BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0321.p (398 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309454|ref|YP_003773546.1| GTPase translation elongation f... 778 0.0 gi|300309440|ref|YP_003773532.1| EF-Tu elongation factor protein... 778 0.0 gi|300312403|ref|YP_003776495.1| sulfate adenylyltransferase sub... 81 1e-16 gi|300312895|ref|YP_003776987.1| GTP-binding elongation factor p... 74 3e-14 gi|300312898|ref|YP_003776990.1| translation initiation factor I... 65 8e-12 >gi|300309454|ref|YP_003773546.1| GTPase translation elongation factor TU [Herbaspirillum seropedicae SmR1] Length = 396 Score = 778 bits (2010), Expect = 0.0, Method: Compositional matrix adjust. Identities = 379/395 (95%), Positives = 387/395 (97%) Query: 1 MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAIATVLSKKFGGEAKAYDQIDAAPEEKARG 60 MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAIATVLSKKFGGEAKAYDQIDAAPEEKARG Sbjct: 1 MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAIATVLSKKFGGEAKAYDQIDAAPEEKARG 60 Query: 61 ITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI 120 ITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI Sbjct: 61 ITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI 120 Query: 121 LLSRQVGVPYIIVFLNKADMVDDEELLELVEMEVRELLTKYEFPGDDLPIIKGSAKLALE 180 LLSRQVGVPYIIVFLNKADMVDD ELLELVEMEVRELL+KYEFPGDDLPI+KGSAKLALE Sbjct: 121 LLSRQVGVPYIIVFLNKADMVDDAELLELVEMEVRELLSKYEFPGDDLPIVKGSAKLALE 180 Query: 181 GDTGPLGEQAIMALAEALDTYIPTPERAVDGAFLLPVEDVFSISGRGTVVTGRVERGIVK 240 GDTGPLGEQAIMALAEALDTYIPTPERAVDG FL+PVEDVFSISGRGTVVTGRVERGI+K Sbjct: 181 GDTGPLGEQAIMALAEALDTYIPTPERAVDGTFLMPVEDVFSISGRGTVVTGRVERGIIK 240 Query: 241 VGEALQIVGIRDTQDTVCTGVEMFRKLLDQGQAGDNVGVLLRGTKREDVERGQVLAKPGS 300 VGE ++IVGI DTQ T CTGVEMFRKLLDQGQAGDNVGVLLRGTKREDV+RGQVLAKPGS Sbjct: 241 VGEEIEIVGIADTQKTTCTGVEMFRKLLDQGQAGDNVGVLLRGTKREDVQRGQVLAKPGS 300 Query: 301 IKPHKHFTGEIYVLSKDEGGRHTPFFNNYRPQFYFRTTDVTGSIELPKDKEMVMPGDNVS 360 IKPHKHFTGEIYVLSKDEGGRHTPFFNNYRPQFYFRTTDVTG+IELPKDKEMVMPGDNVS Sbjct: 301 IKPHKHFTGEIYVLSKDEGGRHTPFFNNYRPQFYFRTTDVTGAIELPKDKEMVMPGDNVS 360 Query: 361 ITVMLINPIAMEEGLRFAIREGGRTVGAGVVAKIL 395 ITV LINPIAMEEGLRFAIREGGRTVGAGVVAKI Sbjct: 361 ITVQLINPIAMEEGLRFAIREGGRTVGAGVVAKIF 395 >gi|300309440|ref|YP_003773532.1| EF-Tu elongation factor protein [Herbaspirillum seropedicae SmR1] Length = 396 Score = 778 bits (2010), Expect = 0.0, Method: Compositional matrix adjust. Identities = 379/395 (95%), Positives = 387/395 (97%) Query: 1 MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAIATVLSKKFGGEAKAYDQIDAAPEEKARG 60 MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAIATVLSKKFGGEAKAYDQIDAAPEEKARG Sbjct: 1 MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAIATVLSKKFGGEAKAYDQIDAAPEEKARG 60 Query: 61 ITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI 