BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0281.p (400 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309490|ref|YP_003773582.1| branched-chain amino acid ABC t... 743 0.0 gi|300314071|ref|YP_003778163.1| amino acid ABC transporter peri... 554 e-159 gi|300312339|ref|YP_003776431.1| branched-chain amino acid ABC t... 506 e-144 gi|300312372|ref|YP_003776464.1| branched-chain amino acid ABC t... 357 e-100 gi|300313931|ref|YP_003778023.1| branched-chain amino acid ABC t... 102 4e-23 >gi|300309490|ref|YP_003773582.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 743 bits (1919), Expect = 0.0, Method: Compositional matrix adjust. Identities = 360/404 (89%), Positives = 378/404 (93%), Gaps = 4/404 (0%) Query: 1 LKKFLKMAALSAAMCA----AFSGNANAAGDTIKIGFITDLSGTYADMDGQGGIEAIKMA 56 + F++ AALSA CA A +A AAGDTIKIGFITD+SGTYAD DGQGG+EAI+MA Sbjct: 1 MTPFIRRAALSATACALLGAAILPSAQAAGDTIKIGFITDMSGTYADFDGQGGVEAIRMA 60 Query: 57 IADAGGTINGKKVEFVFADHQNKADIAANKAREWFDRDGVDMLVGGVNSAASLAMGKVAG 116 IADAGG INGKKVE VFADHQNKADIAANKAREWFDRDGVDMLVGGVNSAASLAMGKVAG Sbjct: 61 IADAGGAINGKKVELVFADHQNKADIAANKAREWFDRDGVDMLVGGVNSAASLAMGKVAG 120 Query: 117 EKKKVFISVGAGTTRQTNEECNAYTVQYAYDTTALARGTGAAITKQGGKSWYFVTADYAF 176 EKKKVFISVGAGTTRQTNEECNAYT+QYAYDTTALARGTGAAIT+QGGKSWYF+TADYAF Sbjct: 121 EKKKVFISVGAGTTRQTNEECNAYTIQYAYDTTALARGTGAAITRQGGKSWYFLTADYAF 180 Query: 177 GTSLEKDTSDVVKAGGGTVVGQVRAPLATSDFSSFLLQAQSSKAQILGLAVAGGDVINAI 236 GTSLEKDTS+VVK+ GG VVG R PLATSDFSSFLLQAQSSKAQILGLAVAGGDVINAI Sbjct: 181 GTSLEKDTSEVVKSNGGNVVGATRVPLATSDFSSFLLQAQSSKAQILGLAVAGGDVINAI 240 Query: 237 KAANEFGVNKTMKLAGLLIFITDTHSLGLQVTQGMYLTDGWYWDMNDQARAWGNRYFGKM 296 KAANEFGVNKTMKLAGLLIFI DTHSLGL +TQGMYLTDGWYWD+NDQ RAW NRYF KM Sbjct: 241 KAANEFGVNKTMKLAGLLIFINDTHSLGLSLTQGMYLTDGWYWDLNDQTRAWANRYFMKM 300 Query: 297 KKMPTMFQAGDASAVGQYLKAVKAVGSTDADKVMDYLRKNKINDFFTSNGVVRPDGRMVH 356 KKMP+MFQAGDASAVGQYLKAVKAVGSTDADKVMDYLRKN+INDFFTSNGVVRPDGRMVH Sbjct: 301 KKMPSMFQAGDASAVGQYLKAVKAVGSTDADKVMDYLRKNRINDFFTSNGVVRPDGRMVH 360 Query: 357 DMYLMEVKKPSESKRPWDYYKLVQTIPGDQAYMTKAESKCSLWK 400 DMYLMEVKKPSESKRPWDYYKLVQTIPG+QAYMTKAESKC+LWK Sbjct: 361 DMYLMEVKKPSESKRPWDYYKLVQTIPGEQAYMTKAESKCALWK 404 >gi|300314071|ref|YP_003778163.1| amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 402 Score = 