BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0239.p (463 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310323|ref|YP_003774415.1| methylenomycin A resistance pro... 607 e-175 gi|300312966|ref|YP_003777058.1| major facilitator superfamily p... 150 1e-37 gi|300311185|ref|YP_003775277.1| major facilitator superfamily p... 135 9e-33 gi|300312017|ref|YP_003776109.1| multidrug resistance B (translo... 122 5e-29 gi|300312566|ref|YP_003776658.1| multidrug resistance protein pr... 119 6e-28 >gi|300310323|ref|YP_003774415.1| methylenomycin A resistance protein [Herbaspirillum seropedicae SmR1] Length = 478 Score = 607 bits (1566), Expect = e-175, Method: Compositional matrix adjust. Identities = 335/441 (75%), Positives = 372/441 (84%) Query: 4 QVGLLPLVTLAIGFVMAMLDVTVVNVGLSSIEKSLATPLSMLVWIVDGYTLSFAAMLLVG 63 + G+LPLV LAIGFVMAMLDVTVVNVGLS+I SL PLS LVWIVDGYTL+FAAMLLVG Sbjct: 26 RAGMLPLVALAIGFVMAMLDVTVVNVGLSNIAGSLDAPLSTLVWIVDGYTLTFAAMLLVG 85 Query: 64 GALADRYGAKNIYLLGLVLFVVASLLCGAAPNGGMLVAARLLQGLGAAFFMPSSLSLLTH 123 GALADR+GA+ +YL GL LFV+ASLLCGAAPNG L+ ARLLQGLGAAFFMPSSLSLLTH Sbjct: 86 GALADRFGARRLYLTGLFLFVLASLLCGAAPNGPFLIVARLLQGLGAAFFMPSSLSLLTH 145 Query: 124 VYEDDRVRARMLGVWSATVGGAAAVGPLVGGILIHSFGWRSVFLINVPVGLLGLYMAVKM 183 VYEDDRVRARMLGVWSATVG AAAVGPLVGG+LIH GWRSVFLINVPVGL+GL MA K+ Sbjct: 146 VYEDDRVRARMLGVWSATVGLAAAVGPLVGGVLIHWLGWRSVFLINVPVGLVGLVMARKL 205 Query: 184 IPSVERHVRALTIASHFLGVLMLAALSFVLIEGPVYGWTSLPIATAVVVTMLAFGTLVQR 243 IP V H RAL ++SH GV+MLA LSFVLI+GPVYGWTS I + V + A L++ Sbjct: 206 IPQVGGHARALNLSSHLAGVVMLAGLSFVLIQGPVYGWTSPRILAGLAVALSAGAALIRH 265 Query: 244 ERSGSAPLLPRALFATPQFAAANGIGFLINFGVYGQFFFLALFLQQARGADALQTGLQML 303 E G APLLP+ALFATPQFAAANGIGFLINFGVYG F+LALFLQQ RGADALQTGLQ++ Sbjct: 266 ELRGIAPLLPKALFATPQFAAANGIGFLINFGVYGNLFYLALFLQQGRGADALQTGLQLV 325 Query: 304 PMMAVIFIGNLNSGRMTARFGPRLPLRLGLTVGAVFSAVQIGLTPETPYWMLAVAGALAN 363 PMMAVIF GNL SGRM+A +GPR+PL LGL+VG VF+A+ +G +P TPYW+LA+ A AN Sbjct: 326 PMMAVIFFGNLMSGRMSAHWGPRVPLLLGLSVGTVFTALAMGFSPATPYWLLALVCASAN 385 Query: 364 LGISTAIPAMTTAVMQVAGKGHANSAAAALNANRQIGALVGVALMGAILHSFGNWITRIV 423 LGISTAIPAMTT VMQVAGK HANSAAAALNANRQIGALVGVALMGAILHS W R Sbjct: 386 LGISTAIPAMTTVVMQVAGKAHANSAAAALNANRQIGALVGVALMGAILHSVTGWNLRNP 445 Query: 424 LAYGVVAIVYALALVLVLRYV 444 LA+GV+A YA AL LV R+V Sbjct: 446 LAFGVMAAGYASALWLVWRFV 466 >gi|300312966|ref|YP_003777058.