BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0323.p (689 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309453|ref|YP_003773545.1| elongation factor G1 [Herbaspir... 1280 0.0 gi|300313617|ref|YP_003777709.1| translation elongation factor [... 1038 0.0 gi|300312224|ref|YP_003776316.1| peptide chain release factor 3 ... 153 5e-38 gi|300311258|ref|YP_003775350.1| GTP-binding elongation factor p... 89 2e-18 gi|300312895|ref|YP_003776987.1| GTP-binding elongation factor p... 85 2e-17 >gi|300309453|ref|YP_003773545.1| elongation factor G1 [Herbaspirillum seropedicae SmR1] Length = 701 Score = 1280 bits (3311), Expect = 0.0, Method: Compositional matrix adjust. Identities = 612/645 (94%), Positives = 630/645 (97%) Query: 1 MARKTPIERYRNIGISAHIDAGKTTTTERVLFYTGVNHKIGEVHDGAATMDWMEQEQERG 60 MARKTPIERYRNIGISAHIDAGKTTTTERVLFYTGVNHKIGEVHDGAATMDWMEQEQERG Sbjct: 1 MARKTPIERYRNIGISAHIDAGKTTTTERVLFYTGVNHKIGEVHDGAATMDWMEQEQERG 60 Query: 61 ITITSAATTCFWKGMANNFPAHHINIIDTPGHVDFTIEVERSMRVLDGACMVYCAVGGVQ 120 ITITSAATTC+WKGMANNFPAHHINIIDTPGHVDFTIEVERSMRVLDGACMVYCAVGGVQ Sbjct: 61 ITITSAATTCYWKGMANNFPAHHINIIDTPGHVDFTIEVERSMRVLDGACMVYCAVGGVQ 120 Query: 121 PQSETVWRQANKYKVPRLAFVNKMDRTGANFFKVYDQMRARLKANPIPMQVPIGAEDNFE 180 PQSETVWRQANKYKVPRLAFVNKMDRTGANFFKVY+QMRARLKANPIPMQVPIGAE+NFE Sbjct: 121 PQSETVWRQANKYKVPRLAFVNKMDRTGANFFKVYEQMRARLKANPIPMQVPIGAEENFE 180 Query: 181 GVIDLVKMRAIYWDDASQGMKFDYRDIPEHLKAEAQKWRENMVEAAAEASEELMNKYLED 240 GVIDLVKMRAIYWDDASQGMKFDYRDIPEHLKA+AQKWREN+VE AAEASE+LMNKYLE+ Sbjct: 181 GVIDLVKMRAIYWDDASQGMKFDYRDIPEHLKADAQKWRENLVETAAEASEDLMNKYLEE 240 Query: 241 GDLSEEEIKAAIRQRTIASEIVPMMCGTAFKNKGVQAMLDGVIEYLPSPVDIPPVPGLDE 300 GDL+E EIK AIRQRTI+ EIVPMMCGTAFKNKGVQAMLDGV+EYLPSPVDIPPVPGL+E Sbjct: 241 GDLTEAEIKGAIRQRTISGEIVPMMCGTAFKNKGVQAMLDGVVEYLPSPVDIPPVPGLNE 300 Query: 301 DDEPVVRKAEDTEKFSALAFKIATDPFVGQLCFIRCYSGTLNSGDTVFNSVKSKKERIGR 360 DDEPVVR+A+D EKFSALAFKIATDPFVGQLCFIRCYSGTLNSGDTV NSVKSKKERIGR Sbjct: 301 DDEPVVREAKDDEKFSALAFKIATDPFVGQLCFIRCYSGTLNSGDTVLNSVKSKKERIGR 360 Query: 361 IVQMHANQREEIKEMLAGDIAAVVGLKDTTTGDTLCDDKAVVVLERMVFPEPVISQAVEP 420 