BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0229.p (265 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313109|ref|YP_003777201.1| short-chain alcohol dehydrogena... 357 e-100 gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herb... 140 1e-34 gi|300313172|ref|YP_003777264.1| short-chain alcohol dehydrogena... 132 2e-32 gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independe... 129 2e-31 gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein... 125 3e-30 >gi|300313109|ref|YP_003777201.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 266 Score = 357 bits (917), Expect = e-100, Method: Compositional matrix adjust. Identities = 166/264 (62%), Positives = 207/264 (78%) Query: 1 MSERMKGKVAIVIGAGSSGPGWGNGKAAAVLYAREGARIFAVDFRIEAAQETAAIIRSEG 60 M + M+GK IV GAGS G GWGNGKAAAVLYAREG R+ AVD IEAA +T IIRSEG Sbjct: 1 MDKLMQGKAIIVTGAGSVGEGWGNGKAAAVLYAREGGRVLAVDRSIEAALQTQDIIRSEG 60 Query: 61 GECTAFRADVASSDDIREMAAACMATYGQIDVLHNNVGIVETGGPVETSEESWDRVIAVN 120 G C ADV+ + +++ +A A M +G++DVLHNNVGI + GGPVETSEESW+R++AVN Sbjct: 61 GVCEVLAADVSRNTEVKAIAEAAMDHFGRVDVLHNNVGIADVGGPVETSEESWNRLVAVN 120 Query: 121 QTSIFLTCKHVLPYMAARNAGAIVNIGSIAGLRWIGFPYLAYSAAKAALISMTSNIAVEY 180 QTS+FLT KHVLP M + GAIVNI S+A LRWIGFPYL Y+A KAA+++ T N+A++Y Sbjct: 121 QTSLFLTHKHVLPIMEKQRKGAIVNISSLAALRWIGFPYLGYTATKAAILAFTKNVAMQY 180 Query: 181 AAQGIRANCVLPGLMNTPLIREPLTKTYGGNVDSMIAQRDKISPTGKMGDAWDTAYAALF 240 A GIRANCVLPG+M+TP+IREPL +YGG+++ M A+R+ P G MGDAWD A+A+LF Sbjct: 181 APMGIRANCVLPGMMDTPMIREPLKASYGGDIEQMRAKRNAQCPMGFMGDAWDVAHASLF 240 Query: 241 LASDEARYVNGTSIIVDGGLTARC 264 LASD ARY+ G +IVDGGL+ RC Sbjct: 241 LASDHARYITGLDMIVDGGLSLRC 264 >gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 140 bits (353), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 97/262 (37%), Positives = 141/262 (53%), Gaps = 22/262 (8%) Query: 5 MKGKVAIVIGAGSSGPGWGNGKAAAVLYAREGARIFAVDFRIEAAQETAAIIRSEGGECT 64 ++GKVA++ GA + G G+A A+L+AR GA + R +A ++ AA+I ++GG + Sbjct: 4 LQGKVALITGASA-----GIGRATALLFARHGAALILTARRQDALEQVAALICAQGGRAS 58 Query: 65 AFRADVASSDDIREMAAACMATYGQIDVLHNNVGIVETGGPV-ETSEESWDRVIAVNQTS 123 DV + A + +G +D+ NN G V P+ E S + W + N TS Sbjct: 59 VVAGDVGEAATHERGVAMALDEFGGLDIAINNAGAVGACKPLAEISPQQWQDTLHANLTS 118 Query: 124 IFLTCKHVLPYMAARNAGAIVNIGSIAGLRWIGFPYL-AYSAAKAALISMTSNIAVEYAA 182 FL + +P M AR AGAIV S G G P + AY AAKA L+ + IA +YA Sbjct: 119 AFLGARCQIPAMLARGAGAIVFTSSFVG-SSAGLPGMAAYGAAKAGLMGLVKGIAADYAV 177 Query: 183 QGIRANCVLPGLMNTPLIREPLTKTYGGNVDSM--IAQRDKISPTGKMGDAWDTAYAALF 240 QGIRAN +LPG ++T + +P K + + +M IAQ ++I A AALF Sbjct: 178 QGIRANALLPGGVDTDMGGDPQQKQWAAGLHAMKRIAQPEEI------------AAAALF 225 Query: 241 LASDEARYVNGTSIIVDGGLTA 262 LAS A +V GT++ DGG +A Sbjct: 226 LASPMASFVTGTALYADGGNSA 247 >gi|300313172|ref|YP_003777264.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 254 Score = 132 bits (333), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 89/259 (34%), Positives = 135/259 (52%), Gaps = 17/259 (6%) Query: 4 RMKGKVAIVIGAGSSGPGWGNGKAAAVLYAREGARIFAVDFRIEAAQETAAIIRSEGGEC 63 R+ GK ++ A G G+A+ L+AREGAR+ A D E A I E Sbjct: 7 RLAGKTVLITAAAQ-----GIGRASTELFAREGARVIATDISKPHLDELAGIAGVE---- 57 Query: 64 TAFRADVASSDDIREMAAACMATYGQIDVLHNNVGIVETGGPVETSEESWDRVIAVNQTS 123 DV I+ A +A G IDVL N G V G +E +++WD +N + Sbjct: 58 -THLLDVTDDAAIK----ALVAKIGTIDVLFNCAGYVAAGNILECDDKAWDFSFNLNAKA 