BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0034.0346.p
         (431 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300309432|ref|YP_003773524.1| glutamate-cysteine ligase [Herb...   799   0.0  

>gi|300309432|ref|YP_003773524.1| glutamate-cysteine ligase
           [Herbaspirillum seropedicae SmR1]
          Length = 433

 Score =  799 bits (2063), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 380/433 (87%), Positives = 411/433 (94%), Gaps = 2/433 (0%)

Query: 1   MVPHLVTALNGPLLDLEKKILGATPAIERWFRMEWQEHTPPFYCSVDLRNAGFKLAPVDT 60
           MVPHLVTALNGPLL+LEKKILGATPAIERWFRMEWQEHTPPFYCSVDLRNAGFKLAPVDT
Sbjct: 1   MVPHLVTALNGPLLELEKKILGATPAIERWFRMEWQEHTPPFYCSVDLRNAGFKLAPVDT 60

Query: 61  NLFPGGFNNLSPEMLPLAVQAAMAAIEKYCPDAKNLLLIPENHIRNSFYLQNVARLMQIF 120
           NLFPGGFNNLSPEMLPLAVQAAMAAIEKYCPDA+NLLLIPENH  N+FYLQN+ARLMQIF
Sbjct: 61  NLFPGGFNNLSPEMLPLAVQAAMAAIEKYCPDARNLLLIPENHTGNTFYLQNIARLMQIF 120

Query: 121 RQTGLNVRLGTLE-DIKKPKLIDLPDGSTITLEPLERSKNGRRLGLKNFDPCTVLLNNDL 179
           RQTGLNVRLG+L   + KPK I+LPDG+TITLEPLERS NGRRLGLKNFDPCTVLLNNDL
Sbjct: 121 RQTGLNVRLGSLAPGVNKPKPIELPDGTTITLEPLERSANGRRLGLKNFDPCTVLLNNDL 180

Query: 180 SIGIPSVLENLDEQNVLPPLHAGWAVRRKSNHFSAYDDVVKKFAKLVDVDPWMLNPLYAK 239
           SIGIPSVLE L EQ+VLPPLHAGWA+RRKSNHF+AYD+VVKKFAK+V+VDPWMLNPL+AK
Sbjct: 181 SIGIPSVLEGLHEQSVLPPLHAGWAMRRKSNHFAAYDEVVKKFAKMVEVDPWMLNPLFAK 240

Query: 240 CGELNLQEDAGSDCLADNVSSLLSKIRKKYKEYGIKEQPFVIVKANAGTYGMGIMTVKDA 299
             +++ QE  G+D LAD+VSSLLSKIRKKYKEYGIKEQPFV VKANAGTYGMGIMTVKDA
Sbjct: 241 VSDIDFQESTGTDALADSVSSLLSKIRKKYKEYGIKEQPFVFVKANAGTYGMGIMTVKDA 300

Query: 300 SEVKDLNRQQRSK-LAVGKEGLEVNELVIQEGVHSFERINEAVAEPVVYMIDRYVVGGFY 358
           SE+KDLNR+QR+  LA GKEGLEV+E+++QEGVHSFERIN+AVAEPVVYMIDRYVVGGFY
Sbjct: 301 SEIKDLNRKQRTGMLAAGKEGLEVSEVIVQEGVHSFERINQAVAEPVVYMIDRYVVGGFY 360

Query: 359 RVHADRDINENLNAPGAHFVPLAFQQQHALPDLQAKPGTAAPNRFYMYGVVARLALLAAS 418
           RVHADRD+NENLNAPGAHFVPLAF QQHALPD+ AKPGTAAPNRFYMYGVVARLALLAAS
Sbjct: 361 RVHADRDVNENLNAPGAHFVPLAFAQQHALPDMHAKPGTAAPNRFYMYGVVARLALLAAS 420

Query: 419 LEMEKTDPNPEVY 431
           +EMEKTDPNPEVY
Sbjct: 421 VEMEKTDPNPEVY 433


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.319    0.137    0.407 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,339,947
Number of extensions: 55328
Number of successful extensions: 132
Number of sequences better than 1.0e-08: 1
Number of HSP's gapped: 130
Number of HSP's successfully gapped: 1
Length of query: 431
Length of database: 1,619,870
Length adjustment: 88
Effective length of query: 343
Effective length of database: 1,203,190
Effective search space: 412694170
Effective search space used: 412694170
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 132 (55.5 bits)