BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0244.p (452 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310319|ref|YP_003774411.1| TonB-dependent siderophore rece... 600 e-173 gi|300309379|ref|YP_003773471.1| TonB-dependent ferric sideropho... 146 3e-36 gi|300311911|ref|YP_003776003.1| TonB-dependent siderophore rece... 136 4e-33 gi|300309869|ref|YP_003773961.1| TonB-dependent ferric sideropho... 130 3e-31 gi|300311660|ref|YP_003775752.1| TonB-dependent receptor protein... 83 4e-17 >gi|300310319|ref|YP_003774411.1| TonB-dependent siderophore receptor protein [Herbaspirillum seropedicae SmR1] Length = 723 Score = 600 bits (1548), Expect = e-173, Method: Compositional matrix adjust. Identities = 285/447 (63%), Positives = 354/447 (79%), Gaps = 6/447 (1%) Query: 8 MLSPQPWVQPVRIDSLNFNTRFDLEFNDNWRGYITAGRSRAVIDDYLAFPYGSNVATVFS 67 ML+PQPW QPVR S+N R D EFN +WRGY+ AGRSRAVIDDYLAFPYGS S Sbjct: 281 MLTPQPWSQPVRDSSVNARLRIDTEFNADWRGYVQAGRSRAVIDDYLAFPYGSGNGA--S 338 Query: 68 PTFAANGDYDVYDYRSPDDTRRNDEAQAVVQGKFNMGALRHGLTLGLTMSRRVIDKSDGI 127 PTFAANGD+DVYDYR P+D RRNDE QAV GKF G ++H LTLG+ MSRR++DKSDGI Sbjct: 339 PTFAANGDFDVYDYRVPNDERRNDEVQAVAIGKFASGWVQHELTLGMAMSRRIVDKSDGI 398 Query: 128 SIPVGTGNIYSPAAAPLAPSG-ADIPASYRNLDSRQQAMLFTDRVQFGERWEVLAGGRQV 186 S+ VG GNIY+ P+ P+ A IPA+YRNLDSRQ+A+ F+DR+QF +RW+VLAGGRQV Sbjct: 399 SVLVGQGNIYN--GTPVLPAANAVIPAAYRNLDSRQKALFFSDRMQFSQRWQVLAGGRQV 456 Query: 187 WLKEKTYAATGATTRDTDRNQFLPQLALIYKPQVNMSLYGSYAETLSMGTAAAFWQTNYP 246 WL E+++ +TGA TRDT RN FLPQLALI+KP N+SLYGSYAETLS+G++A FW +N+P Sbjct: 457 WLNEQSFNSTGAVTRDTSRNLFLPQLALIFKPVQNLSLYGSYAETLSLGSSAPFWVSNFP 516 Query: 247 TILPPSVARQLETGIKYDWNKDLSFTAAIFRMKKAYEYPQPDGA-GGFKYVQQGKQTHTG 305 LPPSVARQLE G+KY+++ +L+ TAA+FR +KAYEY +PD + GF YVQQG+QT+TG Sbjct: 517 VTLPPSVARQLEAGVKYEFSPELALTAAVFRTRKAYEYQKPDNSPTGFTYVQQGQQTNTG 576 Query: 306 LELGAAGNVTKQLRLAASLAFIQARADGTGTPAYDDKQAVNVPRLRGALYADYALAALPG 365 +ELGA G +T +L + A+ ++ARA TGT +DD+Q VNVPRLR A+YADYA+ + G Sbjct: 577 VELGANGKLTPRLAIGAAATVLRARASDTGTARFDDQQVVNVPRLRTAVYADYAVPGMEG 636 Query: 366 LNLQGSWLYSGSKSATRDGSATVPSYHIFNTGLRYQTRMGGHPTTVRLTVDNVFDKRYWK 425 +N+ GSW YS SK ATRDGS +P+ H+FN GLRYQ R+ GH T+R +DN+FDKRYWK Sbjct: 637 VNVMGSWNYSASKPATRDGSVNLPAVHVFNAGLRYQLRVNGHAATLRANIDNLFDKRYWK 696 Query: 426 DTGESLGDSYLHLGAPRLARLSVQYDF 452 D G+ LGD Y+HLGAPR ARLS+QYDF Sbjct: 697 DAGQYLGDGYVHLGAPRTARLSLQYDF 723 >gi|300309379|ref|YP_003773471.1| TonB-dependent ferric siderophore receptor protein [Herbaspirillum seropedicae SmR1] Length = 790 Score = 146 bits (369), Expect = 3e-36, Method: Compositional matrix adjust. Identities = 124/430 (28%), Positives = 198/430 (46%), Gaps = 24/430 (5%) Query: 27 TRFDLEFNDNWRGYITAGRSRAVIDDYLAFPYGSNVATVFSPTFAANGDYDVYDYRSPDD 86 TR ++E ++ Y G+ R+ L P GS A+ A + Y D Sbjct: 381 TRVEVELSEQLSAYAAVGQRRSATQQNL--PTGSVDAS------GAGSVRNAYY----DT 428 Query: 87 TRRNDEAQAVVQGKFNMGALRHGLTLGLTMSRRVIDKSDGISIPVGTGNIYSPAAAPLAP 146 R A A ++ + GA+RHG++LG+T R+ + +S ++Y+PA P+ Sbjct: 429 ASRTLSADAGLRLQAATGAVRHGVSLGVTRLRQQAGNAYLVSADSSAYSLYAPATLPVIS 488 Query: 147 SGADIPASYRNLDSRQQAMLFTDRVQFGE-RWEVLAGGRQVWLKEKTY-AATGATTRDTD 204 + P + D ++ TD ++ E R ++L G R+ ++ ++ +GA + Sbjct: 489 AARTEP--RKAYDMTLDSLSLTDTLRLLEDRLQLLVGARRQSVRYDSFDTVSGAQLSSYE 546 Query: 205 RNQFLPQLALIYKPQVNMSLYGSYAETLSMGTAAAFWQTNYPTILPPSVARQLETGIKYD 264 + P L L+ KP +SLY + L+ GT N +L P +RQLE G K D Sbjct: 547 ASAISPLLGLVVKPVAGLSLYANTTSGLTRGTIVPTGLANAGQVLAPYRSRQLEAGAKLD 606 Query: 265 WNKDLSFTAAIFRMKK--AYEYPQPDGAGGFKYVQQGKQTHTGLELGAAGNVTKQLRLAA 322 W + ++ AA F++ A Q Y G+Q + GLEL A G + + LRL A Sbjct: 607 WGRVMT-QAAWFQIDNPSAVITTQASTPASSSYGYDGRQRNRGLELSAYGELMRGLRLLA 665 Query: 323 SLAFIQARADGTGTPAYDDKQAVNVPRLRGALYADYALAALPGLNLQGSWLYSGSKSATR 382 S F QAR T D + A VP L D+ A LPGL G +++G Sbjct: 666 SATFYQARLLRTANGLNDGRTAPGVPLRAANLSLDWEPAGLPGLAFSGRMIHTGPVYYDA 725 Query: 383 DGSATVPSYHIFNTGLRYQTRMGGHPTTVRLTVDNVFDKRYWKDTGESLGDSYLHLGAPR 442 + +PS+ ++ G+RY++R+G P RL V+N+F+ W L Y+ + APR Sbjct: 726 ANTLRLPSWTRWDLGVRYRSRLGERPVVWRLNVENLFNHSTWL-----LDKPYVTVAAPR 780 Query: 443 LARLSVQYDF 452 LS+Q D+ Sbjct: 781 TVLLSMQVDW 790 >gi|300311911|ref|YP_003776003.1| TonB-dependent siderophore receptor protein [Herbaspirillum seropedicae SmR1] Length = 733 Score = 136 bits (342), Expect = 4e-33, Method: Compositional matrix adjust. Identities = 115/384 (29%), Positives = 177/384 (46%), Gaps = 27/384 (7%) Query: 85 DDTRRNDEA--QAVVQGKFNMGALRHGLTLGLTM----SRRVIDKSDGISIPVGTGNIYS 138 D+ R++D A + V+ GA+ H L + SR S ++ N+Y+ Sbjct: 361 DNARQDDVATGEVGVRTHLQTGAVSHKLVAAASAYSLNSRNAYGLSPSYALAT---NLYN 417 Query: 139 P---AAAPLAPSGADIPASYRNLDSRQQAMLFTDRVQF-GERWEVLAGGRQVWLKEKTYA 