IVQMHANQREEIKEM+AGDIAAVVGLKDTTTGDTLCDDKA+VVLERMVFPEPVISQAVEP Sbjct: 361 IVQMHANQREEIKEMMAGDIAAVVGLKDTTTGDTLCDDKAMVVLERMVFPEPVISQAVEP 420 Query: 421 KTKADQEKMGLALNRLAAEDPSFRVRTDEESGQTIIAGMGELHLDIIVDRMKREFNVEAT 480 KTKADQEKMGLALNRLAAEDPSFRVRTDEESGQTIIAGMGELHLDIIVDRMKREFNVEAT Sbjct: 421 KTKADQEKMGLALNRLAAEDPSFRVRTDEESGQTIIAGMGELHLDIIVDRMKREFNVEAT 480 Query: 481 VGKPQVAYRETIRKTCEEIEGKFVKQSGGRGQYGHVVLKIEPQEPGKGFEFVDAIKGGTV 540 VGKPQVAYRETIRKTCEE EGKFVKQSGGRGQYGHVVLKIEPQE GKGFEFVDAIKGGTV Sbjct: 481 VGKPQVAYRETIRKTCEESEGKFVKQSGGRGQYGHVVLKIEPQEAGKGFEFVDAIKGGTV 540 Query: 541 PREFIPAVEKGVRETLNSGVLAGYPVVDVKVTLFFGSYHDVDSNENAFRMAGSMAFKDGC 600 PREFIPAVEKGVRETLN+GVLAGYPVVDVKVTLFFGSYHDVDSNENAFRMAGSMAFK+GC Sbjct: 541 PREFIPAVEKGVRETLNTGVLAGYPVVDVKVTLFFGSYHDVDSNENAFRMAGSMAFKEGC 600 Query: 601 RKASPVILEPMMAVEVETPEDYAGTVMGDLSSRRGDHQGRSAAVG 645 R+ASPVILEPMMAVEVETPEDYAGTVMGDLSSRRG QG G Sbjct: 601 RRASPVILEPMMAVEVETPEDYAGTVMGDLSSRRGMVQGMDEIAG 645 >gi|300313617|ref|YP_003777709.1| translation elongation factor [Herbaspirillum seropedicae SmR1] Length = 701 Score = 1038 bits (2685), Expect = 0.0, Method: Compositional matrix adjust. Identities = 492/650 (75%), Positives = 561/650 (86%) Query: 1 MARKTPIERYRNIGISAHIDAGKTTTTERVLFYTGVNHKIGEVHDGAATMDWMEQEQERG 60 M+RKT IE YRNIGISAHIDAGKTTTTER+LFYTGVNHKIGEVH+GAATMDWMEQEQERG Sbjct: 1 MSRKTRIENYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVHNGAATMDWMEQEQERG 60 Query: 61 ITITSAATTCFWKGMANNFPAHHINIIDTPGHVDFTIEVERSMRVLDGACMVYCAVGGVQ 120 ITITSAATT FWKGMA N+P H INIIDTPGHVDFTIEVERSMRVLDGA MVY +VGGVQ Sbjct: 61 ITITSAATTAFWKGMAGNYPEHRINIIDTPGHVDFTIEVERSMRVLDGAVMVYDSVGGVQ 120 Query: 121 PQSETVWRQANKYKVPRLAFVNKMDRTGANFFKVYDQMRARLKANPIPMQVPIGAEDNFE 180 PQSETVWRQANKYKVPR+AFVNKMDR GA+FF+V Q+ RLK +P+Q+P+GAED+F Sbjct: 121 PQSETVWRQANKYKVPRIAFVNKMDRIGADFFRVQKQIEERLKGKAVPIQIPVGAEDHFS 180 Query: 181 GVIDLVKMRAIYWDDASQGMKFDYRDIPEHLKAEAQKWRENMVEAAAEASEELMNKYLED 240 GVIDLVKM+AI WD+ASQG+ F Y DIP L+ A+ WR+ MVE AAEA+EEL+ KYL Sbjct: 181 GVIDLVKMKAIIWDEASQGVLFKYEDIPLELEDTARHWRDLMVEQAAEANEELLEKYLSG 240 Query: 241 GDLSEEEIKAAIRQRTIASEIVPMMCGTAFKNKGVQAMLDGVIEYLPSPVDIPPVPGLDE 