BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0208.p (206 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313083|ref|YP_003777175.1| hypothetical protein Hsero_3789... 181 4e-47 >gi|300313083|ref|YP_003777175.1| hypothetical protein Hsero_3789 [Herbaspirillum seropedicae SmR1] Length = 196 Score = 181 bits (459), Expect = 4e-47, Method: Compositional matrix adjust. Identities = 99/192 (51%), Positives = 125/192 (65%), Gaps = 15/192 (7%) Query: 25 AAIITEVHILTTTRSKRWNGNIDTLKAPYLAGVITQMGIYGERVQFALVTSGNGPRYQLT 84 A I V IL+++++KRW+GN++ +KA +L GVITQM YG+RV F++ S P Y++ Sbjct: 9 ACFIGRV-ILSSSKTKRWHGNVEAIKAEFLNGVITQMTSYGDRVHFSMFGSVQKPHYEVI 67 Query: 85 NTAGKKMAFDGNHHLHHPEDGEFAPGNATGVFTLDQV-RLAKAGGSKTTRT--------- 134 N+ KKMAFDGNHHLH E+ EF PGN T +FTLDQ+ R+ GGS T T Sbjct: 68 NSFDKKMAFDGNHHLHRAEESEFGPGNVTPIFTLDQIKRIVATGGSGTRSTPRSARAASA 127 Query: 135 LSTGGVKRATTRAPSAATRAKDQVDSDKYEYFKANRATLPDTIGEHSEEITTLMRGGMPA 194 S GG R TT A A VDS+KY YFKANR LPD I +HS+EI+ LM GM A Sbjct: 128 RSAGGTARPTTAAARARE----AVDSEKYAYFKANRHMLPDEINQHSDEISKLMLNGMSA 183 Query: 195 EQAFGEIVKKYF 206 EQAFGE++ KYF Sbjct: 184 EQAFGEVISKYF 195 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.132 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 633,936 Number of extensions: 24249 Number of successful extensions: 72 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 71 Number of HSP's successfully gapped: 1 Length of query: 206 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 125 Effective length of database: 1,236,335 Effective search space: 154541875 Effective search space used: 154541875 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)