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 544 Score = 150 bits (380), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 106/299 (35%), Positives = 152/299 (50%), Gaps = 7/299 (2%) Query: 10 LVTLAIGFVMAMLDVTVVNVGLSSIEKSLATPLSMLVWIVDGYTLSFAAMLLVGGALADR 69 L+T + G M +LD VV + L ++ + L S + W+V Y L FAA+LL GA ADR Sbjct: 38 LLTGSFGCAMTVLDTNVVGIILPTVARDLGASFSQIEWVVSSYVLCFAALLLPAGATADR 97 Query: 70 YGAKNIYLLGLVLFVVASLLCGAAPNGGMLVAARLLQGLGAAFFMPSSLSLLTHVYEDDR 129 YG + + L G+ LF + SL C AP+ L AR LQG+GAAF + +L+++ H + D+ Sbjct: 98 YGRRRVLLGGIALFALTSLACALAPSAQALYGARALQGVGAAFLLAPALAVIGHAFHDED 157 Query: 130 VRARMLGVWSATVGGAAAVGPLVGGILIHSFGWRSVFLINVPVGLLGLYMAVKMIPS--- 186 R R +W +G + PL+GG++ GWR F +N+P LL + +P Sbjct: 158 QRERAWALWGGIMGLTMVLAPLIGGVVNTLLGWRWAFAVNLPACLLLAVAVWRHVPESRN 217 Query: 187 -VERHVRALTIASHFLGVLMLAALSFVLIEGPVYGWTSLPIATAVVVTMLAFGTLVQRER 245 + R + L I S V + L++ LI GP +GWTS+ + L + +R Sbjct: 218 PLPRPLDPLGILSSSSAVFL---LTWALITGPEHGWTSVACVARGLGGALLLVLFIAWQR 274 Query: 246 SGSAPLLPRALFATPQFAAANGIGFLINFGVYGQFFFLALFLQQARGADALQTGLQMLP 304 + P+L +LF F AA F L LFLQ ARG A QTGL MLP Sbjct: 275 RAAHPMLELSLFRHAGFVAAVAAMFAYAASAQVMASLLPLFLQNARGLSAWQTGLAMLP 333 >gi|300311185|ref|YP_003775277.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 532 Score = 135 bits (339), Expect = 9e-33, Method: Compositional matrix adjust. Identities = 119/412 (28%), Positives = 195/412 (47%), Gaps = 13/412 (3%) Query: 10 LVTLAIGFVMAM--LDVTVVNVGLSSIEKSLATPLSMLVWIVDGYTLSFAAMLLVGGALA 67 L TL I FV+ + LD T+V L +I L + W+ Y L+ + + G L Sbjct: 22 LATLGICFVIMLVALDQTIVGTALPTIVAELKG-FELYAWVATAYLLTSVITVPIFGRLG 80 Query: 68 DRYGAKNIYLLGLVLFVVASLLCGAAPNGGMLVAARLLQGLGAAFFMPSSLSLLTHVYED 127 D +G K + +++FV AS LCG A + LV AR LQG+G + + + + ++ D Sbjct: 81 DYFGRKPFVIASIIVFVGASALCGMANSMLFLVLARGLQGIGGGMLVGTCFASVADLFPD 140 Query: 128 DRVRARMLGVWSATVGGAAAVGPLVGGILIHSFGWRSVFLINVPVGLLGLYMAVKMIPSV 187 RVR R + S + G A AVGP +GG L GWR VF N+P+G++ L+ + +P + Sbjct: 141 PRVRLRWQIILSVSFGIATAVGPSLGGFLTQGLGWRWVFYCNLPIGVISLFFVWRFVPHI 200 Query: 188 ERHVRALTIASHFLGVLMLAALSFVLIEG----PVYGWTSLPIATAVVVTMLAFGTLVQR 243 A + + G L+++ L P G TS + + V++ AF L + Sbjct: 201 RHMQHAGKMRLDWPGALLISLSLGSLQLLVELLPKRGVTS-GMLVLLAVSVAAFYALWKW 259 Query: 244 ERSGSAPLLPRALFATPQFAAANGIGFLINFGVYGQFFFLALFLQQARGADALQTGLQML 303 E+ + P+LP + A+ + L F ++ + L Q G + GL + Sbjct: 260 EQRAAQPILPFEMLRDRALASLFLLSVLAGFAMFSMLMYAPLLFQGGFGLSPREAGLLIT 319 Query: 304 PMMAVIFIGNLNSGRMTARF-GPRLPLRLGLTVGAVFSAVQIGLTPET--PYWMLAVAGA 360 P++A I + ++ +GR+ R P L + LG + A S + I L ++LAV A Sbjct: 320 PLVACITVASIVNGRIITRIPNPNLMMNLGFALIAA-SCLGIVLCDRVTGKGFLLAVMLA 378 Query: 361 LANLGISTAIPAMTTAVMQVAGKGHANSAAAALNANRQIGALVGVALMGAIL 412 G+ +P +T Q A + H A A L + R IG ++G A+ G ++ Sbjct: 379 -GGFGLGLVLPNLTVFAQQAAAREHLGIATALLQSLRMIGGMLGTAITGTLV 429 >gi|300312017|ref|YP_003776109.1| multidrug resistance B (translocase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 524 Score = 122 bits (307), Expect = 5e-29, Method: Compositional matrix adjust. Identities = 92/299 (30%), Positives = 152/299 (50%), Gaps = 6/299 (2%) Query: 11 