BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0221.p (320 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310169|ref|YP_003774261.1| methyl-accepting chemotaxis tra... 151 5e-38 gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis tra... 81 1e-16 gi|300313253|ref|YP_003777345.1| methyl-accepting chemotaxis I M... 79 5e-16 gi|300310055|ref|YP_003774147.1| methyl-accepting chemotaxis tra... 77 3e-15 gi|300313049|ref|YP_003777141.1| methyl-accepting chemotaxis I p... 77 3e-15 >gi|300310169|ref|YP_003774261.1| methyl-accepting chemotaxis transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 657 Score = 151 bits (382), Expect = 5e-38, Method: Compositional matrix adjust. Identities = 72/146 (49%), Positives = 99/146 (67%), Gaps = 1/146 (0%) Query: 10 QIDKLARRLAGSYPDTFSTDARELLVVGAETTPVLRNGAEVLNLNFGRIDSFSAATGGSA 69 Q+D+ + + S D + +G TPVL+N LNL+F D F+A TG +A Sbjct: 78 QVDRFSTMFDSEFGK-LSLDETRTVQIGERATPVLQNDGHELNLDFSIPDRFTAKTGVTA 136 Query: 70 TIFVQRGKDFVRISTSVKKENGERAIGTLLDHAHAGYRDLLAGRSYTGFAQLFGRQYMTR 129 TIF + G+DFVRISTSVKKENGERAIGTLLD +H GY L + ++Y+G A LFG+QY+T+ Sbjct: 137 TIFAKTGEDFVRISTSVKKENGERAIGTLLDRSHPGYAALKSDKAYSGLATLFGKQYITK 196 Query: 130 YDPVRDAQGRVVAVLYVGIDVSQRFQ 155 Y PV++ G+++A LYVGID+S + Sbjct: 197 YVPVKNNAGQIIAALYVGIDISSDIK 222 Score = 89.7 bits (221), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 54/110 (49%), Positives = 69/110 (62%), Gaps = 6/110 (5%) Query: 209 IALGLLALGM--GGLYWLAQRMVTRPLVDATAAAQQLAKGDLTTMIHVGRRDEIGQLTQA 266 I LGLL L + L + RMV+RPL AA ++A GDLTT +HV DEIG+L A Sbjct: 336 IGLGLLFLAVLAAILTVMVNRMVSRPLDAVCQAADRIASGDLTTRLHVHSSDEIGRLMGA 395 Query: 267 INGVAQGLATIVGAVRGSSERINIAAHEIAAGNDDLSARAEAPRRAQAGA 316 NG++ GL+ +V +VR +E IN AA EIA+GN +LS R E QAGA Sbjct: 396 FNGISMGLSEVVSSVRAGTEEINTAASEIASGNLNLSVRTE----QQAGA 441 >gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 533 Score = 80.9 bits (198), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 54/116 (46%), Positives = 67/116 (57%), Gaps = 8/116 (6%) Query: 201 ALLLHYGAIALGLLALGMGGLYWLAQRMVTRPLVDATAAAQQLAKGDLTTMIHVGRRDEI 260 ++LL GAI + L A L WL R +TRPL A A+ +A GDLT+ I RDE Sbjct: 188 SMLLALGAIEIVLGA----ALAWLLTRSITRPLNQAVTIAETVAAGDLTSRIDSSSRDET 243 Query: 261 GQLTQAINGVAQGLATIVGAVRGSSERINIAAHEIAAGNDDLSARAEAPRRAQAGA 316 G+L A+ + L IVG VR +E IN A+ EIA GN DLSAR E AQAG+ Sbjct: 244 GKLLAALKTMNGNLLDIVGRVRMGTETINTASREIATGNLDLSARTE----AQAGS 295 >gi|300313253|ref|YP_003777345.1| methyl-accepting chemotaxis I MCP-I serine chemoreceptor transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 556 Score = 79.0 bits (193), Expect = 5e-16, Method: Compositional matrix adjust. Identities = 46/104 (44%), Positives = 61/104 (58%), Gaps = 5/104 (4%) Query: 209 IALGLLALGMGGLY-----WLAQRMVTRPLVDATAAAQQLAKGDLTTMIHVGRRDEIGQL 263 I + LLA+G G + WL R +++PL A AQ +A GDL+ I V +DE GQL Sbjct: 193 ILMALLAMGAGLIVAVLEAWLLVRAISQPLTQAVGIAQAVAGGDLSRQIEVRSQDETGQL 252 Query: 264 TQAINGVAQGLATIVGAVRGSSERINIAAHEIAAGNDDLSARAE 307 QA+ + Q L IV VR +E I +A+ EI +GN DLSAR E Sbjct: 253 MQALKDMTQRLYQIVAVVRQGTETITMASKEITSGNLDLSARTE 296 >gi|300310055|ref|YP_003774147.1| methyl-accepting chemotaxis transducer transmembrane protein I [Herbaspirillum seropedicae SmR1] Length = 547 Score = 76.6 bits (187), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 39/89 (43%), Positives = 53/89 (59%) Query: 219 GGLYWLAQRMVTRPLVDATAAAQQLAKGDLTTMIHVGRRDEIGQLTQAINGVAQGLATIV 278 G + WL R ++RPL A + AQ + GDL + I V +DE+GQL A+ G+ L IV Sbjct: 206 GMVAWLLTRSISRPLAQAVSVAQSVQSGDLGSRIEVTSKDELGQLMSALKGMNDSLVNIV 265 Query: 279 GAVRGSSERINIAAHEIAAGNDDLSARAE 307 G VR ++ I +A+ EI GN DLS R E Sbjct: 266 GEVRQGTDTIAVASDEIKQGNMDLSNRTE 294 >gi|300313049|ref|YP_003777141.1| methyl-accepting chemotaxis I protein [Herbaspirillum seropedicae SmR1] Length = 516 Score = 76.6 bits (187), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 42/92 (45%), Positives = 60/92 (65%), Gaps = 1/92 (1%) Query: 216 LGMGGLYWLAQRMVTRPLVDATAAAQQLAKGDLTTMIHVGRRDEIGQLTQAINGVAQGLA 275 +GMG ++A R++ RPL DA A+++A+GDL I V +DE GQL QA++ + +GL Sbjct: 204 IGMGLALYVA-RIIARPLQDAVDIARRVAEGDLGAQIQVQSKDETGQLMQALDTMNEGLR 262 Query: 276 TIVGAVRGSSERINIAAHEIAAGNDDLSARAE 307 +VG VR ++ I A+ EIA GN DLS R E Sbjct: 263 KVVGQVRSGTDSIATASTEIATGNLDLSRRTE 294 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 900,919 Number of extensions: 36919 Number of successful extensions: 262 Number of sequences better than 1.0e-08: 40 Number of HSP's gapped: 274 Number of HSP's successfully gapped: 41 Length of query: 320 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 235 Effective length of database: 1,217,395 Effective search space: 286087825 Effective search space used: 286087825 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)