300 L+E++IK +R RT+A+EIVPM+ G+AFKNKGVQAMLD VI+YLPSPVD+P + G E Sbjct: 241 NPLTEDDIKRGLRLRTVANEIVPMLAGSAFKNKGVQAMLDAVIDYLPSPVDVPAIAGHGE 300 Query: 301 DDEPVVRKAEDTEKFSALAFKIATDPFVGQLCFIRCYSGTLNSGDTVFNSVKSKKERIGR 360 DD + R D E FSALAFKI TDPFVGQL F R YSG +NSGDTV+N VK KKER+GR Sbjct: 301 DDSEIERHPSDEEPFSALAFKIMTDPFVGQLTFFRVYSGIVNSGDTVYNPVKGKKERLGR 360 Query: 361 IVQMHANQREEIKEMLAGDIAAVVGLKDTTTGDTLCDDKAVVVLERMVFPEPVISQAVEP 420 I+QMHAN+R+EIKE+ AGDIAA VGLKD TTGDTL D + ++LERM+FPEPVISQAVEP Sbjct: 361 ILQMHANERKEIKEVFAGDIAAAVGLKDVTTGDTLSDPEHPIILERMIFPEPVISQAVEP 420 Query: 421 KTKADQEKMGLALNRLAAEDPSFRVRTDEESGQTIIAGMGELHLDIIVDRMKREFNVEAT 480 KTKADQEKMG+ALNRLA EDPSFRV TDEESGQTI++GMGELHL+I+VDRMKREFNVEAT Sbjct: 421 KTKADQEKMGIALNRLAQEDPSFRVHTDEESGQTIMSGMGELHLEILVDRMKREFNVEAT 480 Query: 481 VGKPQVAYRETIRKTCEEIEGKFVKQSGGRGQYGHVVLKIEPQEPGKGFEFVDAIKGGTV 540 VGKPQVAYRE IRK E++EGKFVKQSGGRGQYGHVV+K+EPQ GKG+EFVDAIKGG V Sbjct: 481 VGKPQVAYREAIRKAVEDVEGKFVKQSGGRGQYGHVVIKLEPQPAGKGYEFVDAIKGGVV 540 Query: 541 PREFIPAVEKGVRETLNSGVLAGYPVVDVKVTLFFGSYHDVDSNENAFRMAGSMAFKDGC 600 PREFIPAV+KG++E+L +GVLAGYPVVDVK TL FGSYHDVDSNENAFRMAGSMAFK+ Sbjct: 541 PREFIPAVDKGIQESLKAGVLAGYPVVDVKATLTFGSYHDVDSNENAFRMAGSMAFKEAM 600 Query: 601 RKASPVILEPMMAVEVETPEDYAGTVMGDLSSRRGDHQGRSAAVGNVRLL 650 ++A P++LEPMM VEVETPE++ G VMGDLSSRRG QG VG +L+ Sbjct: 601 KRAGPMLLEPMMQVEVETPEEFMGNVMGDLSSRRGMVQGMEDMVGGGKLV 650 >gi|300312224|ref|YP_003776316.1| peptide chain release factor 3 [Herbaspirillum seropedicae SmR1] Length = 530 Score = 153 bits (386), Expect = 5e-38, Method: Compositional matrix adjust. Identities = 131/519 (25%), Positives = 230/519 (44%), Gaps = 54/519 (10%) Query: 7 IERYRNIGISAHIDAGKTTTTERVLFYTGVNHKIGEVHDGA----ATMDWMEQEQERGIT 62 ++R R GI +H DAGKTT TE++L ++G G V AT DWME E++RGI+ Sbjct: 6 VKRRRTFGIISHPDAGKTTLTEKLLLFSGAIQLAGTVKARKSGRHATSDWMEIEKQRGIS 65 Query: 63 ITSAATTCFWKGMANNFPAHHINIIDTPGHVDFTIEVERSMRVLDGACMVYCAVGGVQPQ 122 + S+ M + H +N++DTPGH DF+ + R + +D A MV A GV+ Q Sbjct: 66 VASSV-------MQFEYRDHVVNLLDTPGHQDFSEDTYRVLTAVDSALMVIDAAKGVEEQ 118 Query: 123 