BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0347.p (509 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309431|ref|YP_003773523.1| ammonium transporter transmembr... 850 0.0 gi|300309914|ref|YP_003774006.1| ammonium transporter transmembr... 116 5e-27 >gi|300309431|ref|YP_003773523.1| ammonium transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 510 Score = 850 bits (2196), Expect = 0.0, Method: Compositional matrix adjust. Identities = 462/508 (90%), Positives = 473/508 (93%), Gaps = 3/508 (0%) Query: 1 MFSSALAIGTLLLAVSFAAPVQAQDAPKPEAAAAAAPAAEAPKADAPKAEAPAAAPAAAA 60 MFS ALAIG LL AVSF AP QAQDAPKPEAAAAAA A A A A AA A AA Sbjct: 6 MFSGALAIGALLFAVSFTAPAQAQDAPKPEAAAAAAAAPAAAPAAPKAEAAAPAAAAPAA 65 Query: 61 AAAPAAAAAAPVLVPNKGDTAWMLVCTAFVILMTLPGLGLFYGGLVRSKNMLSVLMQCFV 120 AA AAA A VPNKGDTAWMLVCTAFVILMTLPGLGLFYGGLVRSKNMLSVLMQCF+ Sbjct: 66 PAAAAAAPAP---VPNKGDTAWMLVCTAFVILMTLPGLGLFYGGLVRSKNMLSVLMQCFM 122 Query: 121 IFSLIYILWFVYGYSIAFTEGNAFFGGFDRLFLHGLNGDSTTATFSKGVVIPELSFVAFQ 180 IFSLIYILWFVYGYSIAFTEGNAFFGGFDRLFLHGL GDS T TFSKGVVIPEL FVAFQ Sbjct: 123 IFSLIYILWFVYGYSIAFTEGNAFFGGFDRLFLHGLTGDSNTGTFSKGVVIPELVFVAFQ 182 Query: 181 GAFAAITVALIIGSFAERVKFSAVIAFSILWFTFSYLPMAHMVWFWPGPDAFTDAAAAEK 240 GAFAAITVALIIG+FAERVKFSAVI FSILWFTFSYLPMAHMVWFWPGPDAFTDAAA EK Sbjct: 183 GAFAAITVALIIGAFAERVKFSAVILFSILWFTFSYLPMAHMVWFWPGPDAFTDAAAGEK 242 Query: 241 ATAISGWLFQKGALDYAGGTVVHINAAIAGLVGSYVIGKRVGYGKEAFKPHSLTLTMVGA 300 ATAISGWLFQKGALDYAGGTVVHINAAIAGLVGSYVIGKR+G+GKEAFKPHSLTLTMVGA Sbjct: 243 ATAISGWLFQKGALDYAGGTVVHINAAIAGLVGSYVIGKRIGFGKEAFKPHSLTLTMVGA 302 Query: 301 SLLWFGWFGFNAGSGLEANGGAALSFVNTILATAAAVLSWSFGEWIGKGKPSMLGAASGA 360 SLLWFGWFGFNAGSGLEANG AAL+FVNTILATAAAVLSWSFGEWIGKGKPSMLGAASGA Sbjct: 303 SLLWFGWFGFNAGSGLEANGTAALAFVNTILATAAAVLSWSFGEWIGKGKPSMLGAASGA 362 Query: 361 VAGLVAITPAAGFVGVCGAVVMGLLAGLLCLWGVTGLKKLLGSDDALDVFGVHGVGGILG 420 VAGLVAITPAAGFVGV GAVVMGL+AGLLCLWGV+GLKK+LGSDDALDVFGVHGVGGILG Sbjct: 363 VAGLVAITPAAGFVGVIGAVVMGLIAGLLCLWGVSGLKKILGSDDALDVFGVHGVGGILG 422 Query: 421 AILTGVFAAPSLGGTGIYDYVANAVAPDYSIGGQVWIQFQGVLTTVILSGVVSFIAYKLV 480 AILTGVFAAPSLGGTGIYDYVANAVAPDYSIGGQVWIQFQGVLTTV+LSGVVSFIAYK+V Sbjct: 423 AILTGVFAAPSLGGTGIYDYVANAVAPDYSIGGQVWIQFQGVLTTVVLSGVVSFIAYKIV 482 Query: 481 DIVIGLRVTEEEEREGLDISSHGESAYH 508 D+VIGLRVTEEEEREGLDISSHGESAYH