BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0264.p (154 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309501|ref|YP_003773593.1| hypothetical protein Hsero_0159... 214 2e-57 >gi|300309501|ref|YP_003773593.1| hypothetical protein Hsero_0159 [Herbaspirillum seropedicae SmR1] Length = 149 Score = 214 bits (545), Expect = 2e-57, Method: Compositional matrix adjust. Identities = 106/149 (71%), Positives = 127/149 (85%), Gaps = 2/149 (1%) Query: 6 RASLILSTLILGSAAAAPAFALDFKSVGAAPAVLYDAPSEKGRRVFVAPRGMPVEVVLTY 65 R LILS +LG+ A A ALDFKSVGAAPA++YDAPSEKGRRV+VAPRGMPVEVVLTY Sbjct: 3 RLPLILSATLLGAFLAKDALALDFKSVGAAPAIMYDAPSEKGRRVYVAPRGMPVEVVLTY 62 Query: 66 GEWSKVRDAAGTLSWVMSKSLTTKRNVVVSVANAKVHAAADDNSATVFSADKNVLLELTD 125 GEWSKVRDAAGTLSWV SK+LT KR +VVS ANA+V+ AAD++S VF+ADK+VLLE+ + Sbjct: 63 GEWSKVRDAAGTLSWVSSKALTPKRMLVVSAANARVYNAADESSPVVFTADKSVLLEMLE 122 Query: 126 ATPVSGGWIKVKHRDGQSGFVKASEVWGD 154 + + GW+KV+HRDGQ+GFVKA +VWGD Sbjct: 123 SP--NNGWVKVRHRDGQTGFVKAGDVWGD 149 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.128 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 444,880 Number of extensions: 15334 Number of successful extensions: 59 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 58 Number of HSP's successfully gapped: 1 Length of query: 154 Length of database: 1,619,870 Length adjustment: 77 Effective length of query: 77 Effective length of database: 1,255,275 Effective search space: 96656175 Effective search space used: 96656175 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 126 (53.1 bits)