BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0205.p (332 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310832|ref|YP_003774924.1| D-isomer specific 2-hydroxyacid... 111 9e-26 gi|300313735|ref|YP_003777827.1| D-3-phosphoglycerate dehydrogen... 106 3e-24 gi|300310960|ref|YP_003775052.1| dehydrogenase oxidoreductase [H... 105 5e-24 gi|300311988|ref|YP_003776080.1| D-3-phosphoglycerate dehydrogen... 90 3e-19 gi|300313156|ref|YP_003777248.1| D-2-hydroxyacid dehydrogenase [... 87 2e-18 >gi|300310832|ref|YP_003774924.1| D-isomer specific 2-hydroxyacid dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 319 Score = 111 bits (277), Expect = 9e-26, Method: Compositional matrix adjust. Identities = 95/278 (34%), Positives = 138/278 (49%), Gaps = 15/278 (5%) Query: 25 LQALGTVRLNDSETPLTPSDLVRAAAGCDIIVAPRIPAATAEVFDHLPQLVAYCRGAVDI 84 LQA + L D T +D + A A ++ +P A + + PQL +V + Sbjct: 19 LQAEFDLTLFDRITDDNRADFIAALATAHGMIGASLPITNA-MLEAAPQLKIVSTISVGV 77 Query: 85 RNIDVAAASRNGVLVTRATPG-FAASVAEWVLAAMFDLSRGISDAALAYR----NGIVPA 139 N D+ + G+++ TPG + A+ + A + +R + + A + G + Sbjct: 78 DNFDLDYFRQRGLMLAH-TPGVLTEATADTIFALILASARRVVELAEYVKAGRWKGSIGE 136 Query: 140 VRMGRELRGATLGVAGYGTIGQYLCRIAQ-ALGMKIVV----ADPYASIIEPSHVAASFE 194 + G + G TLG+ G G IG + R A GM I+ DP A E S E Sbjct: 137 AQFGVNVHGKTLGLIGMGRIGSAVARRAHHGFGMPILYHNRRPDPEAER-ELGARYVSKE 195 Query: 195 AMLAQSDFVVCLAPATAETAQLFNAAAFAKMQASAFFINASRGELVDEAALLQALDHGVI 254 +LAQ+DFV + P T +T ++F A FA M+ SA FINASRG +VDEAAL+ AL I Sbjct: 196 DLLAQADFVCVMLPLTPQTERMFGAPEFALMKRSAIFINASRGRIVDEAALIAALQDKTI 255 Query: 255 LAAALDV-GMAPDQMPSPLLARHPRVIATPHIGGLTPQ 291 A LDV + P + SPLL + P V+A PHIG T + Sbjct: 256 HGAGLDVFEVEPLPLQSPLL-KLPNVVALPHIGSATHE 292 >gi|300313735|ref|YP_003777827.1| D-3-phosphoglycerate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 308 Score = 106 bits (264), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 86/255 (33%), Positives = 122/255 (47%), Gaps = 4/255 (1%) Query: 41 TPSDLVRAAAGCD-IIVAPRIPAATAEVFDHLPQLVAYCRGAVDIRNIDVAAASRNGVLV 99 T D+V+ A + + R +A V D P L + I +ID AA + G+ V Sbjct: 37 TEDDMVKLAQLHQPVAIIVRYGGVSARVMDAAPILRVISKHGTGIDSIDSQAAQQRGIAV 96 Query: 100 TRATPGFAASVAEWVLAAMFDLSRGISDAALAYRNG-IVPAVRMGRELRGATLGVAGYGT 158 A A +VAE A + ++ + R G + EL+G TLG+ G G Sbjct: 97 KAAAGANAPAVAEHTWALIMACAKNVVGLDQRMREGHWDKSTHKSLELQGRTLGLVGLGA 156 Query: 159 IGQYLCRIAQALGMKIVVADPYASIIEPSHVAASFEAMLAQSDFVVCLAPATAETAQLFN 218 IG+ + +A ALGM ++ DPYA + + AQSD V P TAE + N Sbjct: 157 IGRRVAAVAAALGMPVLAHDPYAKEAPQGVQLVDLDTLFAQSDVVSLHCPLTAENKHMIN 216 Query: 219 AAAFAKMQASAFFINASRGELVDEAALLQALDHGVILAAALD-VGMAPDQMPSPLLARHP 277 A + A+M+ A +N +RG L+DE AL+ ALD G + AA LD P P P L R Sbjct: 217 AQSLARMKDGAILVNTARGGLIDEQALIAALDSGKLRAAGLDSFEKEPFTAPHP-LQRVG 275 Query: 278 RVIATPHIGGLTPQA 292 + +PHIGG+T A Sbjct: 276 NAVLSPHIGGVTSDA 290 >gi|300310960|ref|YP_003775052.1| dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 326 Score = 105 bits (262), Expect = 5e-24, Method: Compositional matrix adjust. Identities = 98/311 (31%), Positives = 144/311 (46%), Gaps = 16/311 (5%) Query: 22 LALLQALGTVRLNDSETPLTPSDLVRAAAGCD-IIVAPRIPAATAEVFDHLPQLVAYCRG 80 LA L+ V N + + ++L A G D + P P TA +F PQL A C Sbjct: 16 LARLEQHFEVESNQDDRIYSAAELQAKAQGKDGLFTTPSEPV-TAALFAANPQLKAVCNM 74 Query: 81 AVDIRNIDVAAASRNGVLVTRATPGFAASVAEWVLAAMFDLSRGISDAALAYRNGIVPAV 