSGVRIGIGTAFIVVIVAEMI--- 217 Query: 232 SSGKGGLGWYIFQNRNELYTDKVFAGLAMVILIGLVVE 269 + GLG+ I + R ++DK+ AG+ + L+GL ++ Sbjct: 218 -AVSNGLGFRIMEAREYFWSDKIIAGMITIGLLGLAID 254 >gi|300310979|ref|YP_003775071.1| alkanesulfonates ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 279 Score = 85.5 bits (210), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 51/149 (34%), Positives = 79/149 (53%), Gaps = 4/149 (2%) Query: 122 LLSTLTSMFNPLPAIAMLPLALLWFGLGENSLIFVLVHSVLWALALNTYAGFLGVSDTLR 181 LL T M +PA+A++PL +LWFG+ E + +F++ V + + LNT+ G V L Sbjct: 118 LLDTTLQMVRNIPALALIPLVILWFGIDETAKLFLVSVGVFFPVYLNTFHGIRSVDQGLI 177 Query: 182 MAGRNYGLRGLRFVLFMLVPAALPSILAGLRIGWAFAWRTLIAAELVFGASSGKGGLGWY 241 ++YGL G +++P ALP+IL GLR W LI AE + S + G+G+ Sbjct: 178 EMAKSYGLSGWPLYRDVILPGALPAILVGLRFALGLVWVLLIVAETI----SAQAGIGYM 233 Query: 242 IFQNRNELYTDKVFAGLAMVILIGLVVEN 270 R L TD V G+ + L+G + + Sbjct: 234 TMNAREFLQTDVVLVGILLYALLGKLADT 262 >gi|300309985|ref|YP_003774077.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 278 Score = 83.6 bits (205), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 59/189 (31%), Positives = 91/189 (48%), Gaps = 4/189 (2%) Query: 64 LLPSFAQTAHAFADGVITGELIGKVWISLKVLVKGYLIGIALAFALTTLAVSTQLGRDLL 123 LLP ++ AH GEL+ + SL + G++IG ALA AL L ++ LL Sbjct: 52 LLPPPSELAHTLWSLAQRGELLPHIAASLARVAAGFVIGSALAVALGLLVGISRRAEALL 111 Query: 124 STLTSMFNPLPAIAMLPLALLWFGLGENSLIFVLVHSVLWALALNTYAGFLGVSDTLRMA 183 +P++A +PL LLW G+ E + ++ + + LN AG + L Sbjct: 112 EPSFQALRAIPSLAWIPLLLLWLGIDETPKVVLIAIGAFFPVYLNLVAGIRDIDHKLIEV 171 Query: 184 GRNYGLRGLRFVLFMLVPAALPSILAGLRIGWAFAWRTLIAAELVFGASSGKGGLGWYIF 243 G GL + +L+PA+LP + AGLR G + AW L+AAEL+ + G+G+ + Sbjct: 172 GTTSGLNTQALIGHILLPASLPYLFAGLRGGLSLAWMFLVAAELI----AATKGVGYLLS 227 Query: 244 QNRNELYTD 252 R D Sbjct: 228 DGRESSRPD 236 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.142 0.436 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 901,976 Number of extensions: 37749 Number of successful extensions: 208 Number of sequences better than 1.0e-08: 15 Number of HSP's gapped: 199 Number of HSP's successfully gapped: 15 Length of query: 288 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 204 Effective length of database: 1,222,130 Effective search space: 249314520 Effective search space used: 249314520 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 130 (54.7 bits)