BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0241.p (227 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310322|ref|YP_003774414.1| LysR family transcription regul... 316 9e-88 gi|300312785|ref|YP_003776877.1| transcription regulator protein... 100 1e-22 gi|300310059|ref|YP_003774151.1| LysR family transcription regul... 80 2e-16 gi|300312373|ref|YP_003776465.1| LysR family transcription regul... 77 8e-16 gi|300312300|ref|YP_003776392.1| LysR family transcription regul... 74 1e-14 >gi|300310322|ref|YP_003774414.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 288 Score = 316 bits (810), Expect = 9e-88, Method: Compositional matrix adjust. Identities = 145/211 (68%), Positives = 180/211 (85%) Query: 12 DQLQRQMQGIDERLCGVVRIAATDTMAKHVLIPAMQQLAADHPAIRVVLTTSTQVSNLTR 71 +QLQRQMQGIDERLCGVVR+AATD A+H LIPA+ +L +HP IRVVL+TSTQ++NLT+ Sbjct: 76 EQLQRQMQGIDERLCGVVRLAATDVTARHFLIPALHRLRQEHPHIRVVLSTSTQITNLTK 135 Query: 72 READLAVRSLRPTSPDLISRHLKRLALGLYASRSYIKKHGLPQPGTALAGHDVVIYQRAI 131 READ+AVR+LRP +PDLI+RHLKRL +YASRSY+KK GLP+PGT L GHDVVIYQR++ Sbjct: 136 READIAVRTLRPDAPDLIARHLKRLHTAIYASRSYVKKRGLPEPGTGLKGHDVVIYQRSV 195 Query: 132 YASQIDTICGESTANARVAMEVSSGLMLLEAVAAGLGIGELPTYMADTVPQLVRLWPERA 191 ++ + ICGES NA+VAMEVS+GL++ + + AG G+GELPT++ D LVR+WP+R Sbjct: 196 SPAKSEQICGESIINAQVAMEVSTGLVMYDCLQAGFGLGELPTHVGDKASGLVRVWPQRE 255 Query: 192 EPYDMWLVLHSDLNRTARVRAVADAIVAAYD 222 PYDMWLVLHSDLNRTARVRAVADAI+AAY+ Sbjct: 256 TPYDMWLVLHSDLNRTARVRAVADAIIAAYE 286 >gi|300312785|ref|YP_003776877.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 297 Score = 100 bits (248), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 70/204 (34%), Positives = 106/204 (51%), Gaps = 5/204 (2%) Query: 19 QGIDERLCGVVRIAATDTMAKHVLIPAMQQLAADHPAIRVVLTTSTQVSNLTRREADLAV 78 QG + R G VR+ A++ ++ VL P + L HP +RV L S + +L +READ+AV Sbjct: 90 QGKEVR--GTVRLTASEVISVEVLPPILAALRHRHPELRVELVLSNKADDLLQREADIAV 147 Query: 79 RSLRPTSPDLISRHLKRLALGLYASRSYIKKHGLPQPGTALAGHDVVIY-QRAIYASQID 137 R RP L+++ L + LGL+A +SY+ HG P+ ALA H ++ + Q + Y + Sbjct: 148 RMFRPAQDALVAKRLGSIELGLHAHQSYLTVHGAPKSVDALADHTLIGFDQESAYVRNVL 207 Query: 138 TICGESTANARVAMEVSSGLMLLEAVAAGLGIGELPTYMADTVPQLVRLWPER-AEPYDM 196 + A +A+ S L L A+ AG GIG +A LVRL P + A + Sbjct: 208 KQI-PWLSRAALALRTDSDLAQLAAIRAGFGIGICQVALAARDVALVRLLPRQFALKMET 266 Query: 197 WLVLHSDLNRTARVRAVADAIVAA 220 W+ +H DL + R A+VA Sbjct: 267 WVAMHEDLRASPRCAVTFAALVAG 290 >gi|300310059|ref|YP_003774151.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 283 Score = 79.7 bits (195), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 71/234 (30%), Positives = 108/234 (46%), Gaps = 27/234 (11%) Query: 1 VSAVEKMETAADQLQRQMQGIDERLCGVVRIAATDTMAKHVLIPAMQQLAADHPAIRVVL 60 ++A M+ QL R + G D R+ G +R+A TD M L P + L PA+ + L Sbjct: 67 LAAARAMQDQTRQLARTLAGEDLRMEGKLRLACTDAMGTLFLAPRLPSLLQQWPALDIEL 126 Query: 61 TTSTQVSNLTRREADLAVRSLRPTSPDLISRHLK--RLALGLYASRSYIKKHGLPQPGTA 118 Q +L RREADLA+R