BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0255.p (441 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310313|ref|YP_003774405.1| acyl-CoA dehydrogenase [Herbasp... 491 e-140 gi|300313303|ref|YP_003777395.1| acyl-CoA dehydrogenase [Herbasp... 85 1e-17 gi|300309596|ref|YP_003773688.1| acyl-CoA dehydrogenase [Herbasp... 81 1e-16 gi|300310835|ref|YP_003774927.1| acyl-CoA dehydrogenase [Herbasp... 81 2e-16 gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbasp... 80 3e-16 >gi|300310313|ref|YP_003774405.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 550 Score = 491 bits (1263), Expect = e-140, Method: Compositional matrix adjust. Identities = 249/327 (76%), Positives = 277/327 (84%), Gaps = 3/327 (0%) Query: 9 PMCDAHMVLARTDNGLSCFFVPRWRPDGHKNGVLVQRLKDKLGNRSNSSSEVEFEDAYGV 68 PMCDAH++LART+NGLSCFFVPRW PDG +N +L+QRLKDKLGNRSNSSSEVEFE+A G+ Sbjct: 222 PMCDAHLMLARTENGLSCFFVPRWLPDGTRNPILIQRLKDKLGNRSNSSSEVEFEEAMGI 281 Query: 69 MVGDEGRGIPTIIEMANHTRLDCVIGSAGLMRHALVQAIHHARHRSAFGKHLAEQALMRN 128 MVGDEGRGIPTIIEMANHTRLDC+IGSA LMR ALVQAIHHARHRSAFG+ LAEQ LMR Sbjct: 282 MVGDEGRGIPTIIEMANHTRLDCIIGSAALMRQALVQAIHHARHRSAFGRRLAEQPLMRA 341 Query: 129 VLADLALESEAATLLMLRLASAFEAPADPLQRAWRRIVTPAAKFWICKRALEFTGECMEV 188 VLADLALESEAAT+LMLR+A AF+AP DPLQRAW+RI+TPAAKFWICKR LEFTGECMEV Sbjct: 342 VLADLALESEAATMLMLRVAHAFDAPDDPLQRAWKRIITPAAKFWICKRTLEFTGECMEV 401 Query: 189 WGGNGYVETGPMARMYREAPVNSIWEGSGNVMCLDVLRAMEREPDACLLLLDELQQVADG 248 WGGNGYVET PMAR+YREAPVNSIWEGSGNVMCLDVLRAM RE LLL EL A G Sbjct: 402 WGGNGYVETAPMARLYREAPVNSIWEGSGNVMCLDVLRAMARETQGLALLLLELDDAAAG 461 Query: 249 HEVLQAPLHALKQMMTLAPEQREMLARRLVQQLVLAVQGALMLQHAPPAMAEAFVLSRGD 308 H L+ + ALKQM+ E+RE ARRLVQQLVLA+QG LML+HAP A+AF+ SR Sbjct: 462 HPALRQQVDALKQMLAAPEEEREAGARRLVQQLVLALQGMLMLRHAPAGSAQAFLESRSQ 521 Query: 309 AANGRVYGTLPAGTADAASLQKSILAR 335 A GRVYGTL G+A A+ Q++IL R Sbjct: 522 ADGGRVYGTL--GSASLAA-QEAILQR 545 >gi|300313303|ref|YP_003777395.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 591 Score = 84.7 bits (208), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 66/232 (28%), Positives = 106/232 (45%), Gaps = 20/232 (8%) Query: 14 HMVLAR------TDNGLSCFFVPRWRPDGHKNGVLVQRLKDKLGNRSNSSSEVEFEDAYG 67 H+VLAR GLS F PR DG +NGV ++ K+G +++ + FE A Sbjct: 212 HLVLARLPDAPAGSKGLSLFLAPRVLDDGSRNGVHCTGIEHKMGIHGSATCSLHFEQAQA 271 Query: 68 VMVGDEGRGIPTIIEMANHTRLDCVIGSAGLMRHALVQAIHHARHR-------SAFGKHL 120 +VG+ GRG+ + M N RL GL A +A +AR R S+ + Sbjct: 272 WLVGEAGRGLAAMFVMMNAARLHVGAQGLGLAEAAYQRASAYARERRQSRAPGSSGNDLI 331 Query: 121 AEQALMRNVLADLALESEAATLLM----LRLASAFEAPADPLQ-RAWRRI--VTPAAKFW 173 ++ +L + E A LL L L A P L+ R R+ +TP K + Sbjct: 332 VHHPAVQKLLMEQRCRIEGARLLTCWAGLLLDLAEHDPEAALRSRHHERLEFITPVIKAF 391 Query: 174 ICKRALEFTGECMEVWGGNGYVETGPMARMYREAPVNSIWEGSGNVMCLDVL 225 + + + ++V+GG+GYV + + R+A + I+EG+ + +D L Sbjct: 392 LTDQGFQCASRALQVFGGHGYVVETGIEQFMRDARITMIYEGTNEIQAIDFL 443 >gi|300309596|ref|YP_003773688.