Sbjct: 483 DVVIGLRVTEEEEREGLDISSHGESAYH 510 >gi|300309914|ref|YP_003774006.1| ammonium transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 401 Score = 116 bits (290), Expect = 5e-27, Method: Compositional matrix adjust. Identities = 118/439 (26%), Positives = 192/439 (43%), Gaps = 59/439 (13%) Query: 76 NKGDTAWMLVCTAFVILMTLPGLGLFYGGLVRSKNMLSVLMQCFVIFSLIYILWFVYGYS 135 G A ++ A +IL G G VR KN ++ L++ F++ I++F GY Sbjct: 6 KNGADALFILLGAIMILAMHAGFAFLELGTVRKKNQVNALVKILADFAVSTIVYFFIGYY 65 Query: 136 IAFTEGNAFFGGFDRLFLHGLNGDSTTATFSKGVVIPELSFVAFQGAFAAITVALIIGSF 195 +A+ +FF G + L + + F EL F FAA A+I G Sbjct: 66 VAYRV--SFFAGAETL--------AQRSGF-------ELVKFFFLLTFAAAIPAIISGGI 108 Query: 196 AERVKFSAVIAFSILWFTFSYLPMAHMVWFWPGPDAFTDAAAAEKATAISGWL---FQKG 252 AER KF+ + + + Y F + A + + WL F Sbjct: 109 AERSKFNPQLVATAVLVGLVY--------------PFFEGIAWNQHLGVQAWLAATFDAE 154 Query: 253 ALDYAGGTVVHINAAIAGLVGSYVIGKRVG-YGKE----AFKPHSLTLTMVGASLLWFGW 307 D+AG VVH L ++G R G Y KE A P ++ +GA +L GW Sbjct: 155 FHDFAGSIVVHAVGGWIALPAVLLLGARRGRYSKEGAVAAHPPSNIPFLALGAWILTVGW 214 Query: 308 FGFNAGSGLEANGGAALSFVNTILATAAAVLSWSFGEWIGKGKPSMLGAASGAVAGLVAI 367 FGFN S + L +N+++A A L +G+ P A +G +AGLVA+ Sbjct: 215 FGFNVMSAQTLDKMNGLVAMNSLMAMAGGTL---VALLMGRNDPGF--AYNGPLAGLVAV 269 Query: 368 TPAAGFVGVCGAVVMGLLAGLLCLWGVTGLKKLLGSDDALDVFGVHGVGGILGAILTGVF 427 + + GA+V G +AG + +W T + DD L V+ +HG+ G+ G + G+F Sbjct: 270 CAGSDLMHPLGALVTGGIAGAIFVWMFTRTQNKWKIDDVLGVWPLHGLCGLWGGLAAGIF 329 Query: 428 AAPSLGGTGIYDYVANAVAPDYSIGGQVWIQFQGVLTTVILSGVVSFIAYKLVDIVIGLR 487 +LGG G +++ Q G + ++++ + +I Y L+ +G+R Sbjct: 330 GLQALGGRGGVSFLS---------------QLIGSVMGIVIAALGGWIVYGLLKKAVGIR 374 Query: 488 VTEEEEREGLDISSHGESA 506 + E+E EG D++ H S+ Sbjct: 375 LDPEQEFEGADLAIHKISS 393 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.137 0.420 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,394,894 Number of extensions: 57322 Number of successful extensions: 252 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 245 Number of HSP's successfully gapped: 2 Length of query: 509 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 420 Effective length of database: 1,198,455 Effective search space: 503351100 Effective search space used: 503351100 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 133 (55.8 bits)