120 ITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI Sbjct: 61 ITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI 120 Query: 121 LLSRQVGVPYIIVFLNKADMVDDEELLELVEMEVRELLTKYEFPGDDLPIIKGSAKLALE 180 LLSRQVGVPYIIVFLNKADMVDD ELLELVEMEVRELL+KYEFPGDDLPI+KGSAKLALE Sbjct: 121 LLSRQVGVPYIIVFLNKADMVDDAELLELVEMEVRELLSKYEFPGDDLPIVKGSAKLALE 180 Query: 181 GDTGPLGEQAIMALAEALDTYIPTPERAVDGAFLLPVEDVFSISGRGTVVTGRVERGIVK 240 GDTGPLGEQAIMALAEALDTYIPTPERAVDG FL+PVEDVFSISGRGTVVTGRVERGI+K Sbjct: 181 GDTGPLGEQAIMALAEALDTYIPTPERAVDGTFLMPVEDVFSISGRGTVVTGRVERGIIK 240 Query: 241 VGEALQIVGIRDTQDTVCTGVEMFRKLLDQGQAGDNVGVLLRGTKREDVERGQVLAKPGS 300 VGE ++IVGI DTQ T CTGVEMFRKLLDQGQAGDNVGVLLRGTKREDV+RGQVLAKPGS Sbjct: 241 VGEEIEIVGIADTQKTTCTGVEMFRKLLDQGQAGDNVGVLLRGTKREDVQRGQVLAKPGS 300 Query: 301 IKPHKHFTGEIYVLSKDEGGRHTPFFNNYRPQFYFRTTDVTGSIELPKDKEMVMPGDNVS 360 IKPHKHFTGEIYVLSKDEGGRHTPFFNNYRPQFYFRTTDVTG+IELPKDKEMVMPGDNVS Sbjct: 301 IKPHKHFTGEIYVLSKDEGGRHTPFFNNYRPQFYFRTTDVTGAIELPKDKEMVMPGDNVS 360 Query: 361 ITVMLINPIAMEEGLRFAIREGGRTVGAGVVAKIL 395 ITV LINPIAMEEGLRFAIREGGRTVGAGVVAKI Sbjct: 361 ITVQLINPIAMEEGLRFAIREGGRTVGAGVVAKIF 395 >gi|300312403|ref|YP_003776495.1| sulfate adenylyltransferase subunit 1 protein [Herbaspirillum seropedicae SmR1] Length = 443 Score = 81.3 bits (199), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 112/439 (25%), Positives = 186/439 (42%), Gaps = 89/439 (20%) Query: 17 TIGHVDHGKTTLTAAIATVLSKKFGGEAKAYDQI-------------------DAAPEEK 57 T G VD GK+TL I +L G A D I D E+ Sbjct: 27 TAGSVDDGKSTL---IGRLLFDSKGIFADQLDAISRAKHKRTVGDTVDLSLLTDGLEAER 83 Query: 58 ARGITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAA------DG 111 +GITI+ A+ + T R + D PGH Y +NM+TGA+ D I++ + DG Sbjct: 84 EQGITIDVAYRYFATPKRKFIIADTPGHEQYTRNMVTGASTADAVIILVDVSKVKLGEDG 143 Query: 112 P---MPQTREHILLSRQVGVPYIIVFLNKADMVD-DEELLELVEMEVRELLTKY------ 161 + QT+ H ++ + + ++IV +NK D+VD D+ + + + ++ + Sbjct: 144 SVELLTQTKRHSTIAHLLQIQHVIVAVNKMDLVDYDQTVYDRIVGAYQQFAQQLGLQDVH 203 Query: 162 -----EFPGDDLPIIKGSAKLALEGDTGPLGEQAIMALAEALDTYIPTPERAVDGAFLLP 216 GD+ +++ SA++ GP ++ L E+L Y E A + AF P Sbjct: 204 AIPLSALAGDN--VVEASARMPWY--QGP----TLITLLESLSVY----EDAHEEAFRFP 251 Query: 217 VEDVFSISG------RGTVVTGRVERGIVKVGEALQIVGIRDTQDTVCTGVEMFRKLLDQ 270 V+ V +G RG + GR+E G V G+ L V + Q ++ L+ Sbjct: 252 VQLVARHNGHEANDFRGYM--GRIEAGKVARGDKL--VVLPSGQSATVKDIQTLDGSLES 307 Query: 271 GQAGDNVGVLLRGTKREDVERGQVL---AKPGSIKPHKHFTGEIYVLSKDE-GGRHTPFF 326 AG +V +LL + D+ RG +L A+P +I K ++ LS++ R + Sbjct: 308 ASAGQSVTILL--DEYLDISRGDLLASAAQPSTIL--KTVNADVCWLSEEPLDLRRKYWL 363 Query: 327 NNYRPQFYFRTTDVTGSIELPKDK----EMVMPGDNVSITVMLINPIAMEEGLRFAIREG 382 + Q R V +++ + E + D I+V + PIA + AIR Sbjct: 364 KHTTKQVAARVAKVDTLLDINTQERRPAETLKLNDIARISVNVQQPIAADA--YQAIRAT 421 Query: 383 G----------RTVGAGVV 391 G +TV AG++ Sbjct: 422 GAFILIDEVSHQTVAAGMI 