BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0224.p (432 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313915|ref|YP_003778007.1| transmembrane arabinose efflux ... 309 3e-85 gi|300312509|ref|YP_003776601.1| arabinose efflux permease [Herb... 142 5e-35 gi|300312245|ref|YP_003776337.1| transmembrane efflux transmembr... 139 3e-34 gi|300310405|ref|YP_003774497.1| arabinose efflux permease [Herb... 78 1e-15 gi|300312017|ref|YP_003776109.1| multidrug resistance B (translo... 55 9e-09 >gi|300313915|ref|YP_003778007.1| transmembrane arabinose efflux transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 403 Score = 309 bits (791), Expect = 3e-85, Method: Compositional matrix adjust. Identities = 185/373 (49%), Positives = 240/373 (64%), Gaps = 9/373 (2%) Query: 25 MNTSSLPASAPAASNAPLY--------WMALGTFAVGAEGFMIAGILPTVAKDLGVSVAV 76 MN L SAP+A +P ++ALGTFA+G EGFMIA +LP +A D G+SV + Sbjct: 1 MNPPPLGPSAPSACASPTQAGSLLTAGFLALGTFAIGTEGFMIAPLLPRMAADFGMSVPM 60 Query: 77 AGQLVVAFTAAYAISSPILTALTGALSRRGLLIGAMTAFTLANLLAWQAGGYWSLMTARI 136 LVV FT A+SSPI T L+ A++RR +LI AMTAFT ANLLA + + L+ AR+ Sbjct: 61 VASLVVIFTLVLALSSPISTVLSAAMNRRRVLIAAMTAFTAANLLAACSSSFTGLLVARL 120 Query: 137 LLAFAAGLYVPNALALAGALVAPEKRGSALALVSGGITVAVALAAPLGTVIADSLSWRMT 196 L+A AAGLY PNA ALAGA+VAP +RG ALA+VSGG+T AL PLG V+ + WR T Sbjct: 121 LMAVAAGLYTPNASALAGAMVAPGQRGRALAIVSGGMTSPSALGLPLGAVVGHAFGWRAT 180 Query: 197 FAGVALLAAIATLGLMLGLPRGIGNNLPVASLRERVLAARDPAVLRALLTTLLWATGAYT 256 F V+ + A+A G++LG+PR G +L VA LRER+ P V R L TLLW+ GAY Sbjct: 181 FLAVSAMGAVALAGILLGVPRQAGAHLHVAGLRERMAVIGQPQVQRLLAVTLLWSVGAYA 240 Query: 257 MYTYFALYLNQVTGIHGTHVGYVLFAWGISAAIGVMAGGKIADRVGFRRVTGPALMFLIA 316 Y Y A YL+ V + + + WG +AA+GV GG + DR G RV +L+ L+ Sbjct: 241 AYPYIAPYLSHVLSFGESRISLTVSLWGAAAAVGVSVGGTLNDRYGSARVVRVSLLLLML 300 Query: 317 AFLTLTAIAYLLPASLAVLPVFLSVIVWGISGWAFYPAQQTNLIGIAGIKLAPIAMSLNA 376 +F TL A A LP LA+ PV L+V+VWG S WAF+PAQ LIG AP+A+SLN Sbjct: 301 SFWTLAA-ATALPPPLALGPVLLAVVVWGFSVWAFFPAQMARLIGAGAPAQAPVALSLNT 359 Query: 377 SFMYLGFSIGASA 389 S +YLGFS+G++A Sbjct: 360 STLYLGFSLGSAA 372 >gi|300312509|ref|YP_003776601.1| arabinose efflux permease [Herbaspirillum seropedicae SmR1] Length = 385 Score = 142 bits (358), Expect = 5e-35, Method: Compositional matrix adjust. Identities = 117/350 (33%), Positives = 171/350 (48%), Gaps = 12/350 (3%) Query: 39 NAPLYWMALGTFAVGAEGFMIAGILPTVAKDLGVSVAVAGQLVVAFTAAYAISSPILTAL 98 N