BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0289.p (310 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313359|ref|YP_003777451.1| multidrug ABC transporter ATPas... 180 1e-46 gi|300311269|ref|YP_003775361.1| multidrug ABC transporter ATPas... 148 5e-37 gi|300311157|ref|YP_003775249.1| multidrug ABC transporter ATPas... 139 3e-34 gi|300310327|ref|YP_003774419.1| metal ion ABC transporter ATPas... 134 8e-33 gi|300313259|ref|YP_003777351.1| ABC transporter ATPase [Herbasp... 132 2e-32 >gi|300313359|ref|YP_003777451.1| multidrug ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 303 Score = 180 bits (456), Expect = 1e-46, Method: Compositional matrix adjust. Identities = 110/306 (35%), Positives = 176/306 (57%), Gaps = 12/306 (3%) Query: 7 LSVSKLHKAFGAGAQRVAIINDLSFALRPGECYGLLGPNGAGKTTTLRLCLGLATPDSGE 66 L ++ + K + Q + + +S A+ GE +GLLGPNGAGKTT + + GL DSG Sbjct: 4 LQINDIKKRY----QSLQALGGVSLAIEEGEFFGLLGPNGAGKTTLISIIAGLNRADSGT 59 Query: 67 IRLGGKAIPDQARAARMRVGVVPQADNLDPDFTVQENLLVFARYFGIADATMAQRIPELL 126 + + G + RAARM++GVVPQ DP FTV+E L + + YFG++ + + I E++ Sbjct: 60 VTIHGHDVVSDYRAARMKLGVVPQELVFDPFFTVRETLRMQSGYFGLS--SNDKWIDEVM 117 Query: 127 EFANLTAKKDARIAELSGGMKRRLTLARALVNDPDLIFMDEPTTGLDPQARHMIWERLKN 186 E +LT K D + LSGGMKRR+ +A+ALV+ P +I +DEPT G+D + R +W+ + Sbjct: 118 ENLDLTNKADTNMRALSGGMKRRVLVAQALVHKPPVIVLDEPTAGVDVELRQTLWQFIGR 177 Query: 187 LLARGKTILLTTHFMDEAERLCDRLAVIDHGKMIAEGSPPDLIAQHIEAEVLEIYGENAR 246 L G TI+LTTH+++EA+ LC+R+A++ GK++A S LI + ++++ + Sbjct: 178 LNREGHTIVLTTHYLEEAQALCNRIAMLKFGKVVALDSTEGLIRRISGSQMVLRLKRGSL 237 Query: 247 SWAEQHGRSHASRVEYSGETVFCYTNDAQA---LLATLKHVPGV--RYMHRPANLEDLFL 301 + +H + SG ND Q +LA L+ + A+LED+F+ Sbjct: 238 PEGLKSLVTHPEEL-LSGNKYTLRVNDYQEVEPILAALRAAGAEIDDLQLQQADLEDVFI 296 Query: 302 KLTGRE 307 ++ G E Sbjct: 297 QIMGDE 302 >gi|300311269|ref|YP_003775361.1| multidrug ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 913 Score = 148 bits (374), Expect = 5e-37, Method: Compositional matrix adjust. Identities = 81/205 (39%), Positives = 127/205 (61%), Gaps = 2/205 (0%) Query: 26 INDLSFALRPGECYGLLGPNGAGKTTTLRLCLGLATPDSGEIRLGGKAIPDQARAARMRV 85 ++ +SF +R GE +G LG NG GKTTT+++ GL SG RL G+ I + R RV Sbjct: 288 VDHVSFRIRRGEIFGFLGSNGCGKTTTMKMLTGLLPASSGRARLFGQEIDPRDVCLRKRV 347 Query: 86 GVVPQADNLDPDFTVQENLLVFARYFGIADATMAQRIPELLEFANLTAKKDARIAELSGG 145 G + Q+ +L D +V++NL + AR F + A + R+ E+ L D+ A+L G Sbjct: 348 GYMSQSFSLYKDLSVRQNLALHARLFHLPAAQIGPRVAEMARRFALMPVMDSLAADLPMG 407 Query: 146 MKRRLTLARALVNDPDLIFMDEPTTGLDPQARHMIWERLKNLLARGK-TILLTTHFMDEA 204 +++RL+LA A ++ P+++ +DEPT+G+DP AR W+ L L R K TI L+THFM+EA Sbjct: 408 IRQRLSLAVATIHGPEILILDEPTSGVDPVARDHFWQLLVTLSRRDKVTIFLSTHFMNEA 467 Query: 