194 P A L G + + + + + +D + F +R V G RQ LK TYA Sbjct: 418 PTNYAQPALTSLGNSLADPQTTIKTNLLSAVVSDTLGFYDDRVLVTVGVRQQQLKSDTYA 477 Query: 195 -ATGATTRDTDRNQFLPQLALIYKPQVNMSLYGSYAETLSMG-----TAAAFWQTNYPTI 248 T A T D++ P +++K +S+YG+Y E L G T AA NY + Sbjct: 478 YNTQANTAHYDQSATSPMAGVVFKLTKTLSVYGNYIEGLQQGKQITDTTAA----NYGQV 533 Query: 249 LPPSVARQLETGIKYDWNKDLSFTAAIFRMKKAYEYPQPDGAGGFKYVQQGKQTHTGLEL 308 L P ++Q E G+KY+ L AA F + Y D + GKQ + G+EL Sbjct: 534 LAPYKSKQKEVGVKYELGG-LGLGAAFFTTDRPLSYK--DAVTNIE-ATNGKQRNRGVEL 589 Query: 309 GAAGNVTKQLRLAASLAFIQARADGTGTPAYDDKQAVNVPRLRGALYADYALAALPGLNL 368 A G VTK +RL + L F+ A+ T + A + K A+ VPR++ + + + GL L Sbjct: 590 TAYGEVTKGVRLLSGLTFLDAKQVQTNSSANNGKYAIGVPRVQANAGLEVDVPGVRGLTL 649 Query: 369 QGSWLYSGSKSATRDGSATVPSYHIFNTGLRYQTRMGGHPTTVRLTVDNVFDKRYWKDTG 428 G Y+ ++ VPS+ ++ G RY T +GGH T+R ++DNV ++ YW+ G Sbjct: 650 NGRATYTSTQYVDAANQLEVPSWVRYDLGARYLTDIGGHAVTLRASIDNVANRNYWQSAG 709 Query: 429 ESLGDSYLHLGAPRLARLSVQYDF 452 + YL G PR SV F Sbjct: 710 GASNSGYLVAGNPRTFSTSVSVAF 733 >gi|300309869|ref|YP_003773961.1| TonB-dependent ferric siderophore receptor protein [Herbaspirillum seropedicae SmR1] Length = 725 Score = 130 bits (326), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 118/439 (26%), Positives = 192/439 (43%), Gaps = 31/439 (7%) Query: 18 VRIDSLNFNTRFDLEFNDNWRGYITAGRSRAVIDDYLAFPYGSNVATVFSPTFAANGDYD 77 ++ID TR + + + Y G +FP + + + T NG YD Sbjct: 314 LQIDDKVVATRLEYDLTEQLTAYGAIGYHEGATAQ--SFP-SATITGLDGATSVRNGYYD 370 Query: 78 VYDYRSPDDTRRNDEAQAVVQGKFNMGALRHGLTLGLTMSRRVIDKSDG----ISIPVGT 133 + D ++ +F G ++H L+ M+ +D+ ++ Sbjct: 371 QKVVSTSADVG--------LRARFATGPVQHTLS----MAANTLDQQTSYFYFVNATGVA 418 Query: 134 GNIYSPAAAPLAPSGADIPASYRNLDSRQQAMLFTDRVQFGERWEVLAGGRQVWLKEKTY 193 N+Y PA PLAP A +R + TD + + +L GG + +++ Y Sbjct: 419 SNLYQPA--PLAPVTAARGEPLPQSQTRLSGLALTDTLSIADGRLLLTGGLR---RQQVY 473 Query: 194 AATGATTRDTDRNQFLPQLALIYKPQVNMSLYGSYAETLSMGTAAAFWQTNYPTILPPSV 253 + + +T +++ + P L ++ KP N+SLY +Y LS G A N P Sbjct: 474 SNSFSTRSSSNKIGYSPVLGVVVKPLENLSLYANYTAGLSAGGFAGAQYANAGQAFAPYK 533 Query: 254 ARQLETGIKYDWNKDLSFTAAIFRMKKAYEYPQPDGAGGFKYVQQGKQTHTGLELGAAGN 313 +Q E+G+K DW + ++ TAA+F++++A G+ F Q G+ GLEL A G Sbjct: 534 TKQYESGVKLDWGR-VTTTAAVFQIERANSSTAVVGSQTFA-TQDGRARVRGLELSAYGE 591 Query: 314 VTKQLRLAASLAFIQARADGTGTPAYDDKQAVNVPRLRGALYADYALAALPGLNLQGSWL 