PL+ +A+ F +G F+I G+LP VA+DLGV+ AG LV + A +PIL L Sbjct: 2 NWPLFALAVAAFGIGTTEFVIMGLLPDVARDLGVTAPAAGMLVSGYALGVAAGAPILAIL 61 Query: 99 TGALSRRGLLIGAMTAFTLANLLAWQAGGYWSLMTARILLAFAAGLYVPNALALAGALVA 158 T R+ L+G M F L N+L A Y LM AR++ AF + +A LVA Sbjct: 62 TAKWPRKQALVGLMCLFILGNVLCALAPNYVFLMVARVVTAFCHAAFFGIGSVVAAELVA 121 Query: 159 PEKRGSALALVSGGITVAVALAAPLGTVIADSLSWRMTFAGVALLAAIATLGLMLGLPRG 218 P+KR A+AL+ G+TVA L PLGT + WR TF V + +A + L LP+ Sbjct: 122 PQKRAQAVALMFTGLTVANILGVPLGTAFGHAAGWRSTFGAVTAIGVLAAIMLWTMLPKK 181 Query: 219 IGNNLPVASLRERVLAARDPAVLRALLTTLLWATGAYTMYTYFALYLNQVTGIHGTHVGY 278 I + ++ + + D V +LLT+ L + +++ TY A L VTG + Sbjct: 182 I--PMQAGNIFQEFRSIIDTRVQLSLLTSTLASISMFSVITYIAYILLDVTGFTTQQESW 239 Query: 279 VLFAWGISAAIGVMAGGKIADRVGFRRVTGPALMFLIAAFLTLTAIAYLLPASLAVLPVF 338 VL +G +G + GGK+AD + G + IA L L +I S P Sbjct: 240 VLLLFGAGITVGGLVGGKLADWKLMPVMCG--VFLCIAVVLGLFSI-----TSHYKWPTL 292 Query: 339 LSVIVWGISGWAFYPAQQTNLIGIAGIKLAP-IAMSLNASFMYLGFSIGA 387 ++ WG +A P Q ++ A + AP +A +LN LG + GA Sbjct: 293 ATMFAWGAFAFAVVPGAQIRVLDTA--RGAPNLASTLNQGAFNLGNATGA 340 >gi|300312245|ref|YP_003776337.1| transmembrane efflux transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 395 Score = 139 bits (351), Expect = 3e-34, Method: Compositional matrix adjust. Identities = 95/259 (36%), Positives = 140/259 (54%), Gaps = 2/259 (0%) Query: 42 LYWMALGTFAVGAEGFMIAGILPTVAKDLGVSVAVAGQLVVAFTAAYAISSPILTALTGA 101 L+ + + FA+G F+I G+LP VA +LGVS++ AG LV + + +P+LTA TG Sbjct: 5 LWALTISAFAIGTTEFVIMGLLPEVAANLGVSLSSAGLLVTGYALGVFVGAPLLTATTGR 64 Query: 102 LSRRGLLIGAMTAFTLANLLAWQAGGYWSLMTARILLAFAAGLYVPNALALAGALVAPEK 161 L R+ LL+ M F + NL A Y +LM R++ +FA G + +A +LV EK Sbjct: 65 LPRKTLLVALMVVFVVGNLACAIAPDYTTLMIGRVVASFAHGAFFGVGSVVATSLVPKEK 124 Query: 162 RGSALALVSGGITVAVALAAPLGTVIADSLSWRMTFAGVALLAAIATLGLMLGLPRGIGN 221 + SA+AL+ G+TVA L P GT + S WR TF V ++ IA + +PR G Sbjct: 125 QASAIALMFTGLTVANVLGVPFGTWLGQSYGWRATFWAVTVIGVIALAATAVLVPRSQGG 184 Query: 222 NLPVASLRERVLAARDPAVLRALLTTLLWATGAYTMYTYFALYLNQVTGIHGTHVGYVLF 281 RVL P V+ LL T+L G + +TY A L ++G + V +L Sbjct: 185 EETDFGKEVRVL--MRPQVMLGLLMTVLGFGGVFAAFTYIAPILTDLSGFPASAVSPILL 242 Query: 282 AWGISAAIGVMAGGKIADR 300 +G+ IG +AGGK+AD+ Sbjct: 243 LFGLGLVIGNLAGGKLADK 261 >gi|300310405|ref|YP_003774497.1| arabinose efflux permease [Herbaspirillum seropedicae SmR1] Length = 422 Score = 78.2 bits (191), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 81/253 (32%), Positives = 123/253 (48%), Gaps = 11/253 (4%) Query: 36 AASNAPLYW-----MALGTFAVGAEGFMIAGILPTVAKDLGVSVAVAGQLVVAFTAAYAI 90 A AP W +ALG F++ F+ A +L +A DL VS AGQ V A A+ Sbjct: 28 AGQEAPANWSGIASLALGVFSLVTAEFLPASLLTAIASDLHVSDGAAGQAVTATAIVGAV 87 Query: 91 SSPILTALTGALSRRGLLIGAMTAFTLANLLAWQAGGYWSLMTARILLAFAAGLYVPNAL 150 ++P + LT + R+ +++ ++N+LA A L+ AR+LL A G + A Sbjct: 88 AAPSIPLLTRNIDRKRVVLMLSLLLLVSNILAVTAPNLPVLLVARVLLGVALGGFWSMAA 147 Query: 151 ALAGALVAPEKRGSALALVSGGITVAVALAAPLGTVIADSLSWRMTF--AGVALLAAIAT 208 ALA LV A++L+ G++VA AAP+G + + WR F AGV L AIA Sbjct: 148 ALAMRLVPDHLFARAMSLILTGVSVATVCAAPIGAYMGNLWGWRSAFVAAGVVSLVAIAI 207 Query: 209 LGLMLGLPRGIGNNLPVASLRERVLAARDPAVLRALLTTLLWATGAYTMYTYFALYLNQV 268 + +PR + P + +L +D V ALL LL +G + +TY + Q+ Sbjct: 208 --QWIAIPRLPPKDHPSLKVLAELLGRKD--VRVALLAVLLVVSGHFAGFTYIRPLMEQI 263 Query: 269 TGIHGTHVGYVLF 281 T + T + VL Sbjct: 264 THMSVTTISAVLL 276 >gi|300312017|ref|YP_003776109.1| multidrug resistance B (translocase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 524 Score = 55.5 bits (132), Expect = 9e-09, Method: Compositional matrix adjust. Identities = 73/203 (35%), Positives = 99/203 (48%), Gaps = 21/203 (10%) Query: 25 MNTSSLPASA----PAASNAPLYWMALGTFAVGAEGFM------IAGI-LPTVAKDLGVS 73 M TS P+ A P + A L LGT +V FM IA + +PT++ +LGVS Sbjct: 1 MATSPTPSQAYTPPPPLTGANL---VLGTLSVSLAVFMNVLDSSIANVAIPTISGNLGVS 57 Query: 74 VAVAGQLVVAFTAAYAISSPILTALTGALSRRGLLIGAMTAFTLANLLAWQAGGYWSLMT 133 V ++ +F AA AIS P+ LT L L + ++ F LA+ L A L+ Sbjct: 58 VDEGTWVITSFAAANAISIPLTGWLTQRLGAVRLFVTSVLLFVLASWLCGLAPTLPILLA 117 Query: 134 ARILLAFAAGLYVPNALALAGALVAPEKRGSALALVSGGITVAVA-LAAP-LGTVIADSL 191 AR+L AG VP + +L L A K S+ AL G+T VA +A P LG I DS Sbjct: 118 ARVLQGLVAGPMVPLSQSL--LLGAYPKSKSSFALALWGMTAVVAPVAGPALGGWITDSY 175 Query: 192 SWRMTF---AGVALLAAIATLGL 211 +W F V L+AA TL + Sbjct: 176 TWPWIFYINIPVGLIAAGVTLAI 198 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.137 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,206,452 Number of extensions: 47765 Number of successful extensions: 447 Number of sequences better than 1.0e-08: 12 Number of HSP's gapped: 445 Number of HSP's successfully gapped: 12 Length of query: 432 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 344 Effective length of database: 1,203,190 Effective search space: 413897360 Effective search space used: 413897360 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)