205 ERLCDRLAVIDHGKMIAEGSPPDLI 229 R CDR++++ GK++ +P L+ Sbjct: 468 LR-CDRMSMMHAGKVLDSDTPAALM 491 Score = 130 bits (328), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 83/204 (40%), Positives = 116/204 (56%), Gaps = 8/204 (3%) Query: 35 PGECY-GLLGPNGAGKTTTLRLCLGLATPDSGEIRLGGKAIPDQAR--AARMRVGVVPQA 91 PG C GL+GP+G GK+T L L G +G I + G I A A + +PQ Sbjct: 31 PGGCMAGLIGPDGVGKSTLLSLIAGARRIRAGRIDVLGGDIGQAAHRDAVGPCIAYMPQG 90 Query: 92 --DNLDPDFTVQENLLVFARYFGIADATMAQRIPELLEFANLTAKKDARIAELSGGMKRR 149 NL P +V+ENL AR FG QRI +L L +LSGGMK++ Sbjct: 91 LGKNLYPTLSVEENLQFIARLFGQDAQERRQRIDDLTHSTGLQPFLARPAGQLSGGMKQK 150 Query: 150 LTLARALVNDPDLIFMDEPTTGLDPQARHMIWERLKNLLAR--GKTILLTTHFMDEAERL 207 L+L AL++DPDL+ +DEPTTG+DP AR WE + L R G ++++ T +MDEA+ Sbjct: 151 LSLCCALIHDPDLLILDEPTTGVDPLARAQFWELIARLRQRHPGMSVIVATAYMDEAQAF 210 Query: 208 CDRLAVIDHGKMIAEGSPPDLIAQ 231 D LAV+D G+++A G+P L+A+ Sbjct: 211 -DWLAVMDQGQVLATGTPAALLAR 233 >gi|300311157|ref|YP_003775249.1| multidrug ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 923 Score = 139 bits (351), Expect = 3e-34, Method: Compositional matrix adjust. Identities = 77/215 (35%), Positives = 131/215 (60%), Gaps = 3/215 (1%) Query: 26 INDLSFALRPGECYGLLGPNGAGKTTTLRLCLGLATPDSGEIRLGGKAIPDQARAARMRV 85 ++ + F +R GE +G LG NG GK+TT+++ GL G+ L G+ I + A R RV Sbjct: 291 VDHVDFRIRRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGQAWLFGREIDPRDMATRARV 350 Query: 86 GVVPQADNLDPDFTVQENLLVFARYFGIADATMAQRIPELLEFANLTAKKDARIAELSGG 145 G + QA +L + +V++NL++ AR + + +A R E+ L D+ L G Sbjct: 351 GYMSQAFSLYGELSVRQNLVLHARLYRVPAEQIAARTDEMARRFGLLDVMDSLPESLPLG 410 Query: 146 MKRRLTLARALVNDPDLIFMDEPTTGLDPQARHMIWERLKNLLARGK-TILLTTHFMDEA 204 +++RL+LA A+V+ P+L+ +DEPT+G+DP AR + W+ + L + + TI ++THFM+EA Sbjct: 411 IRQRLSLAVAMVHKPELLILDEPTSGVDPIARDLFWQLMIGLARQDQVTIFISTHFMNEA 470 Query: 205 ERLCDRLAVIDHGKMIAEGSPPDLIAQHIEAEVLE 239 R CDR++++ G+++ +P L+ Q A+ LE Sbjct: 471 LR-CDRISLMHAGRVLVSATPQALMQQR-GAQTLE 503 Score = 121 bits (304), Expect = 7e-29, Method: Compositional matrix adjust. Identities = 77/221 (34%), Positives = 123/221 (55%), Gaps = 14/221 (6%) Query: 21 QRVAI-------INDLSFALRPGECYGLLGPNGAGKTTTLRLCLGLATPDSGEIRLGGKA 73 QRVA+ ++ +S + G GL+GP+G GK++ L L G GE+ + G Sbjct: 14 QRVALRYGKTVALDAVSLDIPAGCMVGLIGPDGVGKSSLLSLVAGARALQQGELAVLGGD 73 Query: 74 IPDQA--RAARMRVGVVPQA--DNLDPDFTVQENLLVFARYFGIADATMAQRIPELLEFA 129 + Q + A R+ +PQ NL P +V+ENL F R FG + +RI EL Sbjct: 74 MRQQRHRQQACPRIAYMPQGLGKNLYPTLSVEENLQFFGRLFGQDASERRRRIDELTTST 133 Query: 130 NLTAKKDARIAELSGGMKRRLTLARALVNDPDLIFMDEPTTGLDPQARHMIWERLKNLLA 189 L +LSGGMK++L L +L++DPDL+ +DEPTTG+DP +R W+ + ++ Sbjct: 134 GLDKFLARPAGKLSGGMKQKLGLCCSLIHDPDLLILDEPTTGVDPLSRAQFWDLIAHIRQ 193 