554 bits (1428), Expect = e-159, Method: Compositional matrix adjust. Identities = 260/376 (69%), Positives = 307/376 (81%) Query: 25 AGDTIKIGFITDLSGTYADMDGQGGIEAIKMAIADAGGTINGKKVEFVFADHQNKADIAA 84 +GD IKIGFITD+SG Y+D+DGQGG EAIKMAIAD GG INGKK+E + ADHQNKAD+AA Sbjct: 27 SGDVIKIGFITDISGVYSDIDGQGGAEAIKMAIADMGGAINGKKIELLVADHQNKADVAA 86 Query: 85 NKAREWFDRDGVDMLVGGVNSAASLAMGKVAGEKKKVFISVGAGTTRQTNEECNAYTVQY 144 +KAREWFD+ GVDML+GG NS +LA KVA EKKKV++ +GAG+ R TNEEC+ YTV Y Sbjct: 87 SKAREWFDQQGVDMLIGGTNSGTALASAKVAAEKKKVYMQIGAGSARLTNEECSPYTVHY 146 Query: 145 AYDTTALARGTGAAITKQGGKSWYFVTADYAFGTSLEKDTSDVVKAGGGTVVGQVRAPLA 204 AYDT ALA+GTG+A+ KQGGKSW+F+TADYAFG SLE DT++VVKA GGTVVG V+ PL Sbjct: 147 AYDTVALAKGTGSAVVKQGGKSWFFLTADYAFGHSLESDTANVVKASGGTVVGAVKHPLG 206 Query: 205 TSDFSSFLLQAQSSKAQILGLAVAGGDVINAIKAANEFGVNKTMKLAGLLIFITDTHSLG 264 SDFSSFLLQAQSSKAQI+GLA AGGD IN++KAANEFG+ KT KLAGLL+FI D HSL Sbjct: 207 ASDFSSFLLQAQSSKAQIIGLANAGGDFINSVKAANEFGITKTAKLAGLLVFINDVHSLT 266 Query: 265 LQVTQGMYLTDGWYWDMNDQARAWGNRYFGKMKKMPTMFQAGDASAVGQYLKAVKAVGST 324 L+ T+G+YLTD WYWD N++ RA+ RYF KKMP+ QA D SA QYLK VK +G+ Sbjct: 267 LKNTEGLYLTDSWYWDANEETRAFAKRYFAVRKKMPSSLQAADYSAALQYLKVVKEIGTD 326 Query: 325 DADKVMDYLRKNKINDFFTSNGVVRPDGRMVHDMYLMEVKKPSESKRPWDYYKLVQTIPG 384 D+DKVM LRK K ND + NG +R DGR++HDMYL +VK SESK PWDYYKLVQTIP Sbjct: 327 DSDKVMAALRKAKFNDMYQKNGYLRADGRVMHDMYLYQVKAQSESKYPWDYYKLVQTIPA 386 Query: 385 DQAYMTKAESKCSLWK 400 +QA+ TKAESKC+ WK Sbjct: 387 EQAWTTKAESKCAAWK 402 >gi|300312339|ref|YP_003776431.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 506 bits (1304), Expect = e-144, Method: Compositional matrix adjust. Identities = 241/398 (60%), Positives = 305/398 (76%), Gaps = 3/398 (0%) Query: 5 LKMAALSAAMCAAFSGNANA--AGDTIKIGFITDLSGTYADMDGQGGIEAIKMAIADAGG 62 L+ A + A+ FSG A A + D ++IGFITD+SG YAD DG GG+EAI+MA+AD GG Sbjct: 7 LRRLAAACAIAGIFSGAALAQVSNDAVRIGFITDMSGPYADTDGLGGLEAIRMAVADFGG 66 Query: 63 TINGKKVEFVFADHQNKADIAANKAREWFDRDGVDMLVGGVNSAASLAMGKVAGEKKKVF 122 T+ GK +E + ADHQNKADIAA +AREW D G+DML+GGVNSA +L+M KV EKK+V+ Sbjct: 67 TVLGKPIEVISADHQNKADIAATRAREWADERGLDMLIGGVNSATALSMNKVMAEKKRVY 126 Query: 123 ISVGAGTTRQTNEECNAYTVQYAYDTTALARGTGAAITKQGGKSWYFVTADYAFGTSLEK 182 I++GAGT R TNEEC YTV Y YDT ALA+GT +A+ KQGGKSW+F+ ADYAFG SL Sbjct: 127 