440 >gi|300312895|ref|YP_003776987.1| GTP-binding elongation factor protein [Herbaspirillum seropedicae SmR1] Length = 627 Score = 73.6 bits (179), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 89/296 (30%), Positives = 130/296 (43%), Gaps = 33/296 (11%) Query: 20 HVDHGKTTLTAAIATVLSKKFGGEAKAYDQI-DAAPEEKARGITINTAHVEYETANRHYA 78 HVDHGKTTL + S F + +++ D+ EK RGITI + + E H Sbjct: 30 HVDHGKTTLVDQLLR-QSGTFRDNQQVDNRVMDSNDIEKERGITILSKNCAVEYKGTHIN 88 Query: 79 HVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLSRQVGVPYIIVFLNKA 138 VD PGHAD+ + + +D +L+ A +GPMPQTR + +G+ I+V LNK Sbjct: 89 IVDTPGHADFGGEVERVLSMVDSVLLLVDAQEGPMPQTRFVTRKALALGLKPIVV-LNKI 147 Query: 139 DMVDDEELLELVEMEVRELLTKYEFPGD--DLPII-----KGSAKL---ALEGDTGPLGE 188 D E EL K + D P+I G A L A EG+ P Sbjct: 148 DRPGARA--EWAINATFELFDKLGATEEQLDFPVIYASGLNGYASLDPEAREGNMEP--- 202 Query: 189 QAIMALAEALDTYIPTPERAVDGAFLLPVEDVFSISGRGTVVTGRVERGIVKVGEALQIV 248 L +A+ Y+P + D L + + S G + GR+ G VK G+ + + Sbjct: 203 -----LFDAILKYVPARKDDPDAPLQLQITSLEYSSYVGKIGVGRILAGRVKGGQDVVWM 257 Query: 249 GIRDTQDT------VCTGVEMFRKLLDQGQAGDNVGVLLRGTKREDVERGQVLAKP 298 D + T V T + R L+D+ AGD VL+ G E++ G + P Sbjct: 258 NGPDDKPTKARINQVLTFKGLERVLVDEALAGDI--VLINGI--EEIGIGSTICSP 309 >gi|300312898|ref|YP_003776990.1| translation initiation factor IF-2 [Herbaspirillum seropedicae SmR1] Length = 954 Score = 65.5 bits (158), Expect = 8e-12, Method: Compositional matrix adjust. Identities = 79/236 (33%), Positives = 103/236 (43%), Gaps = 37/236 (15%) Query: 15 VGTIGHVDHGKTTLTAAIATVLSKKFGGEAKAYDQIDAAPEEKARGITINTAHVEYETAN 74 V +GHVDHGKT+L I +K GEA GIT + ET Sbjct: 459 VTVMGHVDHGKTSLLDYIRR--TKVASGEAG--------------GITQHIGAYHVETPR 502 Query: 75 RHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLSRQVGVPYIIVF 134 +D PGH + GA D ILV +A DG MPQT+E I ++ GVP ++V Sbjct: 503 GMITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTKEAIAHAKAGGVP-LVVA 561 Query: 135 LNKAD---MVDDEELLELVEMEVRELLTKYEFPGDDLPIIKGSAKLALEGDTGPLGEQAI 191 +NK D D EL+ EV E+ G D P I SAK EG L + + Sbjct: 562 INKIDKPGANPDRVKQELIAEEV----VPEEY-GGDAPFIPVSAKTG-EGIDSLL--ENV 613 Query: 192 MALAEALDTYIP--TPERAVDGAFLLPVEDVFSISGRGTVVTGRVERGIVKVGEAL 245 + AE L+ P P R L +E GRG V T V+ G +K G+ + Sbjct: 614 LLQAEVLELKAPVEVPARG------LVIEAKLD-KGRGPVATILVQSGTLKRGDVV 662 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.137 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,233,603 Number of extensions: 51141 Number of successful extensions: 144 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 137 Number of HSP's successfully gapped: 9 Length of query: 398 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 311 Effective length of database: 1,207,925 Effective search space: 375664675 Effective search space used: 375664675 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)