112 Query: 124 IFLTCKHVLPYMAARNAGAIVNIGSIAGLRWIGFPYLAYSAAKAALISMTSNIAVEYAAQ 183 +F T + VLP M A+ AG+IVNI S A AY A+KAA++ +T ++A ++ AQ Sbjct: 113 MFHTIRAVLPGMLAKKAGSIVNIASAASSVKGVANRFAYGASKAAVVGLTKSVAADFVAQ 172 Query: 184 GIRANCVLPGLMNTPLIREPLT---KTYGGNVDSMIAQRDKISPTGKMGDAWDTAYAALF 240 GIR N + PG + +P + + ++ K G + + + A P G++G A + A AL+ Sbjct: 173 GIRCNAICPGTIESPSLNQRISTQAKETGKSEEEVRAAFVGRQPMGRIGKAEEVAALALY 232 Query: 241 LASDEARYVNGTSIIVDGG 259 LASDE+ + G+ ++DGG Sbjct: 233 LASDESNFTTGSIHMIDGG 251 >gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independent alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 252 Score = 129 bits (325), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 96/266 (36%), Positives = 137/266 (51%), Gaps = 19/266 (7%) Query: 4 RMKGKVAIVIGAGSSGPGWGNGKAAAVLYAREGARIFAVDFRIEAAQETAAIIRSEGGEC 63 R++ KVAIV G GS G+ A YAREGA I D I++ GG Sbjct: 2 RLQDKVAIVTGGGSG-----FGEGIAKAYAREGAAIVVADIGEAGGLRVVEEIKAAGGRA 56 Query: 64 TAFRADVASSDDIREMAAACMATYGQIDVLHNNVGIVETGGP-VETSEESWDRVIAVNQT 122 +ADV+ D+ ++ A + +G++D++ NN G P +E E+ +DRV AVN Sbjct: 57 VFAKADVSKRADMDQLLATALEHFGKLDIVVNNAGTTHRNRPMLEVEEDEFDRVYAVNVK 116 Query: 123 SIFLTCKHVLPYMAARNAGAIVNIGSIAGLRWIGFPYLA-YSAAKAALISMTSNIAVEYA 181 SIFL+ K +PY GA +NI S AG+R P L Y+ +K A+I+ + ++A E Sbjct: 117 SIFLSAKTFVPYFRQVGGGAFINIASTAGIR--PRPGLTWYNGSKGAVITTSKSMAAELG 174 Query: 182 AQGIRANCVLPGLMNTPLIREPLTKTYGGNVDSMIAQRDKIS--PTGKMGDAWDTAYAAL 239 IR NCV P + T L+ E + G D+ ++ ++ P G+ D A A L Sbjct: 175 PDKIRVNCVNPVISATGLLSE-----FMGVPDTPENRKKFVATIPMGRFSTPEDIANACL 229 Query: 240 FLASDEARYVNGTSIIVDGGLTARCV 265 +L SDEA +V G I VDGG RCV Sbjct: 230 YLGSDEAEFVTGVCIEVDGG---RCV 252 >gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 125 bits (315), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 87/260 (33%), Positives = 140/260 (53%), Gaps = 20/260 (7%) Query: 5 MKGKVAIVIG-AGSSGPGWGNGKAAAVLYAREGARIFAVDFRIEAAQETAAIIRSEGGEC 63 +K KVAI+ G AG +G G+ + A L A +GARI +D +E A+ A R G + Sbjct: 3 LKDKVAIITGGAGQNGLGF----STARLMAAQGARIVILD--LERAEPAGAAARL-GAQH 55 Query: 64 TAFRADVASSDDIREMAAACMATYGQIDVLHNNVGIVETGGPVETSEESWDRVIAVNQTS 123 ADV AAA + +G+ID+L NN G+ + +E + + +DR++ V+ Sbjct: 56 LGLVADVTDKAACEAAAAAVLKQFGRIDILVNNAGVTQPAKLLEITGKDYDRILDVSLRG 115 Query: 124 IFLTCKHVLPYMAARNAGAIVNIGSIAGLR---WIGFPYLAYSAAKAALISMTSNIAVEY 180 + VLP M A+ +G+IV I S++ R +G P+ YSAAKA ++ + +A E+ Sbjct: 116 TLYMSQAVLPAMQAQQSGSIVCISSVSAQRGGGILGGPH--YSAAKAGVLGLARAMAREF 173 Query: 181 AAQGIRANCVLPGLMNTPLIREPLTKTYGGNVDSMIAQRDKISPTGKMGDAWDTAYAALF 240 QGIR NC+ PGL++T + ++ L+ + I P G++G D +F Sbjct: 174 GPQGIRVNCITPGLISTDINKDILSAEKKAEIAQTI-------PLGRLGGPDDVGGVCVF 226 Query: 241 LASDEARYVNGTSIIVDGGL 260 LASD ++Y G ++ V+GG+ Sbjct: 227 LASDLSQYCTGVTLDVNGGM 246 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.133 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 756,554 Number of extensions: 29965 Number of successful extensions: 222 Number of sequences better than 1.0e-08: 47 Number of HSP's gapped: 141 Number of HSP's successfully gapped: 47 Length of query: 265 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 181 Effective length of database: 1,222,130 Effective search space: 221205530 Effective search space used: 221205530 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)