INIGAGTARLTNEECTPYTVHYEYDTVALAKGTASAVIKQGGKSWFFLVADYAFGHSLAN 186 Query: 183 DTSDVVKAGGGTVVGQVRAPLATSDFSSFLLQAQSSKAQILGLAVAGGDVINAIKAANEF 242 DTS VVKA GGTVVG ++ P +SD SSFLLQAQ+SKAQILGLA AG D +N+IKAA EF Sbjct: 187 DTSAVVKASGGTVVGSIKHPPQSSDLSSFLLQAQASKAQILGLANAGEDTVNSIKAAKEF 246 Query: 243 GVNKTMKLAGLLIFITDTHSLGLQVTQGMYLTDGWYWDMNDQARAWGNRYFGKMKKMPTM 302 GV KTMKL GLL+ I D H+LGL +G+ +TD WYWD ++ +R + R+F KMKKMP+ Sbjct: 247 GVTKTMKLVGLLMVINDIHALGLANAEGLMMTDSWYWDKDEPSRKFAERFFQKMKKMPST 306 Query: 303 FQAGDASAVGQYLKAVKAVGSTDADKVMDYLRKNKINDFFTSNGVVRPDGRMVHDMYLME 362 QA SA YLKAV+A+G+ D+ KVM L+K +I+DF+ + G +R DGR +HDMYL + Sbjct: 307 HQAAAYSATTTYLKAVQAIGTDDSTKVMAQLKKTRIDDFY-NKGSIRADGRNIHDMYLYQ 365 Query: 363 VKKPSESKRPWDYYKLVQTIPGDQAYMTKAESKCSLWK 400 VK P+ESK+PWDY KL++TIPG+QA+ + A+SKCSL K Sbjct: 366 VKSPAESKKPWDYLKLLETIPGEQAFTSVADSKCSLLK 403 >gi|300312372|ref|YP_003776464.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 410 Score = 357 bits (916), Expect = e-100, Method: Compositional matrix adjust. Identities = 192/395 (48%), Positives = 254/395 (64%), Gaps = 8/395 (2%) Query: 9 ALSAAMCAAFSGNANAAGDTIKIGFITDLSGTYADMDGQGGIEAIKMAIADA-GGTINGK 67 +L+A A++ +AN T+KIG +TD+SG Y+ M GQG + A KMA+ D G Sbjct: 20 SLAATAIPAWAQDAN----TVKIGVLTDMSGPYSAMGGQGSVVAAKMAVEDCLKNECKGM 75 Query: 68 KVEFVFADHQNKADIAANKAREWFDRDGVDMLVGGVNSAASLAMGKVAGEKKKVFISVGA 127 K+E + AD+QNK D+ +AREW DRD V+ + NS+ +LA+ K+ +K + + G Sbjct: 76 KIEILSADNQNKPDVGVTRAREWLDRDKVEAIADLTNSSVALAVQKLIKDKGGIAMYSGP 135 Query: 128 GTTRQTNEECNAYTVQYAYDTTALARGTGAAITKQGGKSWYFVTADYAFGTSLEKDTSDV 187 T T EC + +DT + A G AA+TK G KSWYFVT DYAFG SLEKD SDV Sbjct: 136 ATGALTTAECAPNGFHWMFDTYSQAAGAAAALTKLGNKSWYFVTVDYAFGHSLEKDASDV 195 Query: 188 VKAGGGTVVGQVRAPLATSDFSSFLLQAQSSKAQILGLAVAGGDVINAIKAANEFGV-NK 246 VKA GGTVVG VR PL SDFSSFLLQAQ+SKAQ++GLA G DV+NAIK A F V K Sbjct: 196 VKANGGTVVGSVRHPLNASDFSSFLLQAQASKAQVIGLADGGTDVVNAIKQARSFAVGGK 255 Query: 247 TMKLAGLLIFITDTHSLGLQVTQGMYLTDGWYWDMNDQARAWGNRYFGKMKKM-PTMFQA 305 +L LL+F++D H+LGL+ QG+ TDG+YWD ++Q RA+ R+ + K + PTM QA Sbjct: 256 DQRLVALLVFLSDVHALGLEAAQGLVYTDGFYWDYDEQTRAFSKRFESQFKNIKPTMVQA 315 Query: 306 GDASAVGQYLKAVKAVGSTDADKVMDYLRKNKINDFFTSNGVVRPDGRMVHDMYLMEVKK 365 G S+V Q+LKA A ++D V +R+ I D N +RPDGR++HDMYL +VK Sbjct: 316 GVYSSVYQFLKARAAAKTSDWKVVAQKMREIPIADAVMRNASIRPDGRVIHDMYLYQVKT 375 Query: 366 PSESKRPWDYYKLVQTIPGDQAYMTKAESKCSLWK 400 P+ESK PWDY KL+ IP QA+ ++ C L K Sbjct: 376 PAESKGPWDYLKLLSVIPAQQAF-RPMDAACPLVK 409 >gi|300313931|ref|YP_003778023.