BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0357.p (323 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310572|ref|YP_003774664.1| drug/metabolite transporter (DM... 135 6e-33 >gi|300310572|ref|YP_003774664.1| drug/metabolite transporter (DMT) superfamily permease [Herbaspirillum seropedicae SmR1] Length = 312 Score = 135 bits (339), Expect = 6e-33, Method: Compositional matrix adjust. Identities = 107/315 (33%), Positives = 159/315 (50%), Gaps = 36/315 (11%) Query: 4 GILCALCAGLMWGLVFVTPLLLGDYPGVVLSLGRYVAFGLIA---LAPAWFDRRRIGLLS 60 GIL + AG WGLVF+ P L + L+ GRY+A+GL A +AP RR + L Sbjct: 9 GILYSALAGAAWGLVFLAPELTRSFSPWQLTAGRYLAYGLFAGVLIAPR--LRRLLPHLG 66 Query: 61 RQDWTVAFKLALVGNLIYYAMLATAIQLADAPLPTMLIGTLPIVISVCANWRPGHVSESV 120 R +W L+L+GN++YY LA+A+QL + +++IG LP+ +S+ + G +V Sbjct: 67 RDEWRALVWLSLLGNVVYYVFLASAVQLGGMAMTSLVIGFLPVAVSIIGSRGEG----AV 122 Query: 121 AWSRLAPSLMILGLGLTLVNSSELERLRDTIGNARSTTDYALGCLIAVCAIAAWTWYPIV 180 +L PSL LGL + + L ++ A D +G A+ A+ +WT Y + Sbjct: 123 PLRKLVPSL---ALGLAGIACVAWQSL--SVSAAGQARDSVIGFFCALAALISWTTYAVG 177 Query: 181 NSRYLRTHTHINSVTW----ATAQGLATLPMALIGFALFGIYGVASGSDYAFPLGPRPGT 236 NSR+L HI++ W GL +L +A+ F + L P PG Sbjct: 178 NSRWLARLHHISAHDWNLLIGVVTGLLSLFVAVPAFTVM--------------LQPHPGH 223 Query: 237 ----FVVMMLVIGLSASWLGTLLWNKASQLLPTALVAQLIVFETLAALLYAFLLRGVAPD 292 F + + + AS G WNKAS+LLP ++ Q+I+FETL ALLY FL P Sbjct: 224 DWLHFAAVSAGVAICASLFGNACWNKASRLLPLTMIGQMILFETLFALLYGFLWEQRWPT 283 Query: 293 LRVLAGAVLLCIGVV 307 L +A VL+ V+ Sbjct: 284 LLEIAAMVLVTASVL 298 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.141 0.451 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,093,482 Number of extensions: 47150 Number of successful extensions: 231 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 228 Number of HSP's successfully gapped: 1 Length of query: 323 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 238 Effective length of database: 1,217,395 Effective search space: 289740010 Effective search space used: 289740010 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 131 (55.1 bits)