SETVWRQANKYKVPRLAFVNKMDRTGANFFKVYDQMRARLKANPIPMQVPIGAEDNFEGV 182 + + P + F+NK+DR + ++ D++ + LK P+ PIG NF GV Sbjct: 119 TIKLLNVCRMRNTPIITFMNKLDRETRDSLELLDELESVLKIQCAPVTWPIGMGKNFRGV 178 Query: 183 IDLVKMRAIYWDDASQGMK--------FDYRDIPEHLKAEAQKWRENMVEAAAEASEELM 234 L++ + + + D + E E ++ + VE AS Sbjct: 179 YHLLRDEIMLFKAGEERANGNVEIIKGIDNPKLAEMFPLEIEQLKME-VELVHGASHPF- 236 Query: 235 NKYLEDGDLSEEEIKAAIRQRTIASEIVPMMCGTAFKNKGVQAMLDGVIEYLPSPVDIPP 294 L+D +A P+ G+A N GV+ +LD ++++ P P Sbjct: 237 --NLDD---------------FLAGVQTPVFFGSAINNFGVREILDALLDWAPGP----- 274 Query: 295 VPGLDEDDEPVVRKAEDTEK-FSALAFKIAT--DP-FVGQLCFIRCYSGTLNSGDTVFNS 350 G D VR+ + TE+ FS FKI DP ++ F+R SG G + + Sbjct: 275 -GGRD----ATVREVKPTEEPFSGFVFKIQANMDPAHRDRIAFLRVCSGRFERGMKLKHL 329 Query: 351 VKSKKERIGRIVQMHANQREEIKEMLAGDIAAVVGLKDTTTGDTLCDDKAVVVLERMVFP 410 ++ ++ +V A+ RE+++E AGDI + + GD+ + + ++ + + Sbjct: 330 RLNRDIKVSSVVTFMASSREQVEEAYAGDIIGLPNHGNMQIGDSFSEGE-MLQFTGIPYF 388 Query: 411 EPVISQAVEPKTKADQEKMGLALNRLAAEDPSFRVRTDEESGQTIIAGMGELHLDIIVDR 470 P + + +++ L +L E+ + +V I+ +G L +++ R Sbjct: 389 APDFFRVARIRNPLKIKQLHKGLQQL-GEEGAVQVFKPVTGSDLILGAVGVLQFEVVASR 447 Query: 471 MKREFNVEATVGKPQVAYRETIRKTCEEIEGKFVKQSGG 509 + E+ V+A ++ I ++ F K S G Sbjct: 448 LLNEYGVDAVFEGTSISSARWISCDDKKKLADFEKSSAG 486 >gi|300311258|ref|YP_003775350.1| GTP-binding elongation factor protein [Herbaspirillum seropedicae SmR1] Length = 597 Score = 88.6 bits (218), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 58/179 (32%), Positives = 93/179 (51%), Gaps = 12/179 (6%) Query: 11 RNIGISAHIDAGKTTTTERVL-FYTGVNHKIGEVHDGAATMDWMEQEQERGITITSAATT 69 RN I AHID GK+T +R++ G++++ E A +D M+ E+ERGITI + Sbjct: 5 RNFSIIAHIDHGKSTLADRIIQLCGGLSNREME----AQVLDSMDIERERGITIKAQTAA 60 Query: 70 CFWKGMANNFPAHHINIIDTPGHVDFTIEVERSMRVLDGACMVYCAVGGVQPQSETVWRQ 129 +K A + +++N+IDTPGHVDF+ EV RS+ +GA +V A GV+ Q+ Sbjct: 61 LSYK--AKDGQIYNLNLIDTPGHVDFSYEVSRSLSACEGALLVVDASQGVEAQTVANCYT 118 Query: 130 ANKYKVPRLAFVNKMDRTGANFFKVYDQMRARL-----KANPIPMQVPIGAEDNFEGVI 183 A V + +NK+D A+ ++ + A P + +G ED E ++ Sbjct: 119 ALDLGVEVVPVLNKIDLPSADPDNAKSEIEDVIGIDASDATPCSAKTGLGVEDVLEAIV 177 >gi|300312895|ref|YP_003776987.1| GTP-binding elongation factor protein [Herbaspirillum seropedicae SmR1] Length = 627 Score = 85.1 bits (209), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 53/150 (35%), Positives = 80/150 (53%), Gaps = 16/150 (10%) Query: 18 HIDAGKTTTTERVLFYTGVNHKIGEVHDGAATMDWMEQEQERGITITSAATTCFWKGMAN 77 H+D GKTT +++L +G +V + MD + E+ERGITI S +KG Sbjct: 30 HVDHGKTTLVDQLLRQSGTFRDNQQVDN--RVMDSNDIEKERGITILSKNCAVEYKGT-- 85 Query: 78 NFPAHHINIIDTPGHVDFTIEVERSMRVLDGACMVYCAVGGVQPQSETVWRQANKYKVPR 137 HINI+DTPGH DF EVER + ++D ++ A G PQ+ V R+A + Sbjct: 86 -----HINIVDTPGHADFGGEVERVLSMVDSVLLLVDAQEGPMPQTRFVTRKALALGLKP 140 Query: 138 LAFVNKMDRTGAN-------FFKVYDQMRA 160 + +NK+DR GA F+++D++ A Sbjct: 141 IVVLNKIDRPGARAEWAINATFELFDKLGA 170 Score = 62.0 bits (149), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 59/229 (25%), Positives = 103/229 (44%), Gaps = 33/229 (14%) Query: 275 VQAMLDGVIEYLPSPVDIPPVPGLDEDDEPVVRKAEDTEKFSALAFKIATDPFVGQLCFI 334 ++ + D +++Y+P+ D P D P+ + E S +VG++ Sbjct: 200 MEPLFDAILKYVPARKDDP--------DAPLQLQITSLEYSS----------YVGKIGVG 241 Query: 335 RCYSGTLNSG-DTVFNSV---KSKKERIGRIVQMHANQREEIKEMLAGDIAAVVGLKDTT 390 R +G + G D V+ + K K RI +++ +R + E LAGDI + G+++ Sbjct: 242 RILAGRVKGGQDVVWMNGPDDKPTKARINQVLTFKGLERVLVDEALAGDIVLINGIEEIG 301 Query: 391 TGDTLCDDKAVVVLERMVFPEPVISQAVEPKTKADQEKMGLAL------NRLAAE---DP 441 G T+C A L + EP ++ T + G + +RL E + Sbjct: 302 IGSTICSPDAPNGLPMLKVDEPTLTMNFMVNTSPLAGREGKFVTTRQIRDRLEKELKSNM 361 Query: 442 SFRVRTDEESGQTI-IAGMGELHLDIIVDRMKREFNVEATVGKPQVAYR 489 + RV E T ++G GELHL I+++ M+RE E V +P+V +R Sbjct: 362 ALRVVQAENDDSTYEVSGRGELHLTILIENMRRE-GFELAVSRPRVVFR 409 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,129,753 Number of extensions: 90749 Number of successful extensions: 246 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 236 Number of HSP's successfully gapped: 9 Length of query: 689 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 597 Effective length of database: 1,184,250 Effective search space: 706997250 Effective search space used: 706997250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)