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 596 Score = 81.3 bits (199), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 63/242 (26%), Positives = 113/242 (46%), Gaps = 30/242 (12%) Query: 14 HMVLAR------TDNGLSCFFVPRWRPD-----GHKNGVLVQRLKDKLGNRSNSSSEVEF 62 H+VLAR G+S F VP++ P+ G +N + +++K+G N++ ++ Sbjct: 213 HLVLARLPDAPEGSKGISLFLVPKFLPNADGSLGARNPIFCGAIEEKMGIHGNATCQMNL 272 Query: 63 EDAYGVMVGDEGRGIPTIIEMANHTRLDCVIGSAGL----MRHALVQAIHHARHRSAFGK 118 + A G ++G +G+ + N RL + GL ++ALV A + RS G Sbjct: 273 DGATGWLIGQPHKGLQAMFVFMNAARLGVGMQGLGLTEVAYQNALVYAKDRLQMRSLSGI 332 Query: 119 HLAEQAL--------MRNVLADLALESEAA----TLLMLRLASAFEAPADPLQRAWRRIV 166 A++ +R +L +E + + L L P + +++ IV Sbjct: 333 KAADKPADPIIVHPDVRRMLLTAKAFAEGGRAFTSYVALELDKELTHPDEQVRKDSADIV 392 Query: 167 ---TPAAKFWICKRALEFTGECMEVWGGNGYVETGPMARMYREAPVNSIWEGSGNVMCLD 223 TP K ++ A T EC++V+GG+GY+ M + R+A +N I+EG+ + LD Sbjct: 393 ALLTPVVKAFLTDNAWMATSECLQVYGGHGYIAEWGMEQYVRDARINMIYEGTNTIQSLD 452 Query: 224 VL 225 +L Sbjct: 453 LL 454 >gi|300310835|ref|YP_003774927.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 594 Score = 80.9 bits (198), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 68/242 (28%), Positives = 116/242 (47%), Gaps = 31/242 (12%) Query: 14 HMVLARTDN------GLSCFFVPRWRPD-----GHKNGVLVQRLKDKLGNRSNSSSEVEF 62 H+VLART N G+S F VP++ + G +N V ++ KLG +++ ++ ++F Sbjct: 209 HLVLARTPNAPEGVKGISLFIVPKFLVNADGSLGARNDVHCVSIEHKLGIKASPTAVLQF 268 Query: 63 ED---AYGVMVGDEGRGIPTIIEMANHTRLDCVIGSAGLMRHALVQAIHHARHRSAFGKH 119 D A G +VG+E RG+ + M N R I + A +A+ +AR R + Sbjct: 269 GDHGGAIGTLVGEENRGLEYMFIMMNAARFAVGIQGLSVSERAYQKAVAYARDRIQ-SRD 327 Query: 120 LA---------EQALMRNVLADLALESEAATLLMLRLASAFEA---PADPLQR----AWR 163 LA Q +R +L + + EAA L A+ +A D R A+ Sbjct: 328 LAGSPGPVAIIHQPDVRRMLMSMRAQIEAARALSYVAAATSDAAHFSDDEATRKSNQAFY 387 Query: 164 RIVTPAAKFWICKRALEFTGECMEVWGGNGYVETGPMARMYREAPVNSIWEGSGNVMCLD 223 + P K WI + +++ T ++V GG G++E A+ YR+A + I+EG+ + D Sbjct: 388 EYLVPIVKGWITEMSIDVTSTGVQVHGGMGFIEETGAAQYYRDARILPIYEGTTAIQAND 447 Query: 224 VL 225 ++ Sbjct: 448 LV 449 >gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 80.1 bits (196), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 59/222 (26%), Positives = 105/222 (47%), Gaps = 19/222 (8%) Query: 15 MVLARTD-----NGLSCFFVPRWRPDGHKNGVLVQRLKDKLGNRSNSSSEVEFEDAY--- 66 +V A TD G+SCF VP P G +V R ++K+G ++ + ++ ++ Sbjct: 165 IVFAVTDRSAGKKGISCFLVPCDTP-----GFVVGRSEEKMGQHASDTVQIMLDNCRVPA 219 Query: 67 GVMVGDEGRGIPTIIEMANHTRLDCVIGSAGLMRHALVQAIHHARHRSAFGKHLAEQALM 126 ++G EG G + R+ S G+ R A A+ +AR R +FG + E + Sbjct: 220 TALLGKEGEGYRIALSNLEAGRIGIAAQSVGMARAAFEAAVSYARQRESFGVPIIEHQAV 279 Query: 127 RNVLADLALESEAATLLMLRLASAFEAPADPLQRAWRRIVTPAAKFWICKRALEFTGECM 186 LAD+ +AA L++ R A + L+ A AK + + A + + + Sbjct: 280 NFRLADMNTLLDAARLMVWRAAQLKDQGRPCLKEA------SMAKMFASEAAEKIASDAI 333 Query: 187 EVWGGNGYVETGPMARMYREAPVNSIWEGSGNVMCLDVLRAM 228 ++ GG GY P+ R+YR+ + I+EG+ ++ L + RA+ Sbjct: 334 QIHGGVGYTSDFPVERIYRDVRICQIYEGANDIQRLVIGRAI 375 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.132 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,347,232 Number of extensions: 54055 Number of successful extensions: 217 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 211 Number of HSP's successfully gapped: 7 Length of query: 441 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 353 Effective length of database: 1,203,190 Effective search space: 424726070 Effective search space used: 424726070 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 132 (55.5 bits)