RP +LI+R L R L A + +H Q G+A Sbjct: 127 AMGHQTLSLARREADLALRFARPQDGELIARRLGVVRFYLCGTADQVAAWRH---QRGSA 183 Query: 119 -LAGHDVVIYQRAIYASQIDTICGESTANARVAMEVSSGLMLLEAVAAGLGIGELPTYM- 176 + G+D + + + + N V + +S + A AG+G+ LP+Y+ Sbjct: 184 PVIGYDDSVPD----IPETRWLAAHADGNP-VRLRSASLVAQCMAARAGVGLALLPSYLL 238 Query: 177 ------ADTVPQLVRLWPERAEPYDMWLVLHSDLNRTARVRAVADAIVAAYDRL 224 ++ PQLVR ++W V H DL R+RAV + + A L Sbjct: 239 APELQTVESTPQLVR---------ELWAVFHRDLKALPRLRAVLEWLQACCGEL 283 >gi|300312373|ref|YP_003776465.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 305 Score = 77.4 bits (189), Expect = 8e-16, Method: Compositional matrix adjust. Identities = 60/214 (28%), Positives = 96/214 (44%), Gaps = 1/214 (0%) Query: 1 VSAVEKMETAADQLQRQMQGIDERLCGVVRIAATDTMAKHVLIPAMQQLAADHPAIRVVL 60 V E++E+ ++ G + +L G VR+ AT+ VL P + A +P I V + Sbjct: 66 VEYAEQVESTLYAATEELGGHNSKLSGQVRLGATEGFGTFVLAPHIAHFCARNPHITVDM 125 Query: 61 TTSTQVSNLTRREADLAVRSLRPTSPDLISRHLKRLALGLYASRSYIKKHGLPQPGTALA 120 + NL++ EAD++V RP S + + L L LYASR Y+ +H LA Sbjct: 126 LPMPRFVNLSKHEADISVSIERPLSGNYVVTKLSDYVLKLYASRDYLAQHPPIHTMDDLA 185 Query: 121 GHDVVIYQRAIYASQIDTICGESTANARVAMEVSSGLMLLEAVAAGLGIGELPTYMADTV 180 H + Y + S+ +A A +S + A AG + LP ++A Sbjct: 186 QHHFIGYIDDLVFSEELRYKDTVAPDAFRAFRSTSVVAQYTAACAGRALAILPCFLAQQS 245 Query: 181 PQLVRLWPER-AEPYDMWLVLHSDLNRTARVRAV 213 +L + P++ A WLV S+ ARV A+ Sbjct: 246 EELAPVLPQQIAITRSFWLVASSEQRHVARVSAL 279 >gi|300312300|ref|YP_003776392.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 305 Score = 73.9 bits (180), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 60/222 (27%), Positives = 109/222 (49%), Gaps = 20/222 (9%) Query: 6 KMETAADQLQRQMQGI------DERLCGVVRIAATDTMAKHVLIPAMQQLAADHPAIRVV 59 ++ A+QL+ +M + + L G VR++A + L+P + +P I + Sbjct: 85 ELSKQAEQLEVEMHSLWRAALANTTLSGKVRVSAPPILMSDFLVPRLPAFQEKYPDIELE 144 Query: 60 LTTSTQVSNLTRREADLAVRSLRPTSPDLISRHLKRLALGLYASRSYIKKHGLPQPGTAL 119 + + +LTR EAD+A+R P SP L++R L ++ +Y +++++ P+ + Sbjct: 145 VIGEARGVDLTRGEADIALRMGEPVSPLLVARMLCNVSYSVYGIPAWLER---PESERSF 201 Query: 120 AGHD--VVIYQRAIYASQIDTICGESTANARVAMEVSSGLMLLEAVAAGLGIGELPTYMA 177 G D Q+A SQ+D R A+ + + A +G+G+ LP +M Sbjct: 202 IGLDQESPCRQKAWLESQVD--------QRRYALRTNDIRAMCHAAMSGIGLALLPRFMV 253 Query: 178 DTVPQLVRL-WPERAEPYDMWLVLHSDLNRTARVRAVADAIV 218 +LV + PE P ++LV+HSD+ R R+RA AD ++ Sbjct: 254 AEDTRLVAIPMPETPPPCPLYLVMHSDVRRAPRIRATADFLI 295 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.131 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 627,335 Number of extensions: 23488 Number of successful extensions: 168 Number of sequences better than 1.0e-08: 12 Number of HSP's gapped: 165 Number of HSP's successfully gapped: 12 Length of query: 227 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 145 Effective length of database: 1,231,600 Effective search space: 178582000 Effective search space used: 178582000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)