373 + LRL AS F + R T D K+ VP+ L AD+ L GL L + Sbjct: 592 AQRGLRLMASGTFFETRQSHTVGGTNDGKEIGAVPKHNFNLGADWDTPWLQGLALNTRII 651 Query: 374 YSGSKSATRDGSATVPSYHIFNTGLRYQTRMGGHPTTVRLTVDNVFDKRYWKDTGESLGD 433 +G+ + + VP + + G RY +R+GG T R ++N+FDK YW + + Sbjct: 652 NTGASWYSDANTVKVPGWTRMDLGARYTSRLGGQTITYRANLENLFDKHYWI-----MQN 706 Query: 434 SYLHLGAPRLARLSVQYDF 452 Y GA R LS Q DF Sbjct: 707 GYAGYGAGRTLVLSAQVDF 725 >gi|300311660|ref|YP_003775752.1| TonB-dependent receptor protein [Herbaspirillum seropedicae SmR1] Length = 705 Score = 83.2 bits (204), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 94/359 (26%), Positives = 149/359 (41%), Gaps = 20/359 (5%) Query: 97 VQGKFNMGALRHGLTLGLTMSRRVIDKSDGISIPVGTGNIYSPAAAPLAPSGADIPASYR 156 ++G+ GA H + GL SR + D+ + NI +P P+ + + Sbjct: 360 LEGRLKWGATEHTVLFGLDWSR-MYDREVRYRGAAPSLNIDNPVYGVTIPAATTL---FG 415 Query: 157 NLDSR--QQAMLFTDRVQFGERWEVLAGGRQVWLKEKTYAATGATTRDTDRNQFLPQLAL 214 NL+ Q + D+++F +R + GGR +K++T TT +D + ++ L Sbjct: 416 NLEENMNQTGLYAQDQIKFDQRLTLTLGGRWDKVKDETRNYFNRTTTRSDDSALSGRIGL 475 Query: 215 IYKPQVNMSLYGSYAETLSMGTAAAFWQTNYPTILPPSVARQLETGIKYD-WNKDLSFTA 273 Y +++ Y SYA + T + F P P+ ARQ E GIKY + FTA Sbjct: 476 SYLVTRDIAPYVSYATSFLPQTGSDF--NGKP--FDPTKARQAEVGIKYQPADGKTLFTA 531 Query: 274 AIFRMKKAYEYPQPDGAGGFKYVQQGKQTHTGLELGAAGNVTKQLRLAASLAFIQARADG 333 A+F + K PD A V G+ GLEL A G + K L L+AS + + Sbjct: 532 ALFELTKT-NVQTPDPAHTNFNVLSGEIKSRGLELEAKGEIVKGLNLSASYTYTDVKNTQ 590 Query: 334 TGTPAYDDKQAVNVPRLRGALYADYALAALPGLNLQGSWL--YSGSKSATRDGSATVPSY 391 T + KQ + VPR +++ADY + P Y+GS A + Sbjct: 591 NNTASLLGKQPILVPRQAASVWADYTVQGGPLAGAGIGGGARYTGSNYADAANTVKNGGV 650 Query: 392 HIFNTGLRYQTRMGGHPTTVRLTVDNVFDKRYWKDTGESLGDSYLHLGAPRLARLSVQY 450 +F+ Y + L + N+ +K Y E + A R A LS +Y Sbjct: 651 TVFDAVFHYNLNRWRYA----LNISNITNKEYTSCLAEPTTTCF--WAAERTAILSARY 703 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,396,240 Number of extensions: 56515 Number of successful extensions: 166 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 158 Number of HSP's successfully gapped: 14 Length of query: 452 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 364 Effective length of database: 1,203,190 Effective search space: 437961160 Effective search space used: 437961160 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)