140 AV NIDVAAA++ GV+ T + A++ A M +R I+++ R G Sbjct: 75 AVGYNNIDVAAATQAGVMATNTPDVLNETTADFGWALMMAAARRITESEHFLRAGKWKKW 134 Query: 141 R----MGRELRGATLGVAGYGTIGQYLCRIAQALGMKIVVADPYASIIEPSHVA------ 190 +G ++ GATLG+ G G IGQ + R ++LG + V S + P A Sbjct: 135 SYDSFVGPDIHGATLGIIGMGRIGQAIAR--RSLGFDMQVLYHNRSRLAPELEARANHAR 192 Query: 191 -ASFEAMLAQSDFVVCLAPATAETAQLFNAAAFAKMQASAFFINASRGELVDEAALLQAL 249 S E +L Q+D V+ + P + +T AA A M+ +A N +RG +VD+AAL+ AL Sbjct: 193 YVSKEELLRQADHVILVLPYSKDTHHTIAAAELALMKPTATLTNIARGGIVDDAALIAAL 252 Query: 250 DHGVILAAALDVGMAPDQMPSPLLARHPRVIATPHIGGLTPQAAEHQAMDTVHQVAAILA 309 G I AA +DV + P V+ TPHI + A + A L+ Sbjct: 253 REGRIAAAGVDV-FENEPAFKPEFLDLSNVVLTPHIASASTPTRLAMANCAADNLIAALS 311 Query: 310 GRLPQGAVNAD 320 G+ P +N D Sbjct: 312 GQRPPNLLNPD 322 >gi|300311988|ref|YP_003776080.1| D-3-phosphoglycerate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 310 Score = 89.7 bits (221), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 67/230 (29%), Positives = 100/230 (43%), Gaps = 2/230 (0%) Query: 64 TAEVFDHLPQLVAYCRGAVDIRNIDVAAASRNGVLVTRATPGFAASVAEWVLAAMFDLSR 123 TAE LP L C N+DVA A +G+ + VA+ L + + R Sbjct: 57 TAEEMRALPHLQLVCTLGAGFENVDVAHAEAHGIEIATGAGTNEDCVADHALGLLLAILR 116 Query: 124 GISDAALAYRNG-IVPAVRMGRELRGATLGVAGYGTIGQYLCRIAQALGMKIVVADPYAS 182 I R+G + + +L G +G+ G G IG+ + R A A +I + Sbjct: 117 NIPVLDRYTRDGGWRETIPLQPQLAGKRVGIVGMGNIGKKIARRAAAFDAEIAYCNRKKR 176 Query: 183 IIEPSHVAASFEAMLAQSDFVVCLAPATAETAQLFNAAAFAKMQASAFFINASRGELVDE 242 H + +D ++ AP A+T L NA ++ + +N RG +VD Sbjct: 177 DDVDYHYFPDVAQLAGWADCLIVAAPGGAQTRHLINARVLEELGPQGYLVNIGRGSIVDT 236 Query: 243 AALLQALDHGVILAAALDVGMAPDQMPSPLLARHPRVIATPHIGGLTPQA 292 AL AL G + A LDV Q P+ L+A P V+ TPHI G +P+A Sbjct: 237 DALGAALSSGRLAGAGLDVYEGEPQPPAALIAL-PNVVLTPHIAGWSPEA 285 >gi|300313156|ref|YP_003777248.1| D-2-hydroxyacid dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 316 Score = 87.4 bits (215), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 71/225 (31%), Positives = 102/225 (45%), Gaps = 10/225 (4%) Query: 66 EVFDHLPQLVAYCRGAVDIRNIDVAAASRNGVLVTRATPGFAASVAEWVLAAMFDLSRGI 125 E P L V +DV AA G+ VT VA+ +A M +R + Sbjct: 63 EFMARFPALEIVSVFGVGYDGVDVPAARERGIHVTHTPDVLNDDVADLAMALMLATARNV 122 Query: 126 SDA-----ALAYRNGIVPAVRMGRELRGATLGVAGYGTIGQYLCRIAQALGMKIVVADPY 180 A + ++ G P ++ GA LG+ G G IGQ + + A A M+I + Sbjct: 123 VRADRFARSGEWKKGPFP---FTTKVSGARLGIVGLGRIGQAIAKRAAAFDMQISYHNRS 179 Query: 181 ASIIEPSHVAASFEAMLAQSDFVVCLAPATAETAQLFNAAAFAKMQASAFFINASRGELV 240 + ++V S A+ + DF+V + P A T L NA + F IN +RG +V Sbjct: 180 RKDVPYTYVD-SITALAREVDFLVMITPGGAGTRALVNAEVLEALGPKGFLINVARGSVV 238 Query: 241 DEAALLQALDHGVILAAALDVGMAPDQMPSPLLARHPRVIATPHI 285 DEAAL+ AL GVI A LDV +P+ L A V+ TPH+ Sbjct: 239 DEAALIAALKTGVIAGAGLDVFADEPNVPAELAALD-NVVLTPHM 282 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.133 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,048,214 Number of extensions: 45056 Number of successful extensions: 206 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 188 Number of HSP's successfully gapped: 11 Length of query: 332 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 246 Effective length of database: 1,212,660 Effective search space: 298314360 Effective search space used: 298314360 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)