VTLAIGFVMAMLDVTVVNVGLSSIEKSLATPLSMLVWIVDGYTLSFAAMLLVGGALADRY 70 +++++ M +LD ++ NV + +I +L + W++ + + A + + G L R Sbjct: 27 LSVSLAVFMNVLDSSIANVAIPTISGNLGVSVDEGTWVITSFAAANAISIPLTGWLTQRL 86 Query: 71 GAKNIYLLGLVLFVVASLLCGAAPNGGMLVAARLLQGLGAAFFMPSSLSLLTHVYEDDRV 130 GA +++ ++LFV+AS LCG AP +L+AAR+LQGL A +P S SLL Y + Sbjct: 87 GAVRLFVTSVLLFVLASWLCGLAPTLPILLAARVLQGLVAGPMVPLSQSLLLGAYPKSK- 145 Query: 131 RARMLGVWSATVGGAAAVGPLVGGILIHSFGWRSVFLINVPVGLLGLYMAVKMIPSVERH 190 + L +W T A GP +GG + S+ W +F IN+PVGL+ + + + S E Sbjct: 146 SSFALALWGMTAVVAPVAGPALGGWITDSYTWPWIFYINIPVGLIAAGVTLAIYRSRETP 205 Query: 191 VRALTIASHFLGVLM--LAALSFVLIEGPVYGWTSLP-IATAVVVTMLAFGTLVQRERSG 247 L I L +L+ +AA +L +G W + P I T ++ ++AF V E + Sbjct: 206 THKLPIDKIGLALLVTWVAAFQIMLDKGKDLDWFASPTIVTLGIIALIAFAYFVVWELNN 265 Query: 248 SAPLLPRALFATPQFAAANGIGFLINFGV-YGQFFFLALFLQQARGADALQTGLQMLPM 305 P++ LF FA I I +GV +G L +LQQ G ++ +GL P+ Sbjct: 266 DHPVVDLRLFGRRNFAGGT-IAISIGYGVFFGNLVILPQWLQQYLGYRSIDSGLSTAPI 323 >gi|300312566|ref|YP_003776658.1| multidrug resistance protein protein [Herbaspirillum seropedicae SmR1] Length = 525 Score = 119 bits (297), Expect = 6e-28, Method: Compositional matrix adjust. Identities = 90/292 (30%), Positives = 147/292 (50%), Gaps = 4/292 (1%) Query: 11 VTLAIGFVMAMLDVTVVNVGLSSIEKSLATPLSMLVWIVDGYTLSFAAMLLVGGALADRY 70 + LA +A+LD T+ NV +S+I +L S +++ Y ++ A + + G LA+R+ Sbjct: 36 ILLAAANFIAVLDTTIANVSVSTIAGALGASTSQGTYVITSYAVAEAITVPLTGWLANRF 95 Query: 71 GAKNIYLLGLVLFVVASLLCGAAPNGGMLVAARLLQGLGAAFFMPSSLSLLTHVYEDDRV 130 G +++L +V+F + S LCG A + GML+A R+LQGL MP S +LL ++ ++ Sbjct: 96 GTVRVFILSMVMFGLFSALCGMANSLGMLIAFRVLQGLAGGPLMPLSQTLLLRIFPKEKA 155 Query: 131 RARMLGVWSATVGGAAAVGPLVGGILIHSFGWRSVFLINVPVGLLGLYMAVKMIPSVERH 190 A +G+W+ T A GP++GG+L + W +F INVPV L+ Y+ +M+ E Sbjct: 156 PAA-VGLWAMTTLVAPIAGPILGGVLCDQYSWPYIFWINVPVALICGYIGWRMLKRYETK 214 Query: 191 VRALTIAS--HFLGVLMLAALSFVLIEGPVYGW-TSLPIATAVVVTMLAFGTLVQRERSG 247 + L I L V+ +AAL +L EG W S I V ++ F + E + Sbjct: 215 MSKLPIDRVGLVLMVVWVAALQLMLDEGKEKDWFASTEIVLLAVTAVVFFIAFLIWELTE 274 Query: 248 SAPLLPRALFATPQFAAANGIGFLINFGVYGQFFFLALFLQQARGADALQTG 299 P++ +F F A+ L +G L+LQ G A +G Sbjct: 275 RNPIVDLRVFRHRGFTASVTTICLAFGAFFGSVVLTPLWLQGYMGYTATWSG 326 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.140 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,120,126 Number of extensions: 41008 Number of successful extensions: 376 Number of sequences better than 1.0e-08: 26 Number of HSP's gapped: 355 Number of HSP's successfully gapped: 26 Length of query: 463 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 374 Effective length of database: 1,198,455 Effective search space: 448222170 Effective search space used: 448222170 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)