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 397 Score = 102 bits (255), Expect = 4e-23, Method: Compositional matrix adjust. Identities = 108/409 (26%), Positives = 181/409 (44%), Gaps = 28/409 (6%) Query: 2 KKFLKMAALSAAMCAAFSGNANAAGDTIKIGFITDLSGTYADMDGQGGIEAIKMAIADAG 61 + FLK++AL+A ++ + A ++I G +SG +A +G+ +K+A+ G Sbjct: 5 RHFLKLSALAAPGALSYH-DVFAQAESIAFGCPVPMSGPFA-ANGKFADLGMKLALQKYG 62 Query: 62 GTINGKKVEFVFADHQNKADIAANKAREWFDRDGVDMLVGGVNSAASLAMGKVAGEKKKV 121 + GK +V D + K A K +E ++ G GG+ S +LAMGK A + V Sbjct: 63 KVL-GKSTSYVNLDTEGKPATAVRKMQESIEK-GTRFFAGGILSGEALAMGKEADKAGAV 120 Query: 122 FISVGAGTTRQTNEECNAYTVQYAYDTTALARGTGAAITKQ--GGKSWYFVTADYAFGTS 179 FI+ AG T +CN T +++ T T + ++ K WY +T Y FG Sbjct: 121 FITT-AGADEITGSDCNRATFRWSVPTFGAIEQTVRPLLEKMPNAKRWYTITPQYVFGEG 179 Query: 180 LEKDTSDVVKAGGGTVVGQVRAPLATSDFSSFLLQAQSSKAQILGLAVAGGDVINAIKAA 239 L ++ K G VG L +FS +L A ++K +L L G + ++ A Sbjct: 180 LLSAAKNIFKEKGIEHVGNSYHSLTEKEFSGYLTNAIAAKPDVLLLLNFGSQSSDTLRQA 239 Query: 240 NEFGV--NKTMKLA---GLLIFITDTHSLGLQVTQGMYLTDGWYWDMNDQARAWGNRYFG 294 FG+ N T+ +A GL F T LG + G+Y YW D A N+ F Sbjct: 240 VSFGMKNNCTILMAWASGLEQFET----LGADICDGVYF-GAQYWHAID---APFNKEFV 291 Query: 295 K-----MKKMPTMFQAGDASAVGQYLKAVKAVGSTDADKVMDYLRKNKINDFFTSNGVVR 349 K +K P AG L A+ STD V+ + K S + + Sbjct: 292 KLVNDNLKIDPNYSLAGSYICTKIILDAIIKANSTDPKAVIAAMEGMKYEGLTGSEEIRK 351 Query: 350 PDGRMVHDMYLMEVKKPSESKRPWDYYKLVQTIPGDQAYMTKAESKCSL 398 D +++ + YL++ K ++ DY +V + ++++ +++C L Sbjct: 352 GDHQVIKNYYLLKGKAKAKMANKNDYADIVSS---GKSFLPLEKTQCKL 397 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.131 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,209,959 Number of extensions: 51718 Number of successful extensions: 206 Number of sequences better than 1.0e-08: 12 Number of HSP's gapped: 198 Number of HSP's successfully gapped: 12 Length of query: 400 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 313 Effective length of database: 1,207,925 Effective search space: 378080525 Effective search space used: 378080525 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)