Query: 190 R--GKTILLTTHFMDEAERLCDRLAVIDHGKMIAEGSPPDL 228 + G ++++ T +M+EA+R D L +D G+++A G+P L Sbjct: 194 QRAGMSVIVATAYMEEADRF-DWLVAMDEGRVLATGTPVQL 233 >gi|300310327|ref|YP_003774419.1| metal ion ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 345 Score = 134 bits (338), Expect = 8e-33, Method: Compositional matrix adjust. Identities = 80/231 (34%), Positives = 132/231 (57%), Gaps = 9/231 (3%) Query: 6 ILSVSKLHKAFGAGAQRVAIINDLSFALRPGECYGLLGPNGAGKTTTLRLCLGLATPDSG 65 ++ + + + FG V + + ++R GE +G++G +GAGK+T +R L P SG Sbjct: 1 MIEIQAVTQRFG----NVEAVRKVDLSIRKGEIFGIIGRSGAGKSTLVRTLNLLNRPTSG 56 Query: 66 EIRLGGKAIPD----QARAARMRVGVVPQADNLDPDFTVQENLLVFARYFGIADATMAQR 121 I L G+ + Q R AR +G++ Q NL +V +N+ + G + A +A + Sbjct: 57 RIVLDGQDLTSLSAAQLREARRGIGMIFQHFNLLSSRSVYDNIALPLELAGKSKAEIAAK 116 Query: 122 IPELLEFANLTAKKDARIAELSGGMKRRLTLARALVNDPDLIFMDEPTTGLDPQARHMIW 181 + LLE LTA +D A++SGG K+R+ +ARAL NDP ++ DE T+ LDP+ I Sbjct: 117 VEPLLELVGLTALRDRYPAQISGGQKQRVGIARALANDPKVLLSDEATSALDPETTRSIL 176 Query: 182 ERLKNLLAR-GKTILLTTHFMDEAERLCDRLAVIDHGKMIAEGSPPDLIAQ 231 E L+ + G TI+L TH M+ +++CDR+AV++ G++I +G D+ Q Sbjct: 177 ELLRKINKELGLTIVLITHQMEVIKQICDRVAVMEAGQVIEQGEVLDVFRQ 227 >gi|300313259|ref|YP_003777351.1| ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 247 Score = 132 bits (333), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 83/235 (35%), Positives = 130/235 (55%), Gaps = 11/235 (4%) Query: 5 SILSVSKLHKAFGAGAQRVAIINDLSFALRPGECYGLLGPNGAGKTTTLRLCLGLATPDS 64 S L V L K++GA ++ D+S +R GE GLLGPNGAGKTT+ + +GL D Sbjct: 4 SSLVVRGLKKSYGARQ----VVRDVSMEVRSGEVVGLLGPNGAGKTTSFYMIVGLVPSDG 59 Query: 65 GEIRLGGKAIPDQARAARMRVGV--VPQADNLDPDFTVQENL---LVFARYFG--IADAT 117 GEI L G+ I A R +G+ +PQ ++ TV++N+ L + G ++ Sbjct: 60 GEIDLDGEPISRLAIHKRATLGLSYLPQEASVFRKLTVEDNIRAVLELRQEDGKPLSRDE 119 Query: 118 MAQRIPELLEFANLTAKKDARIAELSGGMKRRLTLARALVNDPDLIFMDEPTTGLDPQAR 177 + R+ LL + ++++ LSGG +RR+ +ARAL DP + +DEP G+DP A Sbjct: 120 IEGRLNNLLHELQIEKLRESQALSLSGGERRRVEIARALATDPRFVLLDEPFAGIDPIAV 179 Query: 178 HMIWERLKNLLARGKTILLTTHFMDEAERLCDRLAVIDHGKMIAEGSPPDLIAQH 232 I ++ L RG +L+T H + E +CDR +I+ G ++A G+P ++IA Sbjct: 180 IEIQRIVRFLKERGIGVLITDHNVRETLGICDRAYIINQGTVLASGNPEEIIANE 234 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,032,179 Number of extensions: 45142 Number of successful extensions: 530 Number of sequences better than 1.0e-08: 134 Number of HSP's gapped: 318 Number of HSP's successfully gapped: 153 Length of query: 310 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 225 Effective length of database: 1,217,395 Effective search space: 273913875 Effective search space used: 273913875 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)