BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0198.p (197 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313080|ref|YP_003777172.1| major facilitator superfamily p... 193 7e-51 >gi|300313080|ref|YP_003777172.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 403 Score = 193 bits (491), Expect = 7e-51, Method: Compositional matrix adjust. Identities = 108/190 (56%), Positives = 128/190 (67%), Gaps = 17/190 (8%) Query: 6 VWRVAMAIGILCFPQVSVLTFATIFLHDVGHAGLGLISATMAAIQIGAAVMRVWSGRWTD 65 VWR+A+ IG+LC PQV++L+F IFL + A + IS + +Q+GAA R+ SGRWTD Sbjct: 230 VWRLALGIGLLCAPQVALLSFGGIFLQESAQASVLAISVLLTLVQLGAAAARIASGRWTD 289 Query: 66 RHGNRRRYLRACSALSVALYIALGTVAALAPAVWPQWTLAAIALLLVAGGVCASAWHGVA 125 R G RR YLR C+ S AL+ G + LAP LLVA GV SAWHGVA Sbjct: 290 RRGQRRDYLRGCALASAALF---GLLGVLAP-------------LLVAAGVVVSAWHGVA 333 Query: 126 YTELATLAGPRQVGTALGLGNTCVFLVFFLAAQAVPALLAWQSWPAVWLAGAAGALIAWP 185 YTELA +AG QVGTALGLGN+CVFLVFFLAAQA+P LL WQ WPAVWLA A AL+ WP Sbjct: 334 YTELAVVAGAGQVGTALGLGNSCVFLVFFLAAQAIPPLLHWQGWPAVWLAIAVVALLVWP 393 Query: 186 IFLKPLHRNR 195 +F P R R Sbjct: 394 LF-PPEDRAR 402 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.137 0.461 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 543,071 Number of extensions: 19361 Number of successful extensions: 143 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 142 Number of HSP's successfully gapped: 1 Length of query: 197 Length of database: 1,619,870 Length adjustment: 80 Effective length of query: 117 Effective length of database: 1,241,070 Effective search space: 145205190 Effective search space used: 145205190 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 128 (53.9 bits)